DNASU Plasmid | PRR5-ARHGAP8 (H. sapiens )<br>In pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ARHGAP8

Gene Name: Rho GTPase activating protein 8

Original Clone ID:

ccsbBroadEn_13712

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC132755.1,BC132757.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512892

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1408nts

Open reading frame: 1 to 1408

Reference Sequence Alignment

ATGGTGATCCTTCGGGACAAGATTCGCTTCTATGAGGAGCTGCAGAGAGACAAGGCGGCG
GCGGCTGCTGTGCTGGGTGCAGTGAGGAAGAGGCCCTCGGTGGTGCCCATGGCTGGCCAG
GATCCTGCGCTGAGCACGAGTCACCCGTTCTACGACGTGGCCAGACATGGCATTCTGCAG
GTGGCAGGGGATGACCGCTTTGGAAGACGTGTTGTCACGTTCAGCTGCTGCCGGATGCCG
CCCTCCCACGAGCTGGACCACCAGCGGCTGCTGGAGTATTTGAAGTACACACTGGACCAA
TACGTTGAGAACGATTATACCATCGTCTATTTCCACTACGGGCTGAACAGCCGGAACAAG
CCTTCCCTGGGCTGGCTCCAGAGCGCATACAAGGAGTTCGATAGGAAGTACAAGAAGAAC
TTGAAGGCCCTCTACGTGGTGCACCCCACCAGCTTCATCAAGGTCCTGTGGAACATCTTG
AAGCCCCTCATCAGTCACAAGTTTGGGAAGAAAGTCATCTATTTCAACTACCTGAGTGAG
CTCCACGAACACCTTAAATACGACCAGCTGGTCATCCCTCCCGAAGTTTTGCGGTACGAT
GAGAAGCTCCAGAGCCTGCACGAGGGCCGGACGCCGCCTCCCACCAAGACACCACCGCCG
CGGCCCCCGCTGCCCACACAGCAGTTTGGCGTCAGTCTGCAATACCTCAAAGACAAAAAT
CAAGGCGAACTCATCCCCCCTGTGCTGAGGTTCACAGTGACGTACCTGAGAGAGAAAGGC
CTGCGCACCGAGGGCCTGTTCCGGAGATCCGCCAGCGTGCAGACCGTCCGCGAGATCCAG
AGGCTCTACAACCAAGGGAAGCCCGTGAACTTTGACGACTACGGGGACATTCACATCCCT
GCCGTGATCCTGAAGACCTTCCTGCGAGAGCTGCCCCAGCCGCTTCTGACCTTCCAGGCC
TACGAGCAGATTCTCGGGATCACCTGTGTGGAGAGCAGCCTGCGTGTCACTGGCTGCCGC
CAGATCTTACGGAGCCTCCCAGAGCACAACTACGTCGTCCTCCGCTACCTCATGGGCTTC
CTGCATGCGGTGTCCCGGGAGAGCATCTTCAACAAAATGAACAGCTCTAACCTGGCCTGT
GTCTTCGGGCTGAATTTGATCTGGCCATCCCAGGGGGTCTCCTCCCTGAGTGCCCTTGTG
CCCCTGAACATGTTCACTGAACTGCTGATCGAGTACTATGAAAAGATCTTCAGCACCCCG
GAGGCACCTGGGGAGCACGGCCTGGCACCATGGGAACAGGGGAGCAGGGCAGCCCCTTTG
CAGGAGGCTGTGCCACGGACACAAGCCACGGGCCTCACCAAGCCTACCCTACCTCCGAGT
CCCCTGATGGCAGCCAGAAGACGTCTC

Protein sequence predicted based on nucleotide sequence

MVILRDKIRFYEELQRDKAAAAAVLGAVRKRPSVVPMAGQDPALSTSHPFYDVARHGILQ
VAGDDRFGRRVVTFSCCRMPPSHELDHQRLLEYLKYTLDQYVENDYTIVYFHYGLNSRNK
PSLGWLQSAYKEFDRKYKKNLKALYVVHPTSFIKVLWNILKPLISHKFGKKVIYFNYLSE
LHEHLKYDQLVIPPEVLRYDEKLQSLHEGRTPPPTKTPPPRPPLPTQQFGVSLQYLKDKN
QGELIPPVLRFTVTYLREKGLRTEGLFRRSASVQTVREIQRLYNQGKPVNFDDYGDIHIP
AVILKTFLRELPQPLLTFQAYEQILGITCVESSLRVTGCRQILRSLPEHNYVVLRYLMGF
LHAVSRESIFNKMNSSNLACVFGLNLIWPSQGVSSLSALVPLNMFTELLIEYYEKIFSTP
EAPGEHGLAPWEQGSRAAPLQEAVPRTQATGLTKPTLPPSPLMAARRRL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ARHGAP8

Symbol Nomenclature :

ARHGAP8

SYNONYM :

BPGAP1; PP610

Designation :

BCH domain-containing Cdc42GAP-like protein|BNIP-2 and Cdc42GAP homology domain-containing, proline-rich and Cdc42GAP-like protein subtype-1|rho GTPase-activating protein 8|rho-type GTPase-activating protein 8

Full Nomenclature :

Rho GTPase activating protein 8

GENEID :

23779

GenBank Accession :

HQ258425

HGNC :

677

MIM :

609405

Vega :

OTTHUMG00000030234

Target GenBank:

HQ258425

Reference Sequence Alignment

  

HsCD00512892        ------------------------------------------------------------
NM_001017526.1      ------------------------------------------------------------
NM_181334.4         ATGAGTTCGCCCAGCCTCAGTGACCTGGGCAAGAGAGAGCCGGCCGCCGCCGCGGACGAG
NM_181335.2         ------------------------------------------------------------
                                                                                

HsCD00512892        ------------------------------------------------------------
NM_001017526.1      ------------------------------------------------------------
NM_181334.4         CGGGGCACGCAGCAGCGCCGGGCCTGCGCCAACGCCACCTGGAACAGCATCCACAACGGG
NM_181335.2         ------------------------------------------------------------
                                                                                

HsCD00512892        ------------------------------------------------------------
NM_001017526.1      ------------------------------------------------------------
NM_181334.4         GTGATCGCCGTCTTCCAGCGCAAGGGGCTGCCCGACCAGGAGCTCTTCAGCCTCAACGAG
NM_181335.2         ------------------------------------------------------------
                                                                                

HsCD00512892        ------------------------------------------------------------
NM_001017526.1      ------------------------------------------------------------
NM_181334.4         GGCGTCCGGCAGCTGTTGAAGACAGAGCTGGGGTCCTTCTTCACGGAGTACCTGCAGAAC
NM_181335.2         ------------------------------------------------------------
                                                                                

HsCD00512892        ------------------ATGGTGATCCTTCGGGACAAGATTCGCTTCTATGAGGAGCTG
NM_001017526.1      ------------------------------------------------------------
NM_181334.4         CAGCTGCTGACAAAAGGCATGGTGATCCTTCGGGACAAGATTCGCTTCTATGAGGAGCTG
NM_181335.2         ------------------------------------------------------------
                                                                                

HsCD00512892        CAGAGAGACAAGGCGGCGGCGGCTGCTGTGCTGGGTGCAGTGAGGAAGAGGCCCTCGGTG
NM_001017526.1      ------------------------------------------------------------
NM_181334.4         CAGAGAGACAAGGCGGCGGCGGCTGCTGTGCTGGGTGCAGTGAGGAAGAGGCCCTCGGTG
NM_181335.2         ------------------------------------------------------------
                                                                                

HsCD00512892        GTGCCCATGGCTGGCCAGGATCCTGCGCTGAGCACGAGTCACCCGTTCTACGACGTGGCC
NM_001017526.1      ------ATGGCTGGCCAGGATCCTGCGCTGAGCACGAGTCACCCGTTCTACGACGTGGCC
NM_181334.4         GTGCCCATGGCTGGCCAGGATCCTGCGCTGAGCACGAGTCACCCGTTCTACGACGTGGCC
NM_181335.2         ------ATGGCTGGCCAGGATCCTGCGCTGAGCACGAGTCACCCGTTCTACGACGTGGCC
                          ******************************************************

HsCD00512892        AGACATGGCATTCTGCAGGTGGCAGGGGATGACCGCTTTGGAAGACGTGTTGTCACGTTC
NM_001017526.1      AGACATGGCATTCTGCAGGTGGCAGGGGATGACCGCTTTGGAAGACGTGTTGTCACGTTC
NM_181334.4         AGACATGGCATTCTGCAGGTGGCAGGGGATGACCGCTTTGGAAGACGTGTTGTCACGTTC
NM_181335.2         AGACATGGCATTCTGCAGGTGGCAGGGGATGACCGCTTTGGAAGACGTGTTGTCACGTTC
                    ************************************************************

HsCD00512892        AGCTGCTGCCGGATGCCGCCCTCCCACGAGCTGGACCACCAGCGGCTGCTGGAGTATTTG
NM_001017526.1      AGCTGCTGCCGGATGCCACCCTCCCACGAGCTGGACCACCAGCGGCTGCTGGAGTATTTG
NM_181334.4         AGCTGCTGCCGGATGCCACCCTCCCACGAGCTGGACCACCAGCGGCTGCTGGAGTATTTG
NM_181335.2         AGCTGCTGCCGGATGCCACCCTCCCACGAGCTGGACCACCAGCGGCTGCTGGAGTATTTG
                    *****************.******************************************

HsCD00512892        AAGTACACACTGGACCAATACGTTGAGAACGATTATACCATCGTCTATTTCCACTACGGG
NM_001017526.1      AAGTACACACTGGACCAATACGTTGAGAACGATTATACCATCGTCTATTTCCACTACGGG
NM_181334.4         AAGTACACACTGGACCAATACGTTGAGAACGATTATACCATCGTCTATTTCCACTACGGG
NM_181335.2         AAGTACACACTGGACCAATACGTTGAGAACGATTATACCATCGTCTATTTCCACTACGGG
                    ************************************************************

HsCD00512892        CTGAACAGCCGGAACAAGCCTTCCCTGGGCTGGCTCCAGAGCGCATACAAGGAGTTCGAT
NM_001017526.1      CTGAACAGCCGGAACAAGCCTTCCCTGGGCTGGCTCCAGAGCGCATACAAGGAGTTCGAT
NM_181334.4         CTGAACAGCCGGAACAAGCCTTCCCTGGGCTGGCTCCAGAGCGCATACAAGGAGTTCGAT
NM_181335.2         CTGAACAGCCGGAACAAGCCTTCCCTGGGCTGGCTCCAGAGCGCATACAAGGAGTTCGAT
                    ************************************************************

HsCD00512892        AGGAAG------------------------------------------------------
NM_001017526.1      AG----GAAAGACGGGGATCTCACTATGTGGCCCAGGCTGGTCTCGAACTCCAAGCTCAA
NM_181334.4         AGGAAG------------------------------------------------------
NM_181335.2         AG----GAAG--------------------------------------------------
                    **                                                          

HsCD00512892        -------------------------------------------TACAAGAAGAACTTGAA
NM_001017526.1      GCGATCCTCCCACCTCAGCCTCCCAAAGTACTGGGATTACAGGTACAAGAAGAACTTGAA
NM_181334.4         -------------------------------------------TACAAGAAGAACTTGAA
NM_181335.2         -------------------------------------------TACAAGAAGAACTTGAA
                                                               *****************

HsCD00512892        GGCCCTCTACGTGGTGCACCCCACCAGCTTCATCAAGGTCCTGTGGAACATCTTGAAGCC
NM_001017526.1      GGCCCTCTACGTGGTGCACCCCACCAGCTTCATCAAGGTCCTGTGGAACATCTTGAAGCC
NM_181334.4         GGCCCTCTACGTGGTGCACCCCACCAGCTTCATCAAGGTCCTGTGGAACATCTTGAAGCC
NM_181335.2         GGCCCTCTACGTGGTGCACCCCACCAGCTTCATCAAGGTCCTGTGGAACATCTTGAAGCC
                    ************************************************************

HsCD00512892        CCTCATCAGTCACAAGTTTGGGAAGAAAGTCATCTATTTCAACTACCTGAGTGAGCTCCA
NM_001017526.1      CCTCATCAGTCACAAGTTTGGGAAGAAAGTCATCTATTTCAACTACCTGAGTGAGCTCCA
NM_181334.4         CCTCATCAGTCACAAGTTTGGGAAGAAAGTCATCTATTTCAACTACCTGAGTGAGCTCCA
NM_181335.2         CCTCATCAGTCACAAGTTTGGGAAGAAAGTCATCTATTTCAACTACCTGAGTGAGCTCCA
                    ************************************************************

HsCD00512892        CGAACACCTTAAATACGACCAGCTGGTCATCCCTCCCGAAGTTTTGCGGTACGATGAGAA
NM_001017526.1      CGAACACCTTAAATACGACCAGCTGGTCATCCCTCCCGAAGTTTTGCGGTACGATGAGAA
NM_181334.4         CGAACACCTTAAATACGACCAGCTGGTCATCCCTCCCGAAGTTTTGCGGTACGATGAGAA
NM_181335.2         CGAACACCTTAAATACGACCAGCTGGTCATCCCTCCCGAAGTTTTGCGGTACGATGAGAA
                    ************************************************************

HsCD00512892        GCTCCAGAGCCTGCACGAGGGCCGGACGCCGCCTCCCACCAAGACACCACCGCCGCGGCC
NM_001017526.1      GCTCCAGAGCCTGCACGAGGGCCGGACGCCGCCTCCCACCAAGACACCACCGCCGCGGCC
NM_181334.4         GCTCCAGAGCCTGCACGAGGGCCGGACGCCGCCTCCCACCAAGACACCACCGCCGCGGCC
NM_181335.2         GCTCCAGAGCCTGCACGAGGGCCGGACGCCGCCTCCCACCAAGACACCACCGCCGCGGCC
                    ************************************************************

HsCD00512892        CCCGCTGCCCACACAGCAGTTTGGCGTCAGTCTGCAATACCTCAAAGACAAAAATCAAGG
NM_001017526.1      CCCGCTGCCCACACAGCAGTTTGGCGTCAGTCTGCAATACCTCAAAGACAAAAATCAAGG
NM_181334.4         CCCGCTGCCCACACAGCAGTTTGGCGTCAGTCTGCAATACCTCAAAGACAAAAATCAAGG
NM_181335.2         CCCGCTGCCCACACAGCAGTTTGGCGTCAGTCTGCAATACCTCAAAGACAAAAATCAAGG
                    ************************************************************

HsCD00512892        CGAACTCATCCCCCCTGTGCTGAGGTTCACAGTGACGTACCTGAGAGAGAAAGGCCTGCG
NM_001017526.1      CGAACTCATCCCCCCTGTGCTGAGGTTCACAGTGACGTACCTGAGAGAGAAAGGCCTGCG
NM_181334.4         CGAACTCATCCCCCCTGTGCTGAGGTTCACAGTGACGTACCTGAGAGAGAAAGGCCTGCG
NM_181335.2         CGAACTCATCCCCCCTGTGCTGAGGTTCACAGTGACGTACCTGAGAGAGAAAGGCCTGCG
                    ************************************************************

HsCD00512892        CACCGAGGGCCTGTTCCGGAGATCCGCCAGCGTGCAGACCGTCCGCGAGATCCAGAGGCT
NM_001017526.1      CACCGAGGGCCTGTTCCGGAGATCCGCCAGCGTGCAGACCGTCCGCGAGATCCAGAGGCT
NM_181334.4         CACCGAGGGCCTGTTCCGGAGATCCGCCAGCGTGCAGACCGTCCGCGAGATCCAGAGGCT
NM_181335.2         CACCGAGGGCCTGTTCCGGAGATCCGCCAGCGTGCAGACCGTCCGCGAGATCCAGAGGCT
                    ************************************************************

HsCD00512892        CTACAACCAAGGGAAGCCCGTGAACTTTGACGACTACGGGGACATTCACATCCCTGCCGT
NM_001017526.1      CTACAACCAAGGGAAGCCCGTGAACTTTGACGACTACGGGGACATTCACATCCCTGCCGT
NM_181334.4         CTACAACCAAGGGAAGCCCGTGAACTTTGACGACTACGGGGACATTCACATCCCTGCCGT
NM_181335.2         CTACAACCAAGGGAAGCCCGTGAACTTTGACGACTACGGGGACATTCACATCCCTGCCGT
                    ************************************************************

HsCD00512892        GATCCTGAAGACCTTCCTGCGAGAGCTGCCCCAGCCGCTTCTGACCTTCCAGGCCTACGA
NM_001017526.1      GATCCTGAAGACCTTCCTGCGAGAGCTGCCCCAGCCGCTTCTGACCTTCCAGGCCTACGA
NM_181334.4         GATCCTGAAGACCTTCCTGCGAGAGCTGCCCCAGCCGCTTCTGACCTTCCAGGCCTACGA
NM_181335.2         GATCCTGAAGACCTTCCTGCGAGAGCTGCCCCAGCCGCTTCTGACCTTCCAGGCCTACGA
                    ************************************************************

HsCD00512892        GCAGATTCTCGGGATCACCTGTGTGGAGAGCAGCCTGCGTGTCACTGGCTGCCGCCAGAT
NM_001017526.1      GCAGATTCTCGGGATCACCTGTGTGGAGAGCAGCCTGCGTGTCACTGGCTGCCGCCAGAT
NM_181334.4         GCAGATTCTCGGGATCACCTGTGTGGAGAGCAGCCTGCGTGTCACTGGCTGCCGCCAGAT
NM_181335.2         GCAGATTCTCGGGATCACCTGTGTGGAGAGCAGCCTGCGTGTCACTGGCTGCCGCCAGAT
                    ************************************************************

HsCD00512892        CTTACGGAGCCTCCCAGAGCACAACTACGTCGTCCTCCGCTACCTCATGGGCTTCCTGCA
NM_001017526.1      CTTACGGAGCCTCCCAGAGCACAACTACGTCGTCCTCCGCTACCTCATGGGCTTCCTGCA
NM_181334.4         CTTACGGAGCCTCCCAGAGCACAACTACGTCGTCCTCCGCTACCTCATGGGCTTCCTGCA
NM_181335.2         CTTACGGAGCCTCCCAGAGCACAACTACGTCGTCCTCCGCTACCTCATGGGCTTCCTGCA
                    ************************************************************

HsCD00512892        TGCGGTGTCCCGGGAGAGCATCTTCAACAAAATGAACAGCTCTAACCTGGCCTGTGTCTT
NM_001017526.1      TGCGGTGTCCCGGGAGAGCATCTTCAACAAAATGAACAGCTCTAACCTGGCCTGTGTCTT
NM_181334.4         TGCGGTGTCCCGGGAGAGCATCTTCAACAAAATGAACAGCTCTAACCTGGCCTGTGTCTT
NM_181335.2         TGCGGTGTCCCGGGAGAGCATCTTCAACAAAATGAACAGCTCTAACCTGGCCTGTGTCTT
                    ************************************************************

HsCD00512892        CGGGCTGAATTTGATCTGGCCATCCCAGGGGGTCTCCTCCCTGAGTGCCCTTGTGCCCCT
NM_001017526.1      CGGGCTGAATTTGATCTGGCCATCCCAGGGGGTCTCCTCCCTGAGTGCCCTTGTGCCCCT
NM_181334.4         CGGGCTGAATTTGATCTGGCCATCCCAGGGGGTCTCCTCCCTGAGTGCCCTTGTGCCCCT
NM_181335.2         CGGGCTGAATTTGATCTGGCCATCCCAGGGGGTCTCCTCCCTGAGTGCCCTTGTGCCCCT
                    ************************************************************

HsCD00512892        GAACATGTTCACTGAACTGCTGATCGAGTACTATGAAAAGATCTTCAGCACCCCGGAGGC
NM_001017526.1      GAACATGTTCACTGAACTGCTGATCGAGTACTATGAAAAGATCTTCAGCACCCCGGAGGC
NM_181334.4         GAACATGTTCACTGAACTGCTGATCGAGTACTATGAAAAGATCTTCAGCACCCCGGAGGC
NM_181335.2         GAACATGTTCACTGAACTGCTGATCGAGTACTATGAAAAGATCTTCAGCACCCCGGAGGC
                    ************************************************************

HsCD00512892        ACCTGGGGAGCACGGCCTGGCACCATGGGAACAGGGGAGCAGGGCAGCCCCTTTGCAGGA
NM_001017526.1      ACCTGGGGAGCACGGCCTGGCACCATGGGAACAGGGGAGCAGGGCAGCCCCTTTGCAGGA
NM_181334.4         ACCTGGGGAGCACGGCCTGGCACCATGGGAACAGGGGAGCAGGGCAGCCCCTTTGCAGGA
NM_181335.2         ACCTGGGGAGCACGGCCTGGCACCATGGGAACAGGGGAGCAGGGCAGCCCCTTTGCAGGA
                    ************************************************************

HsCD00512892        GGCTGTGCCACGGACACAAGCCACGGGCCTCACCAAGCCTACCCTACCTCCGAGTCCCCT
NM_001017526.1      GGCTGTGCCACGGACACAAGCCACGGGCCTCACCAAGCCTACCCTACCTCCGAGTCCCCT
NM_181334.4         GGCTGTGCCACGGACACAAGCCACGGGCCTCACCAAGCCTACCCTACCTCCGAGTCCCCT
NM_181335.2         GGCTGTGCCACGGACACAAGCCACGGGCCTCACCAAGCCTACCCTACCTCCGAGTCCCCT
                    ************************************************************

HsCD00512892        GATGGCAGCCAGAAGACGTCTC---
NM_001017526.1      GATGGCAGCCAGAAGACGTCTCTAG
NM_181334.4         GATGGCAGCCAGAAGACGTCTCTAG
NM_181335.2         GATGGCAGCCAGAAGACGTCTCTAG
                    **********************