DNASU Plasmid | BRF2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: BRF2

Gene Name: BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit

Original Clone ID:

ccsbBroadEn_03573

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC010648.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511399

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1258nts

Open reading frame: 1 to 1258

Reference Sequence Alignment

ATGCCAGGCAGAGGCCGCTGCCCGGACTGCGGCTCCACGGAGCTGGTGGAAGACTCGCAC
TATTCGCAGAGCCAGCTGGTGTGCTCCGACTGCGGCTGCGTGGTCACCGAGGGGGTCCTT
ACCACTACCTTCAGCGACGAGGGCAATCTCCGAGAGGTAACATATTCCCGAAGCACAGGG
GAAAACGAACAAGTTAGTCGCAGCCAGCAACGAGGTCTCCGGCGAGTGAGAGACCTTTGT
CGAGTTCTGCAGTTGCCACCAACATTTGAGGATACCGCGGTTGCCTACTACCAACAGGCA
TATCGGCACTCTGGCATCCGAGCGGCCAGGCTGCAAAAGAAGGAGGTGTTGGTTGGGTGC
TGCGTCTTAATCACCTGCCGACAGCATAACTGGCCCCTAACAATGGGGGCCATCTGCACG
CTGTTGTATGCAGATTTGGATGTGTTTTCTAGCACTTACATGCAGATAGTGAAGCTCCTG
GGACTGGATGTGCCATCTCTGTGCTTGGCAGAACTGGTGAAGACCTATTGCAGCAGCTTC
AAACTGTTCCAAGCTTCACCTTCTGTGCCAGCCAAATACGTGGAAGACAAAGAGAAGATG
CTGTCTCGAACAATGCAGTTGGTGGAGCTGGCAAATGAGACGTGGCTGGTGACCGGGAGG
CATCCCTTGCCCGTCATCACTGCTGCGACTTTCCTGGCTTGGCAGTCGCTGCAGCCTGCA
GATCGGCTTTCATGTTCCCTTGCCCGATTTTGTAAATTGGCAAATGTGGACCTGCCCTAC
CCGGCGTCCTCCCGCCTGCAGGAGCTGCTGGCTGTGCTGCTGCGGATGGCTGAGCAGCTG
GCCTGGTTACGAGTTCTGAGACTTGACAAACGGTCTGTGGTGAAGCACATCGGTGACCTT
CTCCAGCACCGCCAGTCACTGGTCCGCTCTGCCTTTCGGGATGGGACAGCAGAAGTGGAG
ACCCGAGAGAAGGAGCCACCGGGGTGGGGACAGGGGCAAGGAGAAGGGGAGGTGGGAAAT
AATTCCTTAGGTTTACCCCAGGGGAAGCGGCCGGCCAGTCCTGCCCTTCTCTTGCCACCC
TGCATGTTGAAGTCCCCGAAGCGGATCTGCCCTGTACCCCCTGTCTCCACTGTCACTGGA
GATGAGAACATTTCTGATAGTGAAATAGAACAGTATTTGCGTACCCCTCAGGAAGTTAGG
GACTTTCAGAGAGCCCAGGCTGCTAGACAGGCTGCCACGAGTGTCCCTAACCCTCCC

Protein sequence predicted based on nucleotide sequence

MPGRGRCPDCGSTELVEDSHYSQSQLVCSDCGCVVTEGVLTTTFSDEGNLREVTYSRSTG
ENEQVSRSQQRGLRRVRDLCRVLQLPPTFEDTAVAYYQQAYRHSGIRAARLQKKEVLVGC
CVLITCRQHNWPLTMGAICTLLYADLDVFSSTYMQIVKLLGLDVPSLCLAELVKTYCSSF
KLFQASPSVPAKYVEDKEKMLSRTMQLVELANETWLVTGRHPLPVITAATFLAWQSLQPA
DRLSCSLARFCKLANVDLPYPASSRLQELLAVLLRMAEQLAWLRVLRLDKRSVVKHIGDL
LQHRQSLVRSAFRDGTAEVETREKEPPGWGQGQGEGEVGNNSLGLPQGKRPASPALLLPP
CMLKSPKRICPVPPVSTVTGDENISDSEIEQYLRTPQEVRDFQRAQAARQAATSVPNPP

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

BRF2

Symbol Nomenclature :

BRF2

SYNONYM :

BRFU; TFIIIB50

Designation :

Full Nomenclature :

BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit

GENEID :

55290

Locus Tag :

PRO1470

GenBank Accession :

EU446514

HGNC :

17298

MIM :

607013

Target GenBank:

EU446514

Reference Sequence Alignment

  

HsCD00511399      ATGCCAGGCAGAGGCCGCTGCCCGGACTGCGGCTCCACGGAGCTGGTGGAAGACTCGCAC
NM_018310.3       ATGCCAGGCAGAGGCCGCTGCCCGGACTGCGGCTCCACGGAGCTGGTGGAAGACTCGCAC
                  ************************************************************

HsCD00511399      TATTCGCAGAGCCAGCTGGTGTGCTCCGACTGCGGCTGCGTGGTCACCGAGGGGGTCCTT
NM_018310.3       TATTCGCAGAGCCAGCTGGTGTGCTCCGACTGCGGCTGCGTGGTCACCGAGGGGGTCCTT
                  ************************************************************

HsCD00511399      ACCACTACCTTCAGCGACGAGGGCAATCTCCGAGAGGTAACATATTCCCGAAGCACAGGG
NM_018310.3       ACCACTACCTTCAGCGACGAGGGCAATCTCCGAGAGGTAACATATTCCCGAAGCACAGGG
                  ************************************************************

HsCD00511399      GAAAACGAACAAGTTAGTCGCAGCCAGCAACGAGGTCTCCGGCGAGTGAGAGACCTTTGT
NM_018310.3       GAAAACGAACAAGTTAGTCGCAGCCAGCAACGAGGTCTCCGGCGAGTGAGAGACCTTTGT
                  ************************************************************

HsCD00511399      CGAGTTCTGCAGTTGCCACCAACATTTGAGGATACCGCGGTTGCCTACTACCAACAGGCA
NM_018310.3       CGAGTTCTGCAGTTGCCACCAACATTTGAGGATACCGCGGTTGCCTACTACCAACAGGCA
                  ************************************************************

HsCD00511399      TATCGGCACTCTGGCATCCGAGCGGCCAGGCTGCAAAAGAAGGAGGTGTTGGTTGGGTGC
NM_018310.3       TATCGGCACTCTGGCATCCGAGCGGCCAGGCTGCAAAAGAAGGAGGTGTTGGTTGGGTGC
                  ************************************************************

HsCD00511399      TGCGTCTTAATCACCTGCCGACAGCATAACTGGCCCCTAACAATGGGGGCCATCTGCACG
NM_018310.3       TGCGTCTTAATCACCTGCCGACAGCATAACTGGCCCCTAACAATGGGGGCCATCTGCACG
                  ************************************************************

HsCD00511399      CTGTTGTATGCAGATTTGGATGTGTTTTCTAGCACTTACATGCAGATAGTGAAGCTCCTG
NM_018310.3       CTGTTGTATGCAGATTTGGATGTGTTTTCTAGCACTTACATGCAGATAGTGAAGCTCCTG
                  ************************************************************

HsCD00511399      GGACTGGATGTGCCATCTCTGTGCTTGGCAGAACTGGTGAAGACCTATTGCAGCAGCTTC
NM_018310.3       GGACTGGATGTGCCATCTCTGTGCTTGGCAGAACTGGTGAAGACCTATTGCAGCAGCTTC
                  ************************************************************

HsCD00511399      AAACTGTTCCAAGCTTCACCTTCTGTGCCAGCCAAATACGTGGAAGACAAAGAGAAGATG
NM_018310.3       AAACTGTTCCAAGCTTCACCTTCTGTGCCAGCCAAATACGTGGAAGACAAAGAGAAGATG
                  ************************************************************

HsCD00511399      CTGTCTCGAACAATGCAGTTGGTGGAGCTGGCAAATGAGACGTGGCTGGTGACCGGGAGG
NM_018310.3       CTGTCTCGAACAATGCAGTTGGTGGAGCTGGCAAATGAGACGTGGCTGGTGACCGGGAGG
                  ************************************************************

HsCD00511399      CATCCCTTGCCCGTCATCACTGCTGCGACTTTCCTGGCTTGGCAGTCGCTGCAGCCTGCA
NM_018310.3       CATCCCTTGCCCGTCATCACTGCTGCGACTTTCCTGGCTTGGCAGTCGCTGCAGCCTGCA
                  ************************************************************

HsCD00511399      GATCGGCTTTCATGTTCCCTTGCCCGATTTTGTAAATTGGCAAATGTGGACCTGCCCTAC
NM_018310.3       GATCGGCTTTCATGTTCCCTTGCCCGATTTTGTAAATTGGCAAATGTGGACCTGCCCTAC
                  ************************************************************

HsCD00511399      CCGGCGTCCTCCCGCCTGCAGGAGCTGCTGGCTGTGCTGCTGCGGATGGCTGAGCAGCTG
NM_018310.3       CCGGCGTCCTCCCGCCTGCAGGAGCTGCTGGCTGTGCTGCTGCGGATGGCTGAGCAGCTG
                  ************************************************************

HsCD00511399      GCCTGGTTACGAGTTCTGAGACTTGACAAACGGTCTGTGGTGAAGCACATCGGTGACCTT
NM_018310.3       GCCTGGTTACGAGTTCTGAGACTTGACAAACGGTCTGTGGTGAAGCACATCGGTGACCTT
                  ************************************************************

HsCD00511399      CTCCAGCACCGCCAGTCACTGGTCCGCTCTGCCTTTCGGGATGGGACAGCAGAAGTGGAG
NM_018310.3       CTCCAGCACCGCCAGTCACTGGTCCGCTCTGCCTTTCGGGATGGGACAGCAGAAGTGGAG
                  ************************************************************

HsCD00511399      ACCCGAGAGAAGGAGCCACCGGGGTGGGGACAGGGGCAAGGAGAAGGGGAGGTGGGAAAT
NM_018310.3       ACCCGAGAGAAGGAGCCACCGGGGTGGGGACAGGGGCAAGGAGAAGGGGAGGTGGGAAAT
                  ************************************************************

HsCD00511399      AATTCCTTAGGTTTACCCCAGGGGAAGCGGCCGGCCAGTCCTGCCCTTCTCTTGCCACCC
NM_018310.3       AATTCCTTAGGTTTACCCCAGGGGAAGCGGCCGGCCAGTCCTGCCCTTCTCTTGCCACCC
                  ************************************************************

HsCD00511399      TGCATGTTGAAGTCCCCGAAGCGGATCTGCCCTGTACCCCCTGTCTCCACTGTCACTGGA
NM_018310.3       TGCATGTTGAAGTCCCCGAAGCGGATCTGCCCTGTACCCCCTGTCTCCACTGTCACTGGA
                  ************************************************************

HsCD00511399      GATGAGAACATTTCTGATAGTGAAATAGAACAGTATTTGCGTACCCCTCAGGAAGTTAGG
NM_018310.3       GATGAGAACATTTCTGATAGTGAAATAGAACAGTATTTGCGTACCCCTCAGGAAGTTAGG
                  ************************************************************

HsCD00511399      GACTTTCAGAGAGCCCAGGCTGCTAGACAGGCTGCCACGAGTGTCCCTAACCCTCCC---
NM_018310.3       GACTTTCAGAGAGCCCAGGCTGCTAGACAGGCTGCCACGAGTGTCCCTAACCCTCCCTGA
                  *********************************************************

Pathways

Panther :

General transcription regulation

Reactome :

Gene Expression

Reactome :

RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription

Reactome :

RNA Polymerase III Abortive And Retractive Initiation

Reactome :

RNA Polymerase III Transcription

Reactome :

RNA Polymerase III Transcription Initiation

Reactome :

RNA Polymerase III Transcription Initiation From Type 3 Promoter

Reactome :

Transcription

Protein

HPRD :

06115

Clone

Cloning Information :

611553

Original Clone ID :

ccsbBroadEn_03573

Organism

Species Specific ID:

55290

Genome

Chromosome :

Map Location :

8p11.23

Reagents

Labome :

BRF2-antibody