Gene:
Gene Symbol:
EPC1
Gene Name:
enhancer of polycomb homolog 1 (Drosophila)
Original Clone ID:
ccsbBroadEn_12691
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC036529.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00512556
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGTAAACTGTCGTTTCGGGCGCGGGCGCTAGACGCCTCGAAGCCGCTGCCGGTTTTC
CGCTGTGAGGATCTGCCCGACCTGCACGAATACGCCTCGATAAACAGGGCCGTGCCGCAG
ATGCCCACCGGAATGGAGAAGGAAGAGGAGTCGGAACATCATCTTCAGCGGGCTATTTCA
GCACAGCAGGTGTATGGCGAGAAGAGGGATAATATGGTTATACCGGTCCCAGAGGCAGAA
AGTAATATTGCTTACTATGAGTCTATATATCCTGGGGAATTTAAGATGCCAAAGCAGCTC
ATTCACATACAGCCTTTTAGTTTGGATGCTGAACAGCCTGATTATGATTTGGATTCTGAA
GATGAAGTATTTGTGAATAAACTGAAAAAGAAAATGGACATCTGCCCATTGCAATTTGAG
GAGATGATTGACCGCCTAGAAAAAGGCAGTGGTCAGCAGCCAGTCAGTCTGCAGGAAGCC
AAACTACTGCTAAAAGAAGATGATGAACTAATTAGAGAAGTTTATGAATATTGGATTAAA
AAGAGAAAAAACTGTCGAGGGCCATCTCTTATTCCATCAGTAAAACAAGAGAAGCGAGAT
GGTTCCAGCACAAATGATCCTTATGTGGCTTTTAGAAGGCGTACTGAAAAAATGCAGACT
CGAAAAAATCGCAAAAATGATGAAGCCTCTTACGAAAAAATGCTTAAGCTGCGACGAGAT
CTAAGTCGAGCTGTTACTATTCTAGAGATGATAAAAAGAAGAGAAAAAAGTAAAAGAGAG
CTATTGCACTTAACACTGGAAATTATGGAAAAGAGGTATAATTTGGGCGACTACAATGGA
GAGATCATGTCTGAGGTTATGGCACAGAGACAGCCAATGAAACCTACTTATGCCATCCCC
ATCATCCCTATTACTAATAGCAGTCAATTTAAACACCAGGAAGCAATGGATGTGAAGGAG
TTCAAAGTTAATAAGCAAGATAAAGCCGATCTTATCCGACCGAAACGGAAATATGAAAAG
AAGCCCAAAGTCTTACCATCGTCTGCCGCTGCTACTCCCCAACAGACGAGTCCTGCTGCA
CTGCCAGTCTTCAATGCTAAAGATCTGAATCAGTATGACTTTCCCAGCTCAGACGAAGAA
CCTCTCTCCCAGGTTTTGTCTGGCTCTTCGGAAGCTGAGGAAGACAATGATCCTGATGGT
CCTTTTGCTTTCCGTAGGAAAGCAGGCTGTCAGTACTATGCTCCTCACTTAGACCAAACT
GGCAACTGGCCTTGGACTAGTCCTAAAGATGGAGGATTAGGGGATGTGCGATATAGATAC
TGCTTAACTACTCTCACCGTACCCCAAAGGTGTATTGGATTTGCACGAAGACGGGTTGGG
CGCGGTGGAAGGGTCTTACTGGACAGAGCTCATTCAGACTATGACAGTGTGTTTCACCAT
CTGGATTTGGAAATGCTTTCCTCACCACAACATTCTCCAGTCAATCAGTTTGCCAATACC
TCAGAAACAAATACCTCGGACAAATCTTTCTCTAAAGACCTCAGTCAGATACTAGTCAAT
ATCAAATCATGTAGATGGCGGCATTTTAGGCCTCGGACACCATCCCTACATGACAGTGAC
AATGATGAACTCTCCTGTAGAAAATTATATAGGAGTATAAACCGAACAGGAACAGCGCAA
CCTGGGACCCAGACATGCAGTACCTCTACGCAAAGTAAAAGTAGCAGTGGTTCAGCACAC
TTTGCATTTACAGCCGAACAATACCAGCAACATCAACAGCAACTGGCACTCATGCAGAAA
CAGCAGCTTGCACAAATTCAGCAACAGCAAGCAAATAGTAATTCCTCCACCAACACATCA
CAGGGTTTTGTTTCTAAGACTTTGGATTCTGCTAGTGCACAGTTTGCTGCTTCTGCTTTG
GTGACATCAGAACAACTGATGGGATTCAAGATGAAGGATGATGTGGTGCTTGGAATCGGG
GTGAATGGCGTCCTTCCAGCCTCAGGAGTATACAAGGGCTTACACCTCAGTAGTACTACA
CCAACAGCACTTGTACATACAAGTCCATCAACGGCAGGTTCAGCTTTGTTACAGCCTTCA
AATATTACACAGACTTCAAGTTCCCACAGTGCACTGAGTCATCAAGTAACTGCTGCCAAT
TCTGCAACAACTCAGGTTCTGATTGGGAACAACATTCGATTAACTGTACCTTCATCAGTT
GCCACTGTAAACTCTATTGCCCCAATAAATGCACGACATATACCTAGGACTTTAAGTGTT
GTTCCATCATCTGCCTTAAAGCTGGCCGCTGCAGCAAACTGTCAAGTTTCCAAGGTCCCA
TCTTCATCCTCTGTAGATTCAGTTCCAAGGGAAAATCATGAATCAGAAAAGCCAGCACTG
AACAACATAGCAGACAACACAGTAGCGATGGAGGTGACG
Protein sequence predicted based on nucleotide sequence
MSKLSFRARALDASKPLPVFRCEDLPDLHEYASINRAVPQMPTGMEKEEESEHHLQRAIS AQQVYGEKRDNMVIPVPEAESNIAYYESIYPGEFKMPKQLIHIQPFSLDAEQPDYDLDSE DEVFVNKLKKKMDICPLQFEEMIDRLEKGSGQQPVSLQEAKLLLKEDDELIREVYEYWIK KRKNCRGPSLIPSVKQEKRDGSSTNDPYVAFRRRTEKMQTRKNRKNDEASYEKMLKLRRD LSRAVTILEMIKRREKSKRELLHLTLEIMEKRYNLGDYNGEIMSEVMAQRQPMKPTYAIP IIPITNSSQFKHQEAMDVKEFKVNKQDKADLIRPKRKYEKKPKVLPSSAAATPQQTSPAA LPVFNAKDLNQYDFPSSDEEPLSQVLSGSSEAEEDNDPDGPFAFRRKAGCQYYAPHLDQT GNWPWTSPKDGGLGDVRYRYCLTTLTVPQRCIGFARRRVGRGGRVLLDRAHSDYDSVFHH LDLEMLSSPQHSPVNQFANTSETNTSDKSFSKDLSQILVNIKSCRWRHFRPRTPSLHDSD NDELSCRKLYRSINRTGTAQPGTQTCSTSTQSKSSSGSAHFAFTAEQYQQHQQQLALMQK QQLAQIQQQQANSNSSTNTSQGFVSKTLDSASAQFAASALVTSEQLMGFKMKDDVVLGIG VNGVLPASGVYKGLHLSSTTPTALVHTSPSTAGSALLQPSNITQTSSSHSALSHQVTAAN SATTQVLIGNNIRLTVPSSVATVNSIAPINARHIPRTLSVVPSSALKLAAAANCQVSKVP SSSSVDSVPRENHESEKPALNNIADNTVAMEVT
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
