DNASU Plasmid | MCHR1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: MCHR1

Gene Name: melanin-concentrating hormone receptor 1

Original Clone ID:

ccsbBroadEn_06315

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC001736.1,BC021146.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511466

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1267nts

Open reading frame: 1 to 1267

Reference Sequence Alignment

ATGTCAGTGGGAGCCATGAAGAAGGGAGTGGGGAGGGCAGTTGGGCTTGGAGGCGGCAGC
GGCTGCCAGGCTACGGAGGAAGACCCCCTTCCCGACTGCGGGGCTTGCGCTCCGGGACAA
GGTGGCAGGCGCTGGAGGCTGCCGCAGCCTGCGTGGGTGGAGGGGAGCTCAGCTCGGTTG
TGGGAGCAGGCGACCGGCACTGGCTGGATGGACCTGGAAGCCTCGCTGCTGCCCACTGGT
CCCAATGCCAGCAACACCTCTGATGGCCCCGATAACCTCACTTCGGCAGGATCACCTCCT
CGCACGGGGAGCATCTCCTACATCAACATCATCATGCCTTCGGTGTTCGGCACCATCTGC
CTCCTGGGCATCATCGGGAACTCCACGGTCATCTTCGCGGTCGTGAAGAAGTCCAAGCTG
CACTGGTGCAACAACGTCCCCGACATCTTCATCATCAACCTCTCGGTAGTAGATCTCCTC
TTTCTCCTGGGCATGCCCTTCATGATCCACCAGCTCATGGGCAATGGGGTGTGGCACTTT
GGGGAGACCATGTGCACCCTCATCACGGCCATGGATGCCAATAGTCAGTTCACCAGCACC
TACATCCTGACCGCCATGGCCATTGACCGCTACCTGGCCACTGTCCACCCCATCTCTTCC
ACGAAGTTCCGGAAGCCCTCTGTGGCCACCCTGGTGATCTGCCTCCTGTGGGCCCTCTCC
TTCATCAGCATCACCCCTGTGTGGCTGTATGCCAGACTCATCCCCTTCCCAGGAGGTGCA
GTGGGCTGCGGCATACGCCTGCCCAACCCAGACACTGACCTCTACTGGTTCACCCTGTAC
CAGTTTTTCCTGGCCTTTGCCCTGCCTTTTGTGGTCATCACAGCCGCATACGTGAGGATC
CTGCAGCGCATGACGTCCTCAGTGGCCCCCGCCTCCCAGCGCAGCATCCGGCTGCGGACA
AAGAGGGTGACCCGCACAGCCATCGCCATCTGTCTGGTCTTCTTTGTGTGCTGGGCACCC
TACTATGTGCTACAGCTGACCCAGTTGTCCATCAGCCGCCCGACCCTCACCTTTGTCTAC
TTATACAATGCGGCCATCAGCTTGGGCTATGCCAACAGCTGCCTCAACCCCTTTGTGTAC
ATCGTGCTCTGTGAGACGTTCCGCAAACGCTTGGTCCTGTCGGTGAAGCCTGCAGCCCAG
GGGCAGCTTCGCGCTGTCAGCAACGCTCAGACGGCTGACGAGGAGAGGACAGAAAGCAAA
GGCACC

Protein sequence predicted based on nucleotide sequence

MSVGAMKKGVGRAVGLGGGSGCQATEEDPLPDCGACAPGQGGRRWRLPQPAWVEGSSARL
WEQATGTGWMDLEASLLPTGPNASNTSDGPDNLTSAGSPPRTGSISYINIIMPSVFGTIC
LLGIIGNSTVIFAVVKKSKLHWCNNVPDIFIINLSVVDLLFLLGMPFMIHQLMGNGVWHF
GETMCTLITAMDANSQFTSTYILTAMAIDRYLATVHPISSTKFRKPSVATLVICLLWALS
FISITPVWLYARLIPFPGGAVGCGIRLPNPDTDLYWFTLYQFFLAFALPFVVITAAYVRI
LQRMTSSVAPASQRSIRLRTKRVTRTAIAICLVFFVCWAPYYVLQLTQLSISRPTLTFVY
LYNAAISLGYANSCLNPFVYIVLCETFRKRLVLSVKPAAQGQLRAVSNAQTADEERTESK
GT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

MCHR1

Symbol Nomenclature :

MCHR1

SYNONYM :

GPR24; MCH-1R; MCH1R; SLC-1; SLC1

Designation :

G protein-coupled receptor 24|MCH receptor 1|somatostatin receptor-like protein

Full Nomenclature :

melanin-concentrating hormone receptor 1

GENEID :

2847

Locus Tag :

CTA-229A8.4

GenBank Accession :

DQ895065

HGNC :

4479

MIM :

601751

Vega :

OTTHUMG00000150256

Target GenBank:

DQ895065

Reference Sequence Alignment

  

HsCD00511466      ATGTCAGTGGGAGCCATGAAGAAGGGAGTGGGGAGGGCAGTTGGGCTTGGAGGCGGCAGC
NM_005297.3       ATGTCAGTGGGAGCCATGAAGAAGGGAGTGGGGAGGGCAGTTGGGCTTGGAGGCGGCAGC
                  ************************************************************

HsCD00511466      GGCTGCCAGGCTACGGAGGAAGACCCCCTTCCCGACTGCGGGGCTTGCGCTCCGGGACAA
NM_005297.3       GGCTGCCAGGCTACGGAGGAAGACCCCCTTCCCAACTGCGGGGCTTGCGCTCCGGGACAA
                  *********************************.**************************

HsCD00511466      GGTGGCAGGCGCTGGAGGCTGCCGCAGCCTGCGTGGGTGGAGGGGAGCTCAGCTCGGTTG
NM_005297.3       GGTGGCAGGCGCTGGAGGCTGCCGCAGCCTGCGTGGGTGGAGGGGAGCTCAGCTCGGTTG
                  ************************************************************

HsCD00511466      TGGGAGCAGGCGACCGGCACTGGCTGGATGGACCTGGAAGCCTCGCTGCTGCCCACTGGT
NM_005297.3       TGGGAGCAGGCGACCGGCACTGGCTGGATGGACCTGGAAGCCTCGCTGCTGCCCACTGGT
                  ************************************************************

HsCD00511466      CCCAATGCCAGCAACACCTCTGATGGCCCCGATAACCTCACTTCGGCAGGATCACCTCCT
NM_005297.3       CCCAACGCCAGCAACACCTCTGATGGCCCCGATAACCTCACTTCGGCAGGATCACCTCCT
                  ***** ******************************************************

HsCD00511466      CGCACGGGGAGCATCTCCTACATCAACATCATCATGCCTTCGGTGTTCGGCACCATCTGC
NM_005297.3       CGCACGGGGAGCATCTCCTACATCAACATCATCATGCCTTCGGTGTTCGGCACCATCTGC
                  ************************************************************

HsCD00511466      CTCCTGGGCATCATCGGGAACTCCACGGTCATCTTCGCGGTCGTGAAGAAGTCCAAGCTG
NM_005297.3       CTCCTGGGCATCATCGGGAACTCCACGGTCATCTTCGCGGTCGTGAAGAAGTCCAAGCTG
                  ************************************************************

HsCD00511466      CACTGGTGCAACAACGTCCCCGACATCTTCATCATCAACCTCTCGGTAGTAGATCTCCTC
NM_005297.3       CACTGGTGCAACAACGTCCCCGACATCTTCATCATCAACCTCTCGGTAGTAGATCTCCTC
                  ************************************************************

HsCD00511466      TTTCTCCTGGGCATGCCCTTCATGATCCACCAGCTCATGGGCAATGGGGTGTGGCACTTT
NM_005297.3       TTTCTCCTGGGCATGCCCTTCATGATCCACCAGCTCATGGGCAATGGGGTGTGGCACTTT
                  ************************************************************

HsCD00511466      GGGGAGACCATGTGCACCCTCATCACGGCCATGGATGCCAATAGTCAGTTCACCAGCACC
NM_005297.3       GGGGAGACCATGTGCACCCTCATCACGGCCATGGATGCCAATAGTCAGTTCACCAGCACC
                  ************************************************************

HsCD00511466      TACATCCTGACCGCCATGGCCATTGACCGCTACCTGGCCACTGTCCACCCCATCTCTTCC
NM_005297.3       TACATCCTGACCGCCATGGCCATTGACCGCTACCTGGCCACTGTCCACCCCATCTCTTCC
                  ************************************************************

HsCD00511466      ACGAAGTTCCGGAAGCCCTCTGTGGCCACCCTGGTGATCTGCCTCCTGTGGGCCCTCTCC
NM_005297.3       ACGAAGTTCCGGAAGCCCTCTGTGGCCACCCTGGTGATCTGCCTCCTGTGGGCCCTCTCC
                  ************************************************************

HsCD00511466      TTCATCAGCATCACCCCTGTGTGGCTGTATGCCAGACTCATCCCCTTCCCAGGAGGTGCA
NM_005297.3       TTCATCAGCATCACCCCTGTGTGGCTGTATGCCAGACTCATCCCCTTCCCAGGAGGTGCA
                  ************************************************************

HsCD00511466      GTGGGCTGCGGCATACGCCTGCCCAACCCAGACACTGACCTCTACTGGTTCACCCTGTAC
NM_005297.3       GTGGGCTGCGGCATACGCCTGCCCAACCCAGACACTGACCTCTACTGGTTCACCCTGTAC
                  ************************************************************

HsCD00511466      CAGTTTTTCCTGGCCTTTGCCCTGCCTTTTGTGGTCATCACAGCCGCATACGTGAGGATC
NM_005297.3       CAGTTTTTCCTGGCCTTTGCCCTGCCTTTTGTGGTCATCACAGCCGCATACGTGAGGATC
                  ************************************************************

HsCD00511466      CTGCAGCGCATGACGTCCTCAGTGGCCCCCGCCTCCCAGCGCAGCATCCGGCTGCGGACA
NM_005297.3       CTGCAGCGCATGACGTCCTCAGTGGCCCCCGCCTCCCAGCGCAGCATCCGGCTGCGGACA
                  ************************************************************

HsCD00511466      AAGAGGGTGACCCGCACAGCCATCGCCATCTGTCTGGTCTTCTTTGTGTGCTGGGCACCC
NM_005297.3       AAGAGGGTGACCCGCACAGCCATCGCCATCTGTCTGGTCTTCTTTGTGTGCTGGGCACCC
                  ************************************************************

HsCD00511466      TACTATGTGCTACAGCTGACCCAGTTGTCCATCAGCCGCCCGACCCTCACCTTTGTCTAC
NM_005297.3       TACTATGTGCTACAGCTGACCCAGTTGTCCATCAGCCGCCCGACCCTCACCTTTGTCTAC
                  ************************************************************

HsCD00511466      TTATACAATGCGGCCATCAGCTTGGGCTATGCCAACAGCTGCCTCAACCCCTTTGTGTAC
NM_005297.3       TTATACAATGCGGCCATCAGCTTGGGCTATGCCAACAGCTGCCTCAACCCCTTTGTGTAC
                  ************************************************************

HsCD00511466      ATCGTGCTCTGTGAGACGTTCCGCAAACGCTTGGTCCTGTCGGTGAAGCCTGCAGCCCAG
NM_005297.3       ATCGTGCTCTGTGAGACGTTCCGCAAACGCTTGGTCCTGTCGGTGAAGCCTGCAGCCCAG
                  ************************************************************

HsCD00511466      GGGCAGCTTCGCGCTGTCAGCAACGCTCAGACGGCTGACGAGGAGAGGACAGAAAGCAAA
NM_005297.3       GGGCAGCTTCGCGCTGTCAGCAACGCTCAGACGGCTGACGAGGAGAGGACAGAAAGCAAA
                  ************************************************************

HsCD00511466      GGCACC---
NM_005297.3       GGCACCTGA
                  ******

Pathways

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

G alpha (i) signalling events

Reactome :

G alpha (q) signalling events

Reactome :

Gastrin-CREB signalling pathway via PKC and MAPK

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

Peptide ligand-binding receptors

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

WikiPathway :

GPCRs, Class A Rhodopsin-like

Protein

HPRD :

11874

Clone

Cloning Information :

611713

Original Clone ID :

ccsbBroadEn_06315

Organism

Species Specific ID:

2847

Genome

Chromosome :

Map Location :

22q13.2

Ensembl :

ENSG00000128285

Reagents

Labome :

MCHR1-antibody