DNASU Plasmid | ARPC1A (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ARPC1A

Gene Name: actin related protein 2/3 complex, subunit 1A, 41kDa

Original Clone ID:

ccsbBroadEn_02466

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC039594.1,BC047889.1,BC054027.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00510409

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1111nts

Open reading frame: 1 to 1111

Reference Sequence Alignment

ATGTCACTGCATCAGTTTTTACTAGAGCCAATCACCTGTCATGCCTGGAACAGGGATCGT
ACTCAGATTGCCCTCAGTCCCAATAATCACGAAGTGCACATCTATAAGAAGAACGGGAGC
CAGTGGGTGAAAGCTCATGAACTCAAGGAGCACAACGGACACATCACAGGTATTGACTGG
GCTCCCAAGAGCGACCGCATTGTCACTTGTGGGGCAGACCGCAATGCCTATGTCTGGAGT
CAGAAAGATGGTGTTTGGAAGCCAACCCTGGTGATCCTGAGAATTAATCGCGCAGCTACT
TTTGTGAAGTGGTCCCCCCTAGAGAACAAATTTGCTGTGGGAAGTGGAGCACGACTCATT
TCTGTTTGTTACTTTGAGTCTGAAAATGACTGGTGGGTGAGCAAGCACATTAAAAAGCCG
ATTCGCTCCACAGTCCTCAGCTTGGATTGGCATCCCAACAACGTTTTGCTGGCAGCAGGA
TCATGTGACTTCAAATGCAGAGTGTTTTCTGCCTACATTAAAGAAGTGGATGAAAAGCCA
GCCAGCACGCCCTGGGGCAGCAAGATGCCTTTTGGGCAGCTGATGTCAGAGTTTGGTGGC
AGTGGCACTGGTGGCTGGGTCCACGGGGTAAGCTTCTCTGCCAGTGGGAGCCGCCTGGCC
TGGGTCAGCCACGACAGCACCGTGTCTGTTGCTGATGCCTCAAAAAGTGTGCAGGTCTCG
ACTCTGAAGACAGAGTTCCTGCCGCTCCTAAGTGTGTCATTTGTCTCAGAGAACAGCGTC
GTGGCTGCTGGCCATGACTGCTGCCCAATGCTCTTTAACTACGATGACCGCGGCTGCCTG
ACCTTCGTCTCCAAGTTAGATATTCCAAAACAGAGCATCCAACGCAACATGTCTGCCATG
GAACGCTTCCGCAACATGGACAAGAGAGCCACAACTGAGGACCGCAACACGGCCTTGGAG
ACGCTGCACCAGAATAGCATCACTCAAGTCTCTATTTATGAGGTGGACAAGCAAGATTGT
CGCAAATTTTGCACTACTGGCATCGATGGAGCCATGACAATTTGGGATTTCAAGACCCTC
GAGTCTTCCATCCAGGGCCTCCGGATAATG

Protein sequence predicted based on nucleotide sequence

MSLHQFLLEPITCHAWNRDRTQIALSPNNHEVHIYKKNGSQWVKAHELKEHNGHITGIDW
APKSDRIVTCGADRNAYVWSQKDGVWKPTLVILRINRAATFVKWSPLENKFAVGSGARLI
SVCYFESENDWWVSKHIKKPIRSTVLSLDWHPNNVLLAAGSCDFKCRVFSAYIKEVDEKP
ASTPWGSKMPFGQLMSEFGGSGTGGWVHGVSFSASGSRLAWVSHDSTVSVADASKSVQVS
TLKTEFLPLLSVSFVSENSVVAAGHDCCPMLFNYDDRGCLTFVSKLDIPKQSIQRNMSAM
ERFRNMDKRATTEDRNTALETLHQNSITQVSIYEVDKQDCRKFCTTGIDGAMTIWDFKTL
ESSIQGLRIM

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ARPC1A

Symbol Nomenclature :

ARPC1A

SYNONYM :

Arc40; HEL-68; SOP2Hs; SOP2L

Designation :

SOP2-like protein|actin binding protein (Schizosaccharomyces pombe sop2-like)|actin-related protein 2/3 complex subunit 1A|epididymis luminal protein 68

Full Nomenclature :

actin related protein 2/3 complex, subunit 1A, 41kDa

GENEID :

10552

GenBank Accession :

EU832206

HGNC :

703

MIM :

604220

Vega :

OTTHUMG00000154553

Target GenBank:

EU832206

Reference Sequence Alignment

  

HsCD00510409        ATGTCACTGCATCAGTTTTTACTAGAGCCAATCACCTGTCATGCCTGGAACAGGGATCGT
NM_001190996.1      ----------------------------------------ATGCCTGGAACAGGGATCGT
NM_006409.3         ATGTCACTGCATCAGTTTTTACTAGAGCCAATCACCTGTCATGCCTGGAACAGGGATCGT
                                                            ********************

HsCD00510409        ACTCAGATTGCCCTCAGTCCCAATAATCACGAAGTGCACATCTATAAGAAGAACGGGAGC
NM_001190996.1      ACTCATTG--CCCTCAGTCCCAATAATCACGAAGTGCACATCTATAAGAAGAACGGGAGC
NM_006409.3         ACTCAGATTGCCCTCAGTCCCAATAATCACGAAGTGCACATCTATAAGAAGAACGGGAGC
                    ***** :   **************************************************

HsCD00510409        CAGTGGGTGAAAGCTCATGAACTCAAGGAGCACAACGGACACATCACAGGTATTGACTGG
NM_001190996.1      CAGTGGGTGAAAGCTCATGAACTCAAGGAGCACAACGGACACATCACAGGTATTGACTGG
NM_006409.3         CAGTGGGTGAAAGCTCATGAACTCAAGGAGCACAACGGACACATCACAGGTATTGACTGG
                    ************************************************************

HsCD00510409        GCTCCCAAGAGCGACCGCATTGTCACTTGTGGGGCAGACCGCAATGCCTATGTCTGGAGT
NM_001190996.1      GCTCCCAAGAGCGACCGCATTGTCACTTGTGGGGCAGACCGCAATGCCTATGTCTGGAGT
NM_006409.3         GCTCCCAAGAGCGACCGCATTGTCACTTGTGGGGCAGACCGCAATGCCTATGTCTGGAGT
                    ************************************************************

HsCD00510409        CAGAAAGATGGTGTTTGGAAGCCAACCCTGGTGATCCTGAGAATTAATCGCGCAGCTACT
NM_001190996.1      CAGAAAGATGGTGTTTGGAAGCCAACCCTGGTGATCCTGAGAATTAATCGCGCAGCTACT
NM_006409.3         CAGAAAGATGGTGTTTGGAAGCCAACCCTGGTGATCCTGAGAATTAATCGCGCAGCTACT
                    ************************************************************

HsCD00510409        TTTGTGAAGTGGTCCCCCCTAGAGAACAAATTTGCTGTGGGAAGTGGAGCACGACTCATT
NM_001190996.1      TTTGTGAAGTGGTCCCCCCTAGAGAACAAATTTGCTGTGGGAAGTGGAGCACGACTCATT
NM_006409.3         TTTGTGAAGTGGTCCCCCCTAGAGAACAAATTTGCTGTGGGAAGTGGAGCACGACTCATT
                    ************************************************************

HsCD00510409        TCTGTTTGTTACTTTGAGTCTGAAAATGACTGGTGGGTGAGCAAGCACATTAAAAAGCCG
NM_001190996.1      TCTGTTTGTTACTTTGAGTCTGAAAATGACTGGTGGGTGAGCAAGCACATTAAAAAGCCG
NM_006409.3         TCTGTTTGTTACTTTGAGTCTGAAAATGACTGGTGGGTGAGCAAGCACATTAAAAAGCCG
                    ************************************************************

HsCD00510409        ATTCGCTCCACAGTCCTCAGCTTGGATTGGCATCCCAACAACGTTTTGCTGGCAGCAGGA
NM_001190996.1      ATTCGCTCCACAGTCCTCAGCTTGGATTGGCATCCCAACAACGTTTTGCTGGCAGCAGGA
NM_006409.3         ATTCGCTCCACAGTCCTCAGCTTGGATTGGCATCCCAACAACGTTTTGCTGGCAGCAGGA
                    ************************************************************

HsCD00510409        TCATGTGACTTCAAATGCAGAGTGTTTTCTGCCTACATTAAAGAAGTGGATGAAAAGCCA
NM_001190996.1      TCATGTGACTTCAAATGCAGAGTGTTTTCTGCCTACATTAAAGAAGTGGATGAAAAGCCA
NM_006409.3         TCATGTGACTTCAAATGCAGAGTGTTTTCTGCCTACATTAAAGAAGTGGATGAAAAGCCA
                    ************************************************************

HsCD00510409        GCCAGCACGCCCTGGGGCAGCAAGATGCCTTTTGGGCAGCTGATGTCAGAGTTTGGTGGC
NM_001190996.1      GCCAGCACGCCCTGGGGCAGCAAGATGCCTTTTGGGCAGCTGATGTCAGAGTTTGGTGGC
NM_006409.3         GCCAGCACGCCCTGGGGCAGCAAGATGCCTTTTGGGCAGCTGATGTCAGAGTTTGGTGGC
                    ************************************************************

HsCD00510409        AGTGGCACTGGTGGCTGGGTCCACGGGGTAAGCTTCTCTGCCAGTGGGAGCCGCCTGGCC
NM_001190996.1      AGTGGCACTGGTGGCTGGGTCCACGGGGTAAGCTTCTCTGCCAGTGGGAGCCGCCTGGCC
NM_006409.3         AGTGGCACTGGTGGCTGGGTCCACGGGGTAAGCTTCTCTGCCAGTGGGAGCCGCCTGGCC
                    ************************************************************

HsCD00510409        TGGGTCAGCCACGACAGCACCGTGTCTGTTGCTGATGCCTCAAAAAGTGTGCAGGTCTCG
NM_001190996.1      TGGGTCAGCCACGACAGCACCGTGTCTGTTGCTGATGCCTCAAAAAGTGTGCAGGTCTCG
NM_006409.3         TGGGTCAGCCACGACAGCACCGTGTCTGTTGCTGATGCCTCAAAAAGTGTGCAGGTCTCG
                    ************************************************************

HsCD00510409        ACTCTGAAGACAGAGTTCCTGCCGCTCCTAAGTGTGTCATTTGTCTCAGAGAACAGCGTC
NM_001190996.1      ACTCTGAAGACAGAGTTCCTGCCGCTCCTAAGTGTGTCATTTGTCTCAGAGAACAGCGTC
NM_006409.3         ACTCTGAAGACAGAGTTCCTGCCGCTCCTAAGTGTGTCATTTGTCTCAGAGAACAGCGTC
                    ************************************************************

HsCD00510409        GTGGCTGCTGGCCATGACTGCTGCCCAATGCTCTTTAACTACGATGACCGCGGCTGCCTG
NM_001190996.1      GTGGCTGCTGGCCATGACTGCTGCCCAATGCTCTTTAACTACGATGACCGCGGCTGCCTG
NM_006409.3         GTGGCTGCTGGCCATGACTGCTGCCCAATGCTCTTTAACTACGATGACCGCGGCTGCCTG
                    ************************************************************

HsCD00510409        ACCTTCGTCTCCAAGTTAGATATTCCAAAACAGAGCATCCAACGCAACATGTCTGCCATG
NM_001190996.1      ACCTTCGTCTCCAAGTTAGATATTCCAAAACAGAGCATCCAACGCAACATGTCTGCCATG
NM_006409.3         ACCTTCGTCTCCAAGTTAGATATTCCAAAACAGAGCATCCAACGCAACATGTCTGCCATG
                    ************************************************************

HsCD00510409        GAACGCTTCCGCAACATGGACAAGAGAGCCACAACTGAGGACCGCAACACGGCCTTGGAG
NM_001190996.1      GAACGCTTCCGCAACATGGACAAGAGAGCCACAACTGAGGACCGCAACACGGCCTTGGAG
NM_006409.3         GAACGCTTCCGCAACATGGACAAGAGAGCCACAACTGAGGACCGCAACACGGCCTTGGAG
                    ************************************************************

HsCD00510409        ACGCTGCACCAGAATAGCATCACTCAAGTCTCTATTTATGAGGTGGACAAGCAAGATTGT
NM_001190996.1      ACGCTGCACCAGAATAGCATCACTCAAGTCTCTATTTATGAGGTGGACAAGCAAGATTGT
NM_006409.3         ACGCTGCACCAGAATAGCATCACTCAAGTCTCTATTTATGAGGTGGACAAGCAAGATTGT
                    ************************************************************

HsCD00510409        CGCAAATTTTGCACTACTGGCATCGATGGAGCCATGACAATTTGGGATTTCAAGACCCTC
NM_001190996.1      CGCAAATTTTGCACTACTGGCATCGATGGAGCCATGACAATTTGGGATTTCAAGACCCTC
NM_006409.3         CGCAAATTTTGCACTACTGGCATCGATGGAGCCATGACAATTTGGGATTTCAAGACCCTC
                    ************************************************************

HsCD00510409        GAGTCTTCCATCCAGGGCCTCCGGATAATG---
NM_001190996.1      GAGTCTTCCATCCAGGGCCTCCGGATAATGTGA
NM_006409.3         GAGTCTTCCATCCAGGGCCTCCGGATAATGTGA
                    ******************************

Pathways

Panther :

Cytoskeletal regulation by Rho GTPase

Panther :

Huntington disease

Panther :

Inflammation mediated by chemokine and cytokine signaling pathway

Panther :

Integrin signalling pathway

Reactome :

Fcgamma receptor (FCGR) dependent phagocytosis

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

Regulation of actin dynamics for phagocytic cup formation

Protein

SMART domain :

WD40 : WD40 repeats

HPRD :

07252

Clone

Cloning Information :

609592

Original Clone ID :

ccsbBroadEn_02466

Organism

Species Specific ID:

10552

Genome

Chromosome :

Map Location :

7q22.1

Ensembl :

ENSG00000241685

Reagents

Labome :

ARPC1A-antibody