DNASU Plasmid | CCBP2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ACKR2

Gene Name: atypical chemokine receptor 2

Original Clone ID:

ccsbBroadEn_00334

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC008816.2,BC011631.2,BC020558.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00510950

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1153nts

Open reading frame: 1 to 1153

Reference Sequence Alignment

ATGGCCGCCACTGCCTCTCCGCAGCCACTCGCCACTGAGGATGCCGATTCTGAGAATAGC
AGCTTCTATTACTATGACTACCTGGATGAAGTGGCCTTCATGCTCTGCAGGAAGGATGCA
GTGGTGTCCTTTGGCAAAGTCTTCCTCCCAGTCTTCTATAGCCTGATTTTTGTGTTGGGC
CTCAGCGGGAACCTCCTTCTTCTCATGGTCTTGCTCCGTTACGTGCCTCGCAGGCGGATG
GTTGAGATCTATCTGCTGAATCTGGCCATCTCCAACCTTCTGTTTCTGGTGACACTGCCC
TTCTGGGGCATCTCCGTGGCCTGGCATTGGGTCTTCGGGAGTTTCTTGTGCAAGATGGTG
AGCACTCTTTATACTATTAACTTTTACAGTGGCATCTTTTTCATTAGCTGCATGAGCCTG
GACAAGTACCTGGAGATCGTTCATGCTCAGCCCTACCACAGGCTGAGGACCCGGGCCAAG
AGCCTGCTCCTTGCTACCATAGTATGGGCTGTGTCCCTGGCCGTCTCCATCCCTGATATG
GTCTTTGTACAGACACATGAAAATCCCAAGGGTGTGTGGAACTGCCACGCAGATTTCGGC
GGGCATGGGACCATTTGGAAGCTCTTCCTCCGCTTCCAGCAGAACCTCCTAGGGTTTCTC
CTTCCACTCCTTGCCATGATCTTCTTCTACTCCCGTATTGGTTGTGTCTTGGTGAGGCTG
AGGCCCGCAGGCCAGGGCCGGGCTTTAAAAATAGCTGCAGCCTTGGTGGTGGCCTTCTTC
GTGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGACCTGCAAGTA
TTCGGGAACTGTGAGGTCAGCCAGCATCTAGACTACGCACTCCAGGTAACAGAGAGCATC
GCCTTCCTTCACTGCTGCTTTTCCCCCATCCTGTATGCCTTCTCCAGTCACCGCTTCCGC
CAGTACCTGAAGGCTTTCCTGGCTGCCGTGCTTGGATGGCACCTGGCACCTGGCACTGCC
CAGGCCTCATTATCCAGCTGTTCTGAGAGCAGCATACTTACTGCCCAAGAGGAAATGACT
GGCATGAATGACCTTGGAGAGAGGCAGTCTGAGAACTACCCTAACAAGGAGGATGTGGGG
AATAAATCAGCC

Protein sequence predicted based on nucleotide sequence

MAATASPQPLATEDADSENSSFYYYDYLDEVAFMLCRKDAVVSFGKVFLPVFYSLIFVLG
LSGNLLLLMVLLRYVPRRRMVEIYLLNLAISNLLFLVTLPFWGISVAWHWVFGSFLCKMV
STLYTINFYSGIFFISCMSLDKYLEIVHAQPYHRLRTRAKSLLLATIVWAVSLAVSIPDM
VFVQTHENPKGVWNCHADFGGHGTIWKLFLRFQQNLLGFLLPLLAMIFFYSRIGCVLVRL
RPAGQGRALKIAAALVVAFFVLWFPYNLTLFLHTLLDLQVFGNCEVSQHLDYALQVTESI
AFLHCCFSPILYAFSSHRFRQYLKAFLAAVLGWHLAPGTAQASLSSCSESSILTAQEEMT
GMNDLGERQSENYPNKEDVGNKSA

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ACKR2

Symbol Nomenclature :

ACKR2

SYNONYM :

CCBP2; CCR10; CCR9; CMKBR9; D6; hD6

Designation :

Full Nomenclature :

atypical chemokine receptor 2

GENEID :

1238

GenBank Accession :

EU832737

HGNC :

1565

MIM :

602648

Vega :

OTTHUMG00000186377

Target GenBank:

EU832737

Reference Sequence Alignment

  

HsCD00510950      ATGGCCGCCACTGCCTCTCCGCAGCCACTCGCCACTGAGGATGCCGATTCTGAGAATAGC
NM_001296.4       ATGGCCGCCACTGCCTCTCCGCAGCCACTCGCCACTGAGGATGCCGATTCTGAGAATAGC
                  ************************************************************

HsCD00510950      AGCTTCTATTACTATGACTACCTGGATGAAGTGGCCTTCATGCTCTGCAGGAAGGATGCA
NM_001296.4       AGCTTCTATTACTATGACTACCTGGATGAAGTGGCCTTCATGCTCTGCAGGAAGGATGCA
                  ************************************************************

HsCD00510950      GTGGTGTCCTTTGGCAAAGTCTTCCTCCCAGTCTTCTATAGCCTGATTTTTGTGTTGGGC
NM_001296.4       GTGGTGTCCTTTGGCAAAGTCTTCCTCCCAGTCTTCTATAGCCTGATTTTTGTGTTGGGC
                  ************************************************************

HsCD00510950      CTCAGCGGGAACCTCCTTCTTCTCATGGTCTTGCTCCGTTACGTGCCTCGCAGGCGGATG
NM_001296.4       CTCAGCGGGAACCTCCTTCTTCTCATGGTCTTGCTCCGTTACGTGCCTCGCAGGCGGATG
                  ************************************************************

HsCD00510950      GTTGAGATCTATCTGCTGAATCTGGCCATCTCCAACCTTCTGTTTCTGGTGACACTGCCC
NM_001296.4       GTTGAGATCTATCTGCTGAATCTGGCCATCTCCAACCTTCTGTTTCTGGTGACACTGCCC
                  ************************************************************

HsCD00510950      TTCTGGGGCATCTCCGTGGCCTGGCATTGGGTCTTCGGGAGTTTCTTGTGCAAGATGGTG
NM_001296.4       TTCTGGGGCATCTCCGTGGCCTGGCATTGGGTCTTCGGGAGTTTCTTGTGCAAGATGGTG
                  ************************************************************

HsCD00510950      AGCACTCTTTATACTATTAACTTTTACAGTGGCATCTTTTTCATTAGCTGCATGAGCCTG
NM_001296.4       AGCACTCTTTATACTATTAACTTTTACAGTGGCATCTTTTTCATTAGCTGCATGAGCCTG
                  ************************************************************

HsCD00510950      GACAAGTACCTGGAGATCGTTCATGCTCAGCCCTACCACAGGCTGAGGACCCGGGCCAAG
NM_001296.4       GACAAGTACCTGGAGATCGTTCATGCTCAGCCCTACCACAGGCTGAGGACCCGGGCCAAG
                  ************************************************************

HsCD00510950      AGCCTGCTCCTTGCTACCATAGTATGGGCTGTGTCCCTGGCCGTCTCCATCCCTGATATG
NM_001296.4       AGCCTGCTCCTTGCTACCATAGTATGGGCTGTGTCCCTGGCCGTCTCCATCCCTGATATG
                  ************************************************************

HsCD00510950      GTCTTTGTACAGACACATGAAAATCCCAAGGGTGTGTGGAACTGCCACGCAGATTTCGGC
NM_001296.4       GTCTTTGTACAGACACATGAAAATCCCAAGGGTGTGTGGAACTGCCACGCAGATTTCGGC
                  ************************************************************

HsCD00510950      GGGCATGGGACCATTTGGAAGCTCTTCCTCCGCTTCCAGCAGAACCTCCTAGGGTTTCTC
NM_001296.4       GGGCATGGGACCATTTGGAAGCTCTTCCTCCGCTTCCAGCAGAACCTCCTAGGGTTTCTC
                  ************************************************************

HsCD00510950      CTTCCACTCCTTGCCATGATCTTCTTCTACTCCCGTATTGGTTGTGTCTTGGTGAGGCTG
NM_001296.4       CTTCCACTCCTTGCCATGATCTTCTTCTACTCCCGTATTGGTTGTGTCTTGGTGAGGCTG
                  ************************************************************

HsCD00510950      AGGCCCGCAGGCCAGGGCCGGGCTTTAAAAATAGCTGCAGCCTTGGTGGTGGCCTTCTTC
NM_001296.4       AGGCCCGCAGGCCAGGGCCGGGCTTTAAAAATAGCTGCAGCCTTGGTGGTGGCCTTCTTC
                  ************************************************************

HsCD00510950      GTGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGACCTGCAAGTA
NM_001296.4       GTGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGACCTGCAAGTA
                  ************************************************************

HsCD00510950      TTCGGGAACTGTGAGGTCAGCCAGCATCTAGACTACGCACTCCAGGTAACAGAGAGCATC
NM_001296.4       TTCGGGAACTGTGAGGTCAGCCAGCATCTAGACTACGCACTCCAGGTAACAGAGAGCATC
                  ************************************************************

HsCD00510950      GCCTTCCTTCACTGCTGCTTTTCCCCCATCCTGTATGCCTTCTCCAGTCACCGCTTCCGC
NM_001296.4       GCCTTCCTTCACTGCTGCTTTTCCCCCATCCTGTATGCCTTCTCCAGTCACCGCTTCCGC
                  ************************************************************

HsCD00510950      CAGTACCTGAAGGCTTTCCTGGCTGCCGTGCTTGGATGGCACCTGGCACCTGGCACTGCC
NM_001296.4       CAGTACCTGAAGGCTTTCCTGGCTGCCGTGCTTGGATGGCACCTGGCACCTGGCACTGCC
                  ************************************************************

HsCD00510950      CAGGCCTCATTATCCAGCTGTTCTGAGAGCAGCATACTTACTGCCCAAGAGGAAATGACT
NM_001296.4       CAGGCCTCATTATCCAGCTGTTCTGAGAGCAGCATACTTACTGCCCAAGAGGAAATGACT
                  ************************************************************

HsCD00510950      GGCATGAATGACCTTGGAGAGAGGCAGTCTGAGAACTACCCTAACAAGGAGGATGTGGGG
NM_001296.4       GGCATGAATGACCTTGGAGAGAGGCAGTCTGAGAACTACCCTAACAAGGAGGATGTGGGG
                  ************************************************************

HsCD00510950      AATAAATCAGCC---
NM_001296.4       AATAAATCAGCCTGA
                  ************

Pathways

Reactome :

Chemokine receptors bind chemokines

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

GPCR ligand binding

Reactome :

Peptide ligand-binding receptors

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

WikiPathway :

GPCRs, Class A Rhodopsin-like

Protein

HPRD :

09095

Clone

Cloning Information :

610752

Original Clone ID :

ccsbBroadEn_00334

Organism

Species Specific ID:

1238

Genome

Chromosome :

Map Location :

3p21.3

Ensembl :

ENSG00000144648

Reagents

Labome :

CCBP2-antibody