DNASU Plasmid | ZNF320 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF320

Gene Name: zinc finger protein 320

Original Clone ID:

ccsbBroadEn_05120

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC131555.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513051

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1528nts

Open reading frame: 1 to 1528

Reference Sequence Alignment

ATGGCTCTTTCTCAGGGTCTACTGACATTCAGGGATGTGGCCATAGAATTCTCTCAGGAG
GAGTGGAAATGCCTGGACCCTGCTCAGAGGACTCTATACAGAGACGTGATGCTGGAGAAT
TATAGGAACCTGGTCTCCCTGGATATCTCTTCCAAATGCATGATGAATACATTGTCATCA
ACAGGGCAAGGCAATACAGAAGTGATCCACACAGGGACATTGCAGAGACAAGCAAGTTAT
CACATTGGAGCATTTTGCTCCCAGGAAATTGAGAAAGACATTCATGACTTTGTGTTTCAG
TGGCAAGAAGATGAAACAAATGACCATGAAGCACCCATGACAGAAATAAAAAAGTTGACT
AGTAGTACAGACCGATATGATCAAAGGCATGCTGGAAACAAGCCTATTAAAGGTCAGCTT
GAATCAAGATTTCATTTGCATTTGCGAAGACATAGGAGAATTCATACTGGAGAGAAACCT
TACAAATGTGAAGAATGTGAGAAAGTTTTCAGTTGCAAATCACATCTTGAAATACATAGG
ATAATTCATACTGGAGAGAAACCATACAAATGTAAGGTTTGCGACAAGGCTTTTAAGCAT
GATTCACACCTGGCAAAACATACTAGAATTCACAGGGGAGACAAACATTACACATGTAAT
GAATGTGGCAAGGTTTTTGATCAAAAAGCAACCCTTGCATGTCATCATAGAAGTCATACT
GGAGAGAAACCTTATAAGTGTAATGAGTGTGGCAAGACCTTTAGTCAGACATCACACCTT
GTGTACCATCATAGACTGCATACTGGAGAGAAACCTTACAAATGTAATGAGTGTGGCAAG
ACCTTCGCTCGAAATTCAGTCCTCGTAATTCATAAGGCAGTTCATACTGCAGAGAAACCC
TATAAGTGTAATGAATGTGGCAAGGTTTTTAAGCAACGAGCAACTCTTGCAGGACATCGT
AGAGTTCACACTGGAGAGAAACCTTACAGATGTGAAGAATGCGACAAAGTTTTCAGTCGC
AAATCACATCTTGAAAGACATAGGAGGATTCATACTGGAGAGAAACCATACAAATGTAAG
GTTTGTGACAAGGCTTTCCGGAGTGATTCACGTCTTGCAGAACATCAGAGAGTTCATACT
GGAGAGAGACCTTACACATGTAATGAATGTGGCAAGGTTTTTAGTACAAAAGCGTACCTC
GCATGTCATCAAAAACTTCATACTGGAGAGAAACTTTACGAATGTGAAGAATGTGACAAA
GTTTACATTCGCAAATCACACCTTGAAAGACATAGGAGGATTCATACAGGAGAGAAACCT
CACAAGTGTGGTGATTGTGGTAAAGCCTTTAATTCACCTTCACACCTTATTAGGCATCAG
AGAATCCATACTGGACAGAAATCTTACAAATGTCATCAGTGTGGCAAGGTCTTTAGTCTG
AGGTCACTCCTTGCAGAACATCAGAAAATTCCTTTTGGAGACAATTGTTTCAAGTGCAAT
GAGTATAGCAAACCATCAAGCATTAAT

Protein sequence predicted based on nucleotide sequence

MALSQGLLTFRDVAIEFSQEEWKCLDPAQRTLYRDVMLENYRNLVSLDISSKCMMNTLSS
TGQGNTEVIHTGTLQRQASYHIGAFCSQEIEKDIHDFVFQWQEDETNDHEAPMTEIKKLT
SSTDRYDQRHAGNKPIKGQLESRFHLHLRRHRRIHTGEKPYKCEECEKVFSCKSHLEIHR
IIHTGEKPYKCKVCDKAFKHDSHLAKHTRIHRGDKHYTCNECGKVFDQKATLACHHRSHT
GEKPYKCNECGKTFSQTSHLVYHHRLHTGEKPYKCNECGKTFARNSVLVIHKAVHTAEKP
YKCNECGKVFKQRATLAGHRRVHTGEKPYRCEECDKVFSRKSHLERHRRIHTGEKPYKCK
VCDKAFRSDSRLAEHQRVHTGERPYTCNECGKVFSTKAYLACHQKLHTGEKLYECEECDK
VYIRKSHLERHRRIHTGEKPHKCGDCGKAFNSPSHLIRHQRIHTGQKSYKCHQCGKVFSL
RSLLAEHQKIPFGDNCFKCNEYSKPSSIN

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF320

Symbol Nomenclature :

ZNF320

SYNONYM :

ZFPL

Designation :

zinc finger gene 320|zinc finger protein like

Full Nomenclature :

zinc finger protein 320

GENEID :

162967

HGNC :

13842

MIM :

606427

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00513051      ATGGCTCTTTCTCAGGGTCTACTGACATTCAGGGATGTGGCCATAGAATTCTCTCAGGAG
NM_207333.2       ATGGCTCTTTCTCAGGGTCTACTGACATTCAGGGATGTGGCCATAGAATTCTCTCAGGAG
                  ************************************************************

HsCD00513051      GAGTGGAAATGCCTGGACCCTGCTCAGAGGACTCTATACAGAGACGTGATGCTGGAGAAT
NM_207333.2       GAGTGGAAATGCCTGGACCCTGCTCAGAGGACTCTATACAGAGACGTGATGCTGGAGAAT
                  ************************************************************

HsCD00513051      TATAGGAACCTGGTCTCCCTGGATATCTCTTCCAAATGCATGATGAATACATTGTCATCA
NM_207333.2       TATAGGAACCTGGTCTCCCTGGATATCTCTTCCAAATGCATGATGAATACATTGTCATCA
                  ************************************************************

HsCD00513051      ACAGGGCAAGGCAATACAGAAGTGATCCACACAGGGACATTGCAGAGACAAGCAAGTTAT
NM_207333.2       ACAGGGCAAGGCAATACAGAAGTGATCCACACAGGGACATTGCAGAGACAAGCAAGTTAT
                  ************************************************************

HsCD00513051      CACATTGGAGCATTTTGCTCCCAGGAAATTGAGAAAGACATTCATGACTTTGTGTTTCAG
NM_207333.2       CACATTGGAGCATTTTGCTCCCAGGAAATTGAGAAAGACATTCATGACTTTGTGTTTCAG
                  ************************************************************

HsCD00513051      TGGCAAGAAGATGAAACAAATGACCATGAAGCACCCATGACAGAAATAAAAAAGTTGACT
NM_207333.2       TGGCAAGAAGATGAAACAAATGACCATGAAGCACCCATGACAGAAATAAAAAAGTTGACT
                  ************************************************************

HsCD00513051      AGTAGTACAGACCGATATGATCAAAGGCATGCTGGAAACAAGCCTATTAAAGGTCAGCTT
NM_207333.2       AGTAGTACAGACCGATATGATCAAAGGCATGCTGGAAACAAGCCTATTAAAGGTCAGCTT
                  ************************************************************

HsCD00513051      GAATCAAGATTTCATTTGCATTTGCGAAGACATAGGAGAATTCATACTGGAGAGAAACCT
NM_207333.2       GAATCAAGATTTCATTTGCATTTGCGAAGACATAGGAGAATTCATACTGGAGAGAAACCT
                  ************************************************************

HsCD00513051      TACAAATGTGAAGAATGTGAGAAAGTTTTCAGTTGCAAATCACATCTTGAAATACATAGG
NM_207333.2       TACAAATGTGAAGAATGTGAGAAAGTTTTCAGTTGCAAATCACATCTTGAAATACATAGG
                  ************************************************************

HsCD00513051      ATAATTCATACTGGAGAGAAACCATACAAATGTAAGGTTTGCGACAAGGCTTTTAAGCAT
NM_207333.2       ATAATTCATACTGGAGAGAAACCATACAAATGTAAGGTTTGCGACAAGGCTTTTAAGCAT
                  ************************************************************

HsCD00513051      GATTCACACCTGGCAAAACATACTAGAATTCACAGGGGAGACAAACATTACACATGTAAT
NM_207333.2       GATTCACACCTGGCAAAACATACTAGAATTCACAGGGGAGACAAACATTACACATGTAAT
                  ************************************************************

HsCD00513051      GAATGTGGCAAGGTTTTTGATCAAAAAGCAACCCTTGCATGTCATCATAGAAGTCATACT
NM_207333.2       GAATGTGGCAAGGTTTTTGATCAAAAAGCAACCCTTGCATGTCATCATAGAAGTCATACT
                  ************************************************************

HsCD00513051      GGAGAGAAACCTTATAAGTGTAATGAGTGTGGCAAGACCTTTAGTCAGACATCACACCTT
NM_207333.2       GGAGAGAAACCTTATAAGTGTAATGAGTGTGGCAAGACCTTTAGTCAGACATCACACCTT
                  ************************************************************

HsCD00513051      GTGTACCATCATAGACTGCATACTGGAGAGAAACCTTACAAATGTAATGAGTGTGGCAAG
NM_207333.2       GTGTACCATCATAGACTGCATACTGGAGAGAAACCTTACAAATGTAATGAGTGTGGCAAG
                  ************************************************************

HsCD00513051      ACCTTCGCTCGAAATTCAGTCCTCGTAATTCATAAGGCAGTTCATACTGCAGAGAAACCC
NM_207333.2       ACCTTCGCTCGAAATTCAGTCCTCGTAATTCATAAGGCAGTTCATACTGCAGAGAAACCC
                  ************************************************************

HsCD00513051      TATAAGTGTAATGAATGTGGCAAGGTTTTTAAGCAACGAGCAACTCTTGCAGGACATCGT
NM_207333.2       TATAAGTGTAATGAATGTGGCAAGGTTTTTAAGCAACGAGCAACTCTTGCAGGACATCGT
                  ************************************************************

HsCD00513051      AGAGTTCACACTGGAGAGAAACCTTACAGATGTGAAGAATGCGACAAAGTTTTCAGTCGC
NM_207333.2       AGAGTTCACACTGGAGAGAAACCTTACAGATGTGAAGAATGCGACAAAGTTTTCAGTCGC
                  ************************************************************

HsCD00513051      AAATCACATCTTGAAAGACATAGGAGGATTCATACTGGAGAGAAACCATACAAATGTAAG
NM_207333.2       AAATCACATCTTGAAAGACATAGGAGGATTCATACTGGAGAGAAACCATACAAATGTAAG
                  ************************************************************

HsCD00513051      GTTTGTGACAAGGCTTTCCGGAGTGATTCACGTCTTGCAGAACATCAGAGAGTTCATACT
NM_207333.2       GTTTGTGACAAGGCTTTCCGGAGTGATTCACGTCTTGCAGAACATCAGAGAGTTCATACT
                  ************************************************************

HsCD00513051      GGAGAGAGACCTTACACATGTAATGAATGTGGCAAGGTTTTTAGTACAAAAGCGTACCTC
NM_207333.2       GGAGAGAGACCTTACACATGTAATGAATGTGGCAAGGTTTTTAGTACAAAAGCGTACCTC
                  ************************************************************

HsCD00513051      GCATGTCATCAAAAACTTCATACTGGAGAGAAACTTTACGAATGTGAAGAATGTGACAAA
NM_207333.2       GCATGTCATCAAAAACTTCATACTGGAGAGAAACTTTACGAATGTGAAGAATGTGACAAA
                  ************************************************************

HsCD00513051      GTTTACATTCGCAAATCACACCTTGAAAGACATAGGAGGATTCATACAGGAGAGAAACCT
NM_207333.2       GTTTACATTCGCAAATCACACCTTGAAAGACATAGGAGGATTCATACAGGAGAGAAACCT
                  ************************************************************

HsCD00513051      CACAAGTGTGGTGATTGTGGTAAAGCCTTTAATTCACCTTCACACCTTATTAGGCATCAG
NM_207333.2       CACAAGTGTGGTGATTGTGGTAAAGCCTTTAATTCACCTTCACACCTTATTAGGCATCAG
                  ************************************************************

HsCD00513051      AGAATCCATACTGGACAGAAATCTTACAAATGTCATCAGTGTGGCAAGGTCTTTAGTCTG
NM_207333.2       AGAATCCATACTGGACAGAAATCTTACAAATGTCATCAGTGTGGCAAGGTCTTTAGTCTG
                  ************************************************************

HsCD00513051      AGGTCACTCCTTGCAGAACATCAGAAAATTCCTTTTGGAGACAATTGTTTCAAGTGCAAT
NM_207333.2       AGGTCACTCCTTGCAGAACATCAGAAAATTCCTTTTGGAGACAATTGTTTCAAGTGCAAT
                  ************************************************************

HsCD00513051      GAGTATAGCAAACCATCAAGCATTAAT---
NM_207333.2       GAGTATAGCAAACCATCAAGCATTAATTGA
                  ***************************