DNASU Plasmid | RG9MTD1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TRMT10C

Gene Name: tRNA methyltransferase 10 homolog C (S. cerevisiae)

Original Clone ID:

ccsbBroadEn_12113

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC035967.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511760

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1192nts

Open reading frame: 1 to 1192

Reference Sequence Alignment

ATGAGTGTTAGTGTCAATTTCTTCAGACCTTTCACCAGGTTTTTGGTGCCATTTACCCTT
CATAGGAAGAGAAATAACTTAACAATTTTGCAGAGATACATGTCTTCCAAAATACCAGCT
GTTACTTATCCTAAAAATGAGAGTACACCCCCTTCTGAAGAGCTAGAGTTGGATAAGTGG
AAAACTACCATGAAATCTAGTGTGCAAGAAGAATGTGTTTCAACAATCTCAAGCAGTAAG
GATGAAGATCCTCTAGCTGCCACCAGAGAGTTCATTGAGATGTGGAGATTGCTTGGCAGA
GAAGTACCAGAACACATCACTGAAGAAGAGCTCAAAACCCTTATGGAATGTGTTTCTAAC
ACAGCAAAAAAAAAATATTTAAAATATTTATATACGAAGGAAAAAGTGAAAAAAGCTAGG
CAAATAAAAAAGGAAATGAAAGCAGCAGCAAGGGAAGAAGCAAAAAATATCAAGCTGCTA
GAAACCACTGAGGAAGATAAACAGAAAAACTTTCTATTTTTACGACTTTGGGATAGGAAT
ATGGACATAGCAATGGGCTGGAAGGGTGCCCAGGCCATGCAGTTTGGACAACCTTTGGTT
TTTGACATGGCTTACGAAAATTATATGAAACGAAAAGAATTGCAGAATACTGTTTCCCAG
CTTTTAGAAAGTGAAGGATGGAACAGAAGAAATGTTGATCCTTTCCATATTTATTTCTGC
AATCTAAAAATAGATGGTGCTTTGCACAGAGAGTTAGTTAAACGGTATCAAGAAAAATGG
GACAAATTGCTTTTAACATCAACAGAAAAGTCTCATGTAGATTTATTTCCAAAGGACAGT
ATTATCTATTTAACTGCAGATTCTCCCAATGTTATGACTACTTTCAGGCATGACAAAGTT
TATGTAATTGGGTCTTTTGTTGATAAGAGTATGCAGCCAGGCACATCCCTAGCCAAGGCA
AAACGGCTGAACCTGGCAACTGAATGCCTTCCATTAGATAAATATTTACAATGGGAAATT
GGTAACAAAAATCTCACCTTAGATCAAATGATACGTATTTTGTTATGTCTGAAAAACAAT
GGTAATTGGCAAGAGGCTCTGCAATTCGTTCCCAAGAGAAAACATACTGGTTTTCTGGAG
ATTTCTCAGCATTCTCAAGAGTTTATCAACAGACTAAAGAAGGCAAAGACT

Protein sequence predicted based on nucleotide sequence

MSVSVNFFRPFTRFLVPFTLHRKRNNLTILQRYMSSKIPAVTYPKNESTPPSEELELDKW
KTTMKSSVQEECVSTISSSKDEDPLAATREFIEMWRLLGREVPEHITEEELKTLMECVSN
TAKKKYLKYLYTKEKVKKARQIKKEMKAAAREEAKNIKLLETTEEDKQKNFLFLRLWDRN
MDIAMGWKGAQAMQFGQPLVFDMAYENYMKRKELQNTVSQLLESEGWNRRNVDPFHIYFC
NLKIDGALHRELVKRYQEKWDKLLLTSTEKSHVDLFPKDSIIYLTADSPNVMTTFRHDKV
YVIGSFVDKSMQPGTSLAKAKRLNLATECLPLDKYLQWEIGNKNLTLDQMIRILLCLKNN
GNWQEALQFVPKRKHTGFLEISQHSQEFINRLKKAKT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

TRMT10C

Symbol Nomenclature :

TRMT10C

SYNONYM :

HNYA; MRPP1; RG9MTD1

Designation :

HBV pre-S2 trans-regulated protein 2|RNA (guanine-9-) methyltransferase domain containing 1|mitochondrial RNase P subunit 1|mitochondrial ribonuclease P protein 1|renal carcinoma antigen NY-REN-49

Full Nomenclature :

tRNA methyltransferase 10 homolog C (S. cerevisiae)

GENEID :

54931

GenBank Accession :

HQ447662

HGNC :

26022

MIM :

615423

Vega :

OTTHUMG00000159114

Target GenBank:

HQ447662

Reference Sequence Alignment

  

HsCD00511760      ------------------ATGAGTGTTAGTGTCAATTTCTTCAGACCTTTCACCAGGTTT
NM_017819.3       ATGGCTGCTTTCCTCAAAATGAGTGTTAGTGTCAATTTCTTCAGACCTTTCACCAGGTTT
                                    ******************************************

HsCD00511760      TTGGTGCCATTTACCCTTCATAGGAAGAGAAATAACTTAACAATTTTGCAGAGATACATG
NM_017819.3       TTGGTGCCATTTACCCTTCATAGGAAGAGAAATAACTTAACAATTTTGCAGAGATACATG
                  ************************************************************

HsCD00511760      TCTTCCAAAATACCAGCTGTTACTTATCCTAAAAATGAGAGTACACCCCCTTCTGAAGAG
NM_017819.3       TCTTCCAAAATACCAGCTGTTACTTATCCTAAAAATGAGAGTACACCCCCTTCTGAAGAG
                  ************************************************************

HsCD00511760      CTAGAGTTGGATAAGTGGAAAACTACCATGAAATCTAGTGTGCAAGAAGAATGTGTTTCA
NM_017819.3       CTAGAGTTGGATAAGTGGAAAACTACCATGAAATCTAGTGTGCAAGAAGAATGTGTTTCA
                  ************************************************************

HsCD00511760      ACAATCTCAAGCAGTAAGGATGAAGATCCTCTAGCTGCCACCAGAGAGTTCATTGAGATG
NM_017819.3       ACAATCTCAAGCAGTAAGGATGAAGATCCTCTAGCTGCCACCAGAGAGTTCATTGAGATG
                  ************************************************************

HsCD00511760      TGGAGATTGCTTGGCAGAGAAGTACCAGAACACATCACTGAAGAAGAGCTCAAAACCCTT
NM_017819.3       TGGAGATTGCTTGGCAGAGAAGTACCAGAACACATCACTGAAGAAGAGCTCAAAACCCTT
                  ************************************************************

HsCD00511760      ATGGAATGTGTTTCTAACACAGCAAAAAAAAAATATTTAAAATATTTATATACGAAGGAA
NM_017819.3       ATGGAATGTGTTTCTAACACAGCAAAAAAAAAATATTTAAAATATTTATATACGAAGGAA
                  ************************************************************

HsCD00511760      AAAGTGAAAAAAGCTAGGCAAATAAAAAAGGAAATGAAAGCAGCAGCAAGGGAAGAAGCA
NM_017819.3       AAAGTGAAAAAAGCTAGGCAAATAAAAAAGGAAATGAAAGCAGCAGCAAGGGAAGAAGCA
                  ************************************************************

HsCD00511760      AAAAATATCAAGCTGCTAGAAACCACTGAGGAAGATAAACAGAAAAACTTTCTATTTTTA
NM_017819.3       AAAAATATCAAGCTGCTAGAAACCACTGAGGAAGATAAACAGAAAAACTTTCTATTTTTA
                  ************************************************************

HsCD00511760      CGACTTTGGGATAGGAATATGGACATAGCAATGGGCTGGAAGGGTGCCCAGGCCATGCAG
NM_017819.3       CGACTTTGGGATAGGAATATGGACATAGCAATGGGCTGGAAGGGTGCCCAGGCCATGCAG
                  ************************************************************

HsCD00511760      TTTGGACAACCTTTGGTTTTTGACATGGCTTACGAAAATTATATGAAACGAAAAGAATTG
NM_017819.3       TTTGGACAACCTTTGGTTTTTGACATGGCTTACGAAAATTATATGAAACGAAAAGAATTG
                  ************************************************************

HsCD00511760      CAGAATACTGTTTCCCAGCTTTTAGAAAGTGAAGGATGGAACAGAAGAAATGTTGATCCT
NM_017819.3       CAGAATACTGTTTCCCAGCTTTTAGAAAGTGAAGGATGGAACAGAAGAAATGTTGATCCT
                  ************************************************************

HsCD00511760      TTCCATATTTATTTCTGCAATCTAAAAATAGATGGTGCTTTGCACAGAGAGTTAGTTAAA
NM_017819.3       TTCCATATTTATTTCTGCAATCTAAAAATAGATGGTGCTTTGCACAGAGAGTTAGTTAAA
                  ************************************************************

HsCD00511760      CGGTATCAAGAAAAATGGGACAAATTGCTTTTAACATCAACAGAAAAGTCTCATGTAGAT
NM_017819.3       CGGTATCAAGAAAAATGGGACAAATTGCTTTTAACATCAACAGAAAAGTCTCATGTAGAT
                  ************************************************************

HsCD00511760      TTATTTCCAAAGGACAGTATTATCTATTTAACTGCAGATTCTCCCAATGTTATGACTACT
NM_017819.3       TTATTTCCAAAGGACAGTATTATCTATTTAACTGCAGATTCTCCCAATGTTATGACTACT
                  ************************************************************

HsCD00511760      TTCAGGCATGACAAAGTTTATGTAATTGGGTCTTTTGTTGATAAGAGTATGCAGCCAGGC
NM_017819.3       TTCAGGCATGACAAAGTTTATGTAATTGGGTCTTTTGTTGATAAGAGTATGCAGCCAGGC
                  ************************************************************

HsCD00511760      ACATCCCTAGCCAAGGCAAAACGGCTGAACCTGGCAACTGAATGCCTTCCATTAGATAAA
NM_017819.3       ACATCCCTAGCCAAGGCAAAACGGCTGAACCTGGCAACTGAATGCCTTCCATTAGATAAA
                  ************************************************************

HsCD00511760      TATTTACAATGGGAAATTGGTAACAAAAATCTCACCTTAGATCAAATGATACGTATTTTG
NM_017819.3       TATTTACAATGGGAAATTGGTAACAAAAATCTCACCTTAGATCAAATGATACGTATTTTG
                  ************************************************************

HsCD00511760      TTATGTCTGAAAAACAATGGTAATTGGCAAGAGGCTCTGCAATTCGTTCCCAAGAGAAAA
NM_017819.3       TTATGTCTGAAAAACAATGGTAATTGGCAAGAGGCTCTGCAATTCGTTCCCAAGAGAAAA
                  ************************************************************

HsCD00511760      CATACTGGTTTTCTGGAGATTTCTCAGCATTCTCAAGAGTTTATCAACAGACTAAAGAAG
NM_017819.3       CATACTGGTTTTCTGGAGATTTCTCAGCATTCTCAAGAGTTTATCAACAGACTAAAGAAG
                  ************************************************************

HsCD00511760      GCAAAGACT---
NM_017819.3       GCAAAGACTTAA
                  *********