DNASU Plasmid | BUB3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: BUB3

Gene Name: BUB3 mitotic checkpoint protein

Original Clone ID:

ccsbBroadEn_02100

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC005138.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00509338

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 985nts

Open reading frame: 1 to 985

Reference Sequence Alignment

ATGACCGGTTCTAACGAGTTCAAGCTGAACCAGCCACCCGAGGATGGCATCTCCTCCGTG
AAGTTCAGCCCCAACACCTCCCAGTTCCTGCTTGTCTCCTCCTGGGACACGTCCGTGCGT
CTCTACGATGTGCCGGCCAACTCCATGCGGCTCAAGTACCAGCACACCGGCGCCGTCCTG
GACTGCGCCTTCTACGATCCAACGCATGCCTGGAGTGGAGGACTAGATCATCAATTGAAA
ATGCATGATTTGAACACTGATCAAGAAAATCTTGTTGGGACCCATGATGCCCCTATCAGA
TGTGTTGAATACTGTCCAGAAGTGAATGTGATGGTCACTGGAAGTTGGGATCAGACAGTT
AAACTGTGGGATCCCAGAACTCCTTGTAATGCTGGGACCTTCTCTCAGCCTGAAAAGGTA
TATACCCTCTCAGTGTCTGGAGACCGGCTGATTGTGGGAACAGCAGGCCGCAGAGTGTTG
GTGTGGGACTTACGGAACATGGGTTACGTGCAGCAGCGCAGGGAGTCCAGCCTGAAATAC
CAGACTCGCTGCATACGAGCGTTTCCAAACAAGCAGGGTTATGTATTAAGCTCTATTGAA
GGCCGAGTGGCAGTTGAGTATTTGGACCCAAGCCCTGAGGTACAGAAGAAGAAGTATGCC
TTCAAATGTCACAGACTAAAAGAAAATAATATTGAGCAGATTTACCCAGTCAATGCCATT
TCTTTTCACAATATCCACAATACATTTGCCACAGGTGGTTCTGATGGCTTTGTAAATATT
TGGGATCCATTTAACAAAAAGCGACTGTGCCAATTCCATCGGTACCCCACGAGCATCGCA
TCACTTGCCTTCAGTAATGATGGGACTACGCTTGCAATAGCGTCATCATATATGTATGAA
ATGGATGACACAGAACATCCTGAAGATGGTATCTTCATTCGCCAAGTGACAGATGCAGAA
ACAAAACCCAAGTCACCATGTACT

Protein sequence predicted based on nucleotide sequence

MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVL
DCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTV
KLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKY
QTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAI
SFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYE
MDDTEHPEDGIFIRQVTDAETKPKSPCT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

BUB3

Symbol Nomenclature :

BUB3

SYNONYM :

BUB3L; hBUB3

Designation :

BUB3 budding uninhibited by benzimidazoles 3 homolog|budding uninhibited by benomyl|budding uninhibited by benzimidazoles 3 homolog|mitotic checkpoint component|mitotic checkpoint protein BUB3

Full Nomenclature :

BUB3 mitotic checkpoint protein

GENEID :

9184

GenBank Accession :

DQ895042

HGNC :

1151

MIM :

603719

Vega :

OTTHUMG00000019197

Target GenBank:

DQ895042

Reference Sequence Alignment

  

HsCD00509338        ATGACCGGTTCTAACGAGTTCAAGCTGAACCAGCCACCCGAGGATGGCATCTCCTCCGTG
NM_004725.3         ATGACCGGTTCTAACGAGTTCAAGCTGAACCAGCCACCCGAGGATGGCATCTCCTCCGTG
NM_001007793.2      ATGACCGGTTCTAACGAGTTCAAGCTGAACCAGCCACCCGAGGATGGCATCTCCTCCGTG
                    ************************************************************

HsCD00509338        AAGTTCAGCCCCAACACCTCCCAGTTCCTGCTTGTCTCCTCCTGGGACACGTCCGTGCGT
NM_004725.3         AAGTTCAGCCCCAACACCTCCCAGTTCCTGCTTGTCTCCTCCTGGGACACGTCCGTGCGT
NM_001007793.2      AAGTTCAGCCCCAACACCTCCCAGTTCCTGCTTGTCTCCTCCTGGGACACGTCCGTGCGT
                    ************************************************************

HsCD00509338        CTCTACGATGTGCCGGCCAACTCCATGCGGCTCAAGTACCAGCACACCGGCGCCGTCCTG
NM_004725.3         CTCTACGATGTGCCGGCCAACTCCATGCGGCTCAAGTACCAGCACACCGGCGCCGTCCTG
NM_001007793.2      CTCTACGATGTGCCGGCCAACTCCATGCGGCTCAAGTACCAGCACACCGGCGCCGTCCTG
                    ************************************************************

HsCD00509338        GACTGCGCCTTCTACGATCCAACGCATGCCTGGAGTGGAGGACTAGATCATCAATTGAAA
NM_004725.3         GACTGCGCCTTCTACGATCCAACGCATGCCTGGAGTGGAGGACTAGATCATCAATTGAAA
NM_001007793.2      GACTGCGCCTTCTACGATCCAACGCATGCCTGGAGTGGAGGACTAGATCATCAATTGAAA
                    ************************************************************

HsCD00509338        ATGCATGATTTGAACACTGATCAAGAAAATCTTGTTGGGACCCATGATGCCCCTATCAGA
NM_004725.3         ATGCATGATTTGAACACTGATCAAGAAAATCTTGTTGGGACCCATGATGCCCCTATCAGA
NM_001007793.2      ATGCATGATTTGAACACTGATCAAGAAAATCTTGTTGGGACCCATGATGCCCCTATCAGA
                    ************************************************************

HsCD00509338        TGTGTTGAATACTGTCCAGAAGTGAATGTGATGGTCACTGGAAGTTGGGATCAGACAGTT
NM_004725.3         TGTGTTGAATACTGTCCAGAAGTGAATGTGATGGTCACTGGAAGTTGGGATCAGACAGTT
NM_001007793.2      TGTGTTGAATACTGTCCAGAAGTGAATGTGATGGTCACTGGAAGTTGGGATCAGACAGTT
                    ************************************************************

HsCD00509338        AAACTGTGGGATCCCAGAACTCCTTGTAATGCTGGGACCTTCTCTCAGCCTGAAAAGGTA
NM_004725.3         AAACTGTGGGATCCCAGAACTCCTTGTAATGCTGGGACCTTCTCTCAGCCTGAAAAGGTA
NM_001007793.2      AAACTGTGGGATCCCAGAACTCCTTGTAATGCTGGGACCTTCTCTCAGCCTGAAAAGGTA
                    ************************************************************

HsCD00509338        TATACCCTCTCAGTGTCTGGAGACCGGCTGATTGTGGGAACAGCAGGCCGCAGAGTGTTG
NM_004725.3         TATACCCTCTCAGTGTCTGGAGACCGGCTGATTGTGGGAACAGCAGGCCGCAGAGTGTTG
NM_001007793.2      TATACCCTCTCAGTGTCTGGAGACCGGCTGATTGTGGGAACAGCAGGCCGCAGAGTGTTG
                    ************************************************************

HsCD00509338        GTGTGGGACTTACGGAACATGGGTTACGTGCAGCAGCGCAGGGAGTCCAGCCTGAAATAC
NM_004725.3         GTGTGGGACTTACGGAACATGGGTTACGTGCAGCAGCGCAGGGAGTCCAGCCTGAAATAC
NM_001007793.2      GTGTGGGACTTACGGAACATGGGTTACGTGCAGCAGCGCAGGGAGTCCAGCCTGAAATAC
                    ************************************************************

HsCD00509338        CAGACTCGCTGCATACGAGCGTTTCCAAACAAGCAGGGTTATGTATTAAGCTCTATTGAA
NM_004725.3         CAGACTCGCTGCATACGAGCGTTTCCAAACAAGCAGGGTTATGTATTAAGCTCTATTGAA
NM_001007793.2      CAGACTCGCTGCATACGAGCGTTTCCAAACAAGCAGGGTTATGTATTAAGCTCTATTGAA
                    ************************************************************

HsCD00509338        GGCCGAGTGGCAGTTGAGTATTTGGACCCAAGCCCTGAGGTACAGAAGAAGAAGTATGCC
NM_004725.3         GGCCGAGTGGCAGTTGAGTATTTGGACCCAAGCCCTGAGGTACAGAAGAAGAAGTATGCC
NM_001007793.2      GGCCGAGTGGCAGTTGAGTATTTGGACCCAAGCCCTGAGGTACAGAAGAAGAAGTATGCC
                    ************************************************************

HsCD00509338        TTCAAATGTCACAGACTAAAAGAAAATAATATTGAGCAGATTTACCCAGTCAATGCCATT
NM_004725.3         TTCAAATGTCACAGACTAAAAGAAAATAATATTGAGCAGATTTACCCAGTCAATGCCATT
NM_001007793.2      TTCAAATGTCACAGACTAAAAGAAAATAATATTGAGCAGATTTACCCAGTCAATGCCATT
                    ************************************************************

HsCD00509338        TCTTTTCACAATATCCACAATACATTTGCCACAGGTGGTTCTGATGGCTTTGTAAATATT
NM_004725.3         TCTTTTCACAATATCCACAATACATTTGCCACAGGTGGTTCTGATGGCTTTGTAAATATT
NM_001007793.2      TCTTTTCACAATATCCACAATACATTTGCCACAGGTGGTTCTGATGGCTTTGTAAATATT
                    ************************************************************

HsCD00509338        TGGGATCCATTTAACAAAAAGCGACTGTGCCAATTCCATCGGTACCCCACGAGCATCGCA
NM_004725.3         TGGGATCCATTTAACAAAAAGCGACTGTGCCAATTCCATCGGTACCCCACGAGCATCGCA
NM_001007793.2      TGGGATCCATTTAACAAAAAGCGACTGTGCCAATTCCATCGGTACCCCACGAGCATCGCA
                    ************************************************************

HsCD00509338        TCACTTGCCTTCAGTAATGATGGGACTACGCTTGCAATAGCGTCATCATATATGTATGAA
NM_004725.3         TCACTTGCCTTCAGTAATGATGGGACTACGCTTGCAATAGCGTCATCATATATGTATGAA
NM_001007793.2      TCACTTGCCTTCAGTAATGATGGGACTACGCTTGCAATAGCGTCATCATATATGTATGAA
                    ************************************************************

HsCD00509338        ATGGATGACACAGAACATCCTGAAGATGGTATCTTCATTCGCCAAGTGACAGATGCAGAA
NM_004725.3         ATGGATGACACAGAACATCCTGAAGATGGTATCTTCATTCGCCAAGTGACAGATGCAGAA
NM_001007793.2      ATGGATGACACAGAACATCCTGAAGATGGTATCTTCATTCGCCAAGTGACAGATGCAGAA
                    ************************************************************

HsCD00509338        ACAAAACCCAAGTCACCATGTACT---
NM_004725.3         ACAAAACCCAAGTCACCATGTACTTGA
NM_001007793.2      ACAAAACCCAAGTCCACCTAA------
                    **************..*.*.:

Pathways

NCI :

p73 transcription factor network

Reactome :

Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins

Reactome :

APC-Cdc20 mediated degradation of Nek2A

Reactome :

APC/C-mediated degradation of cell cycle proteins

Reactome :

APC/C:Cdc20 mediated degradation of mitotic proteins

Reactome :

Cdc20:Phospho-APC/C mediated degradation of Cyclin A

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

Inactivation of APC/C via direct inhibition of the APC/C complex

Reactome :

Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components

Reactome :

M Phase

Reactome :

Mitotic Anaphase

Reactome :

Mitotic M-M/G1 phases

Reactome :

Mitotic Metaphase and Anaphase

Reactome :

Mitotic Prometaphase

Reactome :

Mitotic Spindle Checkpoint

Reactome :

Regulation of APC/C activators between G1/S and early anaphase

Reactome :

Regulation of mitotic cell cycle

Reactome :

Resolution of Sister Chromatid Cohesion

Reactome :

Separation of Sister Chromatids

WikiPathway :

Cell cycle

Protein

SMART domain :

WD40 : WD40 repeats

HPRD :

04761

Clone

Cloning Information :

613524

Original Clone ID :

ccsbBroadEn_02100

Organism

Species Specific ID:

9184

Genome

Chromosome :

Map Location :

10q26

Ensembl :

ENSG00000154473

Reagents

Labome :

BUB3-antibody