DNASU Plasmid | NPFFR1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: NPFFR1

Gene Name: neuropeptide FF receptor 1

Original Clone ID:

ccsbBroadEn_08834

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC131580.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512792

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1291nts

Open reading frame: 1 to 1291

Reference Sequence Alignment

ATGGAGGGGGAGCCCTCCCAGCCTCCCAACAGCAGTTGGCCCCTAAGTCAGAATGGGACT
AACACTGAGGCCACCCCGGCTACAAACCTCACCTTCTCCTCCTACTATCAGCACACCTCC
CCTGTGGCGGCCATGTTCATTGTGGCCTATGCGCTCATCTTCCTGCTCTGCATGGTGGGC
AACACCCTGGTCTGTTTCATCGTGCTCAAGAACCGGCACATGCATACTGTCACCAACATG
TTCATCCTCAACCTGGCTGTCAGTGACCTGCTGGTGGGCATCTTCTGCATGCCCACCACC
CTTGTGGACAACCTCATCACTGGGTGGCCCTTCGACAATGCCACATGCAAGATGAGCGGC
TTGGTGCAGGGCATGTCTGTGTCGGCTTCCGTTTTCACACTGGTGGCCATTGCTGTGGAA
AGGTTCCGCTGCATCGTGCACCCTTTCCGCGAGAAGCTGACCCTGCGGAAGGCGCTCGTC
ACCATCGCCGTCATCTGGGCCCTGGCGCTGCTCATCATGTGTCCCTCGGCCGTCACGCTG
ACCGTCACCCGTGAGGAGCACCACTTCATGGTGGACGCCCGCAACCGCTCCTACCCTCTC
TACTCCTGCTGGGAGGCCTGGCCCGAGAAGGGCATGCGCAGGGTCTACACCACTGTGCTC
TTCTCGCACATCTACCTGGCGCCGCTGGCGCTCATCGTGGTCATGTACGCCCGCATCGCG
CGCAAGCTCTGCCAGGCCCCGGGCCCGGCCCCCGGGGGCGAGGAGGCTGCGGACCCGCGA
GCATCGCGGCGCAGAGCGCGCGTGGTGCACATGCTGGTCATGGTGGCGCTGTTCTTCACG
CTGTCCTGGCTGCCGCTCTGGGCGCTGCTGCTGCTCATCGACTACGGGCAGCTCAGCGCG
CCGCAGCTGCACCTGGTCACCGTCTACGCCTTCCCCTTCGCGCACTGGCTGGCCTTCTTC
AACAGCAGCGCCAACCCCATCATCTACGGCTACTTCAACGAGAACTTCCGCCGCGGCTTC
CAGGCCGCCTTCCGCGCCCGCCTCTGCCCGCGCCCGTCGGGGAGCCACAAGGAGGCCTAC
TCCGAGCGGCCCGGCGGGCTTCTGCACAGGCGGGTCTTCGTGGTGGTGCGGCCCAGCGAC
TCCGGGCTGCCCTCTGAGTCGGGCCCTAGCAGTGGGGCCCCCAGGCCCGGCCGCCTCCCG
CTGCGGAATGGGCGGGTGGCTCACCACGGCTTGCCCAGGGAAGGGCCTGGCTGCTCCCAC
CTGCCCCTCACCATTCCAGCCTGGGATATC

Protein sequence predicted based on nucleotide sequence

MEGEPSQPPNSSWPLSQNGTNTEATPATNLTFSSYYQHTSPVAAMFIVAYALIFLLCMVG
NTLVCFIVLKNRHMHTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSG
LVQGMSVSASVFTLVAIAVERFRCIVHPFREKLTLRKALVTIAVIWALALLIMCPSAVTL
TVTREEHHFMVDARNRSYPLYSCWEAWPEKGMRRVYTTVLFSHIYLAPLALIVVMYARIA
RKLCQAPGPAPGGEEAADPRASRRRARVVHMLVMVALFFTLSWLPLWALLLLIDYGQLSA
PQLHLVTVYAFPFAHWLAFFNSSANPIIYGYFNENFRRGFQAAFRARLCPRPSGSHKEAY
SERPGGLLHRRVFVVVRPSDSGLPSESGPSSGAPRPGRLPLRNGRVAHHGLPREGPGCSH
LPLTIPAWDI

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

NPFFR1

Symbol Nomenclature :

NPFFR1

SYNONYM :

GPR147; NPFF1; NPFF1R1; OT7T022

Designation :

G protein-coupled receptor 147|G-protein coupled receptor 147|RFamide-related peptide receptor OT7T022|neuropeptide FF 1

Full Nomenclature :

neuropeptide FF receptor 1

GENEID :

64106

Locus Tag :

RP11-203J14.1

HGNC :

17425

MIM :

607448

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00512792      ATGGAGGGGGAGCCCTCCCAGCCTCCCAACAGCAGTTGGCCCCTAAGTCAGAATGGGACT
NM_022146.4       ATGGAGGGGGAGCCCTCCCAGCCTCCCAACAGCAGTTGGCCCCTAAGTCAGAATGGGACT
                  ************************************************************

HsCD00512792      AACACTGAGGCCACCCCGGCTACAAACCTCACCTTCTCCTCCTACTATCAGCACACCTCC
NM_022146.4       AACACTGAGGCCACCCCGGCTACAAACCTCACCTTCTCCTCCTACTATCAGCACACCTCC
                  ************************************************************

HsCD00512792      CCTGTGGCGGCCATGTTCATTGTGGCCTATGCGCTCATCTTCCTGCTCTGCATGGTGGGC
NM_022146.4       CCTGTGGCGGCCATGTTCATTGTGGCCTATGCGCTCATCTTCCTGCTCTGCATGGTGGGC
                  ************************************************************

HsCD00512792      AACACCCTGGTCTGTTTCATCGTGCTCAAGAACCGGCACATGCATACTGTCACCAACATG
NM_022146.4       AACACCCTGGTCTGTTTCATCGTGCTCAAGAACCGGCACATGCATACTGTCACCAACATG
                  ************************************************************

HsCD00512792      TTCATCCTCAACCTGGCTGTCAGTGACCTGCTGGTGGGCATCTTCTGCATGCCCACCACC
NM_022146.4       TTCATCCTCAACCTGGCTGTCAGTGACCTGCTGGTGGGCATCTTCTGCATGCCCACCACC
                  ************************************************************

HsCD00512792      CTTGTGGACAACCTCATCACTGGGTGGCCCTTCGACAATGCCACATGCAAGATGAGCGGC
NM_022146.4       CTTGTGGACAACCTCATCACTGGGTGGCCCTTCGACAATGCCACATGCAAGATGAGCGGC
                  ************************************************************

HsCD00512792      TTGGTGCAGGGCATGTCTGTGTCGGCTTCCGTTTTCACACTGGTGGCCATTGCTGTGGAA
NM_022146.4       TTGGTGCAGGGCATGTCTGTGTCGGCTTCCGTTTTCACACTGGTGGCCATTGCTGTGGAA
                  ************************************************************

HsCD00512792      AGGTTCCGCTGCATCGTGCACCCTTTCCGCGAGAAGCTGACCCTGCGGAAGGCGCTCGTC
NM_022146.4       AGGTTCCGCTGCATCGTGCACCCTTTCCGCGAGAAGCTGACCCTGCGGAAGGCGCTCGTC
                  ************************************************************

HsCD00512792      ACCATCGCCGTCATCTGGGCCCTGGCGCTGCTCATCATGTGTCCCTCGGCCGTCACGCTG
NM_022146.4       ACCATCGCCGTCATCTGGGCCCTGGCGCTGCTCATCATGTGTCCCTCGGCCGTCACGCTG
                  ************************************************************

HsCD00512792      ACCGTCACCCGTGAGGAGCACCACTTCATGGTGGACGCCCGCAACCGCTCCTACCCTCTC
NM_022146.4       ACCGTCACCCGTGAGGAGCACCACTTCATGGTGGACGCCCGCAACCGCTCCTACCCGCTC
                  ******************************************************** ***

HsCD00512792      TACTCCTGCTGGGAGGCCTGGCCCGAGAAGGGCATGCGCAGGGTCTACACCACTGTGCTC
NM_022146.4       TACTCCTGCTGGGAGGCCTGGCCCGAGAAGGGCATGCGCAGGGTCTACACCACTGTGCTC
                  ************************************************************

HsCD00512792      TTCTCGCACATCTACCTGGCGCCGCTGGCGCTCATCGTGGTCATGTACGCCCGCATCGCG
NM_022146.4       TTCTCGCACATCTACCTGGCGCCGCTGGCGCTCATCGTGGTCATGTACGCCCGCATCGCG
                  ************************************************************

HsCD00512792      CGCAAGCTCTGCCAGGCCCCGGGCCCGGCCCCCGGGGGCGAGGAGGCTGCGGACCCGCGA
NM_022146.4       CGCAAGCTCTGCCAGGCCCCGGGCCCGGCCCCCGGGGGCGAGGAGGCTGCGGACCCGCGA
                  ************************************************************

HsCD00512792      GCATCGCGGCGCAGAGCGCGCGTGGTGCACATGCTGGTCATGGTGGCGCTGTTCTTCACG
NM_022146.4       GCATCGCGGCGCAGAGCGCGCGTGGTGCACATGCTGGTCATGGTGGCGCTGTTCTTCACG
                  ************************************************************

HsCD00512792      CTGTCCTGGCTGCCGCTCTGGGCGCTGCTGCTGCTCATCGACTACGGGCAGCTCAGCGCG
NM_022146.4       CTGTCCTGGCTGCCGCTCTGGGCGCTGCTGCTGCTCATCGACTACGGGCAGCTCAGCGCG
                  ************************************************************

HsCD00512792      CCGCAGCTGCACCTGGTCACCGTCTACGCCTTCCCCTTCGCGCACTGGCTGGCCTTCTTC
NM_022146.4       CCGCAGCTGCACCTGGTCACCGTCTACGCCTTCCCCTTCGCGCACTGGCTGGCCTTCTTC
                  ************************************************************

HsCD00512792      AACAGCAGCGCCAACCCCATCATCTACGGCTACTTCAACGAGAACTTCCGCCGCGGCTTC
NM_022146.4       AACAGCAGCGCCAACCCCATCATCTACGGCTACTTCAACGAGAACTTCCGCCGCGGCTTC
                  ************************************************************

HsCD00512792      CAGGCCGCCTTCCGCGCCCGCCTCTGCCCGCGCCCGTCGGGGAGCCACAAGGAGGCCTAC
NM_022146.4       CAGGCCGCCTTCCGCGCCCGCCTCTGCCCGCGCCCGTCGGGGAGCCACAAGGAGGCCTAC
                  ************************************************************

HsCD00512792      TCCGAGCGGCCCGGCGGGCTTCTGCACAGGCGGGTCTTCGTGGTGGTGCGGCCCAGCGAC
NM_022146.4       TCCGAGCGGCCCGGCGGGCTTCTGCACAGGCGGGTCTTCGTGGTGGTGCGGCCCAGCGAC
                  ************************************************************

HsCD00512792      TCCGGGCTGCCCTCTGAGTCGGGCCCTAGCAGTGGGGCCCCCAGGCCCGGCCGCCTCCCG
NM_022146.4       TCCGGGCTGCCCTCTGAGTCGGGCCCTAGCAGTGGGGCCCCCAGGCCCGGCCGCCTCCCG
                  ************************************************************

HsCD00512792      CTGCGGAATGGGCGGGTGGCTCACCACGGCTTGCCCAGGGAAGGGCCTGGCTGCTCCCAC
NM_022146.4       CTGCGGAATGGGCGGGTGGCTCACCACGGCTTGCCCAGGGAAGGGCCTGGCTGCTCCCAC
                  ************************************************************

HsCD00512792      CTGCCCCTCACCATTCCAGCCTGGGATATC---
NM_022146.4       CTGCCCCTCACCATTCCAGCCTGGGATATCTGA
                  ******************************

Pathways

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

G alpha (q) signalling events

Reactome :

Gastrin-CREB signalling pathway via PKC and MAPK

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

Orexin and neuropeptides FF and QRFP bind to their respective receptors

Reactome :

Peptide ligand-binding receptors

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

WikiPathway :

GPCRs, Class A Rhodopsin-like

Protein

HPRD :

12120

Clone

Cloning Information :

669407

Original Clone ID :

ccsbBroadEn_08834

Organism

Species Specific ID:

64106

Genome

Chromosome :

Map Location :

10q21-q22

Reagents

Labome :

NPFFR1-antibody