DNASU Plasmid | RDBP (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: NELFE

Gene Name: negative elongation factor complex member E

Original Clone ID:

ccsbBroadEn_01845

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC025235.1,BC050617.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511021

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1141nts

Open reading frame: 1 to 1141

Reference Sequence Alignment

ATGTTGGTGATACCCCCCGGACTGAGCGAGGAAGAGGAGGCTCTGCAGAAGAAATTCAAC
AAGCTCAAGAAAAAGAAAAAGGCATTGCTGGCTCTGAAGAAGCAAAGTAGCAGCAGCACA
ACCAGCCAAGGTGGTGTCAAACGCTCACTATCAGAGCAGCCTGTCATGGACACAGCCACA
GCAACAGAGCAGGCAAAGCAGCTGGTGAAGTCAGGAGCCATCAGTGCCATCAAGGCTGAG
ACCAAGAACTCAGGCTTCAAGCGTTCTCGAACCCTTGAGGGGAAGTTAAAGGACCCCGAG
AAGGGACCAGTCCCCACTTTCCAGCCGTTCCAGAGGAGCATATCTGCTGATGATGACCTG
CAAGAGTCATCCAGACGTCCCCAGAGGAAATCTCTGTATGAGAGCTTTGTGTCTTCTAGT
GATCGACTTCGAGAACTAGGACCAGATGGAGAAGAGGCAGAGGGCCCAGGGGCTGGTGAT
GGTCCCCCTCGAAGCTTTGACTGGGGCTATGAAGAACGCAGTGGTGCCCACTCCTCAGCC
TCCCCTCCCCGAAGCCGCAGCCGGGACCGCAGCCATGAGAGGAACCGGGACAGAGACCGA
GATCGGGAGCGGGATCGAGACCGGGATCGAGACAGAGACAGAGAGCGGGACAGGGATCGG
GATCGGGATCGAGATCGAGACCGGGAACGGGACAGGGATCGGGAGCGGGATCGAGACCGA
GACCGAGAGGGTCCTTTCCGCAGGTCGGATTCATTCCCTGAACGGCGAGCCCCTAGGAAA
GGGAATACTCTCTATGTATATGGAGAAGACATGACACCCACCCTTCTCCGTGGGGCCTTC
TCTCCTTTTGGAAACATCATTGACCTCTCCATGGACCCACCCAGAAACTGTGCCTTCGTC
ACCTATGAAAAGATGGAGTCAGCAGATCAGGCCGTTGCTGAGCTCAACGGGACCCAGGTG
GAGTCTGTACAGCTCAAAGTCAACATAGCCCGAAAACAGCCCATGCTGGATGCCGCTACT
GGCAAGTCTGTCTGGGGCTCCCTCGCTGTCCAGAACAGCCCTAAGGGTTGCCACCGGGAC
AAGAGGACCCAGATTGTCTACAGTGATGACGTCTACAAGGAAAACCTTGTGGATGGCTTC

Protein sequence predicted based on nucleotide sequence

MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTAT
ATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDL
QESSRRPQRKSLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSA
SPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDR
DREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFV
TYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRD
KRTQIVYSDDVYKENLVDGF

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

NELFE

Symbol Nomenclature :

NELFE

SYNONYM :

D6S45; NELF-E; RD; RDBP; RDP

Designation :

Full Nomenclature :

negative elongation factor complex member E

GENEID :

7936

Locus Tag :

DADB-122G4.4

GenBank Accession :

DQ896847

HGNC :

13974

MIM :

154040

Vega :

OTTHUMG00000031046

Target GenBank:

DQ896847

Reference Sequence Alignment

  

HsCD00511021      ATGTTGGTGATACCCCCCGGACTGAGCGAGGAAGAGGAGGCTCTGCAGAAGAAATTCAAC
NM_002904.5       ATGTTGGTGATACCCCCCGGACTGAGCGAGGAAGAGGAGGCTCTGCAGAAGAAATTCAAC
                  ************************************************************

HsCD00511021      AAGCTCAAGAAAAAGAAAAAGGCATTGCTGGCTCTGAAGAAGCAAAGTAGCAGCAGCACA
NM_002904.5       AAGCTCAAGAAAAAGAAAAAGGCATTGCTGGCTCTGAAGAAGCAAAGTAGCAGCAGCACA
                  ************************************************************

HsCD00511021      ACCAGCCAAGGTGGTGTCAAACGCTCACTATCAGAGCAGCCTGTCATGGACACAGCCACA
NM_002904.5       ACCAGCCAAGGTGGTGTCAAACGCTCACTATCAGAGCAGCCTGTCATGGACACAGCCACA
                  ************************************************************

HsCD00511021      GCAACAGAGCAGGCAAAGCAGCTGGTGAAGTCAGGAGCCATCAGTGCCATCAAGGCTGAG
NM_002904.5       GCAACAGAGCAGGCAAAGCAGCTGGTGAAGTCAGGAGCCATCAGTGCCATCAAGGCTGAG
                  ************************************************************

HsCD00511021      ACCAAGAACTCAGGCTTCAAGCGTTCTCGAACCCTTGAGGGGAAGTTAAAGGACCCCGAG
NM_002904.5       ACCAAGAACTCAGGCTTCAAGCGTTCTCGAACCCTTGAGGGGAAGTTAAAGGACCCCGAG
                  ************************************************************

HsCD00511021      AAGGGACCAGTCCCCACTTTCCAGCCGTTCCAGAGGAGCATATCTGCTGATGATGACCTG
NM_002904.5       AAGGGACCAGTCCCCACTTTCCAGCCGTTCCAGAGGAGCATATCTGCTGATGATGACCTG
                  ************************************************************

HsCD00511021      CAAGAGTCATCCAGACGTCCCCAGAGGAAATCTCTGTATGAGAGCTTTGTGTCTTCTAGT
NM_002904.5       CAAGAGTCATCCAGACGTCCCCAGAGGAAATCTCTGTATGAGAGCTTTGTGTCTTCTAGT
                  ************************************************************

HsCD00511021      GATCGACTTCGAGAACTAGGACCAGATGGAGAAGAGGCAGAGGGCCCAGGGGCTGGTGAT
NM_002904.5       GATCGACTTCGAGAACTAGGACCAGATGGAGAAGAGGCAGAGGGCCCAGGGGCTGGTGAT
                  ************************************************************

HsCD00511021      GGTCCCCCTCGAAGCTTTGACTGGGGCTATGAAGAACGCAGTGGTGCCCACTCCTCAGCC
NM_002904.5       GGTCCCCCTCGAAGCTTTGACTGGGGCTATGAAGAACGCAGTGGTGCCCACTCCTCAGCC
                  ************************************************************

HsCD00511021      TCCCCTCCCCGAAGCCGCAGCCGGGACCGCAGCCATGAGAGGAACCGGGACAGAGACCGA
NM_002904.5       TCCCCTCCCCGAAGCCGCAGCCGGGACCGCAGCCATGAGAGGAACCGGGACAGAGACCGA
                  ************************************************************

HsCD00511021      GATCGGGAGCGGGATCGAGACCGGGATCGAGACAGAGACAGAGAGCGGGACAGGGATCGG
NM_002904.5       GATCGGGAGCGGGATCGAGACCGGGATCGAGACAGAGACAGAGAGCGGGACAGGGATCGG
                  ************************************************************

HsCD00511021      GATCGGGATCGAGATCGAGACCGGGAACGGGACAGGGATCGGGAGCGGGATCGAGACCGA
NM_002904.5       GATCGGGATCGAGATCGAGACCGGGAACGGGACAGGGATCGGGAGCGGGATCGAGACCGA
                  ************************************************************

HsCD00511021      GACCGAGAGGGTCCTTTCCGCAGGTCGGATTCATTCCCTGAACGGCGAGCCCCTAGGAAA
NM_002904.5       GACCGAGAGGGTCCTTTCCGCAGGTCGGATTCATTCCCTGAACGGCGAGCCCCTAGGAAA
                  ************************************************************

HsCD00511021      GGGAATACTCTCTATGTATATGGAGAAGACATGACACCCACCCTTCTCCGTGGGGCCTTC
NM_002904.5       GGGAATACTCTCTATGTATATGGAGAAGACATGACACCCACCCTTCTCCGTGGGGCCTTC
                  ************************************************************

HsCD00511021      TCTCCTTTTGGAAACATCATTGACCTCTCCATGGACCCACCCAGAAACTGTGCCTTCGTC
NM_002904.5       TCTCCTTTTGGAAACATCATTGACCTCTCCATGGACCCACCCAGAAACTGTGCCTTCGTC
                  ************************************************************

HsCD00511021      ACCTATGAAAAGATGGAGTCAGCAGATCAGGCCGTTGCTGAGCTCAACGGGACCCAGGTG
NM_002904.5       ACCTATGAAAAGATGGAGTCAGCAGATCAGGCCGTTGCTGAGCTCAACGGGACCCAGGTG
                  ************************************************************

HsCD00511021      GAGTCTGTACAGCTCAAAGTCAACATAGCCCGAAAACAGCCCATGCTGGATGCCGCTACT
NM_002904.5       GAGTCTGTACAGCTCAAAGTCAACATAGCCCGAAAACAGCCCATGCTGGATGCCGCTACT
                  ************************************************************

HsCD00511021      GGCAAGTCTGTCTGGGGCTCCCTCGCTGTCCAGAACAGCCCTAAGGGTTGCCACCGGGAC
NM_002904.5       GGCAAGTCTGTCTGGGGCTCCCTCGCTGTCCAGAACAGCCCTAAGGGTTGCCACCGGGAC
                  ************************************************************

HsCD00511021      AAGAGGACCCAGATTGTCTACAGTGATGACGTCTACAAGGAAAACCTTGTGGATGGCTTC
NM_002904.5       AAGAGGACCCAGATTGTCTACAGTGATGACGTCTACAAGGAAAACCTTGTGGATGGCTTC
                  ************************************************************

HsCD00511021      ---
NM_002904.5       TAG

Pathways

Reactome :

Abortive elongation of HIV-1 transcript in the absence of Tat

Reactome :

Disease

Reactome :

Elongation arrest and recovery

Reactome :

Formation of HIV elongation complex in the absence of HIV Tat

Reactome :

Formation of HIV-1 elongation complex containing HIV-1 Tat

Reactome :

Formation of RNA Pol II elongation complex

Reactome :

Formation of the Early Elongation Complex

Reactome :

Formation of the HIV-1 Early Elongation Complex

Reactome :

Gene Expression

Reactome :

HIV elongation arrest and recovery

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

HIV Transcription Elongation

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Pausing and recovery of HIV elongation

Reactome :

Pausing and recovery of Tat-mediated HIV elongation

Reactome :

RNA Polymerase II Pre-transcription Events

Reactome :

RNA Polymerase II Transcription

Reactome :

RNA Polymerase II Transcription Elongation

Reactome :

Tat-mediated elongation of the HIV-1 transcript

Reactome :

Tat-mediated HIV elongation arrest and recovery

Reactome :

Transcription

Reactome :

Transcription of the HIV genome

Protein

SMART domain :

RRM : RNA recognition motif

HPRD :

01096

Clone

Cloning Information :

610823

Original Clone ID :

ccsbBroadEn_01845

Organism

Species Specific ID:

7936

Genome

Chromosome :

Map Location :

6p21.3

Ensembl :

ENSG00000204356

Reagents

Labome :

NELFE-antibody