DNASU Plasmid | SNAPC3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: SNAPC3

Gene Name: small nuclear RNA activating complex, polypeptide 3, 50kDa

Original Clone ID:

ccsbBroadEn_01562

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC121011.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511781

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1234nts

Open reading frame: 1 to 1234

Reference Sequence Alignment

ATGGCTGAAGGAAGCCGAGGTGGCCCTACGTGTAGCGGGGTGGGTGGCAGGCAGGACCCA
GTCTCCGGCAGTGGCGGCTGCAACTTTCCAGAGTATGAGCTTCCCGAGCTAAATACGCGC
GCTTTCCATGTGGGCGCCTTTGGGGAGCTGTGGCGGGGCCGTCTGCGCGGGGCCGGGGAC
TTGTCGCTGAGGGAGCCGCCGGCATCCGCTCTGCCTGGGAGCCAGGCAGCTGACTCCGAC
CGGGAGGATGCCGCGGTGGCCAGGGATCTGGACTGCAGCCTGGAGGCGGCGGCTGAGCTG
AGGGCGGTGTGCGGCCTTGATAAACTGAAATGCCTTGAGGACGGTGAGGATCCAGAAGTC
ATTCCGGAGAATACTGACCTGGTGACTTTGGGGGTTAGAAAAAGGTTCTTGGAACATCGG
GAAGAAACCATTACAATAGATCGAGCCTGCAGACAAGAAACATTCGTTTATGAGATGGAG
TCACATGCCATAGGAAAAAAGCCTGAAAATTCAGCAGACATGATTGAAGAAGGGGAGCTT
ATCCTATCTGTGAATATCTTGTACCCTGTTATATTTCATAAGCACAAAGAACACAAACCA
TACCAAACAATGCTGGTGTTGGGCAGTCAAAAACTCACACAACTGAGGGATTCAATTCGA
TGTGTCAGTGACCTCCAGATTGGTGGTGAATTCAGCAACACTCCTGACCAAGCCCCTGAG
CACATCAGCAAAGACCTATACAAATCAGCCTTCTTTTATTTTGAAGGAACATTTTATAAT
GATAAAAGATACCCAGAATGCAGAGATTTGAGCAGAACTATCATTGAGTGGTCAGAGTCC
CATGATAGAGGCTATGGAAAGTTTCAGACTGCTAGAATGGAAGATTTCACCTTCAATGAC
TTGTGTATTAAACTGGGTTTTCCTTACTTATACTGTCATCAGGGAGACTGTGAACATGTC
ATTGTCATTACTGACATAAGGCTTGTGCATCATGATGACTGCTTGGATAGGACATTGTAT
CCCCTCCTTATCAAGAAGCATTGGCTATGGACCAGAAAATGTTTTGTTTGTAAAATGTAT
ACAGCCAGATGGGTGACGAACAATGACAGTTTTGCACCAGAGGACCCATGCTTCTTTTGT
GATGTTTGCTTCCGAATGCTGCACTATGATTCAGAAGGCAACAAACTGGGGGAATTCCTT
GCTTATCCTTATGTTGATCCTGGAACCTTTAAT

Protein sequence predicted based on nucleotide sequence

MAEGSRGGPTCSGVGGRQDPVSGSGGCNFPEYELPELNTRAFHVGAFGELWRGRLRGAGD
LSLREPPASALPGSQAADSDREDAAVARDLDCSLEAAAELRAVCGLDKLKCLEDGEDPEV
IPENTDLVTLGVRKRFLEHREETITIDRACRQETFVYEMESHAIGKKPENSADMIEEGEL
ILSVNILYPVIFHKHKEHKPYQTMLVLGSQKLTQLRDSIRCVSDLQIGGEFSNTPDQAPE
HISKDLYKSAFFYFEGTFYNDKRYPECRDLSRTIIEWSESHDRGYGKFQTARMEDFTFND
LCIKLGFPYLYCHQGDCEHVIVITDIRLVHHDDCLDRTLYPLLIKKHWLWTRKCFVCKMY
TARWVTNNDSFAPEDPCFFCDVCFRMLHYDSEGNKLGEFLAYPYVDPGTFN

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

SNAPC3

Symbol Nomenclature :

SNAPC3

SYNONYM :

PTFbeta; SNAP50

Designation :

Full Nomenclature :

small nuclear RNA activating complex, polypeptide 3, 50kDa

GENEID :

6619

Locus Tag :

RP11-307K19.1

GenBank Accession :

EU446705

HGNC :

11136

MIM :

602348

Vega :

OTTHUMG00000019583

Target GenBank:

EU446705

Reference Sequence Alignment

  

HsCD00511781        ATGGCTGAAGGAAGCCGAGGTGGCCCTACGTGTAGCGGGGTGGGTGGCAGGCAGGACCCA
NM_001039697.1      ATGGCTGAAGGAAGCCGAGGTGGCCCTACGTGTAGCGGGGTGGGTGGCAGGCAGGACCCA
                    ************************************************************

HsCD00511781        GTCTCCGGCAGTGGCGGCTGCAACTTTCCAGAGTATGAGCTTCCCGAGCTAAATACGCGC
NM_001039697.1      GTCTCCGGCAGTGGCGGCTGCAACTTTCCAGAGTATGAGCTTCCCGAGCTAAATACGCGC
                    ************************************************************

HsCD00511781        GCTTTCCATGTGGGCGCCTTTGGGGAGCTGTGGCGGGGCCGTCTGCGCGGGGCCGGGGAC
NM_001039697.1      GCTTTCCATGTGGGCGCCTTTGGGGAGCTGTGGCGGGGCCGTCTGCGCGGGGCCGGGGAC
                    ************************************************************

HsCD00511781        TTGTCGCTGAGGGAGCCGCCGGCATCCGCTCTGCCTGGGAGCCAGGCAGCTGACTCCGAC
NM_001039697.1      TTGTCGCTGAGGGAGCCGCCGGCATCCGCTCTGCCTGGGAGCCAGGCAGCTGACTCCGAC
                    ************************************************************

HsCD00511781        CGGGAGGATGCCGCGGTGGCCAGGGATCTGGACTGCAGCCTGGAGGCGGCGGCTGAGCTG
NM_001039697.1      CGGGAGGATGCCGCGGTGGCCAGGGATCTGGACTGCAGCCTGGAGGCGGCGGCTGAGCTG
                    ************************************************************

HsCD00511781        AGGGCGGTGTGCGGCCTTGATAAACTGAAATGCCTTGAGGACGGTGAGGATCCAGAAGTC
NM_001039697.1      AGGGCGGTGTGCGGCCTTGATAAACTGAAATGCCTTGAGGACGGTGAGGATCCAGAAGTC
                    ************************************************************

HsCD00511781        ATTCCGGAGAATACTGACCTGGTGACTTTGGGGGTTAGAAAAAGGTTCTTGGAACATCGG
NM_001039697.1      ATTCCGGAGAATACTGACCTGGTGACTTTGGGGGTTAGAAAAAGGTTCTTGGAACATCGG
                    ************************************************************

HsCD00511781        GAAGAAACCATTACAATAGATCGAGCCTGCAGACAAGAAACATTCGTTTATGAGATGGAG
NM_001039697.1      GAAGAAACCATTACAATAGATCGAGCCTGCAGACAAGAAACATTCGTTTATGAGATGGAG
                    ************************************************************

HsCD00511781        TCACATGCCATAGGAAAAAAGCCTGAAAATTCAGCAGACATGATTGAAGAAGGGGAGCTT
NM_001039697.1      TCACATGCCATAGGAAAAAAGCCTGAAAATTCAGCAGACATGATTGAAGAAGGGGAGCTT
                    ************************************************************

HsCD00511781        ATCCTATCTGTGAATATCTTGTACCCTGTTATATTTCATAAGCACAAAGAACACAAACCA
NM_001039697.1      ATCCTATCTGTGAATATCTTGTACCCTGTTATATTTCATAAGCACAAAGAACACAAACCA
                    ************************************************************

HsCD00511781        TACCAAACAATGCTGGTGTTGGGCAGTCAAAAACTCACACAACTGAGGGATTCAATTCGA
NM_001039697.1      TACCAAACAATGCTGGTGTTGGGCAGTCAAAAACTCACACAACTGAGGGATTCAATTCGA
                    ************************************************************

HsCD00511781        TGTGTCAGTGACCTCCAGATTGGTGGTGAATTCAGCAACACTCCTGACCAAGCCCCTGAG
NM_001039697.1      TGTGTCAGTGACCTCCAGATTGGTGGTGAATTCAGCAACACTCCTGACCAAGCCCCTGAG
                    ************************************************************

HsCD00511781        CACATCAGCAAAGACCTATACAAATCAGCCTTCTTTTATTTTGAAGGAACATTTTATAAT
NM_001039697.1      CACATCAGCAAAGACCTATACAAATCAGCCTTCTTTTATTTTGAAGGAACATTTTATAAT
                    ************************************************************

HsCD00511781        GATAAAAGATACCCAGAATGCAGAGATTTGAGCAGAACTATCATTGAGTGGTCAGAGTCC
NM_001039697.1      GATAAAAGATACCCAGAATGCAGAGATTTGAGCAGAACTATCATTGAGTGGTCAGAGTCC
                    ************************************************************

HsCD00511781        CATGATAGAGGCTATGGAAAGTTTCAGACTGCTAGAATGGAAGATTTCACCTTCAATGAC
NM_001039697.1      CATGATAGAGGCTATGGAAAGTTTCAGACTGCTAGAATGGAAGATTTCACCTTCAATGAC
                    ************************************************************

HsCD00511781        TTGTGTATTAAACTGGGTTTTCCTTACTTATACTGTCATCAGGGAGACTGTGAACATGTC
NM_001039697.1      TTGTGTATTAAACTGGGTTTTCCTTACTTATACTGTCATCAGGGAGACTGTGAACATGTC
                    ************************************************************

HsCD00511781        ATTGTCATTACTGACATAAGGCTTGTGCATCATGATGACTGCTTGGATAGGACATTGTAT
NM_001039697.1      ATTGTCATTACTGACATAAGGCTTGTGCATCATGATGACTGCTTGGATAGGACATTGTAT
                    ************************************************************

HsCD00511781        CCCCTCCTTATCAAGAAGCATTGGCTATGGACCAGAAAATGTTTTGTTTGTAAAATGTAT
NM_001039697.1      CCCCTCCTTATCAAGAAGCATTGGCTATGGACCAGAAAATGTTTTGTTTGTAAAATGTAT
                    ************************************************************

HsCD00511781        ACAGCCAGATGGGTGACGAACAATGACAGTTTTGCACCAGAGGACCCATGCTTCTTTTGT
NM_001039697.1      ACAGCCAGATGGGTGACGAACAATGACAGTTTTGCACCAGAGGACCCATGCTTCTTTTGT
                    ************************************************************

HsCD00511781        GATGTTTGCTTCCGAATGCTGCACTATGATTCAGAAGGCAACAAACTGGGGGAATTCCTT
NM_001039697.1      GATGTTTGCTTCCGAATGCTGCACTATGATTCAGAAGGCAACAAACTGGGGGAATTCCTT
                    ************************************************************

HsCD00511781        GCTTATCCTTATGTTGATCCTGGAACCTTTAAT---
NM_001039697.1      GCTTATCCTTATGTTGATCCTGGAACCTTTAATTAA
                    *********************************

Pathways

Reactome :

Gene Expression

Reactome :

RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription

Reactome :

RNA Polymerase III Abortive And Retractive Initiation

Reactome :

RNA Polymerase III Transcription

Reactome :

RNA Polymerase III Transcription Initiation

Reactome :

RNA Polymerase III Transcription Initiation From Type 3 Promoter

Reactome :

Transcription

Protein

HPRD :

03826

Clone

Cloning Information :

605086

Original Clone ID :

ccsbBroadEn_01562

Organism

Species Specific ID:

6619

Genome

Chromosome :

Map Location :

9p22.3

Ensembl :

ENSG00000164975

Reagents

Labome :

SNAPC3-antibody