DNASU Plasmid | GCM1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: GCM1

Gene Name: glial cells missing homolog 1 (Drosophila)

Original Clone ID:

ccsbBroadEn_01947

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC096288.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511977

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1308nts

Open reading frame: 1 to 1308

Reference Sequence Alignment

ATGGAACCTGACGACTTTGATTCTGAAGACAAAGAGATATTAAGCTGGGATATTAATGAT
GTGAAACTGCCACAGAACGTGAAAAAAACCGACTGGTTCCAGGAGTGGCCAGATTCCTAT
GCCAAACACATCTACAGCTCGGAGGACAAGAATGCGCAGCGGCACCTGAGCAGCTGGGCC
ATGCGCAATACCAACAACCACAACTCCCGCATCCTCAAGAAGTCCTGCCTGGGTGTGGTG
GTGTGCGGCCGCGACTGTCTCGCAGAGGAGGGGCGCAAGATCTACCTGAGACCTGCCATC
TGTGACAAGGCCCGGCAGAAGCAGCAGCGGAAACGCTGTCCCAACTGTGACGGGCCTCTG
AAGCTCATCCCTTGCCGAGGTCATGGGGGCTTCCCGGTCACCAACTTCTGGAGGCACGAC
GGACGCTTTATATTTTTCCAGTCAAAGGGAGAGCATGATCATCCAAAACCAGAAACCAAG
TTAGAAGCTGAGGCAAGAAGAGCCATGAAGAAAGTGAACACAGCACCTTCCTCCGTCTCA
TTGAGCCTGAAGGGGAGCACAGAGACCAGGTCTCTTCCAGGTGAAACACAAAGTCAGGGG
AGTTTACCTTTAACTTGGTCTTTCCAGGAAGGCGTCCAATTGCCTGGTAGTTACAGTGGA
CATTTAATAGCTAACACTCCTCAGCAGAACTCACTAAATGATTGCTTTTCCTTCTCCAAG
AGTTATGGTCTGGGAGGAATCACAGATCTGACTGACCAGACTTCCACTGTGGACCCCATG
AAGCTCTATGAAAAGCGCAAATTGTCCAGTAGCAGAACCTACAGTAGTGGAGACCTGCTT
CCTCCTTCTGCCTCCGGAGTCTACTCTGATCATGGCGATCTACAAGCGTGGAGTAAAAAT
GCTGCTTTGGGGAGAAATCATCTTGCTGACAACTGTTATTCCAATTATCCTTTTCCTCTG
ACCAGCTGGCCTTGCAGCTTCTCTCCTTCCCAAAACTCTTCAGAACCCTTTTACCAGCAG
CTTCCATTGGAGCCACCTGCAGCCAAAACTGGCTGTCCCCCATTATGGCCAAATCCAGCG
GGTAATCTTTATGAAGAGAAAGTACATGTGGATTTTAACAGCTACGTCCAGTCTCCTGCA
TACCATTCACCTCAAGAAGACCCCTTTCTCTTCACCTACGCCTCTCATCCTCATCAGCAA
TATTCACTGCCAAGCAAGAGCAGCAAATGGGATTTTGAGGAAGAAATGACATACTTGGGT
TTGGATCACTGCAACAATGATATGCTTCTGAACCTGTGTCCTTTGAGA

Protein sequence predicted based on nucleotide sequence

MEPDDFDSEDKEILSWDINDVKLPQNVKKTDWFQEWPDSYAKHIYSSEDKNAQRHLSSWA
MRNTNNHNSRILKKSCLGVVVCGRDCLAEEGRKIYLRPAICDKARQKQQRKRCPNCDGPL
KLIPCRGHGGFPVTNFWRHDGRFIFFQSKGEHDHPKPETKLEAEARRAMKKVNTAPSSVS
LSLKGSTETRSLPGETQSQGSLPLTWSFQEGVQLPGSYSGHLIANTPQQNSLNDCFSFSK
SYGLGGITDLTDQTSTVDPMKLYEKRKLSSSRTYSSGDLLPPSASGVYSDHGDLQAWSKN
AALGRNHLADNCYSNYPFPLTSWPCSFSPSQNSSEPFYQQLPLEPPAAKTGCPPLWPNPA
GNLYEEKVHVDFNSYVQSPAYHSPQEDPFLFTYASHPHQQYSLPSKSSKWDFEEEMTYLG
LDHCNNDMLLNLCPLR

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

GCM1

Symbol Nomenclature :

GCM1

SYNONYM :

GCMA; hGCMa

Designation :

GCM motif protein 1|chorion-specific transcription factor GCMa

Full Nomenclature :

glial cells missing homolog 1 (Drosophila)

GENEID :

8521

HGNC :

4197

MIM :

603715

Vega :

OTTHUMG00000014871

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00511977      ATGGAACCTGACGACTTTGATTCTGAAGACAAAGAGATATTAAGCTGGGATATTAATGAT
NM_003643.3       ATGGAACCTGACGACTTTGATTCTGAAGACAAAGAGATATTAAGCTGGGATATTAATGAT
                  ************************************************************

HsCD00511977      GTGAAACTGCCACAGAACGTGAAAAAAACCGACTGGTTCCAGGAGTGGCCAGATTCCTAT
NM_003643.3       GTGAAACTGCCACAGAACGTGAAAAAAACCGACTGGTTCCAGGAGTGGCCAGATTCCTAT
                  ************************************************************

HsCD00511977      GCCAAACACATCTACAGCTCGGAGGACAAGAATGCGCAGCGGCACCTGAGCAGCTGGGCC
NM_003643.3       GCCAAACACATCTACAGCTCGGAGGACAAGAATGCGCAGCGGCACCTGAGCAGCTGGGCC
                  ************************************************************

HsCD00511977      ATGCGCAATACCAACAACCACAACTCCCGCATCCTCAAGAAGTCCTGCCTGGGTGTGGTG
NM_003643.3       ATGCGCAATACCAACAACCACAACTCCCGCATCCTCAAGAAGTCCTGCCTGGGTGTGGTG
                  ************************************************************

HsCD00511977      GTGTGCGGCCGCGACTGTCTCGCAGAGGAGGGGCGCAAGATCTACCTGAGACCTGCCATC
NM_003643.3       GTGTGCGGCCGCGACTGTCTCGCAGAGGAGGGGCGCAAGATCTACCTGAGACCTGCCATC
                  ************************************************************

HsCD00511977      TGTGACAAGGCCCGGCAGAAGCAGCAGCGGAAACGCTGTCCCAACTGTGACGGGCCTCTG
NM_003643.3       TGTGACAAGGCCCGGCAGAAGCAGCAGCGGAAACGCTGTCCCAACTGTGACGGGCCTCTG
                  ************************************************************

HsCD00511977      AAGCTCATCCCTTGCCGAGGTCATGGGGGCTTCCCGGTCACCAACTTCTGGAGGCACGAC
NM_003643.3       AAGCTCATCCCTTGCCGAGGTCATGGGGGCTTCCCGGTCACCAACTTCTGGAGGCACGAC
                  ************************************************************

HsCD00511977      GGACGCTTTATATTTTTCCAGTCAAAGGGAGAGCATGATCATCCAAAACCAGAAACCAAG
NM_003643.3       GGACGCTTTATATTTTTCCAGTCAAAGGGAGAGCATGATCATCCAAAACCAGAAACCAAG
                  ************************************************************

HsCD00511977      TTAGAAGCTGAGGCAAGAAGAGCCATGAAGAAAGTGAACACAGCACCTTCCTCCGTCTCA
NM_003643.3       TTAGAAGCTGAGGCAAGAAGAGCCATGAAGAAAGTGAACACAGCACCTTCCTCCGTCTCA
                  ************************************************************

HsCD00511977      TTGAGCCTGAAGGGGAGCACAGAGACCAGGTCTCTTCCAGGTGAAACACAAAGTCAGGGG
NM_003643.3       TTGAGCCTGAAGGGGAGCACAGAGACCAGGTCTCTTCCAGGTGAAACACAAAGTCAGGGG
                  ************************************************************

HsCD00511977      AGTTTACCTTTAACTTGGTCTTTCCAGGAAGGCGTCCAATTGCCTGGTAGTTACAGTGGA
NM_003643.3       AGTTTACCTTTAACTTGGTCTTTCCAGGAAGGCGTCCAATTGCCTGGTAGTTACAGTGGA
                  ************************************************************

HsCD00511977      CATTTAATAGCTAACACTCCTCAGCAGAACTCACTAAATGATTGCTTTTCCTTCTCCAAG
NM_003643.3       CATTTAATAGCTAACACTCCTCAGCAGAACTCACTAAATGATTGCTTTTCCTTCTCCAAG
                  ************************************************************

HsCD00511977      AGTTATGGTCTGGGAGGAATCACAGATCTGACTGACCAGACTTCCACTGTGGACCCCATG
NM_003643.3       AGTTATGGTCTGGGAGGAATCACAGATCTGACTGACCAGACTTCCACTGTGGACCCCATG
                  ************************************************************

HsCD00511977      AAGCTCTATGAAAAGCGCAAATTGTCCAGTAGCAGAACCTACAGTAGTGGAGACCTGCTT
NM_003643.3       AAGCTCTATGAAAAGCGCAAATTGTCCAGTAGCAGAACCTACAGTAGTGGAGACCTGCTT
                  ************************************************************

HsCD00511977      CCTCCTTCTGCCTCCGGAGTCTACTCTGATCATGGCGATCTACAAGCGTGGAGTAAAAAT
NM_003643.3       CCTCCTTCTGCCTCCGGAGTCTACTCTGATCATGGCGATCTACAAGCGTGGAGTAAAAAT
                  ************************************************************

HsCD00511977      GCTGCTTTGGGGAGAAATCATCTTGCTGACAACTGTTATTCCAATTATCCTTTTCCTCTG
NM_003643.3       GCTGCTTTGGGGAGAAATCATCTTGCTGACAACTGTTATTCCAATTATCCTTTTCCTCTG
                  ************************************************************

HsCD00511977      ACCAGCTGGCCTTGCAGCTTCTCTCCTTCCCAAAACTCTTCAGAACCCTTTTACCAGCAG
NM_003643.3       ACCAGCTGGCCTTGCAGCTTCTCTCCTTCCCAAAACTCTTCAGAACCCTTTTACCAGCAG
                  ************************************************************

HsCD00511977      CTTCCATTGGAGCCACCTGCAGCCAAAACTGGCTGTCCCCCATTATGGCCAAATCCAGCG
NM_003643.3       CTTCCATTGGAGCCACCTGCAGCCAAAACTGGCTGTCCCCCATTATGGCCAAATCCAGCG
                  ************************************************************

HsCD00511977      GGTAATCTTTATGAAGAGAAAGTACATGTGGATTTTAACAGCTACGTCCAGTCTCCTGCA
NM_003643.3       GGTAATCTTTATGAAGAGAAAGTACATGTGGATTTTAACAGCTACGTCCAGTCTCCTGCA
                  ************************************************************

HsCD00511977      TACCATTCACCTCAAGAAGACCCCTTTCTCTTCACCTACGCCTCTCATCCTCATCAGCAA
NM_003643.3       TACCATTCACCTCAAGAAGACCCCTTTCTCTTCACCTACGCCTCTCATCCTCATCAGCAA
                  ************************************************************

HsCD00511977      TATTCACTGCCAAGCAAGAGCAGCAAATGGGATTTTGAGGAAGAAATGACATACTTGGGT
NM_003643.3       TATTCACTGCCAAGCAAGAGCAGCAAATGGGATTTTGAGGAAGAAATGACATACTTGGGT
                  ************************************************************

HsCD00511977      TTGGATCACTGCAACAATGATATGCTTCTGAACCTGTGTCCTTTGAGA---
NM_003643.3       TTGGATCACTGCAACAATGATATGCTTCTGAACCTGTGTCCTTTGAGATGA
                  ************************************************