DNASU Plasmid | SIGLEC5 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: SIGLEC5

Gene Name: sialic acid binding Ig-like lectin 5

Original Clone ID:

ccsbBroadEn_07297

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC029896.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513343

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1653nts

Open reading frame: 1 to 1653

Reference Sequence Alignment

ATGCTGCCCCTGCTGCTGCTGCCCCTGCTGTGGGGGGGGTCCCTGCAGGAGAAGCCAGTG
TACGAGCTGCAAGTGCAGAAGTCGGTGACGGTGCAGGAGGGCCTGTGCGTCCTTGTGCCC
TGCTCCTTCTCTTACCCCTGGAGATCCTGGTATTCCTCTCCCCCACTCTACGTCTACTGG
TTCCGGGACGGGGAGATCCCATACTACGCTGAGGTTGTGGCCACAAACAACCCAGACAGA
AGAGTGAAGCCAGAGACCCAGGGCCGATTCCGCCTCCTTGGGGATGTCCAGAAGAAGAAC
TGCTCCCTGAGCATCGGAGATGCCAGAATGGAGGACACGGGAAGCTATTTCTTCCGCGTG
GAGAGAGGAAGGGATGTAAAATATAGCTACCAACAGAATAAGCTGAACTTGGAGGTGACA
GCCCTGATAGAGAAACCCGACATCCACTTTCTGGAGCCTCTGGAGTCCGGCCGCCCCACA
AGGCTGAGCTGCAGCCTTCCAGGATCCTGTGAAGCGGGACCACCTCTCACATTCTCCTGG
ACGGGGAATGCCCTCAGCCCCCTGGACCCCGAGACCACCCGCTCCTCGGAGCTCACCCTC
ACCCCCAGGCCCGAGGACCATGGCACCAACCTCACCTGTCAGATGAAACGCCAAGGAGCT
CAGGTGACCACGGAGAGAACTGTCCAGCTCAATGTCTCCTATGCACCACAGACCATCACC
ATCTTCAGGAACGGCATAGCCCTAGAGATCCTGCAAAACACCTCATACCTTCCGGTCCTG
GAGGGCCAGGCTCTGCGGCTGCTCTGTGATGCTCCCAGCAACCCCCCTGCACACCTGAGC
TGGTTCCAGGGCTCCCCTGCCCTGAACGCCACCCCCATCTCCAATACCGGGATCTTGGAG
CTTCGTCGAGTAAGGTCTGCAGAAGAAGGAGGCTTCACCTGCCGCGCTCAGCACCCGCTG
GGCTTCCTGCAAATTTTTCTGAATCTCTCAGTTTACTCCCTCCCACAGTTGCTGGGCCCC
TCCTGCTCCTGGGAGGCTGAGGGTCTGCACTGCAGATGCTCCTTTCGAGCCCGGCCGGCC
CCCTCCCTGTGCTGGCGGCTTGAGGAGAAGCCGCTGGAGGGGAACAGCAGCCAGGGCTCA
TTCAAGGTCAACTCCAGCTCAGCTGGGCCCTGGGCCAACAGCTCCCTGATCCTCCACGGG
GGGCTCAGCTCCGACCTCAAAGTCAGCTGCAAGGCCTGGAACATCTATGGGTCCCAGAGC
GGCTCTGTCCTGCTGCTGCAAGGGAGATCGAACCTTGGGACAGGAGTGGTTCCTGCAGCC
CTTGGTGGTGCTGGTGTCATGGCCCTGCTCTGTATCTGTCTGTGCCTCATCTTCTTTTTA
ATAGTGAAAGCCCGCAGGAAGCAAGCAGCTGGGAGACCAGAGAAAATGGATGATGAAGAC
CCCATTATGGGTACCATCACCTCGGGTTCCAGGAAGAAGCCCTGGCCAGACAGCGCCGGA
GATCAAGCATCTCCTCCTGGGGATGCCCCTCCCTTGGAAGAACAAAAGGAGCTCCATTAT
GCCTCCCTTAGTTTTTCTGAGATGAAGTCGAGGGAGCCTAAGGACCAGGAGGCCCCAAGC
ACCACGGAGTACTCGGAGATCAAGACAAGCAAG

Protein sequence predicted based on nucleotide sequence

MLPLLLLPLLWGGSLQEKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYW
FRDGEIPYYAEVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRV
ERGRDVKYSYQQNKLNLEVTALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSW
TGNALSPLDPETTRSSELTLTPRPEDHGTNLTCQMKRQGAQVTTERTVQLNVSYAPQTIT
IFRNGIALEILQNTSYLPVLEGQALRLLCDAPSNPPAHLSWFQGSPALNATPISNTGILE
LRRVRSAEEGGFTCRAQHPLGFLQIFLNLSVYSLPQLLGPSCSWEAEGLHCRCSFRARPA
PSLCWRLEEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLSSDLKVSCKAWNIYGSQS
GSVLLLQGRSNLGTGVVPAALGGAGVMALLCICLCLIFFLIVKARRKQAAGRPEKMDDED
PIMGTITSGSRKKPWPDSAGDQASPPGDAPPLEEQKELHYASLSFSEMKSREPKDQEAPS
TTEYSEIKTSK

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

SIGLEC5

Symbol Nomenclature :

SIGLEC5

SYNONYM :

CD170; CD33L2; OB-BP2; OBBP2; SIGLEC-5

Designation :

CD33 antigen-like 2|OB-binding protein 2|obesity-binding protein 2|sialic acid-binding Ig-like lectin 5|sialic acid-binding immunoglobulin-like lectin 5

Full Nomenclature :

sialic acid binding Ig-like lectin 5

GENEID :

8778

GenBank Accession :

DQ892433

HGNC :

10874

MIM :

604200

Target GenBank:

DQ892433

Reference Sequence Alignment

  

HsCD00513343      ATGCTGCCCCTGCTGCTGCTGCCCCTGCTGTGGGGGGGGTCCCTGCAGGAGAAGCCAGTG
NM_003830.3       ATGCTGCCCCTGCTGCTGCTGCCCCTGCTGTGGGGGGGGTCCCTGCAGGAGAAGCCAGTG
                  ************************************************************

HsCD00513343      TACGAGCTGCAAGTGCAGAAGTCGGTGACGGTGCAGGAGGGCCTGTGCGTCCTTGTGCCC
NM_003830.3       TACGAGCTGCAAGTGCAGAAGTCGGTGACGGTGCAGGAGGGCCTGTGCGTCCTTGTGCCC
                  ************************************************************

HsCD00513343      TGCTCCTTCTCTTACCCCTGGAGATCCTGGTATTCCTCTCCCCCACTCTACGTCTACTGG
NM_003830.3       TGCTCCTTCTCTTACCCCTGGAGATCCTGGTATTCCTCTCCCCCACTCTACGTCTACTGG
                  ************************************************************

HsCD00513343      TTCCGGGACGGGGAGATCCCATACTACGCTGAGGTTGTGGCCACAAACAACCCAGACAGA
NM_003830.3       TTCCGGGACGGGGAGATCCCATACTACGCTGAGGTTGTGGCCACAAACAACCCAGACAGA
                  ************************************************************

HsCD00513343      AGAGTGAAGCCAGAGACCCAGGGCCGATTCCGCCTCCTTGGGGATGTCCAGAAGAAGAAC
NM_003830.3       AGAGTGAAGCCAGAGACCCAGGGCCGATTCCGCCTCCTTGGGGATGTCCAGAAGAAGAAC
                  ************************************************************

HsCD00513343      TGCTCCCTGAGCATCGGAGATGCCAGAATGGAGGACACGGGAAGCTATTTCTTCCGCGTG
NM_003830.3       TGCTCCCTGAGCATCGGAGATGCCAGAATGGAGGACACGGGAAGCTATTTCTTCCGCGTG
                  ************************************************************

HsCD00513343      GAGAGAGGAAGGGATGTAAAATATAGCTACCAACAGAATAAGCTGAACTTGGAGGTGACA
NM_003830.3       GAGAGAGGAAGGGATGTAAAATATAGCTACCAACAGAATAAGCTGAACTTGGAGGTGACA
                  ************************************************************

HsCD00513343      GCCCTGATAGAGAAACCCGACATCCACTTTCTGGAGCCTCTGGAGTCCGGCCGCCCCACA
NM_003830.3       GCCCTGATAGAGAAACCCGACATCCACTTTCTGGAGCCTCTGGAGTCCGGCCGCCCCACA
                  ************************************************************

HsCD00513343      AGGCTGAGCTGCAGCCTTCCAGGATCCTGTGAAGCGGGACCACCTCTCACATTCTCCTGG
NM_003830.3       AGGCTGAGCTGCAGCCTTCCAGGATCCTGTGAAGCGGGACCACCTCTCACATTCTCCTGG
                  ************************************************************

HsCD00513343      ACGGGGAATGCCCTCAGCCCCCTGGACCCCGAGACCACCCGCTCCTCGGAGCTCACCCTC
NM_003830.3       ACGGGGAATGCCCTCAGCCCCCTGGACCCCGAGACCACCCGCTCCTCGGAGCTCACCCTC
                  ************************************************************

HsCD00513343      ACCCCCAGGCCCGAGGACCATGGCACCAACCTCACCTGTCAGATGAAACGCCAAGGAGCT
NM_003830.3       ACCCCCAGGCCCGAGGACCATGGCACCAACCTCACCTGTCAGATGAAACGCCAAGGAGCT
                  ************************************************************

HsCD00513343      CAGGTGACCACGGAGAGAACTGTCCAGCTCAATGTCTCCTATGCACCACAGACCATCACC
NM_003830.3       CAGGTGACCACGGAGAGAACTGTCCAGCTCAATGTCTCCTATGCTCCACAGACCATCACC
                  ********************************************:***************

HsCD00513343      ATCTTCAGGAACGGCATAGCCCTAGAGATCCTGCAAAACACCTCATACCTTCCGGTCCTG
NM_003830.3       ATCTTCAGGAACGGCATAGCCCTAGAGATCCTGCAAAACACCTCATACCTTCCGGTCCTG
                  ************************************************************

HsCD00513343      GAGGGCCAGGCTCTGCGGCTGCTCTGTGATGCTCCCAGCAACCCCCCTGCACACCTGAGC
NM_003830.3       GAGGGCCAGGCTCTGCGGCTGCTCTGTGATGCTCCCAGCAACCCCCCTGCACACCTGAGC
                  ************************************************************

HsCD00513343      TGGTTCCAGGGCTCCCCTGCCCTGAACGCCACCCCCATCTCCAATACCGGGATCTTGGAG
NM_003830.3       TGGTTCCAGGGCTCCCCTGCCCTGAACGCCACCCCCATCTCCAATACCGGGATCTTGGAG
                  ************************************************************

HsCD00513343      CTTCGTCGAGTAAGGTCTGCAGAAGAAGGAGGCTTCACCTGCCGCGCTCAGCACCCGCTG
NM_003830.3       CTTCGTCGAGTAAGGTCTGCAGAAGAAGGAGGCTTCACCTGCCGCGCTCAGCACCCGCTG
                  ************************************************************

HsCD00513343      GGCTTCCTGCAAATTTTTCTGAATCTCTCAGTTTACTCCCTCCCACAGTTGCTGGGCCCC
NM_003830.3       GGCTTCCTGCAAATTTTTCTGAATCTCTCAGTTTACTCCCTCCCACAGTTGCTGGGCCCC
                  ************************************************************

HsCD00513343      TCCTGCTCCTGGGAGGCTGAGGGTCTGCACTGCAGATGCTCCTTTCGAGCCCGGCCGGCC
NM_003830.3       TCCTGCTCCTGGGAGGCTGAGGGTCTGCACTGCAGATGCTCCTTTCGAGCCCGGCCGGCC
                  ************************************************************

HsCD00513343      CCCTCCCTGTGCTGGCGGCTTGAGGAGAAGCCGCTGGAGGGGAACAGCAGCCAGGGCTCA
NM_003830.3       CCCTCCCTGTGCTGGCGGCTTGAGGAGAAGCCGCTGGAGGGGAACAGCAGCCAGGGCTCA
                  ************************************************************

HsCD00513343      TTCAAGGTCAACTCCAGCTCAGCTGGGCCCTGGGCCAACAGCTCCCTGATCCTCCACGGG
NM_003830.3       TTCAAGGTCAACTCCAGCTCAGCTGGGCCCTGGGCCAACAGCTCCCTGATCCTCCACGGG
                  ************************************************************

HsCD00513343      GGGCTCAGCTCCGACCTCAAAGTCAGCTGCAAGGCCTGGAACATCTATGGGTCCCAGAGC
NM_003830.3       GGGCTCAGCTCCGACCTCAAAGTCAGCTGCAAGGCCTGGAACATCTATGGGTCCCAGAGC
                  ************************************************************

HsCD00513343      GGCTCTGTCCTGCTGCTGCAAGGGAGATCGAACCTTGGGACAGGAGTGGTTCCTGCAGCC
NM_003830.3       GGCTCTGTCCTGCTGCTGCAAGGGAGATCGAACCTCGGGACAGGAGTGGTTCCTGCAGCC
                  *********************************** ************************

HsCD00513343      CTTGGTGGTGCTGGTGTCATGGCCCTGCTCTGTATCTGTCTGTGCCTCATCTTCTTTTTA
NM_003830.3       CTTGGTGGTGCTGGTGTCATGGCCCTGCTCTGTATCTGTCTGTGCCTCATCTTCTTTTTA
                  ************************************************************

HsCD00513343      ATAGTGAAAGCCCGCAGGAAGCAAGCAGCTGGGAGACCAGAGAAAATGGATGATGAAGAC
NM_003830.3       ATAGTGAAAGCCCGCAGGAAGCAAGCAGCTGGGAGACCAGAGAAAATGGATGATGAAGAC
                  ************************************************************

HsCD00513343      CCCATTATGGGTACCATCACCTCGGGTTCCAGGAAGAAGCCCTGGCCAGACAGCGCCGGA
NM_003830.3       CCCATTATGGGTACCATCACCTCGGGTTCCAGGAAGAAGCCCTGGCCAGACAGCCCCGGA
                  ****************************************************** *****

HsCD00513343      GATCAAGCATCTCCTCCTGGGGATGCCCCTCCCTTGGAAGAACAAAAGGAGCTCCATTAT
NM_003830.3       GATCAAGCATCTCCTCCTGGGGATGCCCCTCCCTTGGAAGAACAAAAGGAGCTCCATTAT
                  ************************************************************

HsCD00513343      GCCTCCCTTAGTTTTTCTGAGATGAAGTCGAGGGAGCCTAAGGACCAGGAGGCCCCAAGC
NM_003830.3       GCCTCCCTTAGTTTTTCTGAGATGAAGTCGAGGGAGCCTAAGGACCAGGAGGCCCCAAGC
                  ************************************************************

HsCD00513343      ACCACGGAGTACTCGGAGATCAAGACAAGCAAG---
NM_003830.3       ACCACGGAGTACTCGGAGATCAAGACAAGCAAGTGA
                  *********************************