DNASU Plasmid | SLC25A3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: SLC25A3

Gene Name: solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3

Original Clone ID:

ccsbBroadEn_01187

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC000998.2,BC001328.2,BC003504.1,BC004345.1,BC006455.1,BC011574.1,BC011641.2,BC014019.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00510291

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1083nts

Open reading frame: 1 to 1083

Reference Sequence Alignment

ATGTTCTCGTCCGTGGCGCACCTGGCGCGGGCGAACCCCTTCAACACGCCACATCTGCAG
CTGGTGCACGATGGTCTCGGGGACCTCCGCAGCAGCTCCCCAGGGCCCACGGGCCAGCCC
CGCCGCCCTCGCAACCTGGCAGCCGCCGCCGTGGAAGAGTACAGTTGTGAATTTGGCTCC
GCGAAGTATTATGCACTGTGTGGCTTTGGTGGGGTCTTAAGTTGTGGTCTGACACACACT
GCTGTGGTTCCCCTGGATTTAGTGAAATGCCGTATGCAGGTGGACCCCCAAAAGTACAAG
GGCATATTTAACGGATTCTCAGTTACACTTAAAGAGGATGGTGTTCGTGGTTTGGCTAAA
GGATGGGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCTTTTAT
GAAGTCTTTAAAGTCTTGTATAGCAATATGCTTGGAGAGGAGAATACTTATCTCTGGCGC
ACATCACTATATTTGGCTGCCTCTGCCAGTGCTGAATTCTTTGCTGACATTGCCCTGGCT
CCTATGGAAGCTGCTAAGGTTCGAATTCAAACCCAGCCAGGTTATGCCAACACTTTGAGG
GATGCAGCTCCCAAAATGTATAAGGAAGAAGGCCTAAAAGCATTCTACAAGGGGGTTGCT
CCTCTCTGGATGAGACAGATACCATACACCATGATGAAGTTCGCCTGCTTTGAACGTACT
GTTGAAGCACTGTACAAGTTTGTGGTTCCTAAGCCCCGCAGTGAATGTTCAAAGCCAGAG
CAGCTGGTTGTAACATTTGTAGCAGGTTACATAGCTGGAGTCTTTTGTGCAATTGTTTCT
CACCCTGCTGATTCTGTGGTATCTGTGTTGAATAAAGAAAAAGGTAGCAGTGCTTCTCTG
GTCCTCAAGAGACTTGGATTTAAAGGTGTATGGAAGGGACTGTTTGCCCGTATCATCATG
ATTGGTACCCTGACTGCACTACAGTGGTTTATCTATGACTCCGTGAAGGTCTACTTCAGA
CTTCCTCGCCCTCCTCCACCCGAGATGCCAGAGTCTCTGAAGAAGAAGCTTGGGTTAACT
CAG

Protein sequence predicted based on nucleotide sequence

MFSSVAHLARANPFNTPHLQLVHDGLGDLRSSSPGPTGQPRRPRNLAAAAVEEYSCEFGS
AKYYALCGFGGVLSCGLTHTAVVPLDLVKCRMQVDPQKYKGIFNGFSVTLKEDGVRGLAK
GWAPTFLGYSMQGLCKFGFYEVFKVLYSNMLGEENTYLWRTSLYLAASASAEFFADIALA
PMEAAKVRIQTQPGYANTLRDAAPKMYKEEGLKAFYKGVAPLWMRQIPYTMMKFACFERT
VEALYKFVVPKPRSECSKPEQLVVTFVAGYIAGVFCAIVSHPADSVVSVLNKEKGSSASL
VLKRLGFKGVWKGLFARIIMIGTLTALQWFIYDSVKVYFRLPRPPPPEMPESLKKKLGLT
Q

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

SLC25A3

Symbol Nomenclature :

SLC25A3

SYNONYM :

PHC; PTP

Designation :

mitochondrial phosphate carrier protein|phosphate carrier protein, mitochondrial|phosphate transport protein|solute carrier family 25 member 3

Full Nomenclature :

solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3

GENEID :

5250

Locus Tag :

OK/SW-cl.48

GenBank Accession :

EU176545

HGNC :

10989

MIM :

600370

Vega :

OTTHUMG00000170212

Target GenBank:

EU176545

Reference Sequence Alignment

  

HsCD00510291      ATGTTCTCGTCCGTGGCGCACCTGGCGCGGGCGAACCCCTTCAACACGCCACATCTGCAG
NM_005888.3       ATGTTCTCGTCCGTGGCGCACCTGGCGCGGGCGAACCCCTTCAACACGCCACATCTGCAG
NM_002635.3       ATGTTCTCGTCCGTGGCGCACCTGGCGCGGGCGAACCCCTTCAACACGCCACATCTGCAG
NM_213611.2       ATGTTCTCGTCCGTGGCGCACCTGGCGCGGGCGAACCCCTTCAACACGCCACATCTGCAG
                  ************************************************************

HsCD00510291      CTGGTGCACGATGGTCTCGGGGACCTCCGCAGCAGCTCCCCAGGGCCCACGGGCCAGCCC
NM_005888.3       CTGGTGCACGATGGTCTCGGGGACCTCCGCAGCAGCTCCCCAGGGCCCACGGGCCAGCCC
NM_002635.3       CTGGTGCACGATGGTCTCGGGGACCTCCGCAGCAGCTCCCCAGGGCCCACGGGCCAGCCC
NM_213611.2       CTGGTGCACGATGGTCTCGGGGACCTCCGCAGCAGCTCCCCAGGGCCCACGGGCCAGCCC
                  ************************************************************

HsCD00510291      CGCCGCCCTCGCAACCTGGCAGCCGCCGCCGTG---GAAGAGTACAGTTGTGAATTTGGC
NM_005888.3       CGCCGCCCTCGCAACCTGGCAGCCGCCGCCGTGGAAGAGCAGTATAGCTGTGACTATGGA
NM_002635.3       CGCCGCCCTCGCAACCTGGCAGCCGCCGCCGTG---GAAGAGTACAGTTGTGAATTTGGC
NM_213611.2       CGCCGCCCTCGCAACCTGGCAGCCGCCGCCGTG---GAAGAGTACAGTTGTGAATTTGGC
                  *********************************   **. **** ** *****.*:***.

HsCD00510291      TCCGCGAAGTATTATGCACTGTGTGGCTTTGGTGGGGTCTTAAGTTGTGGTCTGACACAC
NM_005888.3       TCTGGCAGATTCTTTATCCTTTGTGGACTTGGAGGAATTATTAGCTGTGGCACAACACAT
NM_002635.3       TCCGCGAAGTATTATGCACTGTGTGGCTTTGGTGGGGTCTTAAGTTGTGGTCTGACACAC
NM_213611.2       TCCGCGAAGTATTATGCACTGTGTGGCTTTGGTGGGGTCTTAAGTTGTGGTCTGACACAC
                  ** *  *..*: *:*. .** *****. ****:**..* :*:** ***** . .***** 

HsCD00510291      ACTGCTGTGGTTCCCCTGGATTTAGTGAAATGCCGTATGCAGGTGGACCCCCAAAAGTAC
NM_005888.3       ACAGCATTGGTTCCTCTAGATCTGGTTAAATGCAGAATGCAGGTGGACCCCCAAAAGTAC
NM_002635.3       ACTGCTGTGGTTCCCCTGGATTTAGTGAAATGCCGTATGCAGGTGGACCCCCAAAAGTAC
NM_213611.2       ACTGCTGTGGTTCCCCTGGATTTAGTGAAATGCCGTATGCAGGTGGACCCCCAAAAGTAC
                  **:**: ******* **.*** *.** ******.*:************************

HsCD00510291      AAGGGCATATTTAACGGATTCTCAGTTACACTTAAAGAGGATGGTGTTCGTGGTTTGGCT
NM_005888.3       AAGGGCATATTTAACGGATTCTCAGTTACACTTAAAGAGGATGGTGTTCGTGGTTTGGCT
NM_002635.3       AAGGGCATATTTAACGGATTCTCAGTTACACTTAAAGAGGATGGTGTTCGTGGTTTGGCT
NM_213611.2       AAGGGCATATTTAACGGATTCTCAGTTACACTTAAAGAGGATGGTGTTCGTGGTTTGGCT
                  ************************************************************

HsCD00510291      AAAGGATGGGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCTTT
NM_005888.3       AAAGGATGGGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCTTT
NM_002635.3       AAAGGATGGGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCTTT
NM_213611.2       AAAGGATGGGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCTTT
                  ************************************************************

HsCD00510291      TATGAAGTCTTTAAAGTCTTGTATAGCAATATGCTTGGAGAGGAGAATACTTATCTCTGG
NM_005888.3       TATGAAGTCTTTAAAGTCTTGTATAGCAATATGCTTGGAGAGGAGAATACTTATCTCTGG
NM_002635.3       TATGAAGTCTTTAAAGTCTTGTATAGCAATATGCTTGGAGAGGAGAATACTTATCTCTGG
NM_213611.2       TATGAAGTCTTTAAAGTCTTGTATAGCAATATGCTTGGAGAGGAGAATACTTATCTCTGG
                  ************************************************************

HsCD00510291      CGCACATCACTATATTTGGCTGCCTCTGCCAGTGCTGAATTCTTTGCTGACATTGCCCTG
NM_005888.3       CGCACATCACTATATTTGGCTGCCTCTGCCAGTGCTGAATTCTTTGCTGACATTGCCCTG
NM_002635.3       CGCACATCACTATATTTGGCTGCCTCTGCCAGTGCTGAATTCTTTGCTGACATTGCCCTG
NM_213611.2       CGCACATCACTATATTTGGCTGCCTCTGCCAGTGCTGAATTCTTTGCTGACATTGCCCTG
                  ************************************************************

HsCD00510291      GCTCCTATGGAAGCTGCTAAGGTTCGAATTCAAACCCAGCCAGGTTATGCCAACACTTTG
NM_005888.3       GCTCCTATGGAAGCTGCTAAGGTTCGAATTCAAACCCAGCCAGGTTATGCCAACACTTTG
NM_002635.3       GCTCCTATGGAAGCTGCTAAGGTTCGAATTCAAACCCAGCCAGGTTATGCCAACACTTTG
NM_213611.2       GCTCCTATGGAAGCTGCTAAGGTTCGAATTCAAACCCAGCCAGGTTATGCCAACACTTTG
                  ************************************************************

HsCD00510291      AGGGATGCAGCTCCCAAAATGTATAAGGAAGAAGGCCTAAAAGCATTCTACAAGGGGGTT
NM_005888.3       AGGGATGCAGCTCCCAAAATGTATAAGGAAGAAGGCCTAAAAGCATTCTACAAGGGGGTT
NM_002635.3       AGGGATGCAGCTCCCAAAATGTATAAGGAAGAAGGCCTAAAAGCATTCTACAAGGGGGTT
NM_213611.2       AGGGATGCAGCTCCCAAAATGTATAAGGAAGAAGGCCTAAAAGCATTCTACAAGGGGGTT
                  ************************************************************

HsCD00510291      GCTCCTCTCTGGATGAGACAGATACCATACACCATGATGAAGTTCGCCTGCTTTGAACGT
NM_005888.3       GCTCCTCTCTGGATGAGACAGATACCATACACCATGATGAAGTTCGCCTGCTTTGAACGT
NM_002635.3       GCTCCTCTCTGGATGAGACAGATACCATACACCATGATGAAGTTCGCCTGCTTTGAACGT
NM_213611.2       GCTCCTCTCTGGATGAGACAGATACCATACACCATGATGAAGTTCGCCTGCTTTGAACGT
                  ************************************************************

HsCD00510291      ACTGTTGAAGCACTGTACAAGTTTGTGGTTCCTAAGCCCCGCAGTGAATGTTCAAAGCCA
NM_005888.3       ACTGTTGAAGCACTGTACAAGTTTGTGGTTCCTAAGCCCCGCAGTGAATGTTCAAAGCCA
NM_002635.3       ACTGTTGAAGCACTGTACAAGTTTGTGGTTCCTAAGCCCCGCAGTGAATGTTCAAAGCCA
NM_213611.2       ACTGTTGAAGCACTGTACAAGTTTGTGGTTCCTAAGCCCCGCAGTGAATGTTCAAAGCCA
                  ************************************************************

HsCD00510291      GAGCAGCTGGTTGTAACATTTGTAGCAGGTTACATAGCTGGAGTCTTTTGTGCAATTGTT
NM_005888.3       GAGCAGCTGGTTGTAACATTTGTAGCAGGTTACATAGCTGGAGTCTTTTGTGCAATTGTT
NM_002635.3       GAGCAGCTGGTTGTAACATTTGTAGCAGGTTACATAGCTGGAGTCTTTTGTGCAATTGTT
NM_213611.2       GAGCAGCTGGTTGTAACATTTGTAGCAGGTTACATAGCTGGAGTCTTTTGTGCAATTGTT
                  ************************************************************

HsCD00510291      TCTCACCCTGCTGATTCTGTGGTATCTGTGTTGAATAAAGAAAAAGGTAGCAGTGCTTCT
NM_005888.3       TCTCACCCTGCTGATTCTGTGGTATCTGTGTTGAATAAAGAAAAAGGTAGCAGTGCTTCT
NM_002635.3       TCTCACCCTGCTGATTCTGTGGTATCTGTGTTGAATAAAGAAAAAGGTAGCAGTGCTTCT
NM_213611.2       TCTCACCCTGCTGATTCTGTGGTATCTGTGTTGAATAAAGAAAAAGGTAGCAGTGCTTCT
                  ************************************************************

HsCD00510291      CTGGTCCTCAAGAGACTTGGATTTAAAGGTGTATGGAAGGGACTGTTTGCCCGTATCATC
NM_005888.3       CTGGTCCTCAAGAGACTTGGATTTAAAGGTGTATGGAAGGGACTGTTTGCCCGTATCATC
NM_002635.3       CTGGTCCTCAAGAGACTTGGATTTAAAGGTGTATGGAAGGGACTGTTTGCCCGTATCATC
NM_213611.2       CTGGTCCTCAAGAGACTTGGATTTAAAGGTGTATGGAAGGGACTGTTTGCCCGTATCATC
                  ************************************************************

HsCD00510291      ATGATTGGTACCCTGACTGCACTACAGTGGTTTATCTATGACTCCGTGAAGGTCTACTTC
NM_005888.3       ATGATTGGTACCCTGACTGCACTACAGTGGTTTATCTATGACTCCGTGAAGGTCTACTTC
NM_002635.3       ATGATTGGTACCCTGACTGCACTACAGTGGTTTATCTATGACTCCGTGAAGGTCTACTTC
NM_213611.2       ATGATTGGTACCCTGACTGCACTACAGTGGTTTATCTATGACTCCGTGAAGGTCTACTTC
                  ************************************************************

HsCD00510291      AGACTTCCTCGCCCTCCTCCACCCGAGATGCCAGAGTCTCTGAAGAAGAAGCTTGGGTTA
NM_005888.3       AGACTTCCTCGCCCTCCTCCACCCGAGATGCCAGAGTCTCTGAAGAAGAAGCTTGGGTTA
NM_002635.3       AGACTTCCTCGCCCTCCTCCACCCGAGATGCCAGAGTCTCTGAAGAAGAAGCTTGGGTTA
NM_213611.2       AGACTTCCTCGCCCTCCTCCACCCGAGATGCCAGAGTCTCTGAAGAAGAAGCTTGGGTTA
                  ************************************************************

HsCD00510291      ACTCAG---
NM_005888.3       ACTCAGTAG
NM_002635.3       ACTCAGTAG
NM_213611.2       ACTCAGTAG
                  ******