DNASU Plasmid | WDR37 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: WDR37

Gene Name: WD repeat domain 37

Original Clone ID:

ccsbBroadEn_11641

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC018044.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00508554

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 793nts

Open reading frame: 1 to 793

Reference Sequence Alignment

ATGCCCACAGAAAGCGCAAGTTGTTCGACTACTCGCCAAACAAAACAGAAGCGCAAATCC
CATAGCCTTTCTATACGAAGAACTAACAGCTCGGAGCAGGAGAGGACGGGACTGCCAAGA
GACATGTTAGAAGGACAAGATTCTAAACTGCCTTCCTCGGTTCGCAGTACACTTCTGGAA
CTGTTTGGTCAAATAGAAAGAGAATTTGAAAACCTTTATATCGAAAACTTAGAATTACGT
AGAGAAATCGACACTCTTAATGAACGTTTAGCTGCTGAAGGACAAGCGATTGATGGAGCA
GAGCTGAGTAAGGGCCAACTCAAAACAAAAGCCAGTCACAGCACCAGCCAGCTCTCCCAG
AAACTGAAGACCACTTACAAGGCTTCCACCAGCAAGATTGTCTCCAGCTTTAAGACCACG
ACATCGAGAGCTGCCTGCCAGCTCGTGAAGGAGTACATCGGCCACCGGGACGGCATCTGG
GATGTCAGCGTGGCCAAGACACAGCCAGTGGTGCTCGGGACTGCATCAGCCGATCACACG
GCTTTGCTGTGGAGCATAGAGACAGGGAAGTGCCTAGTCAAGTACGCAGGCCACGTGGGC
TCAGTAAATTCTATCAAATTTCATCCCTCAGAGCAGTTGGCTCTCACTGCTTCTGGAGAT
AAGACTGCTCATATCTGGAGATACGCGGTGCAGCTGCCGACACCCCAGCCTGTTGCTGAC
ACTAGTGTAAGCACCTTTCCTTACCTGCGAATGTGTAGGATGCTGATGTCTGCGAATCTG
AGAATCCATTTA

Protein sequence predicted based on nucleotide sequence

MPTESASCSTTRQTKQKRKSHSLSIRRTNSSEQERTGLPRDMLEGQDSKLPSSVRSTLLE
LFGQIEREFENLYIENLELRREIDTLNERLAAEGQAIDGAELSKGQLKTKASHSTSQLSQ
KLKTTYKASTSKIVSSFKTTTSRAACQLVKEYIGHRDGIWDVSVAKTQPVVLGTASADHT
ALLWSIETGKCLVKYAGHVGSVNSIKFHPSEQLALTASGDKTAHIWRYAVQLPTPQPVAD
TSVSTFPYLRMCRMLMSANLRIHL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

WDR37

Symbol Nomenclature :

WDR37

SYNONYM :

RP11-529L18.2

Designation :

WD repeat-containing protein 37

Full Nomenclature :

WD repeat domain 37

GENEID :

22884

GenBank Accession :

AM392677

HGNC :

31406

Vega :

OTTHUMG00000017540

Target GenBank:

AM392677

Reference Sequence Alignment

  

HsCD00508554      ATGCCCACAGAAAGCGCAAGTTGTTCGACTACTCGCCAAACAAAACAGAAGCGCAAATCC
NM_014023.3       ATGCCCACAGAAAGCGCAAGTTGTTCGACTGCTCGCCAAACAAAACAGAAGCGCAAATCC
                  ******************************.*****************************

HsCD00508554      CATAGCCTTTCTATACGAAGAACTAACAGCTCGGAGCAGGAGAGGACGGGACTGCCAAGA
NM_014023.3       CATAGCCTTTCTATACGAAGAACTAACAGCTCGGAGCAGGAGAGGACGGGACTGCCAAGA
                  ************************************************************

HsCD00508554      GACATGTTAGAAGGACAAGATTCTAAACTGCCTTCCTCGGTTCGCAGTACACTTCTGGAA
NM_014023.3       GACATGTTAGAAGGACAAGATTCTAAACTGCCTTCCTCGGTTCGCAGTACACTTCTGGAA
                  ************************************************************

HsCD00508554      CTGTTTGGTCAAATAGAAAGAGAATTTGAAAACCTTTATATCGAAAACTTAGAATTACGT
NM_014023.3       CTGTTTGGTCAAATAGAAAGAGAATTTGAAAACCTTTATATCGAAAACTTAGAATTACGT
                  ************************************************************

HsCD00508554      AGAGAAATCGACACTCTTAATGAACGTTTAGCTGCTGAAGGACAAGCGATTGATGGAGCA
NM_014023.3       AGAGAAATCGACACTCTTAATGAACGTTTAGCTGCTGAAGGACAAGCGATTGATGGAGCA
                  ************************************************************

HsCD00508554      GAGCTGAGTAAGGGCCAACTCAAAACAAAAGCCAGTCACAGCACCAGCCAGCTCTCCCAG
NM_014023.3       GAGCTGAGTAAGGGCCAACTCAAAACAAAAGCCAGTCACAGCACCAGCCAGCTCTCCCAG
                  ************************************************************

HsCD00508554      AAACTGAAGACCACTTACAAGGCTTCCACCAGCAAGATTGTCTCCAGCTTTAAGACCACG
NM_014023.3       AAACTGAAGACCACTTACAAGGCTTCCACCAGCAAGATTGTCTCCAGCTTTAAGACCACG
                  ************************************************************

HsCD00508554      ACATCGAGAGCTGCCTGCCAGCTCGTGAAGGAGTACATCGGCCACCGGGACGGCATCTGG
NM_014023.3       ACATCGAGAGCTGCCTGCCAGCTCGTGAAGGAGTACATCGGCCACCGGGACGGCATCTGG
                  ************************************************************

HsCD00508554      GATGTCAGCGTGGCCAAGACACAGCCAGTGGTGCTCGGGACTGCATCAGCCGATCACACG
NM_014023.3       GATGTCAGCGTGGCCAAGACACAGCCAGTGGTGCTCGGGACTGCATCAGCCGATCACACG
                  ************************************************************

HsCD00508554      GCTTTGCTGTGGAGCATAGAGACAGGGAAGTGCCTAGTCAAGTACGCAGGCCACGTGGGC
NM_014023.3       GCTTTGCTGTGGAGCATAGAGACAGGGAAGTGCCTAGTCAAGTACGCAGGCCACGTGGGC
                  ************************************************************

HsCD00508554      TCAGTAAATTCTATCAAATTTCATCCCTCAGAGCAGTTGGCTCTCACTGCTTCTGGAGAT
NM_014023.3       TCAGTAAATTCTATCAAATTTCATCCCTCAGAGCAGTTGGCTCTCACTGCTTCTGGAGAT
                  ************************************************************

HsCD00508554      AAGACTGCTCATATCTGGAGATACGCGGTGCAGCTGCCGACACCCCAGCCTGTTGCTGAC
NM_014023.3       CAGACTGCTCATATCTGGAGATACGCGGTGCAGCTGCCGACACCCCAGCCTGTTGCTGAC
                  .***********************************************************

HsCD00508554      ACTAGTGTAAGCACCTTTCCTTACCTGCGAATGTGTAGGATGCTGATGTCTGCGAATCTG
NM_014023.3       ACTAGTATATCTGGGGAAGATGAAGTAGAGTGCTCTGACAAGGACGAGCCCGACCTCGAT
                  ******.**:  .   :: .* *. *. ..:  * *.. *:* : .:* * *. .:  : 

HsCD00508554      AGAATCCATTTA------------------------------------------------
NM_014023.3       GGGGATGTGTCCAGCGACTGCCCCACCATCCGCGTCCCACTGACATCCCTCAAGAGCCAC
                  .*..:  : * .                                                

HsCD00508554      ------------------------------------------------------------
NM_014023.3       CAGGGCGTGGTCATCGCCTCCGACTGGCTGGTTGGGGGGAAGCAGGCTGTGACTGCCTCC
                                                                              

HsCD00508554      ------------------------------------------------------------
NM_014023.3       TGGGACCGGACGGCAAACCTGTACGACGTGGAGACGTCCGAGCTCGTTCACTCTCTGACA
                                                                              

HsCD00508554      ------------------------------------------------------------
NM_014023.3       GGGCACGACCAGGAGTTGACGCACTGCTGCACACACCCCACCCAGCGGCTCGTGGTGACC
                                                                              

HsCD00508554      ------------------------------------------------------------
NM_014023.3       TCCTCCCGTGACACGACTTTCCGCCTCTGGGATTTCAGGGACCCCTCCATCCACTCGGTG
                                                                              

HsCD00508554      ------------------------------------------------------------
NM_014023.3       AATGTTTTCCAGGGACACACGGACACTGTGACTTCTGCCGTGTTCACCGTGGGAGACAAC
                                                                              

HsCD00508554      ------------------------------------------------------------
NM_014023.3       GTGGTTTCAGGCAGCGATGACCGCACGGTGAAAGTCTGGGACTTGAAAAATATGAGATCC
                                                                              

HsCD00508554      ------------------------------------------------------------
NM_014023.3       CCCATTGCAACTATTCGCACGGACTCTGCCATTAACAGGATCAATGTATGTGTCGGCCAA
                                                                              

HsCD00508554      ------------------------------------------------------------
NM_014023.3       AAAATCATAGCCCTCCCCCATGACAACCGACAAGTGAGACTGTTTGATATGTCAGGAGTG
                                                                              

HsCD00508554      ------------------------------------------------------------
NM_014023.3       CGCCTGGCGCGGCTTCCCCGGAGCAGCCGACAGGGCCACAGGAGAATGGTATGCTGCTCG
                                                                              

HsCD00508554      ------------------------------------------------------------
NM_014023.3       GCATGGAGTGAAGACCACCCCGTGTGCAATCTGTTCACCTGTGGGTTTGACCGGCAAGCC
                                                                              

HsCD00508554      ---------------------------------------------
NM_014023.3       ATTGGTTGGAACATCAACATCCCTGCATTGCTACAAGAAAAATAA