DNASU Plasmid | ZNF404 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF404

Gene Name: zinc finger protein 404

Original Clone ID:

ccsbBroadEn_10039

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC101333.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513371

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1657nts

Open reading frame: 1 to 1657

Reference Sequence Alignment

ATGGCCCGGGTGCCATTGACATTCAGCGATGTTGCCATAGACTTCTCTCAGGAGGAGTGG
GAATATTTAAACTCGGATCAGAGGGATTTGTACAGAGATGTGATGTTGGAGAATTATACT
AACTTGGTCTCATTGGACTTTAATTTCACAACTGAAAGCAACAAGTTATCTTCAGAAAAA
AGAAATTATGAAGTAAATGCGTACCATCAGGAGACATGGAAAAGAAATAAAACCTTCAAC
CTTATGAGGTTTATTTTCAGAACTGACCCACAGTACACAATTGAATTTGGGAGACAACAG
AGACCTAAAGTGGGATGTTTTAGTCAAATGATATTCAAAAAACATAAATCCCTTCCTCTA
CATAAGAGAAATAACACAAGAGAGAAATCATATGAGTGTAAGGAATATAAGAAGGGCTTT
AGAAAATATTTGCACCTTACTGAACATCTGAGAGACCATACTGGTGTGATACCCTATGAA
TGTAATGAATGTGGAAAAGCATTTGTAGTTTTCCAGCATTTTATTAGACATCGAAAAATC
CACACTGATTTGAAACCCTATGAATGCAATGGATGTGAGAAGGCCTTTAGGTTTTATTCA
CAGCTTATTCAGCATCAGATAATTCATACTGGTATGAAACCCTATGAATGTAAGCAATGC
GGGAAGGCTTTTAGACGTCATTCTCACCTTACAGAACATCAGAAAATTCATGTTGGCTTG
AAACCCTTTGAATGTAAGGAATGTGGGGAAACGTTTAGATTATATCGACATATGTGTCTG
CATCAGAAAATTCATCATGGTGTGAAACCCTACAAATGTAAAGAATGTGGAAAGGCTTTT
GGTCATCGTTCAAGTCTTTACCAACATAAGAAAATTCATTCTGGTGAGAAACCATATAAA
TGTGAACAATGTGAAAAGGCCTTTGTTCGCAGCTATCTACTTGTTGAACATCAAAGAAGT
CATACTGGTGAGAAACCTCATGAATGCATGGAATGTGGAAAGGCTTTTAGTAAGGGCTCA
AGCCTTCTTAAACATAAGAGAATTCATAGTAGTGAGAAACTCTATGATTGTAAGGATTGT
GGAAAGGCCTTTTGTAGAGGCTCTCAACTTACACAGCATCAGAGAATTCATACTGGTGAG
AAGCCACATGAATGTAAAGAATGTGGGAAGACTTTTAAGCTTCATTCATATCTTATTCAA
CATCAGATAATTCATACTGATTTGAAGCCATATGAATGTAAGCAATGTGGGAAAGCCTTC
AGTCGTGTTGGAGACCTTAAGACACATCAATCAATTCATGCTGGGGAGAAACCCTATGAA
TGTAAGGAATGTGGAAAAACCTTTAGACTTAATTCTCAACTAATTTATCATCAGACAATT
CATACTGGTTTGAAACCCTATGTATGTAAAGAATGTAAGAAGGCCTTTCGTTCTATCTCA
GGTCTTTCTCAACATAAGAGAATTCATACTGGTGAAAAACCCTATGAATGTAAAGAATGT
GATAAGGCCTTTAATCGCAGTGATCGACTTACTCAACATGAGACAATTCATACTGGTGTG
AAACCACAGAAATGCAAAGAATGTGGTAAGGCCTTTAGTCATTGCTATCAACTTAGTCAA
CATCAAAGATTTCACCATGGTGAGAGACTCTTAATG

Protein sequence predicted based on nucleotide sequence

MARVPLTFSDVAIDFSQEEWEYLNSDQRDLYRDVMLENYTNLVSLDFNFTTESNKLSSEK
RNYEVNAYHQETWKRNKTFNLMRFIFRTDPQYTIEFGRQQRPKVGCFSQMIFKKHKSLPL
HKRNNTREKSYECKEYKKGFRKYLHLTEHLRDHTGVIPYECNECGKAFVVFQHFIRHRKI
HTDLKPYECNGCEKAFRFYSQLIQHQIIHTGMKPYECKQCGKAFRRHSHLTEHQKIHVGL
KPFECKECGETFRLYRHMCLHQKIHHGVKPYKCKECGKAFGHRSSLYQHKKIHSGEKPYK
CEQCEKAFVRSYLLVEHQRSHTGEKPHECMECGKAFSKGSSLLKHKRIHSSEKLYDCKDC
GKAFCRGSQLTQHQRIHTGEKPHECKECGKTFKLHSYLIQHQIIHTDLKPYECKQCGKAF
SRVGDLKTHQSIHAGEKPYECKECGKTFRLNSQLIYHQTIHTGLKPYVCKECKKAFRSIS
GLSQHKRIHTGEKPYECKECDKAFNRSDRLTQHETIHTGVKPQKCKECGKAFSHCYQLSQ
HQRFHHGERLLM

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF404

Symbol Nomenclature :

ZNF404

Full Nomenclature :

zinc finger protein 404

GENEID :

342908

HGNC :

19417

Vega :

OTTHUMG00000182206

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00513371        ATGGCCCGGGTGCCATTGACATTCAGCGATGTTGCCATAGACTTCTCTCAGGAGGAGTGG
NM_001033719.2      ATGGCCCGGGTGCCATTGACATTCAGCGATGTTGCCATAGACTTCTCTCAGGAGGAGTGG
                    ************************************************************

HsCD00513371        GAATATTTAAACTCGGATCAGAGGGATTTGTACAGAGATGTGATGTTGGAGAATTATACT
NM_001033719.2      GAATATTTAAACTCGGATCAGAGGGATTTGTACAGAGATGTGATGTTGGAGAATTATACT
                    ************************************************************

HsCD00513371        AACTTGGTCTCATTGGACTTTAATTTCACAACTGAAAGCAACAAGTTATCTTCAGAAAAA
NM_001033719.2      AACTTGGTCTCATTGGACTTTAATTTCACAACTGAAAGCAACAAGTTATCTTCAGAAAAA
                    ************************************************************

HsCD00513371        AGAAATTATGAAGTAAATGCGTACCATCAGGAGACATGGAAAAGAAATAAAACCTTCAAC
NM_001033719.2      AGAAATTATGAAGTAAATGCGTACCATCAGGAGACATGGAAAAGAAATAAAACCTTCAAC
                    ************************************************************

HsCD00513371        CTTATGAGGTTTATTTTCAGAACTGACCCACAGTACACAATTGAATTTGGGAGACAACAG
NM_001033719.2      CTTATGAGGTTTATTTTCAGAACTGACCCACAGTACACAATTGAATTTGGGAGACAACAG
                    ************************************************************

HsCD00513371        AGACCTAAAGTGGGATGTTTTAGTCAAATGATATTCAAAAAACATAAATCCCTTCCTCTA
NM_001033719.2      AGACCTAAAGTGGGATGTTTTAGTCAAATGATATTCAAAAAACATAAATCCCTTCCTCTA
                    ************************************************************

HsCD00513371        CATAAGAGAAATAACACAAGAGAGAAATCATATGAGTGTAAGGAATATAAGAAGGGCTTT
NM_001033719.2      CATAAGAGAAATAACACAAGAGAGAAATCATATGAGTGTAAGGAATATAAGAAGGGCTTT
                    ************************************************************

HsCD00513371        AGAAAATATTTGCACCTTACTGAACATCTGAGAGACCATACTGGTGTGATACCCTATGAA
NM_001033719.2      AGAAAATATTTGCACCTTACTGAACATCTGAGAGACCATACTGGTGTGATACCCTATGAA
                    ************************************************************

HsCD00513371        TGTAATGAATGTGGAAAAGCATTTGTAGTTTTCCAGCATTTTATTAGACATCGAAAAATC
NM_001033719.2      TGTAATGAATGTGGAAAAGCATTTGTAGTTTTCCAGCATTTTATTAGACATCGAAAAATC
                    ************************************************************

HsCD00513371        CACACTGATTTGAAACCCTATGAATGCAATGGATGTGAGAAGGCCTTTAGGTTTTATTCA
NM_001033719.2      CACACTGATTTGAAACCCTATGAATGCAATGGATGTGAGAAGGCCTTTAGGTTTTATTCA
                    ************************************************************

HsCD00513371        CAGCTTATTCAGCATCAGATAATTCATACTGGTATGAAACCCTATGAATGTAAGCAATGC
NM_001033719.2      CAGCTTATTCAGCATCAGATAATTCATACTGGTATGAAACCCTATGAATGTAAGCAATGC
                    ************************************************************

HsCD00513371        GGGAAGGCTTTTAGACGTCATTCTCACCTTACAGAACATCAGAAAATTCATGTTGGCTTG
NM_001033719.2      GGGAAGGCTTTTAGACGTCATTCTCACCTTACAGAACATCAGAAAATTCATGTTGGCTTG
                    ************************************************************

HsCD00513371        AAACCCTTTGAATGTAAGGAATGTGGGGAAACGTTTAGATTATATCGACATATGTGTCTG
NM_001033719.2      AAACCCTTTGAATGTAAGGAATGTGGGGAAACGTTTAGATTATATCGACATATGTGTCTG
                    ************************************************************

HsCD00513371        CATCAGAAAATTCATCATGGTGTGAAACCCTACAAATGTAAAGAATGTGGAAAGGCTTTT
NM_001033719.2      CATCAGAAAATTCATCATGGTGTGAAACCCTACAAATGTAAAGAATGTGGAAAGGCTTTT
                    ************************************************************

HsCD00513371        GGTCATCGTTCAAGTCTTTACCAACATAAGAAAATTCATTCTGGTGAGAAACCATATAAA
NM_001033719.2      GGTCATCGTTCAAGTCTTTACCAACATAAGAAAATTCATTCTGGTGAGAAACCATATAAA
                    ************************************************************

HsCD00513371        TGTGAACAATGTGAAAAGGCCTTTGTTCGCAGCTATCTACTTGTTGAACATCAAAGAAGT
NM_001033719.2      TGTGAACAATGTGAAAAGGCCTTTGTTCGCAGCTATCTACTTGTTGAACATCAAAGAAGT
                    ************************************************************

HsCD00513371        CATACTGGTGAGAAACCTCATGAATGCATGGAATGTGGAAAGGCTTTTAGTAAGGGCTCA
NM_001033719.2      CATACTGGTGAGAAACCTCATGAATGCATGGAATGTGGAAAGGCTTTTGGTAAGGGCTCA
                    ************************************************.***********

HsCD00513371        AGCCTTCTTAAACATAAGAGAATTCATAGTAGTGAGAAACTCTATGATTGTAAGGATTGT
NM_001033719.2      AGCCTTCTTAAACATAAGAGAATTCATAGTAGTGAGAAACTCTATGATTGTAAGGATTGT
                    ************************************************************

HsCD00513371        GGAAAGGCCTTTTGTAGAGGCTCTCAACTTACACAGCATCAGAGAATTCATACTGGTGAG
NM_001033719.2      GGAAAGGCCTTTTGTAGAGGCTCTCAACTTACACAGCATCAGAGAATTCATACTGGTGAG
                    ************************************************************

HsCD00513371        AAGCCACATGAATGTAAAGAATGTGGGAAGACTTTTAAGCTTCATTCATATCTTATTCAA
NM_001033719.2      AAGCCACATGAATGTAAAGAATGTGGGAAGACTTTTAAGCTTCATTCATATCTTATTCAA
                    ************************************************************

HsCD00513371        CATCAGATAATTCATACTGATTTGAAGCCATATGAATGTAAGCAATGTGGGAAAGCCTTC
NM_001033719.2      CATCAGATAATTCATACTGATTTGAAGCCATATGAATGTAAGCAATGTGGGAAAGCCTTC
                    ************************************************************

HsCD00513371        AGTCGTGTTGGAGACCTTAAGACACATCAATCAATTCATGCTGGGGAGAAACCCTATGAA
NM_001033719.2      AGTCGTGTTGGAGACCTTAAGACACATCAATCAATTCATGCTGGGGAGAAACCCTATGAA
                    ************************************************************

HsCD00513371        TGTAAGGAATGTGGAAAAACCTTTAGACTTAATTCTCAACTAATTTATCATCAGACAATT
NM_001033719.2      TGTAAGGAATGTGGAAAAACCTTTAGACTTAATTCTCAACTAATTTATCATCAGACAATT
                    ************************************************************

HsCD00513371        CATACTGGTTTGAAACCCTATGTATGTAAAGAATGTAAGAAGGCCTTTCGTTCTATCTCA
NM_001033719.2      CATACTGGTTTGAAACCCTATGTATGTAAAGAATGTAAGAAGGCCTTTCGTTCTATCTCA
                    ************************************************************

HsCD00513371        GGTCTTTCTCAACATAAGAGAATTCATACTGGTGAAAAACCCTATGAATGTAAAGAATGT
NM_001033719.2      GGTCTTTCTCAACATAAGAGAATTCATACTGGTGAAAAACCCTATGAATGTAAAGAATGT
                    ************************************************************

HsCD00513371        GATAAGGCCTTTAATCGCAGTGATCGACTTACTCAACATGAGACAATTCATACTGGTGTG
NM_001033719.2      GATAAGGCCTTTAATCGCAGTGATCGACTTACTCAACATGAGACAATTCATACTGGTGTG
                    ************************************************************

HsCD00513371        AAACCACAGAAATGCAAAGAATGTGGTAAGGCCTTTAGTCATTGCTATCAACTTAGTCAA
NM_001033719.2      AAACCACAGAAATGCAAAGAATGTGGTAAGGCCTTTAGTCATTGCTATCAACTTAGTCAA
                    ************************************************************

HsCD00513371        CATCAAAGATTTCACCATGGTGAGAGACTCTTAATG---
NM_001033719.2      CATCAAAGATTTCACCATGGTGAGAGACTCTTAATGTAA
                    ************************************