DNASU Plasmid | WHSC2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: NELFA

Gene Name: negative elongation factor complex member A

Original Clone ID:

ccsbBroadEn_11220

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC002764.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513128

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1585nts

Open reading frame: 1 to 1585

Reference Sequence Alignment

ATGGCGTCCATGCGGGAGAGCGACACGGGCCTGTGGCTGCACAACAAGCTGGGGGCCACG
GACGAGCTGTGGGCGCCGCCCAGCATCGCGTCCCTGCTCACGGCCGCGGTCATCGACAAC
ATCCGTCTCTGCTTCCATGGCCTCTCGTCGGCAGTGAAGCTCAAGTTGCTACTCGGGACG
CTGCACCTCCCGCGCCGCACGGTGGACGAGATGAAGGGCGCCCTAATGGAGATCATCCAG
CTCGCCAGCCTCGACTCGGACCCCTGGGTGCTCATGGTCGCCGACATCTTGAAGTCCTTT
CCGGACACAGGCTCGCTTAACCTGGAGCTGGAGGAGCAGAATCCCAACGTTCAGGATATT
TTGGGAGAACTTAGAGAAAAGGTGGGTGAGTGTGAAGCGTCTGCCATGCTGCCACTGGAG
TGCCAGTACTTGAACAAAAACGCCCTGACGACCCTCGCGGGACCCCTCACTCCCCCGGTG
AAGCATTTTCAGTTAAAGCGGAAACCCAAGAGCGCCACGCTGCGGGCGGAGCTGCTGCAG
AAGTCCACGGAGACCGCCCAGCAGTTGAAGCGGAGCGCCGGGGTGCCCTTCCACGCCAAG
GGCCGGGGGCTGCTGCGGAAGATGGACACCACCACCCCACTCAAAGGCATCCCGAAGCAG
GCGCCCTTCAGAAGCCCCACGGCGCCCAGCGTCTTCAGCCCCACAGGGAACCGGACCCCC
ATCCCGCCTTCCAGGACGCTGCTGCGGAAGGAACGAGGTGTGAAGCTGCTGGACATCTCT
GAGCTGGATATGGTTGGCGCTGGCCGAGAGGCGAAGCGGAGAAGGAAGACTCTCGATGCG
GAGGTGGTGGAGAAGCCGGCCAAGGAGGAAACGGTGGTGGAGAACGCCACCCCGGACTAC
GCAGCCGGCCTGGTGTCCACGCAGAAACTTGGGTCCCTGAACAATGAGCCTGCGCTGCCC
TCCACGAGCTACCTTCCCTCCACGCCCAGCGTGGTTCCCGCCTCCTCCTACATCCCCAGC
TCCGAGACACCCCCAGCCCCATCTTCCCGGGAAGCCAGCCGCCCACCAGAGGAGCCCAGC
GCCCCGAGCCCCACGTTGCCAGCGCAGTTCAAGCAGCGGGCGCCCATGTACAACAGCGGC
CTGAGCCCTGCCACACCCACGCCTGCGGCGCCCACCTCGCCTCTGACACCCACCACACCT
CCGGCTGTCGCCCCTACCACTCAGACACCCCCGGTTGCCATGGTGGCCCCGCAGACCCAG
GCCCCTGCTCAGCAGCAGCCTAAGAAGAACCTGTCCCTCACGAGAGAGCAGATGTTCGCT
GCCCAGGAGATGTTCAAGACGGCCAACAAAGTCACGCGGCCCGAGAAGGCCCTCATCCTG
GGCTTCATGGCCGGCTCCCGAGAGAACCCGTGCCAGGAGCAGGGGGACGTGATCCAGATC
AAGCTGAGCGAGCACACGGAGGACCTGCCCAAGGCGGACGGCCAGGGTAGCACAACCATG
CTGGTGGACACAGTGTTTGAGATGAACTATGCCACGGGCCAGTGGACGCGCTTCAAGAAG
TACAAGCCCATGACCAATGTGTCC

Protein sequence predicted based on nucleotide sequence

MASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGT
LHLPRRTVDEMKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQDI
LGELREKVGECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQ
KSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTAPSVFSPTGNRTP
IPPSRTLLRKERGVKLLDISELDMVGAGREAKRRRKTLDAEVVEKPAKEETVVENATPDY
AAGLVSTQKLGSLNNEPALPSTSYLPSTPSVVPASSYIPSSETPPAPSSREASRPPEEPS
APSPTLPAQFKQRAPMYNSGLSPATPTPAAPTSPLTPTTPPAVAPTTQTPPVAMVAPQTQ
APAQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCQEQGDVIQI
KLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

NELFA

Symbol Nomenclature :

NELFA

SYNONYM :

NELF-A; WHSC2

Designation :

negative elongation factor A|wolf-Hirschhorn syndrome candidate 2 protein

Full Nomenclature :

negative elongation factor complex member A

GENEID :

7469

Locus Tag :

P/OKcl.15

GenBank Accession :

EU831506

HGNC :

12768

MIM :

606026

Vega :

OTTHUMG00000089967

Target GenBank:

EU831506

Reference Sequence Alignment

  

HsCD00513128      ---------------------------------ATGGCGTCCATGCGGGAGAGCGACACG
NM_005663.4       ATGCCGGGGCAGCGGCGCGCGCTTTCCCCCAAGATGGCGTCCATGCGGGAGAGCGACACG
                                                   ***************************

HsCD00513128      GGCCTGTGGCTGCACAACAAGCTGGGGGCCACGGACGAGCTGTGGGCGCCGCCCAGCATC
NM_005663.4       GGCCTGTGGCTGCACAACAAGCTGGGGGCCACGGACGAGCTGTGGGCGCCGCCCAGCATC
                  ************************************************************

HsCD00513128      GCGTCCCTGCTCACGGCCGCGGTCATCGACAACATCCGTCTCTGCTTCCATGGCCTCTCG
NM_005663.4       GCGTCCCTGCTCACGGCCGCGGTCATCGACAACATCCGTCTCTGCTTCCATGGCCTCTCG
                  ************************************************************

HsCD00513128      TCGGCAGTGAAGCTCAAGTTGCTACTCGGGACGCTGCACCTCCCGCGCCGCACGGTGGAC
NM_005663.4       TCGGCAGTGAAGCTCAAGTTGCTACTCGGGACGCTGCACCTCCCGCGCCGCACGGTGGAC
                  ************************************************************

HsCD00513128      GAGATGAAGGGCGCCCTAATGGAGATCATCCAGCTCGCCAGCCTCGACTCGGACCCCTGG
NM_005663.4       GAGATGAAGGGCGCCCTAATGGAGATCATCCAGCTCGCCAGCCTCGACTCGGACCCCTGG
                  ************************************************************

HsCD00513128      GTGCTCATGGTCGCCGACATCTTGAAGTCCTTTCCGGACACAGGCTCGCTTAACCTGGAG
NM_005663.4       GTGCTCATGGTCGCCGACATCTTGAAGTCCTTTCCGGACACAGGCTCGCTTAACCTGGAG
                  ************************************************************

HsCD00513128      CTGGAGGAGCAGAATCCCAACGTTCAGGATATTTTGGGAGAACTTAGAGAAAAGGTGGGT
NM_005663.4       CTGGAGGAGCAGAATCCCAACGTTCAGGATATTTTGGGAGAACTTAGAGAAAAGGTGGGT
                  ************************************************************

HsCD00513128      GAGTGTGAAGCGTCTGCCATGCTGCCACTGGAGTGCCAGTACTTGAACAAAAACGCCCTG
NM_005663.4       GAGTGTGAAGCGTCTGCCATGCTGCCACTGGAGTGCCAGTACTTGAACAAAAACGCCCTG
                  ************************************************************

HsCD00513128      ACGACCCTCGCGGGACCCCTCACTCCCCCGGTGAAGCATTTTCAGTTAAAGCGGAAACCC
NM_005663.4       ACGACCCTCGCGGGACCCCTCACTCCCCCGGTGAAGCATTTTCAGTTAAAGCGGAAACCC
                  ************************************************************

HsCD00513128      AAGAGCGCCACGCTGCGGGCGGAGCTGCTGCAGAAGTCCACGGAGACCGCCCAGCAGTTG
NM_005663.4       AAGAGCGCCACGCTGCGGGCGGAGCTGCTGCAGAAGTCCACGGAGACCGCCCAGCAGTTG
                  ************************************************************

HsCD00513128      AAGCGGAGCGCCGGGGTGCCCTTCCACGCCAAGGGCCGGGGGCTGCTGCGGAAGATGGAC
NM_005663.4       AAGCGGAGCGCCGGGGTGCCCTTCCACGCCAAGGGCCGGGGGCTGCTGCGGAAGATGGAC
                  ************************************************************

HsCD00513128      ACCACCACCCCACTCAAAGGCATCCCGAAGCAGGCGCCCTTCAGAAGCCCCACGGCGCCC
NM_005663.4       ACCACCACCCCACTCAAAGGCATCCCGAAGCAGGCGCCCTTCAGAAGCCCCACGGCGCCC
                  ************************************************************

HsCD00513128      AGCGTCTTCAGCCCCACAGGGAACCGGACCCCCATCCCGCCTTCCAGGACGCTGCTGCGG
NM_005663.4       AGCGTCTTCAGCCCCACAGGGAACCGGACCCCCATCCCGCCTTCCAGGACGCTGCTGCGG
                  ************************************************************

HsCD00513128      AAGGAACGAGGTGTGAAGCTGCTGGACATCTCTGAGCTGGATATGGTTGGCGCTGGCCGA
NM_005663.4       AAGGAACGAGGTGTGAAGCTGCTGGACATCTCTGAGCTGGATATGGTTGGCGCTGGCCGA
                  ************************************************************

HsCD00513128      GAGGCGAAGCGGAGAAGGAAGACTCTCGATGCGGAGGTGGTGGAGAAGCCGGCCAAGGAG
NM_005663.4       GAGGCGAAGCGGAGAAGGAAGACTCTCGATGCGGAGGTGGTGGAGAAGCCGGCCAAGGAG
                  ************************************************************

HsCD00513128      GAAACGGTGGTGGAGAACGCCACCCCGGACTACGCAGCCGGCCTGGTGTCCACGCAGAAA
NM_005663.4       GAAACGGTGGTGGAGAACGCCACCCCGGACTACGCAGCCGGCCTGGTGTCCACGCAGAAA
                  ************************************************************

HsCD00513128      CTTGGGTCCCTGAACAATGAGCCTGCGCTGCCCTCCACGAGCTACCTTCCCTCCACGCCC
NM_005663.4       CTTGGGTCCCTGAACAATGAGCCTGCGCTGCCCTCCACGAGCTACCTTCCCTCCACGCCC
                  ************************************************************

HsCD00513128      AGCGTGGTTCCCGCCTCCTCCTACATCCCCAGCTCCGAGACACCCCCAGCCCCATCTTCC
NM_005663.4       AGCGTGGTTCCCGCCTCCTCCTACATCCCCAGCTCCGAGACGCCCCCAGCCCCATCTTCC
                  *****************************************.******************

HsCD00513128      CGGGAAGCCAGCCGCCCACCAGAGGAGCCCAGCGCCCCGAGCCCCACGTTGCCAGCGCAG
NM_005663.4       CGGGAAGCCAGCCGCCCACCAGAGGAGCCCAGCGCCCCGAGCCCCACGTTGCCAGCGCAG
                  ************************************************************

HsCD00513128      TTCAAGCAGCGGGCGCCCATGTACAACAGCGGCCTGAGCCCTGCCACACCCACGCCTGCG
NM_005663.4       TTCAAGCAGCGGGCGCCCATGTACAACAGCGGCCTGAGCCCTGCCACACCCACGCCTGCG
                  ************************************************************

HsCD00513128      GCGCCCACCTCGCCTCTGACACCCACCACACCTCCGGCTGTCGCCCCTACCACTCAGACA
NM_005663.4       GCGCCCACCTCGCCTCTGACACCCACCACACCTCCGGCTGTCGCCCCTACCACTCAGACA
                  ************************************************************

HsCD00513128      CCCCCGGTTGCCATGGTGGCCCCGCAGACCCAGGCCCCTGCTCAGCAGCAGCCTAAGAAG
NM_005663.4       CCCCCGGTTGCCATGGTGGCCCCGCAGACCCAGGCCCCTGCTCAGCAGCAGCCTAAGAAG
                  ************************************************************

HsCD00513128      AACCTGTCCCTCACGAGAGAGCAGATGTTCGCTGCCCAGGAGATGTTCAAGACGGCCAAC
NM_005663.4       AACCTGTCCCTCACGAGAGAGCAGATGTTCGCTGCCCAGGAGATGTTCAAGACGGCCAAC
                  ************************************************************

HsCD00513128      AAAGTCACGCGGCCCGAGAAGGCCCTCATCCTGGGCTTCATGGCCGGCTCCCGAGAGAAC
NM_005663.4       AAAGTCACGCGGCCCGAGAAGGCCCTCATCCTGGGCTTCATGGCCGGCTCCCGAGAGAAC
                  ************************************************************

HsCD00513128      CCGTGCCAGGAGCAGGGGGACGTGATCCAGATCAAGCTGAGCGAGCACACGGAGGACCTG
NM_005663.4       CCGTGCCAGGAGCAGGGGGACGTGATCCAGATCAAGCTGAGCGAGCACACGGAGGACCTG
                  ************************************************************

HsCD00513128      CCCAAGGCGGACGGCCAGGGTAGCACAACCATGCTGGTGGACACAGTGTTTGAGATGAAC
NM_005663.4       CCCAAGGCGGACGGCCAGGGTAGCACAACCATGCTGGTGGACACAGTGTTTGAGATGAAC
                  ************************************************************

HsCD00513128      TATGCCACGGGCCAGTGGACGCGCTTCAAGAAGTACAAGCCCATGACCAATGTGTCC---
NM_005663.4       TATGCCACGGGCCAGTGGACGCGCTTCAAGAAGTACAAGCCCATGACCAATGTGTCCTAG
                  *********************************************************

Pathways

Reactome :

Abortive elongation of HIV-1 transcript in the absence of Tat

Reactome :

Disease

Reactome :

Elongation arrest and recovery

Reactome :

Formation of HIV elongation complex in the absence of HIV Tat

Reactome :

Formation of HIV-1 elongation complex containing HIV-1 Tat

Reactome :

Formation of RNA Pol II elongation complex

Reactome :

Formation of the Early Elongation Complex

Reactome :

Formation of the HIV-1 Early Elongation Complex

Reactome :

Gene Expression

Reactome :

HIV elongation arrest and recovery

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

HIV Transcription Elongation

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Pausing and recovery of HIV elongation

Reactome :

Pausing and recovery of Tat-mediated HIV elongation

Reactome :

RNA Polymerase II Pre-transcription Events

Reactome :

RNA Polymerase II Transcription

Reactome :

RNA Polymerase II Transcription Elongation

Reactome :

Tat-mediated elongation of the HIV-1 transcript

Reactome :

Tat-mediated HIV elongation arrest and recovery

Reactome :

Transcription

Reactome :

Transcription of the HIV genome

Protein

HPRD :

12080

Clone

Cloning Information :

669863

Original Clone ID :

ccsbBroadEn_11220

Organism

Species Specific ID:

7469

Genome

Chromosome :

Map Location :

4p16.3

Ensembl :

ENSG00000185049

Reagents

Labome :

NELFA-antibody