DNASU Plasmid | TAF1D (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TAF1D

Gene Name: TATA box binding protein (TBP)-associated factor, RNA polymerase I, D, 41kDa

Original Clone ID:

ccsbBroadEn_04051

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC001972.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00508732

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 835nts

Open reading frame: 1 to 835

Reference Sequence Alignment

ATGGATAAATCAGGAATAGATTCTCTTGACCATGTGACATCTGATGCTGTGGAACTTGCA
AATCGAAGTGATAACTCTTCTGATAGCAGCTTATTTAAAACTCAGTGTATCCCTTACTCA
CCTAAAGGGGAGAAAAGAAACCCCATTCGAAAATTTGTTCGTACACCTGAAAGTGTTCAC
GCAAGTGATTCATCAAGTGACTCATCTTTTGAACCAATACCATTGACTATAAAAGCTATT
TTTGAAAGATTCAAGAACAGGAAAAAGAGATATAAAAAAAAGAAAAAGAGGAGGTACCAG
CCAACAGGAAGACCACGGGGAAGACCAGAAGGAAGGAGAAATCCTATATACTCACTAATA
GATAAGAAGAAACAATTTAGAAGCAGAGGATCTGGCTTCCCATTTTTAGAATCAGAGAAT
GAAAAAAACGCACCTTGGAGAAAAATTTTAACGTTTGAGCAAGCTGTTGCAAGAGGATTT
TTTAACTATATTGAAAAACTGAAGTATGAACACCACCTGAAAGAATCATTGAAGCAAATG
AATGTTGGTGAAGATTTAGAAAATGAAGATTTTGACAGTCGTAGATACAAATTTTTGGAT
GATGATGGATCCATTTCTCCTATTGAGGAGTCAACAGCAGAGGATGAGGATGCAACACAT
CTTGAAGATAACGAATGTGATATCAAATTGGCAGGGGATAGTTTCATAGTAAGTTCTGAA
TTCCCTGTAAGACTGAGTGTATACTTAGAAGAAGAGGATATTACTGAAGAAGCTGCTTTG
TCTAAAAAGAGAGCTACAAAAGCCAAAAATACTGGACAGAGAGGCCTGAAAATG

Protein sequence predicted based on nucleotide sequence

MDKSGIDSLDHVTSDAVELANRSDNSSDSSLFKTQCIPYSPKGEKRNPIRKFVRTPESVH
ASDSSSDSSFEPIPLTIKAIFERFKNRKKRYKKKKKRRYQPTGRPRGRPEGRRNPIYSLI
DKKKQFRSRGSGFPFLESENEKNAPWRKILTFEQAVARGFFNYIEKLKYEHHLKESLKQM
NVGEDLENEDFDSRRYKFLDDDGSISPIEESTAEDEDATHLEDNECDIKLAGDSFIVSSE
FPVRLSVYLEEEDITEEAALSKKRATKAKNTGQRGLKM

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TAF1D

Symbol Nomenclature :

TAF1D

SYNONYM :

JOSD3; RAFI41; TAF(I)41; TAFI41

Designation :

Josephin domain containing 3|RNA polymerase I-specific TBP-associated factor 41 kDa|TATA box-binding protein-associated factor 1D|TATA box-binding protein-associated factor RNA polymerase I subunit D|TBP-associated factor 1D|transcription initiation factor SL1/TIF-IB subunit D

Full Nomenclature :

TATA box binding protein (TBP)-associated factor, RNA polymerase I, D, 41kDa

GENEID :

79101

GenBank Accession :

HQ447355

HGNC :

28759

MIM :

612823

Vega :

OTTHUMG00000167451

Target GenBank:

HQ447355

Reference Sequence Alignment

  

HsCD00508732      ATGGATAAATCAGGAATAGATTCTCTTGACCATGTGACATCTGATGCTGTGGAACTTGCA
NM_024116.3       ATGGATAAATCAGGAATAGATTCTCTTGACCATGTGACATCTGATGCTGTGGAACTTGCA
                  ************************************************************

HsCD00508732      AATCGAAGTGATAACTCTTCTGATAGCAGCTTATTTAAAACTCAGTGTATCCCTTACTCA
NM_024116.3       AATCGAAGTGATAACTCTTCTGATAGCAGCTTATTTAAAACTCAGTGTATCCCTTACTCA
                  ************************************************************

HsCD00508732      CCTAAAGGGGAGAAAAGAAACCCCATTCGAAAATTTGTTCGTACACCTGAAAGTGTTCAC
NM_024116.3       CCTAAAGGGGAGAAAAGAAACCCCATTCGAAAATTTGTTCGTACACCTGAAAGTGTTCAC
                  ************************************************************

HsCD00508732      GCAAGTGATTCATCAAGTGACTCATCTTTTGAACCAATACCATTGACTATAAAAGCTATT
NM_024116.3       GCAAGTGATTCATCAAGTGACTCATCTTTTGAACCAATACCATTGACTATAAAAGCTATT
                  ************************************************************

HsCD00508732      TTTGAAAGATTCAAGAACAGGAAAAAGAGATATAAAAAAAAGAAAAAGAGGAGGTACCAG
NM_024116.3       TTTGAAAGATTCAAGAACAGGAAAAAGAGATATAAAAAAAAGAAAAAGAGGAGGTACCAG
                  ************************************************************

HsCD00508732      CCAACAGGAAGACCACGGGGAAGACCAGAAGGAAGGAGAAATCCTATATACTCACTAATA
NM_024116.3       CCAACAGGAAGACCACGGGGAAGACCAGAAGGAAGGAGAAATCCTATATACTCACTAATA
                  ************************************************************

HsCD00508732      GATAAGAAGAAACAATTTAGAAGCAGAGGATCTGGCTTCCCATTTTTAGAATCAGAGAAT
NM_024116.3       GATAAGAAGAAACAATTTAGAAGCAGAGGATCTGGCTTCCCATTTTTAGAATCAGAGAAT
                  ************************************************************

HsCD00508732      GAAAAAAACGCACCTTGGAGAAAAATTTTAACGTTTGAGCAAGCTGTTGCAAGAGGATTT
NM_024116.3       GAAAAAAACGCACCTTGGAGAAAAATTTTAACGTTTGAGCAAGCTGTTGCAAGAGGATTT
                  ************************************************************

HsCD00508732      TTTAACTATATTGAAAAACTGAAGTATGAACACCACCTGAAAGAATCATTGAAGCAAATG
NM_024116.3       TTTAACTATATTGAAAAACTGAAGTATGAACACCACCTGAAAGAATCATTGAAGCAAATG
                  ************************************************************

HsCD00508732      AATGTTGGTGAAGATTTAGAAAATGAAGATTTTGACAGTCGTAGATACAAATTTTTGGAT
NM_024116.3       AATGTTGGTGAAGATTTAGAAAATGAAGATTTTGACAGTCGTAGATACAAATTTTTGGAT
                  ************************************************************

HsCD00508732      GATGATGGATCCATTTCTCCTATTGAGGAGTCAACAGCAGAGGATGAGGATGCAACACAT
NM_024116.3       GATGATGGATCCATTTCTCCTATTGAGGAGTCAACAGCAGAGGATGAGGATGCAACACAT
                  ************************************************************

HsCD00508732      CTTGAAGATAACGAATGTGATATCAAATTGGCAGGGGATAGTTTCATAGTAAGTTCTGAA
NM_024116.3       CTTGAAGATAACGAATGTGATATCAAATTGGCAGGGGATAGTTTCATAGTAAGTTCTGAA
                  ************************************************************

HsCD00508732      TTCCCTGTAAGACTGAGTGTATACTTAGAAGAAGAGGATATTACTGAAGAAGCTGCTTTG
NM_024116.3       TTCCCTGTAAGACTGAGTGTATACTTAGAAGAAGAGGATATTACTGAAGAAGCTGCTTTG
                  ************************************************************

HsCD00508732      TCTAAAAAGAGAGCTACAAAAGCCAAAAATACTGGACAGAGAGGCCTGAAAATG---
NM_024116.3       TCTAAAAAGAGAGCTACAAAAGCCAAAAATACTGGACAGAGAGGCCTGAAAATGTGA
                  ******************************************************

Pathways

Reactome :

Epigenetic regulation of gene expression

Reactome :

Gene Expression

Reactome :

Negative epigenetic regulation of rRNA expression

Reactome :

NoRC negatively regulates rRNA expression

Reactome :

RNA Polymerase I Chain Elongation

Reactome :

RNA Polymerase I Promoter Clearance

Reactome :

RNA Polymerase I Promoter Escape

Reactome :

RNA Polymerase I Transcription

Reactome :

RNA Polymerase I Transcription Initiation

Reactome :

RNA Polymerase I Transcription Termination

Reactome :

RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription

Reactome :

SIRT1 negatively regulates rRNA Expression

Reactome :

Transcription

Protein

HPRD :

14679

Clone

Cloning Information :

613387

Original Clone ID :

ccsbBroadEn_04051

Organism

Species Specific ID:

79101

Genome

Chromosome :

Map Location :

11q21

Ensembl :

ENSG00000166012

Reagents

Labome :

MGC5306-antibody