DNASU Plasmid | TPPP (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TPPP

Gene Name: tubulin polymerization promoting protein

Original Clone ID:

ccsbBroadEn_02614

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC131506.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00507376

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 658nts

Open reading frame: 1 to 658

Reference Sequence Alignment

ATGGCTGACAAGGCCAAGCCTGCCAAAGCTGCCAACAGGACGCCCCCCAAGTCCCCGGGG
GACCCCTCGAAGGACCGGGCAGCCAAGAGGCTGTCGCTGGAATCGGAGGGTGCTGGTGAG
GGGGCAGCCGCATCCCCTGAGCTCAGTGCCCTGGAGGAGGCCTTCCGGCGCTTTGCCGTG
CACGGGGACGCCAGGGCCACCGGGAGGGAGATGCACGGCAAGAACTGGTCGAAGCTGTGC
AAGGACTGCCAGGTGATCGACGGCAGGAACGTGACCGTCACTGACGTGGACATCGTCTTC
AGCAAGATCAAAGGGAAGTCTTGCCGGACCATCACCTTTGAGCAGTTCCAGGAGGCGCTG
GAGGAGCTCGCCAAGAAGCGATTCAAAGACAAGAGCAGCGAGGAGGCCGTTCGCGAGGTG
CACAGGCTCATCGAGGGCAAGGCGCCCATCATCTCAGGGGTGACGAAAGCCATCTCGTCG
CCCACAGTGTCGAGGCTCACGGACACCACCAAGTTCACGGGCTCCCACAAGGAGCGCTTC
GACCCCTCTGGCAAGGGCAAGGGCAAGGCTGGCCGCGTGGATCTGGTGGACGAGTCAGGC
TATGTGTCCGGCTACAAGCACGCAGGCACCTACGACCAGAAGGTGCAAGGGGGCAAG

Protein sequence predicted based on nucleotide sequence

MADKAKPAKAANRTPPKSPGDPSKDRAAKRLSLESEGAGEGAAASPELSALEEAFRRFAV
HGDARATGREMHGKNWSKLCKDCQVIDGRNVTVTDVDIVFSKIKGKSCRTITFEQFQEAL
EELAKKRFKDKSSEEAVREVHRLIEGKAPIISGVTKAISSPTVSRLTDTTKFTGSHKERF
DPSGKGKGKAGRVDLVDESGYVSGYKHAGTYDQKVQGGK

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

TPPP

Symbol Nomenclature :

TPPP

SYNONYM :

TPPP/p25; TPPP1; p24; p25; p25alpha

Designation :

25 kDa brain-specific protein|brain specific protein p25 alpha|glycogen synthase kinase 3 (GSK3) inhibitor p24|p25-alpha|tubulin polymerization-promoting protein

Full Nomenclature :

tubulin polymerization promoting protein

GENEID :

11076

HGNC :

24164

MIM :

608773

Vega :

OTTHUMG00000131011

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00507376        ------------------------------------------------------------
XM_005248237.1      ATGAAGTCACTGCCCGCAGCAGGTTTTGAGAGATCCCTGGCCACAGAAACGGCCCATCTC
NM_007030.2         ------------------------------------------------------------
                                                                                

HsCD00507376        ------------------------------------------------------------
XM_005248237.1      AGGGTCCTGACACCACCGCGTCCTGGCAGGTGGGGCTGTCAGACCGCCCCCCGCAGAGCC
NM_007030.2         ------------------------------------------------------------
                                                                                

HsCD00507376        ------------------------------------------------------------
XM_005248237.1      TCCAGCTGCCCCGGGTCTTGGCTACTGTCCGCGGGCCTGTGGCGATGGAGAGGCGCTGCC
NM_007030.2         ------------------------------------------------------------
                                                                                

HsCD00507376        ------------------------------------------------------------
XM_005248237.1      GGGACACCTTCCCGCACCCCTGGGAGCAGCGCCCGAAGATCTGAGGAGCCCTTTATTCTG
NM_007030.2         ------------------------------------------------------------
                                                                                

HsCD00507376        ------------------------------------ATGGCTGACAAGGCCAAGCCTGCC
XM_005248237.1      CGGTTTCGGTTACGTAAACGGAGCCGAGGGAGCAACATGGCTGACAAGGCCAAGCCTGCC
NM_007030.2         ------------------------------------ATGGCTGACAAGGCCAAGCCTGCC
                                                        ************************

HsCD00507376        AAAGCTGCCAACAGGACGCCCCCCAAGTCCCCGGGGGACCCCTCGAAGGACCGGGCAGCC
XM_005248237.1      AAAGCTGCCAACAGGACGCCCCCCAAGTCCCCGGGGGACCCCTCGAAGGACCGGGCAGCC
NM_007030.2         AAAGCTGCCAACAGGACGCCCCCCAAGTCCCCGGGGGACCCCTCGAAGGACCGGGCAGCC
                    ************************************************************

HsCD00507376        AAGAGGCTGTCGCTGGAATCGGAGGGTGCTGGTGAGGGGGCAGCCGCATCCCCTGAGCTC
XM_005248237.1      AAGAGGCTGTCGCTGGAATCGGAGGGTGCTGGTGAGGGGGCAGCCGCATCCCCTGAGCTC
NM_007030.2         AAGAGGCTGTCGCTGGAATCGGAGGGTGCTGGTGAGGGGGCAGCCGCATCCCCTGAGCTC
                    ************************************************************

HsCD00507376        AGTGCCCTGGAGGAGGCCTTCCGGCGCTTTGCCGTGCACGGGGACGCCAGGGCCACCGGG
XM_005248237.1      AGTGCCCTGGAGGAGGCCTTCCGGCGCTTTGCCGTGCACGGGGACGCCAGGGCCACCGGG
NM_007030.2         AGTGCCCTGGAGGAGGCCTTCCGGCGCTTTGCCGTGCACGGGGACGCCAGGGCCACCGGG
                    ************************************************************

HsCD00507376        AGGGAGATGCACGGCAAGAACTGGTCGAAGCTGTGCAAGGACTGCCAGGTGATCGACGGC
XM_005248237.1      AGGGAGATGCACGGCAAGAACTGGTCGAAGCTGTGCAAGGACTGCCAGGTGATCGACGGC
NM_007030.2         AGGGAGATGCACGGCAAGAACTGGTCGAAGCTGTGCAAGGACTGCCAGGTGATCGACGGC
                    ************************************************************

HsCD00507376        AGGAACGTGACCGTCACTGACGTGGACATCGTCTTCAGCAAGATCAAAGGGAAGTCTTGC
XM_005248237.1      AGGAACGTGACCGTCACTGACGTGGACATCGTCTTCAGCAAGATCAAAGGGAAGTCTTGC
NM_007030.2         AGGAACGTGACCGTCACTGACGTGGACATCGTCTTCAGCAAGATCAAAGGGAAGTCTTGC
                    ************************************************************

HsCD00507376        CGGACCATCACCTTTGAGCAGTTCCAGGAGGCGCTGGAGGAGCTCGCCAAGAAGCGATTC
XM_005248237.1      CGGACCATCACCTTTGAGCAGTTCCAGGAGGCGCTGGAGGAGCTCGCCAAGAAGCGATTC
NM_007030.2         CGGACCATCACCTTTGAGCAGTTCCAGGAGGCGCTGGAGGAGCTCGCCAAGAAGCGATTC
                    ************************************************************

HsCD00507376        AAAGACAAGAGCAGCGAGGAGGCCGTTCGCGAGGTGCACAGGCTCATCGAGGGCAAGGCG
XM_005248237.1      AAAGACAAGAGCAGCGAGGAGGCCGTTCGCGAGGTGCACAGGCTCATCGAGGGCAAGGCG
NM_007030.2         AAAGACAAGAGCAGCGAGGAGGCCGTTCGCGAGGTGCACAGGCTCATCGAGGGCAAGGCG
                    ************************************************************

HsCD00507376        CCCATCATCTCAGGGGTGACGAAAGCCATCTCGTCGCCCACAGTGTCGAGGCTCACGGAC
XM_005248237.1      CCCATCATCTCAGGGGTGACGAAAGCCATCTCGTCGCCCACAGTGTCGAGGCTCACGGAC
NM_007030.2         CCCATCATCTCAGGGGTGACGAAAGCCATCTCGTCGCCCACAGTGTCGAGGCTCACGGAC
                    ************************************************************

HsCD00507376        ACCACCAAGTTCACGGGCTCCCACAAGGAGCGCTTCGACCCCTCTGGCAAGGGCAAGGGC
XM_005248237.1      ACCACCAAGTTCACGGGCTCCCACAAGGAGCGCTTCGACCCCTCTGGCAAGGGCAAGGGC
NM_007030.2         ACCACCAAGTTCACGGGCTCCCACAAGGAGCGCTTCGACCCCTCTGGCAAGGGCAAGGGC
                    ************************************************************

HsCD00507376        AAGGCTGGCCGCGTGGATCTGGTGGACGAGTCAGGCTATGTGTCCGGCTACAAGCACGCA
XM_005248237.1      AAGGCTGGCCGCGTGGATCTGGTGGACGAGTCAGGCTATGTGTCCGGCTACAAGCACGCA
NM_007030.2         AAGGCTGGCCGCGTGGATCTGGTGGACGAGTCAGGCTATGTGTCCGGCTACAAGCACGCA
                    ************************************************************

HsCD00507376        GGCACCTACGACCAGAAGGTGCAAGGGGGCAAG---
XM_005248237.1      GGCACCTACGACCAGAAGGTGCAAGGGGGCAAGTAG
NM_007030.2         GGCACCTACGACCAGAAGGTGCAAGGGGGCAAGTAG
                    *********************************