DNASU Plasmid | PPIL4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: PPIL4

Gene Name: peptidylprolyl isomerase (cyclophilin)-like 4

Original Clone ID:

ccsbBroadEn_04476

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC020986.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512420

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1477nts

Open reading frame: 1 to 1477

Reference Sequence Alignment

ATGGCGGTTCTACTGGAGACCACTTTAGGCGACGTCGTCATCGACTTGTACACCGAAGAA
CGGCCGCGTGCCTGCTTGAATTTCTTGAAACTGTGCAAAATAAAATATTACAATTATTGC
CTTATTCACAATGTACAGAGGGATTTTATCATACAAACTGGCGATCCTACAGGGACTGGC
CGTGGAGGAGAGTCTATCTTTGGCCAACTGTATGGTGATCAAGCAAGCTTTTTTGAGGCA
GAAAAAGTCCCAAGAATTAAGCACAAGAAGAAAGGCACAGTGTCCATGGTGAATAATGGC
AGTGATCAACATGGATCTCAGTTTCTTATCACCACAGGAGAAAATCTAGATTATCTTGAT
GGTGTCCATACGGTGTTTGGTGAGGTGACAGAAGGCATGGACATAATTAAGAAAATTAAT
GAGACCTTTGTTGACAAGGACTTTGTACCATATCAGGATATCAGGATAAATCATACGGTG
ATTTTAGATGATCCATTTGATGACCCTCCTGATTTATTAATCCCTGATCGATCACCAGAA
CCTACAAGGGAACAATTAGATAGTGGTCGAATAGGAGCAGATGAAGAAATTGATGATTTC
AAAGGAAGATCAGCTGAGGAAGTAGAAGAAATAAAGGCAGAAAAAGAGGCTAAAACTCAG
GCTATACTTTTGGAGATGGTGGGAGACCTACCTGATGCAGATATTAAACCTCCAGAAAAT
GTACTGTTTGTGTGTAAATTGAACCCAGTGACCACAGATGAGGATCTGGAAATAATATTC
TCTAGATTTGGGCCAATAAGAAGTTGTGAAGTTATCCGAGACTGGAAGACAGGAGAGTCC
CTCTGTTACGCTTTTATTGAATTTGAAAAGGAAGAAGATTGTGAGAAAGCATTCTTCAAA
ATGGACAATGTGCTTATAGATGACAGAAGAATACATGTGGATTTTAGCCAGTCGGTTGCA
AAGGTTAAATGGAAAGGAAAAGGTGGGAAATACACCAAGAGTGATTTCAAGGAGTATGAA
AAAGAACAGGATAAACCACCTAATTTGGTTCTGAAAGATAAAGTAAAGCCCAAACAGGAT
ACAAAATACGATCTTATATTAGATGAGCAGGCCGAAGACTCAAAATCAAGTCACTCACAC
ACAAGTAAAAAACACAAGAAGAAAACCCATCACTGTTCTGAAGAGAAAGAAGATGAGGAC
TACATGCCAATCAAAAATACTAATCAGGATATCTATAGAGAAATGGGGTTTGGTCACTAT
GAAGAAGAAGAAAGCTGTTGGGAGAAACAAAAGAGTGAAAAGAGAGACCGAACTCAGAAC
CGAAGTCGTAGCCGATCTCGAGAGAGGGATGGCCATTATAGTAATAGTCATAAATCAAAA
TACCAAACAGATCTTTATGAAAGAGAAAGGAGTAAAAAGAGAGACCGAAGCAGAAGTCCA
AAGAAGTCCAAAGATAAAGAAAAATCTAAGTATAGA

Protein sequence predicted based on nucleotide sequence

MAVLLETTLGDVVIDLYTEERPRACLNFLKLCKIKYYNYCLIHNVQRDFIIQTGDPTGTG
RGGESIFGQLYGDQASFFEAEKVPRIKHKKKGTVSMVNNGSDQHGSQFLITTGENLDYLD
GVHTVFGEVTEGMDIIKKINETFVDKDFVPYQDIRINHTVILDDPFDDPPDLLIPDRSPE
PTREQLDSGRIGADEEIDDFKGRSAEEVEEIKAEKEAKTQAILLEMVGDLPDADIKPPEN
VLFVCKLNPVTTDEDLEIIFSRFGPIRSCEVIRDWKTGESLCYAFIEFEKEEDCEKAFFK
MDNVLIDDRRIHVDFSQSVAKVKWKGKGGKYTKSDFKEYEKEQDKPPNLVLKDKVKPKQD
TKYDLILDEQAEDSKSSHSHTSKKHKKKTHHCSEEKEDEDYMPIKNTNQDIYREMGFGHY
EEEESCWEKQKSEKRDRTQNRSRSRSRERDGHYSNSHKSKYQTDLYERERSKKRDRSRSP
KKSKDKEKSKYR

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

PPIL4

Symbol Nomenclature :

PPIL4

SYNONYM :

HDCME13P

Designation :

Full Nomenclature :

peptidylprolyl isomerase (cyclophilin)-like 4

GENEID :

85313

Locus Tag :

RP11-703H16.1

GenBank Accession :

GQ129365

HGNC :

15702

MIM :

607609

Vega :

OTTHUMG00000015788

Target GenBank:

GQ129365

Reference Sequence Alignment

  

HsCD00512420      ATGGCGGTTCTACTGGAGACCACTTTAGGCGACGTCGTCATCGACTTGTACACCGAAGAA
NM_139126.3       ATGGCGGTTCTACTGGAGACCACTTTAGGCGACGTCGTCATCGACTTGTACACCGAAGAA
                  ************************************************************

HsCD00512420      CGGCCGCGTGCCTGCTTGAATTTCTTGAAACTGTGCAAAATAAAATATTACAATTATTGC
NM_139126.3       CGGCCGCGTGCCTGCTTGAATTTCTTGAAACTGTGCAAAATAAAATATTACAATTATTGC
                  ************************************************************

HsCD00512420      CTTATTCACAATGTACAGAGGGATTTTATCATACAAACTGGCGATCCTACAGGGACTGGC
NM_139126.3       CTTATTCACAATGTACAGAGGGATTTTATCATACAAACTGGCGATCCTACAGGGACTGGC
                  ************************************************************

HsCD00512420      CGTGGAGGAGAGTCTATCTTTGGCCAACTGTATGGTGATCAAGCAAGCTTTTTTGAGGCA
NM_139126.3       CGTGGAGGAGAGTCTATCTTTGGCCAACTGTATGGTGATCAAGCAAGCTTTTTTGAGGCA
                  ************************************************************

HsCD00512420      GAAAAAGTCCCAAGAATTAAGCACAAGAAGAAAGGCACAGTGTCCATGGTGAATAATGGC
NM_139126.3       GAAAAAGTCCCAAGAATTAAGCACAAGAAGAAAGGCACAGTGTCCATGGTGAATAATGGC
                  ************************************************************

HsCD00512420      AGTGATCAACATGGATCTCAGTTTCTTATCACCACAGGAGAAAATCTAGATTATCTTGAT
NM_139126.3       AGTGATCAACATGGATCTCAGTTTCTTATCACCACAGGAGAAAATCTAGATTATCTTGAT
                  ************************************************************

HsCD00512420      GGTGTCCATACGGTGTTTGGTGAGGTGACAGAAGGCATGGACATAATTAAGAAAATTAAT
NM_139126.3       GGTGTCCATACGGTGTTTGGTGAGGTGACAGAAGGCATGGACATAATTAAGAAAATTAAT
                  ************************************************************

HsCD00512420      GAGACCTTTGTTGACAAGGACTTTGTACCATATCAGGATATCAGGATAAATCATACGGTG
NM_139126.3       GAGACCTTTGTTGACAAGGACTTTGTACCATATCAGGATATCAGGATAAATCATACGGTG
                  ************************************************************

HsCD00512420      ATTTTAGATGATCCATTTGATGACCCTCCTGATTTATTAATCCCTGATCGATCACCAGAA
NM_139126.3       ATTTTAGATGATCCATTTGATGACCCTCCTGATTTATTAATCCCTGATCGATCACCAGAA
                  ************************************************************

HsCD00512420      CCTACAAGGGAACAATTAGATAGTGGTCGAATAGGAGCAGATGAAGAAATTGATGATTTC
NM_139126.3       CCTACAAGGGAACAATTAGATAGTGGTCGAATAGGAGCAGATGAAGAAATTGATGATTTC
                  ************************************************************

HsCD00512420      AAAGGAAGATCAGCTGAGGAAGTAGAAGAAATAAAGGCAGAAAAAGAGGCTAAAACTCAG
NM_139126.3       AAAGGAAGATCAGCTGAGGAAGTAGAAGAAATAAAGGCAGAAAAAGAGGCTAAAACTCAG
                  ************************************************************

HsCD00512420      GCTATACTTTTGGAGATGGTGGGAGACCTACCTGATGCAGATATTAAACCTCCAGAAAAT
NM_139126.3       GCTATACTTTTGGAGATGGTGGGAGACCTACCTGATGCAGATATTAAACCTCCAGAAAAT
                  ************************************************************

HsCD00512420      GTACTGTTTGTGTGTAAATTGAACCCAGTGACCACAGATGAGGATCTGGAAATAATATTC
NM_139126.3       GTACTGTTTGTGTGTAAATTGAACCCAGTGACCACAGATGAGGATCTGGAAATAATATTC
                  ************************************************************

HsCD00512420      TCTAGATTTGGGCCAATAAGAAGTTGTGAAGTTATCCGAGACTGGAAGACAGGAGAGTCC
NM_139126.3       TCTAGATTTGGGCCAATAAGAAGTTGTGAAGTTATCCGAGACTGGAAGACAGGAGAGTCC
                  ************************************************************

HsCD00512420      CTCTGTTACGCTTTTATTGAATTTGAAAAGGAAGAAGATTGTGAGAAAGCATTCTTCAAA
NM_139126.3       CTCTGTTACGCTTTTATTGAATTTGAAAAGGAAGAAGATTGTGAGAAAGCATTCTTCAAA
                  ************************************************************

HsCD00512420      ATGGACAATGTGCTTATAGATGACAGAAGAATACATGTGGATTTTAGCCAGTCGGTTGCA
NM_139126.3       ATGGACAATGTGCTTATAGATGACAGAAGAATACATGTGGATTTTAGCCAGTCGGTTGCA
                  ************************************************************

HsCD00512420      AAGGTTAAATGGAAAGGAAAAGGTGGGAAATACACCAAGAGTGATTTCAAGGAGTATGAA
NM_139126.3       AAGGTTAAATGGAAAGGAAAAGGTGGGAAATACACCAAGAGTGATTTCAAGGAGTATGAA
                  ************************************************************

HsCD00512420      AAAGAACAGGATAAACCACCTAATTTGGTTCTGAAAGATAAAGTAAAGCCCAAACAGGAT
NM_139126.3       AAAGAACAGGATAAACCACCTAATTTGGTTCTGAAAGATAAAGTAAAGCCCAAACAGGAT
                  ************************************************************

HsCD00512420      ACAAAATACGATCTTATATTAGATGAGCAGGCCGAAGACTCAAAATCAAGTCACTCACAC
NM_139126.3       ACAAAATACGATCTTATATTAGATGAGCAGGCCGAAGACTCAAAATCAAGTCACTCACAC
                  ************************************************************

HsCD00512420      ACAAGTAAAAAACACAAGAAGAAAACCCATCACTGTTCTGAAGAGAAAGAAGATGAGGAC
NM_139126.3       ACAAGTAAAAAACACAAGAAGAAAACCCATCACTGTTCTGAAGAGAAAGAAGATGAGGAC
                  ************************************************************

HsCD00512420      TACATGCCAATCAAAAATACTAATCAGGATATCTATAGAGAAATGGGGTTTGGTCACTAT
NM_139126.3       TACATGCCAATCAAAAATACTAATCAGGATATCTATAGAGAAATGGGGTTTGGTCACTAT
                  ************************************************************

HsCD00512420      GAAGAAGAAGAAAGCTGTTGGGAGAAACAAAAGAGTGAAAAGAGAGACCGAACTCAGAAC
NM_139126.3       GAAGAAGAAGAAAGCTGTTGGGAGAAACAAAAGAGTGAAAAGAGAGACCGAACTCAGAAC
                  ************************************************************

HsCD00512420      CGAAGTCGTAGCCGATCTCGAGAGAGGGATGGCCATTATAGTAATAGTCATAAATCAAAA
NM_139126.3       CGAAGTCGTAGCCGATCTCGAGAGAGGGATGGCCATTATAGTAATAGTCATAAATCAAAA
                  ************************************************************

HsCD00512420      TACCAAACAGATCTTTATGAAAGAGAAAGGAGTAAAAAGAGAGACCGAAGCAGAAGTCCA
NM_139126.3       TACCAAACAGATCTTTATGAAAGAGAAAGGAGTAAAAAGAGAGACCGAAGCAGAAGTCCA
                  ************************************************************

HsCD00512420      AAGAAGTCCAAAGATAAAGAAAAATCTAAGTATAGA---
NM_139126.3       AAGAAGTCCAAAGATAAAGAAAAATCTAAGTATAGATGA
                  ************************************