DNASU Plasmid | ZNF689 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF689

Gene Name: zinc finger protein 689

Original Clone ID:

ccsbBroadEn_04683

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC014000.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512536

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1501nts

Open reading frame: 1 to 1501

Reference Sequence Alignment

ATGGCGCCACCTTCGGCTCCGCTCCCTGCGCAGGGACCAGGAAAGGCCAGACCCAGTCGG
AAAAGGGGCAGGAGGCCGAGGGCTCTGAAGTTCGTGGACGTGGCCGTGTACTTCTCCCCG
GAGGAGTGGGGCTGCCTGCGGCCCGCGCAGAGGGCCCTGTACCGGGACGTGATGCGGGAG
ACCTACGGTCACCTGGGCGCGCTCGGGTGCGCAGGTCCCAAACCAGCCCTCATCTCCTGG
TTGGAACGAAACACCGATGACTGGGAACCGGCTGCTCTAGATCCGCAGGAGTACCCGAGA
GGGCTAACAGTCCAGAGAAAAAGCAGAACCAGAAAGAAGAATGGGGAGAAGGAAGTATTC
CCGCCTAAGGAGGCACCCCGAAAGGGGAAGCGAGGCCGGAGGCCCAGCAAACCCCGACTG
ATTCCTAGGCAGACGTCCGGGGGCCCCATCTGCCCTGACTGCGGCTGTACCTTCCCTGAT
CATCAGGCCCTGGAGAGCCACAAGTGCGCCCAGAATCTAAAAAAGCCTTACCCTTGCCCA
GACTGTGGGCGCCGCTTTTCCTATCCATCCCTGCTGGTCAGTCACCGGCGGGCACACTCC
GGCGAGTGCCCCTATGTTTGTGACCAGTGTGGCAAACGTTTCTCCCAGCGCAAGAACCTC
TCCCAGCACCAGGTCATCCATACAGGGGAGAAGCCCTATCACTGCCCTGACTGTGGTCGC
TGCTTCCGGAGGAGCCGGTCCTTGGCCAATCACCGGACCACACACACAGGTGAAAAACCC
CACCAGTGCCCTAGCTGTGGACGTCGCTTCGCCTACCCCTCCCTGCTAGCCATCCACCAG
CGTACACACACGGGAGAGAAGCCCTACACTTGCCTCGAGTGCAACCGCCGCTTCCGCCAG
CGCACGGCCCTCGTCATCCACCAGCGCATCCACACGGGCGAGAAGCCCTACCCGTGCCCG
GACTGCGAGCGGCGCTTCTCCTCCTCCTCTCGCCTGGTCAGTCACCGGCGTGTGCACTCT
GGGGAGCGTCCCTATGCCTGCGAGCACTGTGAGGCCCGCTTCTCCCAGCGCAGCACGCTG
CTCCAGCACCAGCTCTTGCACACCGGAGAGAAGCCCTACCCCTGCCCAGACTGTGGGCGT
GCCTTCCGGCGGAGCGGCTCCCTGGCCATCCATCGCAGCACGCACACAGAGGAGAAGCTG
CACGCCTGCGACGACTGTGGTCGCCGCTTTGCCTACCCCTCACTGCTGGCCAGCCACCGG
CGCGTGCACTCGGGCGAGCGGCCCTATGCCTGCGACCTTTGCTCCAAGCGTTTTGCTCAG
TGGAGCCACCTGGCCCAGCACCAGCTGCTGCACACGGGGGAGAAGCCTTTCCCCTGCCTC
GAGTGTGGCCGGTGCTTCCGCCAGAGGTGGTCTCTGGCTGTCCACAAGTGTAGCCCCAAG
GCCCCAAACTGTAGCCCTAGATCTGCTATCGGGGGCTCCAGTCAGAGGGGCAACGCCCAT

Protein sequence predicted based on nucleotide sequence

MAPPSAPLPAQGPGKARPSRKRGRRPRALKFVDVAVYFSPEEWGCLRPAQRALYRDVMRE
TYGHLGALGCAGPKPALISWLERNTDDWEPAALDPQEYPRGLTVQRKSRTRKKNGEKEVF
PPKEAPRKGKRGRRPSKPRLIPRQTSGGPICPDCGCTFPDHQALESHKCAQNLKKPYPCP
DCGRRFSYPSLLVSHRRAHSGECPYVCDQCGKRFSQRKNLSQHQVIHTGEKPYHCPDCGR
CFRRSRSLANHRTTHTGEKPHQCPSCGRRFAYPSLLAIHQRTHTGEKPYTCLECNRRFRQ
RTALVIHQRIHTGEKPYPCPDCERRFSSSSRLVSHRRVHSGERPYACEHCEARFSQRSTL
LQHQLLHTGEKPYPCPDCGRAFRRSGSLAIHRSTHTEEKLHACDDCGRRFAYPSLLASHR
RVHSGERPYACDLCSKRFAQWSHLAQHQLLHTGEKPFPCLECGRCFRQRWSLAVHKCSPK
APNCSPRSAIGGSSQRGNAH

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF689

Symbol Nomenclature :

ZNF689

SYNONYM :

TIPUH1

Designation :

transcription-involved protein upregulated in HCC 1

Full Nomenclature :

zinc finger protein 689

GENEID :

115509

HGNC :

25173

Vega :

OTTHUMG00000132415

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00512536      ATGGCGCCACCTTCGGCTCCGCTCCCTGCGCAGGGACCAGGAAAGGCCAGACCCAGTCGG
NM_138447.2       ATGGCGCCACCTTCGGCTCCGCTCCCTGCGCAGGGACCAGGAAAGGCCAGACCCAGTCGG
                  ************************************************************

HsCD00512536      AAAAGGGGCAGGAGGCCGAGGGCTCTGAAGTTCGTGGACGTGGCCGTGTACTTCTCCCCG
NM_138447.2       AAAAGGGGCAGGAGGCCGAGGGCTCTGAAGTTCGTGGACGTGGCCGTGTACTTCTCCCCG
                  ************************************************************

HsCD00512536      GAGGAGTGGGGCTGCCTGCGGCCCGCGCAGAGGGCCCTGTACCGGGACGTGATGCGGGAG
NM_138447.2       GAGGAGTGGGGCTGCCTGCGGCCCGCGCAGAGGGCCCTGTACCGGGACGTGATGCGGGAG
                  ************************************************************

HsCD00512536      ACCTACGGTCACCTGGGCGCGCTCGGGTGCGCAGGTCCCAAACCAGCCCTCATCTCCTGG
NM_138447.2       ACCTACGGTCACCTGGGCGCGCTCGGGTGCGCAGGTCCCAAACCAGCCCTCATCTCCTGG
                  ************************************************************

HsCD00512536      TTGGAACGAAACACCGATGACTGGGAACCGGCTGCTCTAGATCCGCAGGAGTACCCGAGA
NM_138447.2       TTGGAACGAAACACCGATGACTGGGAACCGGCTGCTCTAGATCCGCAGGAGTACCCGAGA
                  ************************************************************

HsCD00512536      GGGCTAACAGTCCAGAGAAAAAGCAGAACCAGAAAGAAGAATGGGGAGAAGGAAGTATTC
NM_138447.2       GGGCTAACAGTCCAGAGAAAAAGCAGAACCAGAAAGAAGAATGGGGAGAAGGAAGTATTC
                  ************************************************************

HsCD00512536      CCGCCTAAGGAGGCACCCCGAAAGGGGAAGCGAGGCCGGAGGCCCAGCAAACCCCGACTG
NM_138447.2       CCGCCTAAGGAGGCACCCCGAAAGGGGAAGCGAGGCCGGAGGCCCAGCAAACCCCGACTG
                  ************************************************************

HsCD00512536      ATTCCTAGGCAGACGTCCGGGGGCCCCATCTGCCCTGACTGCGGCTGTACCTTCCCTGAT
NM_138447.2       ATTCCTAGGCAGACGTCCGGGGGCCCCATCTGCCCTGACTGCGGCTGTACCTTCCCTGAT
                  ************************************************************

HsCD00512536      CATCAGGCCCTGGAGAGCCACAAGTGCGCCCAGAATCTAAAAAAGCCTTACCCTTGCCCA
NM_138447.2       CATCAGGCCCTGGAGAGCCACAAGTGCGCCCAGAATCTAAAAAAGCCTTACCCTTGCCCA
                  ************************************************************

HsCD00512536      GACTGTGGGCGCCGCTTTTCCTATCCATCCCTGCTGGTCAGTCACCGGCGGGCACACTCC
NM_138447.2       GACTGTGGGCGCCGCTTTTCCTATCCATCCCTGCTGGTCAGTCACCGGCGGGCACACTCC
                  ************************************************************

HsCD00512536      GGCGAGTGCCCCTATGTTTGTGACCAGTGTGGCAAACGTTTCTCCCAGCGCAAGAACCTC
NM_138447.2       GGCGAGTGCCCCTATGTTTGTGACCAGTGTGGCAAACGTTTCTCCCAGCGCAAGAACCTC
                  ************************************************************

HsCD00512536      TCCCAGCACCAGGTCATCCATACAGGGGAGAAGCCCTATCACTGCCCTGACTGTGGTCGC
NM_138447.2       TCCCAGCACCAGGTCATCCATACAGGGGAGAAGCCCTATCACTGCCCTGACTGTGGTCGC
                  ************************************************************

HsCD00512536      TGCTTCCGGAGGAGCCGGTCCTTGGCCAATCACCGGACCACACACACAGGTGAAAAACCC
NM_138447.2       TGCTTCCGGAGGAGCCGGTCCTTGGCCAATCACCGGACCACACACACAGGTGAAAAACCC
                  ************************************************************

HsCD00512536      CACCAGTGCCCTAGCTGTGGACGTCGCTTCGCCTACCCCTCCCTGCTAGCCATCCACCAG
NM_138447.2       CACCAGTGCCCTAGCTGTGGACGTCGCTTCGCCTACCCCTCCCTGCTAGCCATCCACCAG
                  ************************************************************

HsCD00512536      CGTACACACACGGGAGAGAAGCCCTACACTTGCCTCGAGTGCAACCGCCGCTTCCGCCAG
NM_138447.2       CGTACACACACGGGAGAGAAGCCCTACACTTGCCTCGAGTGCAACCGCCGCTTCCGCCAG
                  ************************************************************

HsCD00512536      CGCACGGCCCTCGTCATCCACCAGCGCATCCACACGGGCGAGAAGCCCTACCCGTGCCCG
NM_138447.2       CGCACGGCCCTCGTCATCCACCAGCGCATCCACACGGGCGAGAAGCCCTACCCGTGCCCG
                  ************************************************************

HsCD00512536      GACTGCGAGCGGCGCTTCTCCTCCTCCTCTCGCCTGGTCAGTCACCGGCGTGTGCACTCT
NM_138447.2       GACTGCGAGCGGCGCTTCTCCTCCTCCTCTCGCCTGGTCAGTCACCGGCGTGTGCACTCT
                  ************************************************************

HsCD00512536      GGGGAGCGTCCCTATGCCTGCGAGCACTGTGAGGCCCGCTTCTCCCAGCGCAGCACGCTG
NM_138447.2       GGGGAGCGTCCCTATGCCTGCGAGCACTGTGAGGCCCGCTTCTCCCAGCGCAGCACGCTG
                  ************************************************************

HsCD00512536      CTCCAGCACCAGCTCTTGCACACCGGAGAGAAGCCCTACCCCTGCCCAGACTGTGGGCGT
NM_138447.2       CTCCAGCACCAGCTCTTGCACACCGGAGAGAAGCCCTACCCCTGCCCAGACTGTGGGCGT
                  ************************************************************

HsCD00512536      GCCTTCCGGCGGAGCGGCTCCCTGGCCATCCATCGCAGCACGCACACAGAGGAGAAGCTG
NM_138447.2       GCCTTCCGGCGGAGCGGCTCCCTGGCCATCCATCGCAGCACGCACACAGAGGAGAAGCTG
                  ************************************************************

HsCD00512536      CACGCCTGCGACGACTGTGGTCGCCGCTTTGCCTACCCCTCACTGCTGGCCAGCCACCGG
NM_138447.2       CACGCCTGCGACGACTGTGGTCGCCGCTTTGCCTACCCCTCACTGCTGGCCAGCCACCGG
                  ************************************************************

HsCD00512536      CGCGTGCACTCGGGCGAGCGGCCCTATGCCTGCGACCTTTGCTCCAAGCGTTTTGCTCAG
NM_138447.2       CGCGTGCACTCGGGCGAGCGGCCCTATGCCTGCGACCTTTGCTCCAAGCGTTTTGCTCAG
                  ************************************************************

HsCD00512536      TGGAGCCACCTGGCCCAGCACCAGCTGCTGCACACGGGGGAGAAGCCTTTCCCCTGCCTC
NM_138447.2       TGGAGCCACCTGGCCCAGCACCAGCTGCTGCACACGGGGGAGAAGCCTTTCCCCTGCCTC
                  ************************************************************

HsCD00512536      GAGTGTGGCCGGTGCTTCCGCCAGAGGTGGTCTCTGGCTGTCCACAAGTGTAGCCCCAAG
NM_138447.2       GAGTGTGGCCGGTGCTTCCGCCAGAGGTGGTCTCTGGCTGTCCACAAGTGTAGCCCCAAG
                  ************************************************************

HsCD00512536      GCCCCAAACTGTAGCCCTAGATCTGCTATCGGGGGCTCCAGTCAGAGGGGCAACGCCCAT
NM_138447.2       GCCCCAAACTGTAGCCCTAGATCTGCTATCGGGGGCTCCAGTCAGAGGGGCAACGCCCAT
                  ************************************************************

HsCD00512536      ---
NM_138447.2       TAG