DNASU Plasmid | TMPRSS11A (H. sapiens ) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TMPRSS11A

Gene Name: transmembrane protease, serine 11A

Original Clone ID:

ccsbBroadEn_10020

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC111796.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511799

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1255nts

Open reading frame: 1 to 1255

Reference Sequence Alignment

ATGATGTATCGGACAGTAGGATTTGGCACCCGAAGCAGAAATCTGAAGCCATGGATGATT
GCCGTTCTCATTGTGTTGTCCCTGACAGTGGTGGCAGTGACCATAGGTCTCCTGGTTCAC
TTCCTAGTATTTGACCAAAAAAAGGAGTACTATCATGGCTCCTTTAAAATTTTAGATCCA
CAAATCAATAACAATTTCGGACAAAGCAACACATATCAACTTAAGGACTTACGAGAGACG
ACCGAAAATTTGGTGGATGAGATATTTATAGATTCAGCCTGGAAGAAAAATTATATCAAG
AACCAAGTAGTCAGACTGACTCCAGAGGAAGATGGTGTGAAAGTAGATGTCATTATGGTG
TTCCAGTTCCCCTCTACTGAACAAAGGGCAGTAAGAGAGAAGAAAATCCAAAGCATCTTA
AATCAGAAGATAAGGAATTTAAGAGCCTTGCCAATAAATGCCTCATCAGTTCAAGTTAAT
GCAATGAGCTCATCAACAGGGGAGTTAACTGTCCAAGCAAGTTGTGGTAAACGAGTTGTT
CCATTAAACGTCAACAGAATAGCATCTGGAGTCATTGCACCCAAGGCGGCCTGGCCTTGG
CAAGCTTCCCTTCAGTATGATAACATCCATCAGTGTGGGGCCACCTTGATTAGTAACACA
TGGCTTGTCACTGCAGCACACTGCTTCCAGAAGTATAAAAATCCACATCAATGGACTGTT
AGTTTTGGAACAAAAATCAACCCTCCCTTAATGAAAAGAAATGTCAGAAGATTTATTATC
CATGAGAAGTACCGCTCTGCAGCAAGAGAGTACGACATTGCTGTTGTGCAGGTCTCTTCC
AGAGTCACCTTTTCGGATGACATACGCCGGATTTGTTTGCCAGAAGCCTCTGCATCCTTC
CAACCAAATTTGACTGTCCACATCACAGGATTTGGAGCACTTTACTATGGTGGGGAATCC
CAAAATGATCTCCGAGAAGCCAGAGTGAAAATCATAAGTGACGATGTCTGCAAGCAACCA
CAGGTGTATGGCAATGATATAAAACCTGGAATGTTCTGTGCCGGATATATGGAAGGAATT
TATGATGCCTGCAGGGGTGATTCTGGGGGACCTTTAGTCACAAGGGATCTGAAAGATACG
TGGTATCTCATTGGAATTGTAAGCTGGGGAGATAACTGTGGTCAAAAGGACAAGCCTGGA
GTCTACACACAAGTGACTTATTACCGAAACTGGATTGCTTCAAAAACAGGCATC

Protein sequence predicted based on nucleotide sequence

MMYRTVGFGTRSRNLKPWMIAVLIVLSLTVVAVTIGLLVHFLVFDQKKEYYHGSFKILDP
QINNNFGQSNTYQLKDLRETTENLVDEIFIDSAWKKNYIKNQVVRLTPEEDGVKVDVIMV
FQFPSTEQRAVREKKIQSILNQKIRNLRALPINASSVQVNAMSSSTGELTVQASCGKRVV
PLNVNRIASGVIAPKAAWPWQASLQYDNIHQCGATLISNTWLVTAAHCFQKYKNPHQWTV
SFGTKINPPLMKRNVRRFIIHEKYRSAAREYDIAVVQVSSRVTFSDDIRRICLPEASASF
QPNLTVHITGFGALYYGGESQNDLREARVKIISDDVCKQPQVYGNDIKPGMFCAGYMEGI
YDACRGDSGGPLVTRDLKDTWYLIGIVSWGDNCGQKDKPGVYTQVTYYRNWIASKTGI

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TMPRSS11A

Symbol Nomenclature :

TMPRSS11A

SYNONYM :

ECRG1

Designation :

airway trypsin-like protease 1|epidermal type II transmembrane serine protease|epidermal type-II transmembrane serine protease|esophageal cancer-susceptibility gene 1 protein|transmembrane protease serine 11A

Full Nomenclature :

transmembrane protease, serine 11A

GENEID :

339967

HGNC :

27954

MIM :

611704

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00511799        ATGATGTATCGGACAGTAGGATTTGGCACCCGAAGCAGAAATCTGAAGCCATGGATGATT
NM_182606.3         ATGATGTATCGGACAGTGGGATTTGGCACCCGAAGCAGAAATCTGAAGCCATGGATGATT
NM_001114387.1      ATGATGTATCGGACAGTGGGATTTGGCACCCGAAGCAGAAATCTGAAGCCATGGATGATT
                    *****************.******************************************

HsCD00511799        GCCGTTCTCATTGTGTTGTCCCTGACAGTGGTGGCAGTGACCATAGGTCTCCTGGTTCAC
NM_182606.3         GCCGTTCTCATTGTGTTGTCCCTGACAGTGGTGGCAGTGACCATAGGTCTCCTGGTTCAC
NM_001114387.1      GCCGTTCTCATTGTGTTGTCCCTGACAGTGGTGGCAGTGACCATAGGTCTCCTGGTTCAC
                    ************************************************************

HsCD00511799        TTCCTAGTATTTGACCAAAAAAAGGAGTACTATCATGGCTCCTTTAAAATTTTAGATCCA
NM_182606.3         TTCCTAGTATTTGACCAAAAAAAGGAGTACTATCATGGCTCCTTTAAAATTTTAGATCCA
NM_001114387.1      TTCCTAGTATTTGACCAAAAAAAGGAGTACTATCATGGCTCCTTTAAAATTTTAGATCCA
                    ************************************************************

HsCD00511799        CAAATCAATAACAATTTCGGACAAAGCAACACATATCAACTTAAGGACTTACGAGAGACG
NM_182606.3         CAAATCAATAACAATTTCGGACAAAGCAACACATATCAACTTAAGGACTTACGAGAGACG
NM_001114387.1      CAAATCAATAACAATTTCGGACAAAGCAACACATATCAACTTAAGGACTTACGAGAGACG
                    ************************************************************

HsCD00511799        ACCGAAAATTTG---------GTGGATGAGATATTTATAGATTCAGCCTGGAAGAAAAAT
NM_182606.3         ACCGAAAATTTGGTGAGTCAGGTGGATGAGATATTTATAGATTCAGCCTGGAAGAAAAAT
NM_001114387.1      ACCGAAAATTTG---------GTGGATGAGATATTTATAGATTCAGCCTGGAAGAAAAAT
                    ************         ***************************************

HsCD00511799        TATATCAAGAACCAAGTAGTCAGACTGACTCCAGAGGAAGATGGTGTGAAAGTAGATGTC
NM_182606.3         TATATCAAGAACCAAGTAGTCAGACTGACTCCAGAGGAAGATGGTGTGAAAGTAGATGTC
NM_001114387.1      TATATCAAGAACCAAGTAGTCAGACTGACTCCAGAGGAAGATGGTGTGAAAGTAGATGTC
                    ************************************************************

HsCD00511799        ATTATGGTGTTCCAGTTCCCCTCTACTGAACAAAGGGCAGTAAGAGAGAAGAAAATCCAA
NM_182606.3         ATTATGGTGTTCCAGTTCCCCTCTACTGAACAAAGGGCAGTAAGAGAGAAGAAAATCCAA
NM_001114387.1      ATTATGGTGTTCCAGTTCCCCTCTACTGAACAAAGGGCAGTAAGAGAGAAGAAAATCCAA
                    ************************************************************

HsCD00511799        AGCATCTTAAATCAGAAGATAAGGAATTTAAGAGCCTTGCCAATAAATGCCTCATCAGTT
NM_182606.3         AGCATCTTAAATCAGAAGATAAGGAATTTAAGAGCCTTGCCAATAAATGCCTCATCAGTT
NM_001114387.1      AGCATCTTAAATCAGAAGATAAGGAATTTAAGAGCCTTGCCAATAAATGCCTCATCAGTT
                    ************************************************************

HsCD00511799        CAAGTTAATGCAATGAGCTCATCAACAGGGGAGTTAACTGTCCAAGCAAGTTGTGGTAAA
NM_182606.3         CAAGTTAATGCAATGAGCTCATCAACAGGGGAGTTAACTGTCCAAGCAAGTTGTGGTAAA
NM_001114387.1      CAAGTTAATGCAATGAGCTCATCAACAGGGGAGTTAACTGTCCAAGCAAGTTGTGGTAAA
                    ************************************************************

HsCD00511799        CGAGTTGTTCCATTAAACGTCAACAGAATAGCATCTGGAGTCATTGCACCCAAGGCGGCC
NM_182606.3         CGAGTTGTTCCATTAAACGTCAACAGAATAGCATCTGGAGTCATTGCACCCAAGGCGGCC
NM_001114387.1      CGAGTTGTTCCATTAAACGTCAACAGAATAGCATCTGGAGTCATTGCACCCAAGGCGGCC
                    ************************************************************

HsCD00511799        TGGCCTTGGCAAGCTTCCCTTCAGTATGATAACATCCATCAGTGTGGGGCCACCTTGATT
NM_182606.3         TGGCCTTGGCAAGCTTCCCTTCAGTATGATAACATCCATCAGTGTGGGGCCACCTTGATT
NM_001114387.1      TGGCCTTGGCAAGCTTCCCTTCAGTATGATAACATCCATCAGTGTGGGGCCACCTTGATT
                    ************************************************************

HsCD00511799        AGTAACACATGGCTTGTCACTGCAGCACACTGCTTCCAGAAGTATAAAAATCCACATCAA
NM_182606.3         AGTAACACATGGCTTGTCACTGCAGCACACTGCTTCCAGAAGTATAAAAATCCACATCAA
NM_001114387.1      AGTAACACATGGCTTGTCACTGCAGCACACTGCTTCCAGAAGTATAAAAATCCACATCAA
                    ************************************************************

HsCD00511799        TGGACTGTTAGTTTTGGAACAAAAATCAACCCTCCCTTAATGAAAAGAAATGTCAGAAGA
NM_182606.3         TGGACTGTTAGTTTTGGAACAAAAATCAACCCTCCCTTAATGAAAAGAAATGTCAGAAGA
NM_001114387.1      TGGACTGTTAGTTTTGGAACAAAAATCAACCCTCCCTTAATGAAAAGAAATGTCAGAAGA
                    ************************************************************

HsCD00511799        TTTATTATCCATGAGAAGTACCGCTCTGCAGCAAGAGAGTACGACATTGCTGTTGTGCAG
NM_182606.3         TTTATTATCCATGAGAAGTACCGCTCTGCAGCAAGAGAGTACGACATTGCTGTTGTGCAG
NM_001114387.1      TTTATTATCCATGAGAAGTACCGCTCTGCAGCAAGAGAGTACGACATTGCTGTTGTGCAG
                    ************************************************************

HsCD00511799        GTCTCTTCCAGAGTCACCTTTTCGGATGACATACGCCGGATTTGTTTGCCAGAAGCCTCT
NM_182606.3         GTCTCTTCCAGAGTCACCTTTTCGGATGACATACGCCAGATTTGTTTGCCAGAAGCCTCT
NM_001114387.1      GTCTCTTCCAGAGTCACCTTTTCGGATGACATACGCCAGATTTGTTTGCCAGAAGCCTCT
                    *************************************.**********************

HsCD00511799        GCATCCTTCCAACCAAATTTGACTGTCCACATCACAGGATTTGGAGCACTTTACTATGGT
NM_182606.3         GCATCCTTCCAACCAAATTTGACTGTCCACATCACAGGATTTGGAGCACTTTACTATGGT
NM_001114387.1      GCATCCTTCCAACCAAATTTGACTGTCCACATCACAGGATTTGGAGCACTTTACTATGGT
                    ************************************************************

HsCD00511799        GGGGAATCCCAAAATGATCTCCGAGAAGCCAGAGTGAAAATCATAAGTGACGATGTCTGC
NM_182606.3         GGGGAATCCCAAAATGATCTCCGAGAAGCCAGAGTGAAAATCATAAGTGATGATGTCTGC
NM_001114387.1      GGGGAATCCCAAAATGATCTCCGAGAAGCCAGAGTGAAAATCATAAGTGATGATGTCTGC
                    ************************************************** *********

HsCD00511799        AAGCAACCACAGGTGTATGGCAATGATATAAAACCTGGAATGTTCTGTGCCGGATATATG
NM_182606.3         AAGCAACCACAGGTGTATGGCAATGATATAAAACCTGGAATGTTCTGTGCCGGATATATG
NM_001114387.1      AAGCAACCACAGGTGTATGGCAATGATATAAAACCTGGAATGTTCTGTGCCGGATATATG
                    ************************************************************

HsCD00511799        GAAGGAATTTATGATGCCTGCAGGGGTGATTCTGGGGGACCTTTAGTCACAAGGGATCTG
NM_182606.3         GAAGGAATTTATGATGCCTGCAGGGGTGATTCTGGGGGACCTTTAGTCACAAGGGATCTG
NM_001114387.1      GAAGGAATTTATGATGCCTGCAGGGGTGATTCTGGGGGACCTTTAGTCACAAGGGATCTG
                    ************************************************************

HsCD00511799        AAAGATACGTGGTATCTCATTGGAATTGTAAGCTGGGGAGATAACTGTGGTCAAAAGGAC
NM_182606.3         AAAGATACGTGGTATCTCATTGGAATTGTAAGCTGGGGAGATAACTGTGGTCAAAAGGAC
NM_001114387.1      AAAGATACGTGGTATCTCATTGGAATTGTAAGCTGGGGAGATAACTGTGGTCAAAAGGAC
                    ************************************************************

HsCD00511799        AAGCCTGGAGTCTACACACAAGTGACTTATTACCGAAACTGGATTGCTTCAAAAACAGGC
NM_182606.3         AAGCCTGGAGTCTACACACAAGTGACTTATTACCGAAACTGGATTGCTTCAAAAACAGGC
NM_001114387.1      AAGCCTGGAGTCTACACACAAGTGACTTATTACCGAAACTGGATTGCTTCAAAAACAGGC
                    ************************************************************

HsCD00511799        ATC---
NM_182606.3         ATCTAA
NM_001114387.1      ATCTAA
                    ***