DNASU Plasmid | HFM1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: HFM1

Gene Name: HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae)

Original Clone ID:

ccsbBroadEn_13338

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC132823.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512184

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1414nts

Open reading frame: 1 to 1414

Reference Sequence Alignment

ATGCTGAAATCAAATGATTGCCTGTTTTCTTTGGAAAATTTGTTTTTTGAAAAACCAGAT
GAAGTTGAAAACCATCCAGACAATGAAAAGTCATTGGATTGGTTTCTCCCTCCTGCTCCA
TTGATTTCAGAAATTCCAGATACTCAGGAGTTAGAGGAAGAATTAGAAAGTCATAAACTG
TTAGGTCAGGAAAAGAGGCCAAAAATGTTAACATCAAATTTAAAGATAACTAATGAAGAT
ACAAATTATATTTCACTAACACAAAAATTCCAGTTTGCCTTTCCTTCTGATAAATATGAA
CAGGATGATCTAAATTTAGAAGGGGTAGGTAATAATGACTTATCACATGTTGCTGGCAAG
CTGACATATGCTTCTCAGAAATATAAAAATCACATTGGCACTGAGATAGCACCTGAGAAG
AGTGTTCCTGATGATACAAAATTAGTTAATTTTGCAGAAGATAAAGGAGAGAGCACATCA
GTATTCCGGAAAAGATTATTTAAAATATCTGACAATATACATGGGAGTGCTTATTCTAAT
GACAATGAATTGGACTCTCACATTGGCTCAGTGAAAATTGTACAAACAGAAATGAACAAA
GGGAAATCAAGGAACTATAGCAATAGTAAGCAGAAATTTCAGTATTCTGCAAATGTGTTT
ACAGCAAATAATGCTTTTTCTGCTTCTGAAATCGGAGAAGGCATGTTCAAAGCACCATCT
TTTTCAGTTGCTTTCCAACCTCATGATATTCAAGAGGTAACAGAAAATGGTTTAGGTTCC
TTGAAGGCTGTCACAGAAATTCCGGCAAAATTTAGAAGTATTTTCAAAGAATTTCCATAT
TTCAACTATATACAGTCCAAGGCCTTTGATGATCTTCTTTACACAGATAGGAATTTTGTG
ATTTGTGCTCCAACTGGTTCTGGAAAAACTGTAGTGTTTGAACTAGCTATAACAAGATTG
TTAATGGAAGTACCATTGCCATGGTTGAATATTAAAATTGTTTACATGGCACCAATAAAA
GCCTTGTGCAGTCAGCGTTTTGATGACTGGAAAGAAAAATTTGGACCAATAGGATTGAAT
TGTAAAGAACTTACTGGAGATACAGTAATGGATGATCTATTTGAGATTCAGCATGCCCAT
ATTATTATGACAACTCCAGAAAAATGGGATAGCATGACTAGGAAATGGAGAGACAACTCT
TTGGTTCAGCTGGTTCGACTGTTTCTCATTGATGAGGTACATATTGTAAAAGATGAAAAT
CGTGGTCCAACTCTTGAAGTTGTAGTTAGCAGAATGAAAACTGTACAGTCTGTTTCTCAG
ACTTTAAAAAATACCAGCACTGCTATTCCAATGCGATTTGTAGCTGTATCTGCAACAATT
CCAAATGCTGAGGATGTGAGTTACAATTTTAAC

Protein sequence predicted based on nucleotide sequence

MLKSNDCLFSLENLFFEKPDEVENHPDNEKSLDWFLPPAPLISEIPDTQELEEELESHKL
LGQEKRPKMLTSNLKITNEDTNYISLTQKFQFAFPSDKYEQDDLNLEGVGNNDLSHVAGK
LTYASQKYKNHIGTEIAPEKSVPDDTKLVNFAEDKGESTSVFRKRLFKISDNIHGSAYSN
DNELDSHIGSVKIVQTEMNKGKSRNYSNSKQKFQYSANVFTANNAFSASEIGEGMFKAPS
FSVAFQPHDIQEVTENGLGSLKAVTEIPAKFRSIFKEFPYFNYIQSKAFDDLLYTDRNFV
ICAPTGSGKTVVFELAITRLLMEVPLPWLNIKIVYMAPIKALCSQRFDDWKEKFGPIGLN
CKELTGDTVMDDLFEIQHAHIIMTTPEKWDSMTRKWRDNSLVQLVRLFLIDEVHIVKDEN
RGPTLEVVVSRMKTVQSVSQTLKNTSTAIPMRFVAVSATIPNAEDVSYNFN

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

HFM1

Symbol Nomenclature :

HFM1

SYNONYM :

MER3; RP11-539G11.1; SEC63D1; Si-11; Si-11-6; helicase

Designation :

SEC63 domain containing 1|SEC63 domain-containing protein 1|helicase-like protein HFM1|probable ATP-dependent DNA helicase HFM1

Full Nomenclature :

HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae)

GENEID :

164045

Locus Tag :

RP11-509K22.1

HGNC :

20193

Vega :

OTTHUMG00000010093

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00512184        ATGCTGAAATCAAATGATTGCCTGTTTTCTTTGGAAAATTTGTTTTTTGAAAAACCAGAT
NM_001017975.3      ATGCTGAAATCAAATGATTGCCTGTTTTCTTTGGAAAATTTGTTTTTTGAAAAACCAGAT
                    ************************************************************

HsCD00512184        GAAGTTGAAAACCATCCAGACAATGAAAAGTCATTGGATTGGTTTCTCCCTCCTGCTCCA
NM_001017975.3      GAAGTTGAAAACCATCCAGACAATGAAAAGTCATTGGATTGGTTTCTCCCTCCTGCTCCA
                    ************************************************************

HsCD00512184        TTGATTTCAGAAATTCCAGATACTCAGGAGTTAGAGGAAGAATTAGAAAGTCATAAACTG
NM_001017975.3      TTGATTTCAGAAATTCCAGATACTCAGGAGTTAGAGGAAGAATTAGAAAGTCATAAACTG
                    ************************************************************

HsCD00512184        TTAGGTCAGGAAAAGAGGCCAAAAATGTTAACATCAAATTTAAAGATAACTAATGAAGAT
NM_001017975.3      TTAGGTCAGGAAAAGAGGCCAAAAATGTTAACATCAAATTTAAAGATAACTAATGAAGAT
                    ************************************************************

HsCD00512184        ACAAATTATATTTCACTAACACAAAAATTCCAGTTTGCCTTTCCTTCTGATAAATATGAA
NM_001017975.3      ACAAATTATATTTCACTAACACAAAAATTCCAGTTTGCCTTTCCTTCTGATAAATATGAA
                    ************************************************************

HsCD00512184        CAGGATGATCTAAATTTAGAAGGGGTAGGTAATAATGACTTATCACATGTTGCTGGCAAG
NM_001017975.3      CAGGATGATCTAAATTTAGAAGGGGTAGGTAATAATGACTTATCACATATTGCTGGCAAG
                    ************************************************.***********

HsCD00512184        CTGACATATGCTTCTCAGAAATATAAAAATCACATTGGCACTGAGATAGCACCTGAGAAG
NM_001017975.3      CTGACATATGCTTCTCAGAAATATAAAAATCACATTGGCACTGAGATAGCACCTGAGAAG
                    ************************************************************

HsCD00512184        AGTGTTCCTGATGATACAAAATTAGTTAATTTTGCAGAAGATAAAGGAGAGAGCACATCA
NM_001017975.3      AGTGTTCCTGATGATACAAAATTAGTTAATTTTGCAGAAGATAAAGGAGAGAGCACATCA
                    ************************************************************

HsCD00512184        GTATTCCGGAAAAGATTATTTAAAATATCTGACAATATACATGGGAGTGCTTATTCTAAT
NM_001017975.3      GTATTCCGGAAAAGATTATTTAAAATATCTGACAATATACATGGGAGTGCTTATTCTAAT
                    ************************************************************

HsCD00512184        GACAATGAATTGGACTCTCACATTGGCTCAGTGAAAATTGTACAAACAGAAATGAACAAA
NM_001017975.3      GACAATGAATTGGACTCTCACATTGGCTCAGTGAAAATTGTACAAACAGAAATGAACAAA
                    ************************************************************

HsCD00512184        GGGAAATCAAGGAACTATAGCAATAGTAAGCAGAAATTTCAGTATTCTGCAAATGTGTTT
NM_001017975.3      GGGAAATCAAGGAACTATAGCAATAGTAAGCAAAAATTTCAGTATTCTGCAAATGTGTTT
                    ********************************.***************************

HsCD00512184        ACAGCAAATAATGCTTTTTCTGCTTCTGAAATCGGAGAAGGCATGTTCAAAGCACCATCT
NM_001017975.3      ACAGCAAATAATGCTTTTTCTGCTTCTGAAATCGGAGAAGGCATGTTCAAAGCACCATCT
                    ************************************************************

HsCD00512184        TTTTCAGTTGCTTTCCAACCTCATGATATTCAAGAGGTAACAGAAAATGGTTTAGGTTCC
NM_001017975.3      TTTTCAGTTGCTTTCCAACCTCATGATATTCAAGAGGTAACAGAAAATGGTTTAGGTTCC
                    ************************************************************

HsCD00512184        TTGAAGGCTGTCACAGAAATTCCGGCAAAATTTAGAAGTATTTTCAAAGAATTTCCATAT
NM_001017975.3      TTGAAGGCTGTCACAGAAATTCCGGCAAAATTTAGAAGTATTTTCAAAGAATTTCCATAT
                    ************************************************************

HsCD00512184        TTCAACTATATACAGTCCAAGGCCTTTGATGATCTTCTTTACACAGATAGGAATTTTGTG
NM_001017975.3      TTCAACTATATACAGTCCAAGGCCTTTGATGATCTTCTTTACACAGATAGGAATTTTGTG
                    ************************************************************

HsCD00512184        ATTTGTGCTCCAACTGGTTCTGGAAAAACTGTAGTGTTTGAACTAGCTATAACAAGATTG
NM_001017975.3      ATTTGTGCTCCAACTGGTTCTGGAAAAACTGTAGTGTTTGAACTAGCTATAACAAGATTG
                    ************************************************************

HsCD00512184        TTAATGGAAGTACCATTGCCATGGTTGAATATTAAAATTGTTTACATGGCACCAATAAAA
NM_001017975.3      TTAATGGAAGTACCATTGCCATGGTTGAATATTAAAATTGTTTACATGGCACCAATAAAA
                    ************************************************************

HsCD00512184        GCCTTGTGCAGTCAGCGTTTTGATGACTGGAAAGAAAAATTTGGACCAATAGGATTGAAT
NM_001017975.3      GCCTTGTGCAGTCAGCGTTTTGATGACTGGAAAGAAAAATTTGGACCAATAGGATTGAAT
                    ************************************************************

HsCD00512184        TGTAAAGAACTTACTGGAGATACAGTAATGGATGATCTATTTGAGATTCAGCATGCCCAT
NM_001017975.3      TGTAAAGAACTTACTGGAGATACAGTAATGGATGATCTATTTGAGATTCAGCATGCCCAT
                    ************************************************************

HsCD00512184        ATTATTATGACAACTCCAGAAAAATGGGATAGCATGACTAGGAAATGGAGAGACAACTCT
NM_001017975.3      ATTATTATGACAACTCCAGAAAAATGGGATAGCATGACTAGGAAATGGAGAGACAACTCT
                    ************************************************************

HsCD00512184        TTGGTTCAGCTGGTTCGACTGTTTCTCATTGATGAGGTACATATTGTAAAAGATGAAAAT
NM_001017975.3      TTGGTTCAGCTGGTTCGACTGTTTCTCATTGATGAGGTACATATTGTAAAAGATGAAAAT
                    ************************************************************

HsCD00512184        CGTGGTCCAACTCTTGAAGTTGTAGTTAGCAGAATGAAAACTGTACAGTCTGTTTCTCAG
NM_001017975.3      CGTGGTCCAACTCTTGAAGTTGTAGTTAGCAGAATGAAAACTGTACAGTCTGTTTCTCAG
                    ************************************************************

HsCD00512184        ACTTTAAAAAATACCAGCACTGCTATTCCAATGCGATTTGTAGCTGTATCTGCAACAATT
NM_001017975.3      ACTTTAAAAAATACCAGCACTGCTATTCCAATGCGATTTGTAGCTGTATCTGCAACAATT
                    ************************************************************

HsCD00512184        CCAAATGCTGAGGATGTGAGTTACAATTTTAAC---------------------------
NM_001017975.3      CCAAATGCTGAGGATATTGCAGAATGGCTTTCAGATGGTGAAAGACCAGCTGTGTGTCTG
                    ***************.* . : *.:.  **:..                           

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      AAAATGGATGAGAGCCATAGACCAGTGAAACTTCAGAAAGTGGTCCTTGGATTTCCCTGC
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      AGTAGTAACCAAACTGAGTTTAAGTTTGATTTAACCCTCAACTACAAAATTGCCAGTGTT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      ATACAAATGTACTCTGATCAGAAACCCACACTTGTGTTTTGTGCAACAAGGAAGGGTGTG
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      CAACAGGCTGCTTCTGTTCTTGTGAAAGATGCTAAATTTATTATGACTGTGGAACAGAAA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      CAGAGGTTACAGAAGTATGCATATTCCGTAAGAGATTCAAAACTGAGAGATATCTTAAAA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GATGGTGCTGCTTATCATCATGCTGGTATGGAGCTGTCAGATAGAAAAGTAGTTGAGGGA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GCTTTTACTGTTGGAGATTTACCAGTTCTTTTTACTACCAGTACTTTAGCTATGGGAGTA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      AATTTGCCTGCTCACCTAGTAGTTATAAAATCTACAATGCATTATGCTGGAGGACTGTTT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GAAGAGTACAGTGAAACAGATATTCTACAGATGATTGGTAGAGCTGGTCGACCTCAATTT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GACACTACAGCTACTGCAGTTATCATGACTCGATTAAGCACAAGGGACAAGTACATTCAG
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      ATGTTAGCTTGTAGAGACACTGTAGAAAGCAGTTTGCACAGACATCTTATTGAACATTTA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      AATGCAGAGATAGTACTGCATACCATCACGGATGTGAATATTGCTGTGGAATGGATACGA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      TCAACTCTGCTTTATATCAGAGCCTTGAAAAATCCATCTCATTATGGTTTTGCATCTGGA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      TTGAACAAAGATGGAATTGAAGCAAAATTACAAGAATTATGTTTGAAGAATCTGAATGAT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      TTATCATCCCTGGACTTAATAAAGATGGATGAAGGTGTTAATTTCAAACCAACTGAAGCA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GGAAGATTGATGGCTTGGTATTATATTACATTTGAGACAGTGAAGAAATTTTATACAATC
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      AGTGGAAAAGAAACCTTATCAGATCTGGTTACATTGATAGCTGGCTGCAAGGAATTTCTA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GATATACAGTTAAGGATAAATGAAAAGAAAACACTGAATACTTTGAACAAAGATCCAAAT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      CGGATAACTATCAGATTTCCAATGGAAGGAAGAATTAAAACAAGAGAAATGAAAGTAAAT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      TGTCTTATTCAGGCTCAACTAGGATGCATTCCCATACAAGATTTTGCTTTGACACAAGAT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      ACCGCAAAGATTTTCAGACATGGCTCCCGAATTACAAGATGGTTGTCAGATTTTGTAGCT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GCTCAAGAAAAGAAGTTTGCTGTACTATTGAATAGTTTGATTTTAGCTAAATGTTTTAGG
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      TGTAAACTTTGGGAAAACTCTCTGCATGTATCCAAACAACTGGAAAAAATTGGTATAACA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      TTGTCAAATGCAATCGTAAATGCTGGTTTGACTTCCTTTAAAAAAATAGAAGAGACAGAT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GCAAGGGAACTTGAATTGATTTTAAACAGACATCCCCCCTTTGGAACCCAGATAAAAGAA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      ACTGTGATGTATCTACCAAAATATGAACTTAAAGTGGAACAGATTACAAGATATAGTGAT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      ACGACGGCAGAAATATTAGTGACTGTTATATTAAGAAATTTTGAACAGCTACAAACTAAA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      AGAACAGCATCGGATTCTCACTATGTTACCTTAATCATAGGTGACGCAGATAATCAAGTA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GTTTATCTGCACAAGATTACGGATTCTGTTTTGCTAAAAGCTGGAAGTTGGGCTAAAAAG
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      ATTGCTGTGAAAAGAGCTCTTAAATCTGAAGATCTTAGCATAAATCTAATAAGTTCTGAA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      TTTGTTGGGCTTGATATTCAGCAGAAACTTACAGTCTTTTACTTAGAACCCAAGAGGTTT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GGAAATCAAATCACTATGCAAAGAAAATCTGAAACACAGATTTCCCATTCTAAACATTCA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GACATATCTACAATAGCAGGACCTAATAAAGGAACGACTGCCAGCAAAAAACCTGGGAAC
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      CGAGAATGCAATCATCTTTGTAAAAGTAAACATACATGTGGACATGACTGCTGTAAAATT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GGAGTTGCACAGAAGTCAGAAATTAAAGAGTCAACAATTTCTTCATATTTATCTGATTTA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      AGAAACAGGAATGCTGTTTCATCTGTTCCTCCAGTTAAACGTCTGAAGATACAGATGAAT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      AAATCTCAAAGTGTGGACCTTAAAGAGTTTGGTTTTACTCCAAAACCTTCCCTTCCTAGT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      ATATCAAGGTCAGAATATTTAAACATATCTGAATTGCCTATAATGGAGCAGTGGGATCAG
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      CCTGAAATCTATGGAAAAGTTAGACAAGAACCATCTGAATATCAAGACAAAGAAGTTTTG
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      AATGTGAACTTTGAATTGGGAAATGAAGTTTGGGATGATTTTGATGATGAAAACTTAGAA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GTTACTAGCTTTTCAACTGATACTGAGAAGACAAAAATATCAGGATTTGGAAACACTTTG
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      AGTTCAAGTACCAGGGGAAGTAAGCTACCCCTTCAAGAGTCAAAGAGCAAATTCCAAAGA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GAAATGTCAAACAGTTTTGTTTCATCACATGAGATGTCGGATATTTCTTTATCAAATTCT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GCTATGCCCAAGTTCAGTGCATCCTCCATGACAAAATTACCTCAACAAGCCGGAAATGCA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      GTTATTGTCCATTTTCAAGAAAGAAAACCACAAAATCTGTCACCAGAGATTGAGAAGCAA
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      TGCTTTACTTTCTCTGAAAAAAACCCAAATTCTTCAAATTATAAAAAAGTGGATTTTTTT
                                                                                

HsCD00512184        ------------------------------------------------------------
NM_001017975.3      ATTAGAAACAGTGAATGTAAAAAGGAAGTTGATTTCAGTATGTATCATCCTGATGATGAA
                                                                                

HsCD00512184        ------------------------------------------------
NM_001017975.3      GCTGATGAAATGAAGTCTTTATTGGGAATATTTGATGGTATTTTCTAA