DNASU Plasmid | TMEM208 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TMEM208

Gene Name: transmembrane protein 208

Original Clone ID:

ccsbBroadEn_03079

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC003080.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00506615

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 520nts

Open reading frame: 1 to 520

Reference Sequence Alignment

ATGGCGCCCAAGGGCAAAGTGGGCACGAGAGGGAAGAAGCAGATATTTGAAGAGAACAGA
GAGACTCTGAAGTTCTACCTGCGGATCATACTGGGGGCCAATGCCATTTACTGCCTTGTG
ACGTTGGTCTTCTTTTACTCATCTGCCTCATTTTGGGCCTGGTTGGCCCTGGGCTTTAGT
CTGGCAGTGTATGGGGCCAGCTACCACTCTATGAGCTCGATGGCACGAGCAGCGTTCTCT
GAGGATGGGGCCCTGATGGATGGTGGCATGGACCTCAACATGGAGCAGGGCATGGCAGAG
CACCTTAAGGATGTGATCCTACTGACAGCCATCGTGCAGGTGCTCAGCTGCTTCTCTCTC
TATGTCTGGTCCTTCTGGCTTCTGGCTCCAGGCCGGGCCCTTTACCTCCTGTGGGTGAAT
GTGCTGGGCCCCTGGTTCACTGCAGACAGTGGCACCCCAGCACCAGAGCACAATGAGAAA
CGGCAGCGCCGACAGGAGCGGCGGCAGATGAAGCGGTTA

Protein sequence predicted based on nucleotide sequence

MAPKGKVGTRGKKQIFEENRETLKFYLRIILGANAIYCLVTLVFFYSSASFWAWLALGFS
LAVYGASYHSMSSMARAAFSEDGALMDGGMDLNMEQGMAEHLKDVILLTAIVQVLSCFSL
YVWSFWLLAPGRALYLLWVNVLGPWFTADSGTPAPEHNEKRQRRQERRQMKRL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

TMEM208

Symbol Nomenclature :

TMEM208

Full Nomenclature :

transmembrane protein 208

GENEID :

29100

Locus Tag :

HSPC171

GenBank Accession :

DQ895786

HGNC :

25015

Vega :

OTTHUMG00000173064

Target GenBank:

DQ895786

Reference Sequence Alignment

  

HsCD00506615      ATGGCGCCCAAGGGCAAAGTGGGCACGAGAGGGAAGAAGCAGATATTTGAAGAGAACAGA
NM_014187.3       ATGGCGCCCAAGGGCAAAGTGGGCACGAGAGGGAAGAAGCAGATATTTGAAGAGAACAGA
                  ************************************************************

HsCD00506615      GAGACTCTGAAGTTCTACCTGCGGATCATACTGGGGGCCAATGCCATTTACTGCCTTGTG
NM_014187.3       GAGACTCTGAAGTTCTACCTGCGGATCATACTGGGGGCCAATGCCATTTACTGCCTTGTG
                  ************************************************************

HsCD00506615      ACGTTGGTCTTCTTTTACTCATCTGCCTCATTTTGGGCCTGGTTGGCCCTGGGCTTTAGT
NM_014187.3       ACGTTGGTCTTCTTTTACTCATCTGCCTCATTTTGGGCCTGGTTGGCCCTGGGCTTTAGT
                  ************************************************************

HsCD00506615      CTGGCAGTGTATGGGGCCAGCTACCACTCTATGAGCTCGATGGCACGAGCAGCGTTCTCT
NM_014187.3       CTGGCAGTGTATGGGGCCAGCTACCACTCTATGAGCTCGATGGCACGAGCAGCGTTCTCT
                  ************************************************************

HsCD00506615      GAGGATGGGGCCCTGATGGATGGTGGCATGGACCTCAACATGGAGCAGGGCATGGCAGAG
NM_014187.3       GAGGATGGGGCCCTGATGGATGGTGGCATGGACCTCAACATGGAGCAGGGCATGGCAGAG
                  ************************************************************

HsCD00506615      CACCTTAAGGATGTGATCCTACTGACAGCCATCGTGCAGGTGCTCAGCTGCTTCTCTCTC
NM_014187.3       CACCTTAAGGATGTGATCCTACTGACAGCCATCGTGCAGGTGCTCAGCTGCTTCTCTCTC
                  ************************************************************

HsCD00506615      TATGTCTGGTCCTTCTGGCTTCTGGCTCCAGGCCGGGCCCTTTACCTCCTGTGGGTGAAT
NM_014187.3       TATGTCTGGTCCTTCTGGCTTCTGGCTCCAGGCCGGGCCCTTTACCTCCTGTGGGTGAAT
                  ************************************************************

HsCD00506615      GTGCTGGGCCCCTGGTTCACTGCAGACAGTGGCACCCCAGCACCAGAGCACAATGAGAAA
NM_014187.3       GTGCTGGGCCCCTGGTTCACTGCAGACAGTGGCACCCCAGCACCAGAGCACAATGAGAAA
                  ************************************************************

HsCD00506615      CGGCAGCGCCGACAGGAGCGGCGGCAGATGAAGCGGTTA---
NM_014187.3       CGGCAGCGCCGACAGGAGCGGCGGCAGATGAAGCGGTTATAG
                  ***************************************