DNASU Plasmid | ZNF490 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF490

Gene Name: zinc finger protein 490

Original Clone ID:

ccsbBroadEn_08734

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC038586.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513122

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1588nts

Open reading frame: 1 to 1588

Reference Sequence Alignment

ATGCGCAGGAATTCCAGTCTCAGTTTCCAGATGGAGCGACCCCTCGAGGAGCAAGTCCAG
AGCAAGTGGTCGTCTAGTCAAGGCCGCACAGGAACAGGAGGGTCTGATGTCCTCCAGATG
CAGAACAGTGAACACCATGGACAAAGCATCAAGACTCAAACTGACTCCATCTCCCTTGAG
GATGTGGCTGTGAACTTCACCCTGGAGGAGTGGGCTTTGCTGGATCCTGGCCAGAGGAAT
ATCTACAGAGATGTGATGCGGGCAACCTTCAAGAACCTGGCCTGTATAGGGGAAAAATGG
AAAGACCAGGATATTGAAGATGAACACAAAAACCAGGGAAGAAATCTAAGAAGTCCTATG
GTTGAAGCACTCTGTGAAAATAAAGAAGATTGTCCATGTGGAAAAAGCACTAGCCAGATT
CCTGATCTTAATACGAACCTGGAAACTCCTACTGGATTAAAACCATGTGACTGCAGTGTG
TGTGGGGAAGTCTTCATGCATCAGGTCTCCCTTAATAGGCACATGAGATCTCACACTGAA
CAGAAACCAAATGAGTGTCACGAATATGGAGAGAAGCCACATAAATGCAAAGAATGTGGG
AAAACCTTCACTCGCAGCTCCAGTATTCGAACCCATGAAAGAATTCACACTGGAGAGAAA
CCCTATGAATGTAAGGAATGTGGCAAAGCCTTCGCATTTCTCTTTTCCTTTCGAAACCAT
ATAAGAATTCATACTGGAGAGACACCCTATGAATGTAAGGAATGTGGGAAGGCATTCAGA
TATCTCACTGCTCTTCGGCGCCATGAAAAAAATCACACTGGAGAGAAACCCTACAAATGT
AAACAGTGTGGAAAAGCCTTTATATATTACCAGCCTTTTCTAACCCATGAAAGGACTCAC
ACTGGAGAGAAACCTTATGAATGTAAGCAATGTGGGAAAGCCTTCAGTTGTCCCACGTAC
TTACGGAGTCATGAGAAAACTCATACTGGAGAGAAACCTTTTGTATGTAGGGAATGTGGG
AGAGCCTTCTTTTCTCACTCAAGCCTTCGAAAACACGTGAAAACCCACACCGGAGTTCAA
CCTTATACATGTAAGAAATGTGGGGAAGCCTTCAAGTCATCTAGTTCCTGTGAAGTGCAC
GAAAGAACTCATTTTGGAGAAAAACCCTATGAATGTAAACAATGTGGTAAAGCCTTCAAT
TCTTCAAGTTACCTTCAGTTGCACGAAAGAGTTCACACTGGCGAGAAAACTTACGAATGT
AAAGAATGTGGTAAAGCCTTTCTTTATTCCACTCACTTTCGAATCCATGAAAGAACCCAT
ACTAGAGAGAAACCCTATGAATGCAAACAGTGTGGTCGGGTCTTCATTTACTTCAGTCAC
CTTCGAAGGCACGAAAGAAGTCACACTGGAGTGAAACCATGTGAGTGTAAGCAGTGTGGC
AAAGCTTTCACTTGTTTAAATTCCCTGAAAGTACACAAAAGAATTCATACTGGAGAAAGA
CCCTTTCAGTGTAGACAATGTGGTAAAGCCTTCAGTTACTCAAAGTCTTTGCACGTGCAC
GAAAGGACTCATAGTAGACAGAAGCCC

Protein sequence predicted based on nucleotide sequence

MRRNSSLSFQMERPLEEQVQSKWSSSQGRTGTGGSDVLQMQNSEHHGQSIKTQTDSISLE
DVAVNFTLEEWALLDPGQRNIYRDVMRATFKNLACIGEKWKDQDIEDEHKNQGRNLRSPM
VEALCENKEDCPCGKSTSQIPDLNTNLETPTGLKPCDCSVCGEVFMHQVSLNRHMRSHTE
QKPNECHEYGEKPHKCKECGKTFTRSSSIRTHERIHTGEKPYECKECGKAFAFLFSFRNH
IRIHTGETPYECKECGKAFRYLTALRRHEKNHTGEKPYKCKQCGKAFIYYQPFLTHERTH
TGEKPYECKQCGKAFSCPTYLRSHEKTHTGEKPFVCRECGRAFFSHSSLRKHVKTHTGVQ
PYTCKKCGEAFKSSSSCEVHERTHFGEKPYECKQCGKAFNSSSYLQLHERVHTGEKTYEC
KECGKAFLYSTHFRIHERTHTREKPYECKQCGRVFIYFSHLRRHERSHTGVKPCECKQCG
KAFTCLNSLKVHKRIHTGERPFQCRQCGKAFSYSKSLHVHERTHSRQKP

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF490

Symbol Nomenclature :

ZNF490

Full Nomenclature :

zinc finger protein 490

GENEID :

57474

GenBank Accession :

EU446833

HGNC :

23705

Target GenBank:

EU446833

Reference Sequence Alignment

  

HsCD00513122      ATGCGCAGGAATTCCAGTCTCAGTTTCCAGATGGAGCGACCCCTCGAGGAGCAAGTCCAG
NM_020714.2       ATGCGCAGGAATTCCAGTCTCAGTTTCCAGATGGAGCGACCCCTCGAGGAGCAAGTCCAG
                  ************************************************************

HsCD00513122      AGCAAGTGGTCGTCTAGTCAAGGCCGCACAGGAACAGGAGGGTCTGATGTCCTCCAGATG
NM_020714.2       AGCAAGTGGTCGTCTAGTCAAGGCCGCACAGGAACAGGAGGGTCTGATGTCCTCCAGATG
                  ************************************************************

HsCD00513122      CAGAACAGTGAACACCATGGACAAAGCATCAAGACTCAAACTGACTCCATCTCCCTTGAG
NM_020714.2       CAGAACAGTGAACACCATGGACAAAGCATCAAGACTCAAACTGACTCCATCTCCCTTGAG
                  ************************************************************

HsCD00513122      GATGTGGCTGTGAACTTCACCCTGGAGGAGTGGGCTTTGCTGGATCCTGGCCAGAGGAAT
NM_020714.2       GATGTGGCTGTGAACTTCACCCTGGAGGAGTGGGCTTTGCTGGATCCTGGCCAGAGGAAT
                  ************************************************************

HsCD00513122      ATCTACAGAGATGTGATGCGGGCAACCTTCAAGAACCTGGCCTGTATAGGGGAAAAATGG
NM_020714.2       ATCTACAGAGATGTGATGCGGGCAACCTTCAAGAACCTGGCCTGTATAGGGGAAAAATGG
                  ************************************************************

HsCD00513122      AAAGACCAGGATATTGAAGATGAACACAAAAACCAGGGAAGAAATCTAAGAAGTCCTATG
NM_020714.2       AAAGACCAGGATATTGAAGATGAACACAAAAACCAGGGAAGAAATCTAAGAAGTCCTATG
                  ************************************************************

HsCD00513122      GTTGAAGCACTCTGTGAAAATAAAGAAGATTGTCCATGTGGAAAAAGCACTAGCCAGATT
NM_020714.2       GTTGAAGCACTCTGTGAAAATAAAGAAGATTGTCCATGTGGAAAAAGCACTAGCCAGATT
                  ************************************************************

HsCD00513122      CCTGATCTTAATACGAACCTGGAAACTCCTACTGGATTAAAACCATGTGACTGCAGTGTG
NM_020714.2       CCTGATCTTAATACGAACCTGGAAACTCCTACTGGATTAAAACCATGTGACTGCAGTGTG
                  ************************************************************

HsCD00513122      TGTGGGGAAGTCTTCATGCATCAGGTCTCCCTTAATAGGCACATGAGATCTCACACTGAA
NM_020714.2       TGTGGGGAAGTCTTCATGCATCAGGTCTCCCTTAATAGGCACATGAGATCTCACACTGAA
                  ************************************************************

HsCD00513122      CAGAAACCAAATGAGTGTCACGAATATGGAGAGAAGCCACATAAATGCAAAGAATGTGGG
NM_020714.2       CAGAAACCAAATGAGTGTCACGAATATGGAGAGAAGCCACATAAATGCAAAGAATGTGGG
                  ************************************************************

HsCD00513122      AAAACCTTCACTCGCAGCTCCAGTATTCGAACCCATGAAAGAATTCACACTGGAGAGAAA
NM_020714.2       AAAACCTTCACTCGCAGCTCCAGTATTCGAACCCATGAAAGAATTCACACTGGAGAGAAA
                  ************************************************************

HsCD00513122      CCCTATGAATGTAAGGAATGTGGCAAAGCCTTCGCATTTCTCTTTTCCTTTCGAAACCAT
NM_020714.2       CCCTATGAATGTAAGGAATGTGGCAAAGCCTTCGCATTTCTCTTTTCCTTTCGAAACCAT
                  ************************************************************

HsCD00513122      ATAAGAATTCATACTGGAGAGACACCCTATGAATGTAAGGAATGTGGGAAGGCATTCAGA
NM_020714.2       ATAAGAATTCATACTGGAGAGACACCCTATGAATGTAAGGAATGTGGGAAGGCATTCAGA
                  ************************************************************

HsCD00513122      TATCTCACTGCTCTTCGGCGCCATGAAAAAAATCACACTGGAGAGAAACCCTACAAATGT
NM_020714.2       TATCTCACTGCTCTTCGGCGCCATGAAAAAAATCACACTGGAGAGAAACCCTACAAATGT
                  ************************************************************

HsCD00513122      AAACAGTGTGGAAAAGCCTTTATATATTACCAGCCTTTTCTAACCCATGAAAGGACTCAC
NM_020714.2       AAACAGTGTGGAAAAGCCTTTATATATTACCAGCCTTTTCTAACCCACGAAAGGACTCAC
                  *********************************************** ************

HsCD00513122      ACTGGAGAGAAACCTTATGAATGTAAGCAATGTGGGAAAGCCTTCAGTTGTCCCACGTAC
NM_020714.2       ACTGGAGAGAAACCTTATGAATGTAAGCAATGTGGGAAAGCCTTCAGTTGTCCCACGTAC
                  ************************************************************

HsCD00513122      TTACGGAGTCATGAGAAAACTCATACTGGAGAGAAACCTTTTGTATGTAGGGAATGTGGG
NM_020714.2       TTACGGAGTCATGAGAAAACTCATACTGGAGAGAAACCTTTTGTATGTAGGGAATGTGGG
                  ************************************************************

HsCD00513122      AGAGCCTTCTTTTCTCACTCAAGCCTTCGAAAACACGTGAAAACCCACACCGGAGTTCAA
NM_020714.2       AGAGCCTTCTTTTCTCACTCAAGCCTTCGAAAACACGTGAAAACCCACACCGGAGTTCAA
                  ************************************************************

HsCD00513122      CCTTATACATGTAAGAAATGTGGGGAAGCCTTCAAGTCATCTAGTTCCTGTGAAGTGCAC
NM_020714.2       CCTTATACATGTAAGAAATGTGGGGAAGCCTTCAAGTCATCTAGTTCCTGTGAAGTGCAC
                  ************************************************************

HsCD00513122      GAAAGAACTCATTTTGGAGAAAAACCCTATGAATGTAAACAATGTGGTAAAGCCTTCAAT
NM_020714.2       GAAAGAACTCATTTTGGAGAAAAACCCTATGAATGTAAACAATGTGGTAAAGCCTTCAAT
                  ************************************************************

HsCD00513122      TCTTCAAGTTACCTTCAGTTGCACGAAAGAGTTCACACTGGCGAGAAAACTTACGAATGT
NM_020714.2       TCTTCAAGTTACCTTCAGTTGCACGAAAGAGTTCACACTGGCGAGAAAACTTACGAATGT
                  ************************************************************

HsCD00513122      AAAGAATGTGGTAAAGCCTTTCTTTATTCCACTCACTTTCGAATCCATGAAAGAACCCAT
NM_020714.2       AAAGAATGTGGTAAAGCCTTTCTTTATTCCACTCACTTTCGAATCCATGAAAGAACCCAT
                  ************************************************************

HsCD00513122      ACTAGAGAGAAACCCTATGAATGCAAACAGTGTGGTCGGGTCTTCATTTACTTCAGTCAC
NM_020714.2       ACTAGAGAGAAACCCTATGAATGCAAACAGTGTGGTCGGGTCTTCATTTACTTCAGTCAC
                  ************************************************************

HsCD00513122      CTTCGAAGGCACGAAAGAAGTCACACTGGAGTGAAACCATGTGAGTGTAAGCAGTGTGGC
NM_020714.2       CTTCGAAGGCACGAAAGAAGTCACACTGGAGTGAAACCATGTGAGTGTAAGCAGTGTGGC
                  ************************************************************

HsCD00513122      AAAGCTTTCACTTGTTTAAATTCCCTGAAAGTACACAAAAGAATTCATACTGGAGAAAGA
NM_020714.2       AAAGCTTTCACTTGTTTAAATTCCCTGAAAGTACACAAAAGAATTCATACTGGAGAAAGA
                  ************************************************************

HsCD00513122      CCCTTTCAGTGTAGACAATGTGGTAAAGCCTTCAGTTACTCAAAGTCTTTGCACGTGCAC
NM_020714.2       CCCTTTCAGTGTAGACAATGTGGTAAAGCCTTCAGTTACTCAAAGTCTTTGCACGTGCAC
                  ************************************************************

HsCD00513122      GAAAGGACTCATAGTAGACAGAAGCCC---
NM_020714.2       GAAAGGACTCATAGTAGACAGAAGCCCTAA
                  ***************************