DNASU Plasmid | UBA5 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: UBA5

Gene Name: ubiquitin-like modifier activating enzyme 5

Original Clone ID:

ccsbBroadEn_04142

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC009737.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511276

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1213nts

Open reading frame: 1 to 1213

Reference Sequence Alignment

ATGGCGGAGTCTGTGGAGCGCCTGCAGCAGCGGGTCCAGGAGCTGGAGCGGGAACTTGCC
CAGGAGAGGAGTCTGCAGGTCCCGAGGAGCGGCGACGGAGGGGGCGGCCGGGTCCGCATC
GAGAAGATGAGCTCAGAGGTGGTGGATTCGAATCCCTACAGCCGCTTGATGGCATTGAAA
CGAATGGGAATTGTAAGCGACTATGAGAAAATCCGTACCTTTGCCGTAGCAATAGTAGGT
GTTGGTGGAGTAGGTAGTGTGACTGCTGAAATGCTGACAAGATGTGGCATTGGTAAGTTG
CTACTCTTTGATTATGACAAGGTGGAACTAGCCAATATGAATAGACTTTTCTTCCAACCT
CATCAAGCAGGATTAAGTAAAGTTCAAGCAGCAGAACATACTCTGAGGAACATTAATCCT
GATGTTCTTTTTGAAGTACACAACTATAATATAACCACAGTGGAAAACTTTCAACATTTC
ATGGATAGAATAAGTAATGGTGGGTTAGAAGAAGGAAAACCTGTTGATCTAGTTCTTAGC
TGTGTGGACAATTTTGAAGCTCGAATGACAATAAATACAGCTTGTAATGAACTTGGACAA
ACATGGATGGAATCTGGGGTCAGTGAAAATGCAGTTTCAGGGCATATACAGCTTATAATT
CCTGGAGAATCTGCTTGTTTTGCGTGTGCTCCACCACTTGTAGTTGCTGCAAATATTGAT
GAAAAGACTCTGAAACGAGAAGGTGTTTGTGCAGCCAGTCTTCCTACCACTATGGGTGTG
GTTGCTGGGATCTTAGTACAAAACGTGTTAAAGTTTCTGTTAAATTTTGGTACTGTTAGT
TTTTACCTTGGATACAATGCAATGCAGGATTTTTTTCCTACTATGTCCATGAAGCCAAAT
CCTCAGTGTGATGACAGAAATTGCAGGAAGCAGCAGGAGGAATATAAGAAAAAGGTAGCA
GCACTGCCTAAACAAGAGGTTATACAAGAAGAGGAAGAGATAATCCATGAAGATAATGAA
TGGGGTATTGAGCTGGTATCTGAGGTTTCAGAAGAGGAACTGAAAAATTTTTCAGGTCCA
GTTCCAGACTTACCTGAAGGAATTACAGTGGCATACACAATTCCAAAAAAGCAAGAAGAT
TCTGTCACTGAGTTAACAGTGGAAGATTCTGGTGAAAGCTTGGAAGACCTCATGGCCAAA
ATGAAGAATATG

Protein sequence predicted based on nucleotide sequence

MAESVERLQQRVQELERELAQERSLQVPRSGDGGGGRVRIEKMSSEVVDSNPYSRLMALK
RMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQP
HQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLS
CVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANID
EKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPN
PQCDDRNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGP
VPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Pathways

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

UBA5

Symbol Nomenclature :

UBA5

SYNONYM :

THIFP1; UBE1DC1

Designation :

UBA5, ubiquitin-activating enzyme E1 homolog|UFM1-activating enzyme|ubiquitin-activating enzyme 5|ubiquitin-activating enzyme E1 domain-containing protein 1|ubiquitin-activating enzyme E1-domain containing 1|ubiquitin-like modifier-activating enzyme 5

Full Nomenclature :

ubiquitin-like modifier activating enzyme 5

GENEID :

79876

GenBank Accession :

AM393124

HGNC :

23230

MIM :

610552

Target GenBank:

AM393124

Reference Sequence Alignment

  

HsCD00511276      ATGGCGGAGTCTGTGGAGCGCCTGCAGCAGCGGGTCCAGGAGCTGGAGCGGGAACTTGCC
NM_024818.3       ATGGCGGAGTCTGTGGAGCGCCTGCAGCAGCGGGTCCAGGAGCTGGAGCGGGAACTTGCC
                  ************************************************************

HsCD00511276      CAGGAGAGGAGTCTGCAGGTCCCGAGGAGCGGCGACGGAGGGGGCGGCCGGGTCCGCATC
NM_024818.3       CAGGAGAGGAGTCTGCAGGTCCCGAGGAGCGGCGACGGAGGGGGCGGCCGGGTCCGCATC
                  ************************************************************

HsCD00511276      GAGAAGATGAGCTCAGAGGTGGTGGATTCGAATCCCTACAGCCGCTTGATGGCATTGAAA
NM_024818.3       GAGAAGATGAGCTCAGAGGTGGTGGATTCGAATCCCTACAGCCGCTTGATGGCATTGAAA
                  ************************************************************

HsCD00511276      CGAATGGGAATTGTAAGCGACTATGAGAAAATCCGTACCTTTGCCGTAGCAATAGTAGGT
NM_024818.3       CGAATGGGAATTGTAAGCGACTATGAGAAAATCCGTACCTTTGCCGTAGCAATAGTAGGT
                  ************************************************************

HsCD00511276      GTTGGTGGAGTAGGTAGTGTGACTGCTGAAATGCTGACAAGATGTGGCATTGGTAAGTTG
NM_024818.3       GTTGGTGGAGTAGGTAGTGTGACTGCTGAAATGCTGACAAGATGTGGCATTGGTAAGTTG
                  ************************************************************

HsCD00511276      CTACTCTTTGATTATGACAAGGTGGAACTAGCCAATATGAATAGACTTTTCTTCCAACCT
NM_024818.3       CTACTCTTTGATTATGACAAGGTGGAACTAGCCAATATGAATAGACTTTTCTTCCAACCT
                  ************************************************************

HsCD00511276      CATCAAGCAGGATTAAGTAAAGTTCAAGCAGCAGAACATACTCTGAGGAACATTAATCCT
NM_024818.3       CATCAAGCAGGATTAAGTAAAGTTCAAGCAGCAGAACATACTCTGAGGAACATTAATCCT
                  ************************************************************

HsCD00511276      GATGTTCTTTTTGAAGTACACAACTATAATATAACCACAGTGGAAAACTTTCAACATTTC
NM_024818.3       GATGTTCTTTTTGAAGTACACAACTATAATATAACCACAGTGGAAAACTTTCAACATTTC
                  ************************************************************

HsCD00511276      ATGGATAGAATAAGTAATGGTGGGTTAGAAGAAGGAAAACCTGTTGATCTAGTTCTTAGC
NM_024818.3       ATGGATAGAATAAGTAATGGTGGGTTAGAAGAAGGAAAACCTGTTGATCTAGTTCTTAGC
                  ************************************************************

HsCD00511276      TGTGTGGACAATTTTGAAGCTCGAATGACAATAAATACAGCTTGTAATGAACTTGGACAA
NM_024818.3       TGTGTGGACAATTTTGAAGCTCGAATGACAATAAATACAGCTTGTAATGAACTTGGACAA
                  ************************************************************

HsCD00511276      ACATGGATGGAATCTGGGGTCAGTGAAAATGCAGTTTCAGGGCATATACAGCTTATAATT
NM_024818.3       ACATGGATGGAATCTGGGGTCAGTGAAAATGCAGTTTCAGGGCATATACAGCTTATAATT
                  ************************************************************

HsCD00511276      CCTGGAGAATCTGCTTGTTTTGCGTGTGCTCCACCACTTGTAGTTGCTGCAAATATTGAT
NM_024818.3       CCTGGAGAATCTGCTTGTTTTGCGTGTGCTCCACCACTTGTAGTTGCTGCAAATATTGAT
                  ************************************************************

HsCD00511276      GAAAAGACTCTGAAACGAGAAGGTGTTTGTGCAGCCAGTCTTCCTACCACTATGGGTGTG
NM_024818.3       GAAAAGACTCTGAAACGAGAAGGTGTTTGTGCAGCCAGTCTTCCTACCACTATGGGTGTG
                  ************************************************************

HsCD00511276      GTTGCTGGGATCTTAGTACAAAACGTGTTAAAGTTTCTGTTAAATTTTGGTACTGTTAGT
NM_024818.3       GTTGCTGGGATCTTAGTACAAAACGTGTTAAAGTTTCTGTTAAATTTTGGTACTGTTAGT
                  ************************************************************

HsCD00511276      TTTTACCTTGGATACAATGCAATGCAGGATTTTTTTCCTACTATGTCCATGAAGCCAAAT
NM_024818.3       TTTTACCTTGGATACAATGCAATGCAGGATTTTTTTCCTACTATGTCCATGAAGCCAAAT
                  ************************************************************

HsCD00511276      CCTCAGTGTGATGACAGAAATTGCAGGAAGCAGCAGGAGGAATATAAGAAAAAGGTAGCA
NM_024818.3       CCTCAGTGTGATGACAGAAATTGCAGGAAGCAGCAGGAGGAATATAAGAAAAAGGTAGCA
                  ************************************************************

HsCD00511276      GCACTGCCTAAACAAGAGGTTATACAAGAAGAGGAAGAGATAATCCATGAAGATAATGAA
NM_024818.3       GCACTGCCTAAACAAGAGGTTATACAAGAAGAGGAAGAGATAATCCATGAAGATAATGAA
                  ************************************************************

HsCD00511276      TGGGGTATTGAGCTGGTATCTGAGGTTTCAGAAGAGGAACTGAAAAATTTTTCAGGTCCA
NM_024818.3       TGGGGTATTGAGCTGGTATCTGAGGTTTCAGAAGAGGAACTGAAAAATTTTTCAGGTCCA
                  ************************************************************

HsCD00511276      GTTCCAGACTTACCTGAAGGAATTACAGTGGCATACACAATTCCAAAAAAGCAAGAAGAT
NM_024818.3       GTTCCAGACTTACCTGAAGGAATTACAGTGGCATACACAATTCCAAAAAAGCAAGAAGAT
                  ************************************************************

HsCD00511276      TCTGTCACTGAGTTAACAGTGGAAGATTCTGGTGAAAGCTTGGAAGACCTCATGGCCAAA
NM_024818.3       TCTGTCACTGAGTTAACAGTGGAAGATTCTGGTGAAAGCTTGGAAGACCTCATGGCCAAA
                  ************************************************************

HsCD00511276      ATGAAGAATATG---
NM_024818.3       ATGAAGAATATGTAG
                  ************

Pathways

Reactome :

Adaptive Immune System

Reactome :

Antigen processing: Ubiquitination & Proteasome degradation

Reactome :

Class I MHC mediated antigen processing & presentation

Reactome :

Immune System

Protein

HPRD :

15598

PDBe Mol* Structure Viewer

Clone

Cloning Information :

611336

Original Clone ID :

ccsbBroadEn_04142

Organism

Species Specific ID:

79876

Genome

Chromosome :

Map Location :

3q22.1

Ensembl :

ENSG00000081307

Reagents

Labome :

UBA5-antibody