DNASU Plasmid | ZNF8 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF8

Gene Name: zinc finger protein 8

Original Clone ID:

ccsbBroadEn_07147

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC039323.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513398

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1726nts

Open reading frame: 1 to 1726

Reference Sequence Alignment

ATGGACCCCGAGGACGAAGGGGTAGCGGGAGTGATGTCTGTGGGGCCGCCGGCGGCCCGG
CTTCAGGAACCAGTGACCTTCCGGGATGTGGCTGTGGACTTTACCCAGGAGGAATGGGGG
CAGCTGGACCCTACCCAGAGGATCCTCTACCGTGACGTGATGCTGGAGACCTTTGGTCAC
CTGCTCTCCATAGGTCCTGAGCTTCCGAAGCCTGAAGTCATCTCCCAGCTGGAGCAAGGG
ACCGAGCTATGGGTGGCTGAGAGAGGAACCACCCAGGGCTGCCATCCAGCCTGGGAGCCT
CGATCTGAAAGCCAAGCATCACGCAAGGAAGAGGGCCTGCCTGAAGAGGAGCCATCCCAT
GTCACGGGAAGGGAAGGATTCCCGACAGATGCTCCTTATCCCACCACGTTAGGGAAAGAC
AGGGAGTGTCAGAGCCAGAGTCTGGCACTCAAGGAGCAGAATAACTTGAAGCAGTTGGAA
TTTGGCCTCAAGGAAGCACCAGTTCAAGATCAAGGCTACAAAACTCTCAGACTCAGGGAA
AACTGCGTCCTGAGTTCAAGCCCAAATCCATTCCCAGAGATCTCTAGAGGGGAGTATTTG
TATACTTACGACTCACAGATTACAGACTCAGAACATAACTCCAGCTTAGTCAGTCAGCAG
ACAGGCTCCCCAGGAAAACAGCCCGGTGAAAACAGTGACTGTCACAGAGATTCCAGTCAG
GCCATTCCAATTACGGAACTCACAAAAAGCCAGGTGCAGGACAAACCCTACAAATGTACT
GACTGTGGGAAGTCGTTTAACCATAACGCACACCTCACCGTGCACAAGAGGATTCATACG
GGAGAAAGACCTTATATGTGCAAGGAGTGTGGGAAAGCCTTCAGCCAGAACTCCTCCCTC
GTCCAGCATGAGCGCATCCACACTGGAGACAAGCCCTACAAGTGTGCCGAATGTGGGAAG
TCTTTCTGCCATAGTACACACCTTACCGTCCATCGGAGGATTCACACTGGGGAGAAGCCC
TATGAGTGTCAGGACTGTGGGAGGGCCTTCAACCAGAACTCCTCCCTGGGGCGGCACAAG
AGGACACACACTGGGGAGAAGCCATACACCTGCAGTGTGTGTGGGAAATCCTTCTCTCGG
ACCACTTGCCTTTTCCTGCACCTGAGAACTCACACCGAGGAGAGGCCCTACGAGTGTAAC
CACTGCGGGAAGGGCTTCAGGCACAGCTCATCCCTGGCCCAGCACCAGCGGAAGCACGCG
GGGGAGAAGCCCTTTGAGTGCCGCCAGAGGCTGATCTTTGAGCAGACGCCAGCTCTCACA
AAGCATGAATGGACAGAAGCCCTGGGCTGTGACCCACCTTTGAGTCAAGATGAGAGGACT
CACCGAAGCGACAGACCCTTCAAATGTAATCAGTGTGGGAAGTGTTTCATTCAGAGCTCT
CACCTCATCCGGCACCAGATAACTCACACCAGAGAGGAGCAGCCCCATGGGCGAAGCCGG
CGGCGTGAACAGTCCTCGAGCAGGAACTCACACCTGGTTCAGCATCAACACCCGAACTCC
AGAAAGAGCTCTGCAGGCGGAGCAAAGGCAGGGCAGCCGGAAAGCAGAGCCCTGGCTTTG
TTTGACATCCAAAAAATCATGCAAGAGAAAAACCCTGTGCACGTTATTGGGGTGGAAGAG
CCTTCTGTGGGTGCTTCCATGTTATTTGACATCAGAGAATCCACA

Protein sequence predicted based on nucleotide sequence

MDPEDEGVAGVMSVGPPAARLQEPVTFRDVAVDFTQEEWGQLDPTQRILYRDVMLETFGH
LLSIGPELPKPEVISQLEQGTELWVAERGTTQGCHPAWEPRSESQASRKEEGLPEEEPSH
VTGREGFPTDAPYPTTLGKDRECQSQSLALKEQNNLKQLEFGLKEAPVQDQGYKTLRLRE
NCVLSSSPNPFPEISRGEYLYTYDSQITDSEHNSSLVSQQTGSPGKQPGENSDCHRDSSQ
AIPITELTKSQVQDKPYKCTDCGKSFNHNAHLTVHKRIHTGERPYMCKECGKAFSQNSSL
VQHERIHTGDKPYKCAECGKSFCHSTHLTVHRRIHTGEKPYECQDCGRAFNQNSSLGRHK
RTHTGEKPYTCSVCGKSFSRTTCLFLHLRTHTEERPYECNHCGKGFRHSSSLAQHQRKHA
GEKPFECRQRLIFEQTPALTKHEWTEALGCDPPLSQDERTHRSDRPFKCNQCGKCFIQSS
HLIRHQITHTREEQPHGRSRRREQSSSRNSHLVQHQHPNSRKSSAGGAKAGQPESRALAL
FDIQKIMQEKNPVHVIGVEEPSVGASMLFDIREST

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF8

Symbol Nomenclature :

ZNF8

SYNONYM :

HF.18; Zfp128

Designation :

zinc finger protein 272|zinc finger protein 8 (clone HF.18)|zinc finger protein HF.18

Full Nomenclature :

zinc finger protein 8

GENEID :

7554

GenBank Accession :

EU446717

HGNC :

13154

MIM :

194532

Vega :

OTTHUMG00000182073

Target GenBank:

EU446717

Reference Sequence Alignment

  

HsCD00513398      ATGGACCCCGAGGACGAAGGGGTAGCGGGAGTGATGTCTGTGGGGCCGCCGGCGGCCCGG
NM_021089.2       ATGGACCCCGAGGACGAAGGGGTAGCGGGAGTGATGTCTGTGGGGCCGCCGGCGGCCCGG
                  ************************************************************

HsCD00513398      CTTCAGGAACCAGTGACCTTCCGGGATGTGGCTGTGGACTTTACCCAGGAGGAATGGGGG
NM_021089.2       CTTCAGGAACCAGTGACCTTCCGGGATGTGGCTGTGGACTTTACCCAGGAGGAATGGGGG
                  ************************************************************

HsCD00513398      CAGCTGGACCCTACCCAGAGGATCCTCTACCGTGACGTGATGCTGGAGACCTTTGGTCAC
NM_021089.2       CAGCTGGACCCTACCCAGAGGATCCTCTACCGTGACGTGATGCTGGAGACCTTTGGTCAC
                  ************************************************************

HsCD00513398      CTGCTCTCCATAGGTCCTGAGCTTCCGAAGCCTGAAGTCATCTCCCAGCTGGAGCAAGGG
NM_021089.2       CTGCTCTCCATAGGTCCTGAGCTTCCGAAGCCTGAAGTCATCTCCCAGCTGGAGCAAGGG
                  ************************************************************

HsCD00513398      ACCGAGCTATGGGTGGCTGAGAGAGGAACCACCCAGGGCTGCCATCCAGCCTGGGAGCCT
NM_021089.2       ACCGAGCTATGGGTGGCTGAGAGAGGAACCACCCAGGGCTGCCATCCAGCCTGGGAGCCT
                  ************************************************************

HsCD00513398      CGATCTGAAAGCCAAGCATCACGCAAGGAAGAGGGCCTGCCTGAAGAGGAGCCATCCCAT
NM_021089.2       CGATCTGAAAGCCAAGCATCACGCAAGGAAGAGGGCCTGCCTGAAGAGGAGCCATCCCAT
                  ************************************************************

HsCD00513398      GTCACGGGAAGGGAAGGATTCCCGACAGATGCTCCTTATCCCACCACGTTAGGGAAAGAC
NM_021089.2       GTCACGGGAAGGGAAGGATTCCCGACAGATGCTCCTTATCCCACCACGTTAGGGAAAGAC
                  ************************************************************

HsCD00513398      AGGGAGTGTCAGAGCCAGAGTCTGGCACTCAAGGAGCAGAATAACTTGAAGCAGTTGGAA
NM_021089.2       AGGGAGTGTCAGAGCCAGAGTCTGGCACTCAAGGAGCAGAATAACTTGAAGCAGTTGGAA
                  ************************************************************

HsCD00513398      TTTGGCCTCAAGGAAGCACCAGTTCAAGATCAAGGCTACAAAACTCTCAGACTCAGGGAA
NM_021089.2       TTTGGCCTCAAGGAAGCACCAGTTCAAGATCAAGGCTACAAAACTCTCAGACTCAGGGAA
                  ************************************************************

HsCD00513398      AACTGCGTCCTGAGTTCAAGCCCAAATCCATTCCCAGAGATCTCTAGAGGGGAGTATTTG
NM_021089.2       AACTGCGTCCTGAGTTCAAGCCCAAATCCATTCCCAGAGATCTCTAGAGGGGAGTATTTG
                  ************************************************************

HsCD00513398      TATACTTACGACTCACAGATTACAGACTCAGAACATAACTCCAGCTTAGTCAGTCAGCAG
NM_021089.2       TATACTTACGACTCACAGATTACAGACTCAGAACATAACTCCAGCTTAGTCAGTCAGCAG
                  ************************************************************

HsCD00513398      ACAGGCTCCCCAGGAAAACAGCCCGGTGAAAACAGTGACTGTCACAGAGATTCCAGTCAG
NM_021089.2       ACAGGCTCCCCAGGAAAACAGCCCGGTGAAAACAGTGACTGTCACAGAGATTCCAGTCAG
                  ************************************************************

HsCD00513398      GCCATTCCAATTACGGAACTCACAAAAAGCCAGGTGCAGGACAAACCCTACAAATGTACT
NM_021089.2       GCCATTCCAATTACGGAACTCACAAAAAGCCAGGTGCAGGACAAACCCTACAAATGTACT
                  ************************************************************

HsCD00513398      GACTGTGGGAAGTCGTTTAACCATAACGCACACCTCACCGTGCACAAGAGGATTCATACG
NM_021089.2       GACTGTGGGAAGTCGTTTAACCATAACGCACACCTCACCGTGCACAAGAGGATTCATACG
                  ************************************************************

HsCD00513398      GGAGAAAGACCTTATATGTGCAAGGAGTGTGGGAAAGCCTTCAGCCAGAACTCCTCCCTC
NM_021089.2       GGAGAAAGACCTTATATGTGCAAGGAGTGTGGGAAAGCCTTCAGCCAGAACTCCTCCCTC
                  ************************************************************

HsCD00513398      GTCCAGCATGAGCGCATCCACACTGGAGACAAGCCCTACAAGTGTGCCGAATGTGGGAAG
NM_021089.2       GTCCAGCATGAGCGCATCCACACTGGAGACAAGCCCTACAAGTGTGCCGAATGTGGGAAG
                  ************************************************************

HsCD00513398      TCTTTCTGCCATAGTACACACCTTACCGTCCATCGGAGGATTCACACTGGGGAGAAGCCC
NM_021089.2       TCTTTCTGCCATAGTACACACCTTACCGTCCATCGGAGGATTCACACTGGGGAGAAGCCC
                  ************************************************************

HsCD00513398      TATGAGTGTCAGGACTGTGGGAGGGCCTTCAACCAGAACTCCTCCCTGGGGCGGCACAAG
NM_021089.2       TATGAGTGTCAGGACTGTGGGAGGGCCTTCAACCAGAACTCCTCCCTGGGGCGGCACAAG
                  ************************************************************

HsCD00513398      AGGACACACACTGGGGAGAAGCCATACACCTGCAGTGTGTGTGGGAAATCCTTCTCTCGG
NM_021089.2       AGGACACACACTGGGGAGAAGCCATACACCTGCAGTGTGTGTGGGAAATCCTTCTCTCGG
                  ************************************************************

HsCD00513398      ACCACTTGCCTTTTCCTGCACCTGAGAACTCACACCGAGGAGAGGCCCTACGAGTGTAAC
NM_021089.2       ACCACTTGCCTTTTCCTGCACCTGAGAACTCACACCGAGGAGAGGCCCTACGAGTGTAAC
                  ************************************************************

HsCD00513398      CACTGCGGGAAGGGCTTCAGGCACAGCTCATCCCTGGCCCAGCACCAGCGGAAGCACGCG
NM_021089.2       CACTGCGGGAAGGGCTTCAGGCACAGCTCATCCCTGGCCCAGCACCAGCGGAAGCACGCG
                  ************************************************************

HsCD00513398      GGGGAGAAGCCCTTTGAGTGCCGCCAGAGGCTGATCTTTGAGCAGACGCCAGCTCTCACA
NM_021089.2       GGGGAGAAGCCCTTTGAGTGCCGCCAGAGGCTGATCTTTGAGCAGACGCCAGCTCTCACA
                  ************************************************************

HsCD00513398      AAGCATGAATGGACAGAAGCCCTGGGCTGTGACCCACCTTTGAGTCAAGATGAGAGGACT
NM_021089.2       AAGCATGAATGGACAGAAGCCCTGGGCTGTGACCCACCTTTGAGTCAAGATGAGAGGACT
                  ************************************************************

HsCD00513398      CACCGAAGCGACAGACCCTTCAAATGTAATCAGTGTGGGAAGTGTTTCATTCAGAGCTCT
NM_021089.2       CACCGAAGCGACAGACCCTTCAAATGTAATCAGTGTGGGAAGTGTTTCATTCAGAGCTCT
                  ************************************************************

HsCD00513398      CACCTCATCCGGCACCAGATAACTCACACCAGAGAGGAGCAGCCCCATGGGCGAAGCCGG
NM_021089.2       CACCTCATCCGGCACCAGATAACTCACACCAGAGAGGAGCAGCCCCATGGGCGAAGCCGG
                  ************************************************************

HsCD00513398      CGGCGTGAACAGTCCTCGAGCAGGAACTCACACCTGGTTCAGCATCAACACCCGAACTCC
NM_021089.2       CGGCGTGAACAATCCTCGAGCAGGAACTCACACCTGGTTCAGCATCAACACCCGAACTCC
                  ***********.************************************************

HsCD00513398      AGAAAGAGCTCTGCAGGCGGAGCAAAGGCAGGGCAGCCGGAAAGCAGAGCCCTGGCTTTG
NM_021089.2       AGAAAGAGCTCTGCAGGCGGAGCAAAGGCAGGGCAGCCGGAAAGCAGAGCCCTGGCTTTG
                  ************************************************************

HsCD00513398      TTTGACATCCAAAAAATCATGCAAGAGAAAAACCCTGTGCACGTTATTGGGGTGGAAGAG
NM_021089.2       TTTGACATCCAAAAAATCATGCAAGAGAAAAACCCTGTGCACGTTATTGGGGTGGAAGAG
                  ************************************************************

HsCD00513398      CCTTCTGTGGGTGCTTCCATGTTATTTGACATCAGAGAATCCACA---
NM_021089.2       CCTTCTGTGGGTGCTTCCATGTTATTTGACATCAGAGAATCCACATAG
                  *********************************************