DNASU Plasmid | C10orf2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: C10orf2

Gene Name: chromosome 10 open reading frame 2

Original Clone ID:

ccsbBroadEn_12305

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC033762.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512985

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1519nts

Open reading frame: 1 to 1519

Reference Sequence Alignment

ATGTTTGGCCTTACCAAGGTTACAGATGACACACTCAAGCGTTTCAGTGTGCGATATCTG
CGACCTGCTCGCAGTCTTGTCTTCCCTTGGTTCTCCCCTGGGGGCTCAGGATTACGAGGC
CTGAAGCTCCTAGAGGCTAAATGCCAGGGGGATGGAGTGAGCTACGAGGAAACCACTATT
CCCCGACCCAGCGCCTACCACAATCTGTTTGGATTACCACTGATTAGTCGTCGAGATGCT
GAGGTGGTACTGACGAGTCGTGAGCTTGACAGCCTGGCCTTGAACCAGTCCACGGGGCTG
CCTACCCTTACTCTACCCCGAGGAACGACCTGCTTACCCCCTGCCTTACTCCCTTACCTG
GAACAGTTCCGGCGGATTGTATTCTGGTTGGGGGATGACCTTCGGTCCTGGGAAGCCGCC
AAGTTGTTTGCACGAAAACTGAACCCCAAACGATGCTTCTTGGTGCGACCAGGAGACCAG
CAACCCCGTCCCCTGGAGGCCCTGAACGGAGGCTTCAATCTTTCTCGTATTCTTCGTACC
GCCCTGCCTGCCTGGCACAAGTCCATCGTATCTTTCCGGCAGCTTCGGGAGGAGGTGCTA
GGAGAACTGTCAAATGTGGAGCAAGCAGCTGGCCTCCGCTGGAGCCGCTTTCCAGACCTC
AATCGTATCTTGAAGGGACATCGAAAGGGCGAGCTGACGGTCTTCACAGGGCCAACAGGC
AGTGGAAAGACGACATTCATCAGTGAGTATGCCCTGGATTTGTGTTCCCAGGGGGTGAAC
ACACTGTGGGGTAGCTTCGAGATCAGCAATGTGAGACTAGCCCGGGTCATGCTGACACAG
TTTGCCGAGGGGCGGCTGGAAGATCAACTGGACAAATATGATCACTGGGCTGACCGCTTT
GAGGACCTGCCCCTCTATTTCATGACTTTCCATGGACAGCAAAGCATCAGGACTGTAATA
GATACAATGCAACATGCAGTCTACGTCTATGACATTTGTCATGTGATCATCGACAACCTG
CAGTTCATGATGGGTCACGAGCAGCTGTCCACAGACAGGATCGCAGCTCAAGACTACATC
ATCGGGGTCTTTCGGAAGTTTGCAACAGACAATAACTGCCATGTGACACTGGTCATTCAC
CCCCGGAAAGAGGATGATGACAAGGAACTGCAGACAGCGTCCATTTTTGGCTCAGCCAAA
GCAAGCCAGGAAGCAGACAATGTTCTGATCCTGCAGGACAGGAAGCTGGTAACCGGGCCA
GGGAAACGGTATCTGCAGGTGTCCAAGAACCGCTTTGATGGAGATGTAGGTGTCTTCCCG
CTTGAGTTCAACAAGAACTCCCTCACCTTCTCCATTCCACCAAAGAACAAGGCCCGGCTC
AAGAAGATCAAGGATGACACTGGACCAGTGGCCAAAAAGCCCTCTTCTGGCAAAAAGGGG
GCTACGACACAGAACTCTGAGATTTGCTCAGGCCAGGCCCCCACTCCCGACCAGCCAGAC
ACCTCCAAGCGTTCAAAG

Protein sequence predicted based on nucleotide sequence

MFGLTKVTDDTLKRFSVRYLRPARSLVFPWFSPGGSGLRGLKLLEAKCQGDGVSYEETTI
PRPSAYHNLFGLPLISRRDAEVVLTSRELDSLALNQSTGLPTLTLPRGTTCLPPALLPYL
EQFRRIVFWLGDDLRSWEAAKLFARKLNPKRCFLVRPGDQQPRPLEALNGGFNLSRILRT
ALPAWHKSIVSFRQLREEVLGELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTG
SGKTTFISEYALDLCSQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRF
EDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYI
IGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGP
GKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDTGPVAKKPSSGKKG
ATTQNSEICSGQAPTPDQPDTSKRSK

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

C10orf2

Symbol Nomenclature :

C10orf2

SYNONYM :

ATXN8; IOSCA; MTDPS7; PEO; PEO1; PEOA3; SANDO; SCA8; TWINL

Designation :

Full Nomenclature :

chromosome 10 open reading frame 2

GENEID :

56652

Locus Tag :

RP11-108L7.2

HGNC :

1160

MIM :

606075

Vega :

OTTHUMG00000018917

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00512985      ------------------------------------------------------------
NM_021830.4       ATGTGGGTCCTCCTCCGAAGTGGGTACCCCCTCCGTATCTTGTTACCCCTGCGTGGGGAG
                                                                              

HsCD00512985      ------------------------------------------------------------
NM_021830.4       TGGATGGGTCGGAGGGGCCTGCCCCGAAACTTGGCCCCAGGCCCTCCTCGCAGACGTTAC
                                                                              

HsCD00512985      ------------------------------------------------------------
NM_021830.4       AGGAAGGAGACTCTCCAAGCCTTGGATATGCCAGTGTTGCCTGTAACTGCAACTGAAATC
                                                                              

HsCD00512985      ------------------------------------------------------------
NM_021830.4       CGCCAGTATTTGCGGGGGCATGGGATCCCCTTCCAGGATGGTCACAGTTGCCTGCGGGCA
                                                                              

HsCD00512985      ------------------------------------------------------------
NM_021830.4       CTGAGCCCCTTTGCAGAGTCTTCACAGCTCAAAGGCCAGACTGGTGTTACCACTTCCTTC
                                                                              

HsCD00512985      ------------------------------------------------------------
NM_021830.4       AGCCTCTTCATTGACAAGACCACAGGCCACTTTCTCTGCATGACCAGCCTAGCAGAAGGG
                                                                              

HsCD00512985      ------------------------------------------------------------
NM_021830.4       AGCTGGGAAGACTTCCAGGCCAGCGTGGAGGGGCGAGGGGATGGGGCCAGGGAGGGGTTT
                                                                              

HsCD00512985      ------------------------------------------------------------
NM_021830.4       CTGCTTAGCAAGGCACCAGAATTTGAGGACAGCGAGGAGGTCCGGAGGATCTGGAACCGA
                                                                              

HsCD00512985      ------------------------------------------------------ATGTTT
NM_021830.4       GCAATACCTCTCTGGGAGCTGCCTGATCAGGAGGAGGTTCAGCTGGCTGATACAATGTTT
                                                                        ******

HsCD00512985      GGCCTTACCAAGGTTACAGATGACACACTCAAGCGTTTCAGTGTGCGATATCTGCGACCT
NM_021830.4       GGCCTTACCAAGGTTACAGATGACACACTCAAGCGTTTCAGTGTGCGATATCTGCGACCT
                  ************************************************************

HsCD00512985      GCTCGCAGTCTTGTCTTCCCTTGGTTCTCCCCTGGGGGCTCAGGATTACGAGGCCTGAAG
NM_021830.4       GCTCGCAGTCTTGTCTTCCCTTGGTTCTCCCCTGGGGGCTCAGGATTACGAGGCCTGAAG
                  ************************************************************

HsCD00512985      CTCCTAGAGGCTAAATGCCAGGGGGATGGAGTGAGCTACGAGGAAACCACTATTCCCCGA
NM_021830.4       CTCCTAGAGGCTAAATGCCAGGGGGATGGAGTGAGCTACGAGGAAACCACTATTCCCCGA
                  ************************************************************

HsCD00512985      CCCAGCGCCTACCACAATCTGTTTGGATTACCACTGATTAGTCGTCGAGATGCTGAGGTG
NM_021830.4       CCCAGCGCCTACCACAATCTGTTTGGATTACCACTGATTAGTCGTCGAGATGCTGAGGTG
                  ************************************************************

HsCD00512985      GTACTGACGAGTCGTGAGCTTGACAGCCTGGCCTTGAACCAGTCCACGGGGCTGCCTACC
NM_021830.4       GTACTGACGAGTCGTGAGCTTGACAGCCTGGCCTTGAACCAGTCCACGGGGCTGCCTACC
                  ************************************************************

HsCD00512985      CTTACTCTACCCCGAGGAACGACCTGCTTACCCCCTGCCTTACTCCCTTACCTGGAACAG
NM_021830.4       CTTACTCTACCCCGAGGAACGACCTGCTTACCCCCTGCCTTACTCCCTTACCTGGAACAG
                  ************************************************************

HsCD00512985      TTCCGGCGGATTGTATTCTGGTTGGGGGATGACCTTCGGTCCTGGGAAGCCGCCAAGTTG
NM_021830.4       TTCCGGCGGATTGTATTCTGGTTGGGGGATGACCTTCGGTCCTGGGAAGCCGCCAAGTTG
                  ************************************************************

HsCD00512985      TTTGCACGAAAACTGAACCCCAAACGATGCTTCTTGGTGCGACCAGGAGACCAGCAACCC
NM_021830.4       TTTGCACGAAAACTGAACCCCAAACGATGCTTCTTGGTGCGACCAGGAGACCAGCAACCC
                  ************************************************************

HsCD00512985      CGTCCCCTGGAGGCCCTGAACGGAGGCTTCAATCTTTCTCGTATTCTTCGTACCGCCCTG
NM_021830.4       CGTCCCCTGGAGGCCCTGAACGGAGGCTTCAATCTTTCTCGTATTCTTCGTACCGCCCTG
                  ************************************************************

HsCD00512985      CCTGCCTGGCACAAGTCCATCGTATCTTTCCGGCAGCTTCGGGAGGAGGTGCTAGGAGAA
NM_021830.4       CCTGCCTGGCACAAGTCCATCGTATCTTTCCGGCAGCTTCGGGAGGAGGTGCTAGGAGAA
                  ************************************************************

HsCD00512985      CTGTCAAATGTGGAGCAAGCAGCTGGCCTCCGCTGGAGCCGCTTTCCAGACCTCAATCGT
NM_021830.4       CTGTCAAATGTGGAGCAAGCAGCTGGCCTCCGCTGGAGCCGCTTTCCAGACCTCAATCGT
                  ************************************************************

HsCD00512985      ATCTTGAAGGGACATCGAAAGGGCGAGCTGACGGTCTTCACAGGGCCAACAGGCAGTGGA
NM_021830.4       ATCTTGAAGGGACATCGAAAGGGCGAGCTGACGGTCTTCACAGGGCCAACAGGCAGTGGA
                  ************************************************************

HsCD00512985      AAGACGACATTCATCAGTGAGTATGCCCTGGATTTGTGTTCCCAGGGGGTGAACACACTG
NM_021830.4       AAGACGACATTCATCAGTGAGTATGCCCTGGATTTGTGTTCCCAGGGGGTGAACACACTG
                  ************************************************************

HsCD00512985      TGGGGTAGCTTCGAGATCAGCAATGTGAGACTAGCCCGGGTCATGCTGACACAGTTTGCC
NM_021830.4       TGGGGTAGCTTCGAGATCAGCAATGTGAGACTAGCCCGGGTCATGCTGACACAGTTTGCC
                  ************************************************************

HsCD00512985      GAGGGGCGGCTGGAAGATCAACTGGACAAATATGATCACTGGGCTGACCGCTTTGAGGAC
NM_021830.4       GAGGGGCGGCTGGAAGATCAACTGGACAAATATGATCACTGGGCTGACCGCTTTGAGGAC
                  ************************************************************

HsCD00512985      CTGCCCCTCTATTTCATGACTTTCCATGGACAGCAAAGCATCAGGACTGTAATAGATACA
NM_021830.4       CTGCCCCTCTATTTCATGACTTTCCATGGACAGCAAAGCATCAGGACTGTAATAGATACA
                  ************************************************************

HsCD00512985      ATGCAACATGCAGTCTACGTCTATGACATTTGTCATGTGATCATCGACAACCTGCAGTTC
NM_021830.4       ATGCAACATGCAGTCTACGTCTATGACATTTGTCATGTGATCATCGACAACCTGCAGTTC
                  ************************************************************

HsCD00512985      ATGATGGGTCACGAGCAGCTGTCCACAGACAGGATCGCAGCTCAAGACTACATCATCGGG
NM_021830.4       ATGATGGGTCACGAGCAGCTGTCCACAGACAGGATCGCAGCTCAAGACTACATCATCGGG
                  ************************************************************

HsCD00512985      GTCTTTCGGAAGTTTGCAACAGACAATAACTGCCATGTGACACTGGTCATTCACCCCCGG
NM_021830.4       GTCTTTCGGAAGTTTGCAACAGACAATAACTGCCATGTGACACTGGTCATTCACCCCCGG
                  ************************************************************

HsCD00512985      AAAGAGGATGATGACAAGGAACTGCAGACAGCGTCCATTTTTGGCTCAGCCAAAGCAAGC
NM_021830.4       AAAGAGGATGATGACAAGGAACTGCAGACAGCGTCCATTTTTGGCTCAGCCAAAGCAAGC
                  ************************************************************

HsCD00512985      CAGGAAGCAGACAATGTTCTGATCCTGCAGGACAGGAAGCTGGTAACCGGGCCAGGGAAA
NM_021830.4       CAGGAAGCAGACAATGTTCTGATCCTGCAGGACAGGAAGCTGGTAACCGGGCCAGGGAAA
                  ************************************************************

HsCD00512985      CGGTATCTGCAGGTGTCCAAGAACCGCTTTGATGGAGATGTAGGTGTCTTCCCGCTTGAG
NM_021830.4       CGGTATCTGCAGGTGTCCAAGAACCGCTTTGATGGAGATGTAGGTGTCTTCCCGCTTGAG
                  ************************************************************

HsCD00512985      TTCAACAAGAACTCCCTCACCTTCTCCATTCCACCAAAGAACAAGGCCCGGCTCAAGAAG
NM_021830.4       TTCAACAAGAACTCCCTCACCTTCTCCATTCCACCAAAGAACAAGGCCCGGCTCAAGAAG
                  ************************************************************

HsCD00512985      ATCAAGGATGACACTGGACCAGTGGCCAAAAAGCCCTCTTCTGGCAAAAAGGGGGCTACG
NM_021830.4       ATCAAGGATGACACTGGACCAGTGGCCAAAAAGCCCTCTTCTGGCAAAAAGGGGGCTACG
                  ************************************************************

HsCD00512985      ACACAGAACTCTGAGATTTGCTCAGGCCAGGCCCCCACTCCCGACCAGCCAGACACCTCC
NM_021830.4       ACACAGAACTCTGAGATTTGCTCAGGCCAGGCCCCCACTCCCGACCAGCCAGACACCTCC
                  ************************************************************

HsCD00512985      AAGCGTTCAAAG---
NM_021830.4       AAGCGTTCAAAGTGA
                  ************