DNASU Plasmid | ZXDC (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZXDC

Gene Name: ZXD family zinc finger C

Original Clone ID:

ccsbBroadEn_12561

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC002940.2,BC012729.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511062

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1174nts

Open reading frame: 1 to 1174

Reference Sequence Alignment

ATGAAGGCGCACATGGTCAGACAGCACAGCCGGCGCCAAGATCTCTTACCTCAGCTAGAA
GCTCCGAGTTCTCTTACTCCCAGCAGTGAACTCAGCAGCCCAGGCCAAAGTGAGCTCACT
AACATGGATCTTGCTGCACTCTTCTCTGACACACCTGCCAATGCTAGTGGTTCTGCAGGT
GGGTCGGATGAGGCTCTGAACTCCGGAATCCTGACTATTGACGTCACTTCTGTGAGCTCC
TCTCTGGGAGGGAACCTCCCTGCTAATAATAGCTCCCTAGGGCCGATGGAACCCCTGGTC
CTGGTGGCCCACAGTGATATTCCCCCAAGCCTGGACAGCCCTCTGGTTCTCGGGACAGCA
GCCACGGTTCTGCAGCAGGGCAGCTTCAGTGTGGATGACGTGCAGACTGTGAGTGCAGGA
GCATTAGGCTGTCTGGTGGCTCTGCCCATGAAGAACTTGAGTGACGACCCACTGGCTTTG
ACCTCCAATAGTAACTTAGCAGCACATATCACCACACCGACCTCTTCGAGCACCCCCCGA
GAAAATGCCAGTGTCCCGGAACTGCTGGCTCCAATCAAGGTGGAGCCGGACTCGCCTTCT
CGCCCAGGAGCAGTTGGGCAGCAGGAAGGAAGCCATGGGCTGCCCCAGTCCACGTTGCCC
AGTCCAGCAGAGCAGCACGGTGCCCAGGACACAGAGCTCAGTGCAGGCACTGGCAACTTC
TATTTGGAAAGTGGGGGCTCAGCAAGAACTGATTACCGAGCCATTCAACTAGCCAAGGAA
AAAAAGCAGAGAGGAGCGGGGAGCAATGCAGGAGCCTCACAGTCTACTCAGAGAAAAATA
AAAGAAGGCAAAATGAGTCCTCCCCATTTCCATGCAAGCCAGAACAGTTGGTTGTGTGGG
AGCCTCGTGGTGCCCAGCGGAGGACGGCCAGGACCAGCTCCAGCAGCTGGGGTGCAGTGC
GGGGCGCAGGGCGTCCAGGTCCAGCTGGTGCAGGATGACCCCTCCGGCGAAGGTGTCCTG
CCCTCGGCCCGCGGCCCAGCCACCTTCCTCCCCTTCCTCACTGTGGACCTGCCCGTCTAC
GTCCTCCAGGAGGTGCTCCCCTCATCTGGAGGCCCTGCTGGACCGGAGGCCACCCAGTTC
CCAGGAAGCACTATCAACCTGCAGGATCTGCAG

Protein sequence predicted based on nucleotide sequence

MKAHMVRQHSRRQDLLPQLEAPSSLTPSSELSSPGQSELTNMDLAALFSDTPANASGSAG
GSDEALNSGILTIDVTSVSSSLGGNLPANNSSLGPMEPLVLVAHSDIPPSLDSPLVLGTA
ATVLQQGSFSVDDVQTVSAGALGCLVALPMKNLSDDPLALTSNSNLAAHITTPTSSSTPR
ENASVPELLAPIKVEPDSPSRPGAVGQQEGSHGLPQSTLPSPAEQHGAQDTELSAGTGNF
YLESGGSARTDYRAIQLAKEKKQRGAGSNAGASQSTQRKIKEGKMSPPHFHASQNSWLCG
SLVVPSGGRPGPAPAAGVQCGAQGVQVQLVQDDPSGEGVLPSARGPATFLPFLTVDLPVY
VLQEVLPSSGGPAGPEATQFPGSTINLQDLQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

ZXDC

Symbol Nomenclature :

ZXDC

SYNONYM :

ZXDL

Designation :

SERH2790|ZXD-like zinc finger protein|zinc finger protein ZXDC

Full Nomenclature :

ZXD family zinc finger C

GENEID :

79364

Locus Tag :

UNQ2790

GenBank Accession :

EU446527

HGNC :

28160

Vega :

OTTHUMG00000162754

Target GenBank:

EU446527

Reference Sequence Alignment

  

HsCD00511062      ------------------------------------------------------------
NM_025112.4       ATGGACCTCCCGGCGCTGCTCCCCGCCCCGACTGCGCGCGGAGGGCAACATGGCGGCGGC
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       CCCGGCCCGCTCCGCCGAGCCCCAGCGCCGCTCGGCGCGAGCCCCGCGCGCCGCCGCCTG
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       CTACTGGTGCGGGGCCCTGAAGATGGCGGGCCCGGGGCGCGGCCCGGGGAGGCCTCCGGG
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       CCAAGCCCGCCGCCCGCCGAGGACGACAGCGACGGCGACTCTTTCTTGGTGCTGCTGGAA
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       GTGCCGCACGGCGGCGCTGCCGCCGAGGCTGCCGGATCACAGGAGGCCGAGCCTGGCTCC
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       CGTGTCAACCTGGCGAGCCGCCCCGAGCAGGGCCCCAGCGGCCCGGCCGCCCCCCCCGGC
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       CCTGGCGTAGCCCCGGCGGGCGCCGTCACCATCAGCAGCCAGGACCTGCTGGTGCGTCTC
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       GACCGCGGCGTCCTCGCGCTGTCTGCGCCGCCCGGCCCCGCAACCGCGGGCGCCGCCGCT
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       CCCCGCCGCGCGCCCCAGGCCTCCGGCCCCAGCACGCCCGGCTACCGCTGCCCCGAGCCG
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       CAGTGCGCGCTGGCCTTCGCCAAGAAGCACCAGCTCAAGGTGCACCTGCTCACGCACGGC
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       GGCGGTCAGGGCCGGCGGCCCTTCAAGTGCCCACTGGAGGGCTGTGGTTGGGCCTTCACA
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       ACGTCCTACAAGCTCAAGCGGCACCTGCAGTCGCACGACAAGCTGCGGCCCTTCGGCTGT
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       CCAGTGGGCGGCTGTGGCAAGAAGTTCACTACGGTCTATAACCTCAAGGCGCACATGAAG
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       GGCCACGAGCAGGAGAGCCTGTTCAAGTGCGAGGTGTGCGCCGAGCGCTTCCCCACGCAC
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       GCCAAGCTCAGCTCCCACCAGCGCAGCCACTTCGAGCCCGAGCGCCCTTACAAGTGTGAC
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       TTTCCCGGCTGTGAGAAGACATTTATCACAGTGAGTGCCCTGTTTTCCCATAACCGAGCC
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       CACTTCAGGGAACAAGAGCTCTTTTCCTGCTCCTTTCCTGGGTGCAGCAAGCAGTATGAT
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       AAAGCCTGTCGGCTGAAAATTCACCTGCGGAGCCATACAGGTGAAAGACCATTTATTTGT
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       GACTCTGACAGCTGTGGCTGGACCTTCACCAGCATGTCCAAACTTCTAAGGCACAGAAGG
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       AAACATGACGATGACCGGAGGTTTACCTGCCCTGTCGAGGGCTGTGGGAAATCATTCACC
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       AGAGCAGAGCATCTGAAAGGCCACAGCATAACCCACCTAGGCACAAAGCCGTTCGAGTGT
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       CCTGTGGAAGGATGTTGCGCGAGGTTCTCCGCTCGTAGCAGTCTGTACATTCACTCTAAG
                                                                              

HsCD00511062      ------------------------------------------------------------
NM_025112.4       AAACACGTGCAGGATGTGGGTGCTCCGAAAAGCCGTTGCCCAGTTTCTACCTGCAACAGA
                                                                              

HsCD00511062      ---------------------ATGAAGGCGCACATGGTCAGACAGCACAGCCGGCGCCAA
NM_025112.4       CTCTTCACCTCCAAGCACAGCATGAAGGCGCACATGGTCAGACAGCACAGCCGGCGCCAA
                                       ***************************************

HsCD00511062      GATCTCTTACCTCAGCTAGAAGCTCCGAGTTCTCTTACTCCCAGCAGTGAACTCAGCAGC
NM_025112.4       GATCTCTTACCTCAGCTAGAAGCTCCGAGTTCTCTTACTCCCAGCAGTGAACTCAGCAGC
                  ************************************************************

HsCD00511062      CCAGGCCAAAGTGAGCTCACTAACATGGATCTTGCTGCACTCTTCTCTGACACACCTGCC
NM_025112.4       CCAGGCCAAAGTGAGCTCACTAACATGGATCTTGCTGCACTCTTCTCTGACACACCTGCC
                  ************************************************************

HsCD00511062      AATGCTAGTGGTTCTGCAGGTGGGTCGGATGAGGCTCTGAACTCCGGAATCCTGACTATT
NM_025112.4       AATGCTAGTGGTTCTGCAGGTGGGTCGGATGAGGCTCTGAACTCCGGAATCCTGACTATT
                  ************************************************************

HsCD00511062      GACGTCACTTCTGTGAGCTCCTCTCTGGGAGGGAACCTCCCTGCTAATAATAGCTCCCTA
NM_025112.4       GACGTCACTTCTGTGAGCTCCTCTCTGGGAGGGAACCTCCCTGCTAATAATAGCTCCCTA
                  ************************************************************

HsCD00511062      GGGCCGATGGAACCCCTGGTCCTGGTGGCCCACAGTGATATTCCCCCAAGCCTGGACAGC
NM_025112.4       GGGCCGATGGAACCCCTGGTCCTGGTGGCCCACAGTGATATTCCCCCAAGCCTGGACAGC
                  ************************************************************

HsCD00511062      CCTCTGGTTCTCGGGACAGCAGCCACGGTTCTGCAGCAGGGCAGCTTCAGTGTGGATGAC
NM_025112.4       CCTCTGGTTCTCGGGACAGCAGCCACGGTTCTGCAGCAGGGCAGCTTCAGTGTGGATGAC
                  ************************************************************

HsCD00511062      GTGCAGACTGTGAGTGCAGGAGCATTAGGCTGTCTGGTGGCTCTGCCCATGAAGAACTTG
NM_025112.4       GTGCAGACTGTGAGTGCAGGAGCATTAGGCTGTCTGGTGGCTCTGCCCATGAAGAACTTG
                  ************************************************************

HsCD00511062      AGTGACGACCCACTGGCTTTGACCTCCAATAGTAACTTAGCAGCACATATCACCACACCG
NM_025112.4       AGTGACGACCCACTGGCTTTGACCTCCAATAGTAACTTAGCAGCACATATCACCACACCG
                  ************************************************************

HsCD00511062      ACCTCTTCGAGCACCCCCCGAGAAAATGCCAGTGTCCCGGAACTGCTGGCTCCAATCAAG
NM_025112.4       ACCTCTTCGAGCACCCCCCGAGAAAATGCCAGTGTCCCGGAACTGCTGGCTCCAATCAAG
                  ************************************************************

HsCD00511062      GTGGAGCCGGACTCGCCTTCTCGCCCAGGAGCAGTTGGGCAGCAGGAAGGAAGCCATGGG
NM_025112.4       GTGGAGCCGGACTCGCCTTCTCGCCCAGGAGCAGTTGGGCAGCAGGAAGGAAGCCATGGG
                  ************************************************************

HsCD00511062      CTGCCCCAGTCCACGTTGCCCAGTCCAGCAGAGCAGCACGGTGCCCAGGACACAGAGCTC
NM_025112.4       CTGCCCCAGTCCACGTTGCCCAGTCCAGCAGAGCAGCACGGTGCCCAGGACACAGAGCTC
                  ************************************************************

HsCD00511062      AGTGCAGGCACTGGCAACTTCTATTTGGAAAGTGGGGGCTCAGCAAGAACTGATTACCGA
NM_025112.4       AGTGCAGGCACTGGCAACTTCTATTTGGAAAGTGGGGGCTCAGCAAGAACTGATTACCGA
                  ************************************************************

HsCD00511062      GCCATTCAACTAGCCAAGGAAAAAAAGCAGAGAGGAGCGGGGAGCAATGCAGGAGCCTCA
NM_025112.4       GCCATTCAACTAGCCAAGGAAAAAAAGCAGAGAGGAGCGGGGAGCAATGCAGGAGCCTCA
                  ************************************************************

HsCD00511062      CAGTCTACTCAGAGAAAAATAAAAGAAGGCAAAATGAGTCCTCCCCATTTCCATGCAAGC
NM_025112.4       CAGTCTACTCAGAGAAAAATAAAAGAAGGCAAAATGAGTCCTCCCCATTTCCATGCAAGC
                  ************************************************************

HsCD00511062      CAGAACAGTTGGTTGTGTGGGAGCCTCGTGGTGCCCAGCGGAGGACGGCCAGGACCAGCT
NM_025112.4       CAGAACAGTTGGTTGTGTGGGAGCCTCGTGGTGCCCAGCGGAGGACGGCCAGGACCAGCT
                  ************************************************************

HsCD00511062      CCAGCAGCTGGGGTGCAGTGCGGGGCGCAGGGCGTCCAGGTCCAGCTGGTGCAGGATGAC
NM_025112.4       CCAGCAGCTGGGGTGCAGTGCGGGGCGCAGGGCGTCCAGGTCCAGCTGGTGCAGGATGAC
                  ************************************************************

HsCD00511062      CCCTCCGGCGAAGGTGTCCTGCCCTCGGCCCGCGGCCCAGCCACCTTCCTCCCCTTCCTC
NM_025112.4       CCCTCCGGCGAAGGTGTCCTGCCCTCGGCCCGCGGCCCAGCCACCTTCCTCCCCTTCCTC
                  ************************************************************

HsCD00511062      ACTGTGGACCTGCCCGTCTACGTCCTCCAGGAGGTGCTCCCCTCATCTGGAGGCCCTGCT
NM_025112.4       ACTGTGGACCTGCCCGTCTACGTCCTCCAGGAGGTGCTCCCCTCATCTGGAGGCCCTGCT
                  ************************************************************

HsCD00511062      GGACCGGAGGCCACCCAGTTCCCAGGAAGCACTATCAACCTGCAGGATCTGCAG---
NM_025112.4       GGACCGGAGGCCACCCAGTTCCCAGGAAGCACTATCAACCTGCAGGATCTGCAGTGA
                  ******************************************************