DNASU Plasmid | ZNF543 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF543

Gene Name: zinc finger protein 543

Original Clone ID:

ccsbBroadEn_04801

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC101015.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513559

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1801nts

Open reading frame: 1 to 1801

Reference Sequence Alignment

ATGGCAGCCTCGGCGCAGGTGTCTGTGACCTTTGAGGATGTGGCTGTGACATTCACCCAG
GAGGAGTGGGGACAGTTGGATGCAGCCCAGAGAACCTTGTATCAGGAGGTGATGCTGGAG
ACCTGCGGACTTCTCATGTCTCTGGGCTGTCCTTTGTTCAAACCAGAGCTGATCTACCAG
TTGGATCACAGACAGGAGCTATGGATGGCTACAAAAGACCTCTCCCAAAGCTCCTATCCA
GGTGACAACACAAAACCCAAGACCACAGAGCCTACCTTTTCTCACCTGGCCTTGCCTGAG
GAAGTCTTACTCCAGGAACAACTGACACAAGGAGCCTCAAAGAACTCCCAATTAGGGCAA
TCCAAGGATCAGGATGGGCCATCTGAAATGCAAGAAGTCCACTTGAAAATAGGGATAGGC
CCCCAGCGGGGGAAGCTGCTGGAGAAAATGAGTTCTGAACGTGATGGTTTGGGGTCAGAT
GATGGTGTATGTACAAAGATTACACAGAAACAAGTTTCAACAGAAGGTGATCTCTATGAA
TGTGATTCACATGGACCAGTTACAGATGCCTTGATTCGCGAAGAGAAAAATTCCTATAAA
TGTGAGGAATGCGGGAAAGTGTTTAAAAAGAATGCCCTCCTTGTTCAGCATGAACGGATT
CACACTCAAGTGAAGCCCTATGAATGCACAGAGTGTGGGAAAACCTTTAGCAAGAGCACT
CATCTTCTTCAGCACCTCATCATCCACACTGGGGAGAAGCCCTATAAGTGCATGGAGTGT
GGGAAGGCTTTTAACCGCAGGTCACACCTCACACGGCACCAGCGGATTCACAGTGGAGAG
AAGCCTTATAAGTGCAGTGAATGTGGAAAGGCCTTCACCCACCGCTCCACTTTTGTCTTG
CATCACAGGAGCCACACTGGAGAAAAACCCTTTGTGTGCAAAGAGTGTGGCAAAGCCTTT
CGAGATAGGCCAGGTTTCATTCGACACTACATCATCCACACGGGAGAGAAGCCCTATGAG
TGCATTGAGTGTGGGAAGGCCTTCAACCGCCGGTCATACCTCACGTGGCACCAACAGATT
CACACTGGAGTGAAACCCTTTGAATGCAACGAGTGTGGAAAAGCTTTTTGCGAGAGTGCA
GACCTCATTCAACACTACATTATCCACACTGGGGAGAAGCCCTATAAGTGCATGGAGTGT
GGGAAGGCGTTCAACCGTAGGTCACACCTCAAGCAGCATCAACGGATTCACACTGGGGAG
AAGCCTTATGAATGCAGTGAATGTGGAAAGGCCTTCACCCACTGCTCCACTTTTGTCTTG
CATAAAAGGACCCACACAGGAGAAAAACCCTATGAATGCAAAGAATGTGGAAAAGCCTTT
AGTGATAGGGCAGACCTCATTCGCCACTTCAGCATCCACACTGGAGAGAAACCCTATGAG
TGCGTGGAGTGTGGAAAGGCCTTCAACCGCAGCTCACACCTCACGAGGCACCAACAGATT
CACACTGGAGAGAAACCCTATGAATGCATCCAGTGTGGGAAAGCCTTTTGCCGGAGCGCA
AACCTTATTCGACACTCCATCATTCACACTGGAGAGAAGCCGTATGAATGCAGTGAGTGT
GGAAAGGCTTTTAATCGCGGCTCATCCCTCACACATCATCAAAGGATTCATACTGGGAGA
AACCCTACCATTGTAACAGATGTGGGAAGACCTTTTATGACTGCACAGACTTCAGTCAAC
ATCCAGGAACTTTTATTAGGGAAAGAGTTTTTGAATATCACCACTGAAGAAAATCTGTGG

Protein sequence predicted based on nucleotide sequence

MAASAQVSVTFEDVAVTFTQEEWGQLDAAQRTLYQEVMLETCGLLMSLGCPLFKPELIYQ
LDHRQELWMATKDLSQSSYPGDNTKPKTTEPTFSHLALPEEVLLQEQLTQGASKNSQLGQ
SKDQDGPSEMQEVHLKIGIGPQRGKLLEKMSSERDGLGSDDGVCTKITQKQVSTEGDLYE
CDSHGPVTDALIREEKNSYKCEECGKVFKKNALLVQHERIHTQVKPYECTECGKTFSKST
HLLQHLIIHTGEKPYKCMECGKAFNRRSHLTRHQRIHSGEKPYKCSECGKAFTHRSTFVL
HHRSHTGEKPFVCKECGKAFRDRPGFIRHYIIHTGEKPYECIECGKAFNRRSYLTWHQQI
HTGVKPFECNECGKAFCESADLIQHYIIHTGEKPYKCMECGKAFNRRSHLKQHQRIHTGE
KPYECSECGKAFTHCSTFVLHKRTHTGEKPYECKECGKAFSDRADLIRHFSIHTGEKPYE
CVECGKAFNRSSHLTRHQQIHTGEKPYECIQCGKAFCRSANLIRHSIIHTGEKPYECSEC
GKAFNRGSSLTHHQRIHTGRNPTIVTDVGRPFMTAQTSVNIQELLLGKEFLNITTEENLW

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF543

Symbol Nomenclature :

ZNF543

Full Nomenclature :

zinc finger protein 543

GENEID :

125919

HGNC :

25281

Vega :

OTTHUMG00000183243

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00513559      ATGGCAGCCTCGGCGCAGGTGTCTGTGACCTTTGAGGATGTGGCTGTGACATTCACCCAG
NM_213598.3       ATGGCAGCCTCGGCGCAGGTGTCTGTGACCTTTGAGGATGTGGCTGTGACATTCACCCAG
                  ************************************************************

HsCD00513559      GAGGAGTGGGGACAGTTGGATGCAGCCCAGAGAACCTTGTATCAGGAGGTGATGCTGGAG
NM_213598.3       GAGGAGTGGGGACAGTTGGATGCAGCCCAGAGAACCTTGTATCAGGAGGTGATGCTGGAG
                  ************************************************************

HsCD00513559      ACCTGCGGACTTCTCATGTCTCTGGGCTGTCCTTTGTTCAAACCAGAGCTGATCTACCAG
NM_213598.3       ACCTGCGGACTTCTCATGTCTCTGGGCTGTCCTTTGTTCAAACCAGAGCTGATCTACCAG
                  ************************************************************

HsCD00513559      TTGGATCACAGACAGGAGCTATGGATGGCTACAAAAGACCTCTCCCAAAGCTCCTATCCA
NM_213598.3       TTGGATCACAGACAGGAGCTATGGATGGCTACAAAAGACCTCTCCCAAAGCTCCTATCCA
                  ************************************************************

HsCD00513559      GGTGACAACACAAAACCCAAGACCACAGAGCCTACCTTTTCTCACCTGGCCTTGCCTGAG
NM_213598.3       GGTGACAACACAAAACCCAAGACCACAGAGCCTACCTTTTCTCACCTGGCCTTGCCTGAG
                  ************************************************************

HsCD00513559      GAAGTCTTACTCCAGGAACAACTGACACAAGGAGCCTCAAAGAACTCCCAATTAGGGCAA
NM_213598.3       GAAGTCTTACTCCAGGAACAACTGACACAAGGAGCCTCAAAGAACTCCCAATTAGGGCAA
                  ************************************************************

HsCD00513559      TCCAAGGATCAGGATGGGCCATCTGAAATGCAAGAAGTCCACTTGAAAATAGGGATAGGC
NM_213598.3       TCCAAGGATCAGGATGGGCCATCTGAAATGCAAGAAGTCCACTTGAAAATAGGGATAGGC
                  ************************************************************

HsCD00513559      CCCCAGCGGGGGAAGCTGCTGGAGAAAATGAGTTCTGAACGTGATGGTTTGGGGTCAGAT
NM_213598.3       CCCCAGCGGGGGAAGCTGCTGGAGAAAATGAGTTCTGAACGTGATGGTTTGGGGTCAGAT
                  ************************************************************

HsCD00513559      GATGGTGTATGTACAAAGATTACACAGAAACAAGTTTCAACAGAAGGTGATCTCTATGAA
NM_213598.3       GATGGTGTATGTACAAAGATTACACAGAAACAAGTTTCAACAGAAGGTGATCTCTATGAA
                  ************************************************************

HsCD00513559      TGTGATTCACATGGACCAGTTACAGATGCCTTGATTCGCGAAGAGAAAAATTCCTATAAA
NM_213598.3       TGTGATTCACATGGACCAGTTACAGATGCCTTGATTCGCGAAGAGAAAAATTCCTATAAA
                  ************************************************************

HsCD00513559      TGTGAGGAATGCGGGAAAGTGTTTAAAAAGAATGCCCTCCTTGTTCAGCATGAACGGATT
NM_213598.3       TGTGAGGAATGCGGGAAAGTGTTTAAAAAGAATGCCCTCCTTGTTCAGCATGAACGGATT
                  ************************************************************

HsCD00513559      CACACTCAAGTGAAGCCCTATGAATGCACAGAGTGTGGGAAAACCTTTAGCAAGAGCACT
NM_213598.3       CACACTCAAGTGAAGCCCTATGAATGCACAGAGTGTGGGAAAACCTTTAGCAAGAGCACT
                  ************************************************************

HsCD00513559      CATCTTCTTCAGCACCTCATCATCCACACTGGGGAGAAGCCCTATAAGTGCATGGAGTGT
NM_213598.3       CATCTTCTTCAGCACCTCATCATCCACACTGGGGAGAAGCCCTATAAGTGCATGGAGTGT
                  ************************************************************

HsCD00513559      GGGAAGGCTTTTAACCGCAGGTCACACCTCACACGGCACCAGCGGATTCACAGTGGAGAG
NM_213598.3       GGGAAGGCTTTTAACCGCAGGTCACACCTCACACGGCACCAGCGGATTCACAGTGGAGAG
                  ************************************************************

HsCD00513559      AAGCCTTATAAGTGCAGTGAATGTGGAAAGGCCTTCACCCACCGCTCCACTTTTGTCTTG
NM_213598.3       AAGCCTTATAAGTGCAGTGAATGTGGAAAGGCCTTCACCCACCGCTCCACTTTTGTCTTG
                  ************************************************************

HsCD00513559      CATCACAGGAGCCACACTGGAGAAAAACCCTTTGTGTGCAAAGAGTGTGGCAAAGCCTTT
NM_213598.3       CATCACAGGAGCCACACTGGAGAAAAACCCTTTGTGTGCAAAGAGTGTGGCAAAGCCTTT
                  ************************************************************

HsCD00513559      CGAGATAGGCCAGGTTTCATTCGACACTACATCATCCACACGGGAGAGAAGCCCTATGAG
NM_213598.3       CGAGATAGGCCAGGTTTCATTCGACACTACATCATCCACACGGGAGAGAAGCCCTATGAG
                  ************************************************************

HsCD00513559      TGCATTGAGTGTGGGAAGGCCTTCAACCGCCGGTCATACCTCACGTGGCACCAACAGATT
NM_213598.3       TGCATTGAGTGTGGGAAGGCCTTCAACCGCCGGTCATACCTCACGTGGCACCAACAGATT
                  ************************************************************

HsCD00513559      CACACTGGAGTGAAACCCTTTGAATGCAACGAGTGTGGAAAAGCTTTTTGCGAGAGTGCA
NM_213598.3       CACACTGGAGTGAAACCCTTTGAATGCAACGAGTGTGGAAAAGCTTTTTGCGAGAGTGCA
                  ************************************************************

HsCD00513559      GACCTCATTCAACACTACATTATCCACACTGGGGAGAAGCCCTATAAGTGCATGGAGTGT
NM_213598.3       GACCTCATTCAACACTACATTATCCACACTGGGGAGAAGCCCTATAAGTGCATGGAGTGT
                  ************************************************************

HsCD00513559      GGGAAGGCGTTCAACCGTAGGTCACACCTCAAGCAGCATCAACGGATTCACACTGGGGAG
NM_213598.3       GGGAAGGCGTTCAACCGTAGGTCACACCTCAAGCAGCATCAACGGATTCACACTGGGGAG
                  ************************************************************

HsCD00513559      AAGCCTTATGAATGCAGTGAATGTGGAAAGGCCTTCACCCACTGCTCCACTTTTGTCTTG
NM_213598.3       AAGCCTTATGAATGCAGTGAATGTGGAAAGGCCTTCACCCACTGCTCCACTTTTGTCTTG
                  ************************************************************

HsCD00513559      CATAAAAGGACCCACACAGGAGAAAAACCCTATGAATGCAAAGAATGTGGAAAAGCCTTT
NM_213598.3       CATAAAAGGACCCACACAGGAGAAAAACCCTATGAATGCAAAGAATGTGGAAAAGCCTTT
                  ************************************************************

HsCD00513559      AGTGATAGGGCAGACCTCATTCGCCACTTCAGCATCCACACTGGAGAGAAACCCTATGAG
NM_213598.3       AGTGATAGGGCAGACCTCATTCGCCACTTCAGCATCCACACTGGAGAGAAACCCTATGAG
                  ************************************************************

HsCD00513559      TGCGTGGAGTGTGGAAAGGCCTTCAACCGCAGCTCACACCTCACGAGGCACCAACAGATT
NM_213598.3       TGCGTGGAGTGTGGAAAGGCCTTCAACCGCAGCTCACACCTCACGAGGCACCAACAGATT
                  ************************************************************

HsCD00513559      CACACTGGAGAGAAACCCTATGAATGCATCCAGTGTGGGAAAGCCTTTTGCCGGAGCGCA
NM_213598.3       CACACTGGAGAGAAACCCTATGAATGCATCCAGTGTGGGAAAGCCTTTTGCCGGAGCGCA
                  ************************************************************

HsCD00513559      AACCTTATTCGACACTCCATCATTCACACTGGAGAGAAGCCGTATGAATGCAGTGAGTGT
NM_213598.3       AACCTTATTCGACACTCCATCATTCACACTGGAGAGAAGCCGTATGAATGCAGTGAGTGT
                  ************************************************************

HsCD00513559      GGAAAGGCTTTTAATCGCGGCTCATCCCTCACACATCATCAAAGGATTCATACTGGGAGA
NM_213598.3       GGAAAGGCTTTTAATCGCGGCTCATCCCTCACACATCATCAAAGGATTCATACTGGGAGA
                  ************************************************************

HsCD00513559      AACCCTACCATTGTAACAGATGTGGGAAGACCTTTTATGACTGCACAGACTTCAGTCAAC
NM_213598.3       AACCCTACCATTGTAACAGATGTGGGAAGACCTTTTATGACTGCACAGACTTCAGTCAAC
                  ************************************************************

HsCD00513559      ATCCAGGAACTTTTATTAGGGAAAGAGTTTTTGAATATCACCACTGAAGAAAATCTGTGG
NM_213598.3       ATCCAGGAACTTTTATTAGGGAAAGAGTTTTTGAATATCACCACTGAAGAAAATCTGTGG
                  ************************************************************

HsCD00513559      ---
NM_213598.3       TGA