DNASU Plasmid | ZNF296 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF296

Gene Name: zinc finger protein 296

Original Clone ID:

ccsbBroadEn_05121

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC019352.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513813

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1426nts

Open reading frame: 1 to 1426

Reference Sequence Alignment

ATGTCCCGCCGCAAGGCCGGCAGCGCGCCCCGCCGAGTAGAGCCCGCGCCCGCCGCCAAC
CCAGACGACGAGATGGAAATGCAGGACCTCGTCATCGAACTCAAGCCCGAGCCAGACGCG
CAGCCCCAACAGGCCCCAAGGCTGGGGCCCTTCTCCCCGAAGGAGGTGTCCTCGGCGGGG
CGGTTCGGCGGCGAACCCCACCACTCCCCTGGCCCCATGCCCGCCGGGGCCGCCCTCCTC
GCCCTCGGCCCGCGGAACCCGTGGACCCTGTGGACGCCGTTGACCCCGAACTATCCCGAC
CGCCAGCCCTGGACCGACAAACACCCAGATCTGTTGACCTGCGGCCGCTGCCTGCAGACC
TTCCCGTTGGAGGCCATCACTGCCTTCATGGACCACAAGAAGCTGGGCTGTCAGCTCTTC
AGAGGCCCCAGCCGCGGCCAGGGCTCAGAACGAGAGGAGCTGAAGGCCTTGAGCTGCCTG
CGCTGTGGCAAACAGTTCACAGTGGCCTGGAAGCTGCTGCGTCACGCCCAGTGGGACCAC
GGACTGTCCATCTACCAGACAGAATCAGAGGCCCCGGAGGCCCCGCTCCTGGGCCTGGCC
GAGGTGGCTGCAGCCGTGTCGGCAGTGGTGGGGCCAGCAGCTGAGGCCAAGAGCCCCCGT
GCAAGTGGCAGCGGCCTCACCCGGCGGAGCCCCACCTGTCCTGTGTGCAAGAAGACCCTC
AGCTCCTTCAGCAACCTCAAAGTGCACATGCGCTCACACACAGGCGAGCGGCCCTATGCT
TGCGACCAGTGTCCCTACGCCTGCGCCCAGAGCAGCAAGCTCAACCGCCACAAGAAGACC
CACCGGCAGGTGCCGCCCCAGAGCCCCCTCATGGCCGACACCAGCCAGGAGCAGGCCTCT
GCAGCCCCTCCGGAGCCGGCTGTCCATGCTGCTGCCCCCACCAGCACCCTTCCATGCAGC
GGTGGTGAGGGGGCTGGAGCCGCCGCCACAGCAGGTGTCCAGGAACCCGGGGCTCCTGGC
AGTGGGGCTCAAGCCGGCCCTGGTGGAGACACTTGGGGAGCCATCACCACGGAACAAAGA
ACTGACCCTGCAAACAGCCAGAAGGCATCACCCAAAAAGATGCCCAAGTCAGGGGGCAAG
AGCCGCGGGCCCGGGGGCAGCTGTGAGTTCTGCGGGAAGCATTTTACCAACAGCAGCAAC
CTGACGGTGCACCGGCGCTCACACACCGGGGAGCGCCCCTACACCTGTGAGTTCTGCAAC
TACGCCTGCGCCCAGAGCAGTAAGCTCAACCGCCACCGCCGCATGCACGGCATGACGCCT
GGCAGCACCCGCTTCGAGTGCCCCCACTGCCATGTGCCCTTCGGCCTGCGAGCCACCCTG
GACAAACACCTGCGGCAGAAGCACCCTGAGGCGGCCGGCGAGGCC

Protein sequence predicted based on nucleotide sequence

MSRRKAGSAPRRVEPAPAANPDDEMEMQDLVIELKPEPDAQPQQAPRLGPFSPKEVSSAG
RFGGEPHHSPGPMPAGAALLALGPRNPWTLWTPLTPNYPDRQPWTDKHPDLLTCGRCLQT
FPLEAITAFMDHKKLGCQLFRGPSRGQGSEREELKALSCLRCGKQFTVAWKLLRHAQWDH
GLSIYQTESEAPEAPLLGLAEVAAAVSAVVGPAAEAKSPRASGSGLTRRSPTCPVCKKTL
SSFSNLKVHMRSHTGERPYACDQCPYACAQSSKLNRHKKTHRQVPPQSPLMADTSQEQAS
AAPPEPAVHAAAPTSTLPCSGGEGAGAAATAGVQEPGAPGSGAQAGPGGDTWGAITTEQR
TDPANSQKASPKKMPKSGGKSRGPGGSCEFCGKHFTNSSNLTVHRRSHTGERPYTCEFCN
YACAQSSKLNRHRRMHGMTPGSTRFECPHCHVPFGLRATLDKHLRQKHPEAAGEA

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF296

Symbol Nomenclature :

ZNF296

SYNONYM :

ZFP296; ZNF342

Designation :

zinc finger protein 342

Full Nomenclature :

zinc finger protein 296

GENEID :

162979

HGNC :

15981

MIM :

613226

Vega :

OTTHUMG00000181766

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00513813      ATGTCCCGCCGCAAGGCCGGCAGCGCGCCCCGCCGAGTAGAGCCCGCGCCCGCCGCCAAC
NM_145288.1       ATGTCCCGCCGCAAGGCCGGCAGCGCGCCCCGCCGAGTAGAGCCCGCGCCCGCCGCCAAC
                  ************************************************************

HsCD00513813      CCAGACGACGAGATGGAAATGCAGGACCTCGTCATCGAACTCAAGCCCGAGCCAGACGCG
NM_145288.1       CCAGACGACGAGATGGAAATGCAGGACCTCGTCATCGAACTCAAGCCCGAGCCAGACGCG
                  ************************************************************

HsCD00513813      CAGCCCCAACAGGCCCCAAGGCTGGGGCCCTTCTCCCCGAAGGAGGTGTCCTCGGCGGGG
NM_145288.1       CAGCCCCAACAGGCCCCAAGGCTGGGGCCCTTCTCCCCGAAGGAGGTGTCCTCGGCGGGG
                  ************************************************************

HsCD00513813      CGGTTCGGCGGCGAACCCCACCACTCCCCTGGCCCCATGCCCGCCGGGGCCGCCCTCCTC
NM_145288.1       CGGTTCGGCGGCGAACCCCACCACTCCCCTGGCCCCATGCCCGCCGGGGCCGCCCTCCTC
                  ************************************************************

HsCD00513813      GCCCTCGGCCCGCGGAACCCGTGGACCCTGTGGACGCCGTTGACCCCGAACTATCCCGAC
NM_145288.1       GCCCTCGGCCCGCGGAACCCGTGGACCCTGTGGACGCCGTTGACCCCGAACTATCCCGAC
                  ************************************************************

HsCD00513813      CGCCAGCCCTGGACCGACAAACACCCAGATCTGTTGACCTGCGGCCGCTGCCTGCAGACC
NM_145288.1       CGCCAGCCCTGGACCGACAAACACCCAGATCTGTTGACCTGCGGCCGCTGCCTGCAGACC
                  ************************************************************

HsCD00513813      TTCCCGTTGGAGGCCATCACTGCCTTCATGGACCACAAGAAGCTGGGCTGTCAGCTCTTC
NM_145288.1       TTCCCGTTGGAGGCCATCACTGCCTTCATGGACCACAAGAAGCTGGGCTGTCAGCTCTTC
                  ************************************************************

HsCD00513813      AGAGGCCCCAGCCGCGGCCAGGGCTCAGAACGAGAGGAGCTGAAGGCCTTGAGCTGCCTG
NM_145288.1       AGAGGCCCCAGCCGCGGCCAGGGCTCAGAACGAGAGGAGCTGAAGGCCTTGAGCTGCCTG
                  ************************************************************

HsCD00513813      CGCTGTGGCAAACAGTTCACAGTGGCCTGGAAGCTGCTGCGTCACGCCCAGTGGGACCAC
NM_145288.1       CGCTGTGGCAAACAGTTCACAGTGGCCTGGAAGCTGCTGCGTCACGCCCAGTGGGACCAC
                  ************************************************************

HsCD00513813      GGACTGTCCATCTACCAGACAGAATCAGAGGCCCCGGAGGCCCCGCTCCTGGGCCTGGCC
NM_145288.1       GGACTGTCCATCTACCAGACAGAATCAGAGGCCCCGGAGGCCCCGCTCCTGGGCCTGGCC
                  ************************************************************

HsCD00513813      GAGGTGGCTGCAGCCGTGTCGGCAGTGGTGGGGCCAGCAGCTGAGGCCAAGAGCCCCCGT
NM_145288.1       GAGGTGGCTGCAGCCGTGTCGGCAGTGGTGGGGCCAGCAGCTGAGGCCAAGAGCCCCCGT
                  ************************************************************

HsCD00513813      GCAAGTGGCAGCGGCCTCACCCGGCGGAGCCCCACCTGTCCTGTGTGCAAGAAGACCCTC
NM_145288.1       GCAAGTGGCAGCGGCCTCACCCGGCGGAGCCCCACCTGTCCTGTGTGCAAGAAGACCCTC
                  ************************************************************

HsCD00513813      AGCTCCTTCAGCAACCTCAAAGTGCACATGCGCTCACACACAGGCGAGCGGCCCTATGCT
NM_145288.1       AGCTCCTTCAGCAACCTCAAAGTGCACATGCGCTCACACACAGGCGAGCGGCCCTATGCT
                  ************************************************************

HsCD00513813      TGCGACCAGTGTCCCTACGCCTGCGCCCAGAGCAGCAAGCTCAACCGCCACAAGAAGACC
NM_145288.1       TGCGACCAGTGTCCCTACGCCTGCGCCCAGAGCAGCAAGCTCAACCGCCACAAGAAGACC
                  ************************************************************

HsCD00513813      CACCGGCAGGTGCCGCCCCAGAGCCCCCTCATGGCCGACACCAGCCAGGAGCAGGCCTCT
NM_145288.1       CACCGGCAGGTGCCGCCCCAGAGCCCCCTCATGGCCGACACCAGCCAGGAGCAGGCCTCT
                  ************************************************************

HsCD00513813      GCAGCCCCTCCGGAGCCGGCTGTCCATGCTGCTGCCCCCACCAGCACCCTTCCATGCAGC
NM_145288.1       GCAGCCCCTCCGGAGCCGGCTGTCCATGCTGCTGCCCCCACCAGCACCCTTCCATGCAGC
                  ************************************************************

HsCD00513813      GGTGGTGAGGGGGCTGGAGCCGCCGCCACAGCAGGTGTCCAGGAACCCGGGGCTCCTGGC
NM_145288.1       GGTGGTGAGGGGGCTGGAGCCGCCGCCACAGCAGGTGTCCAGGAACCCGGGGCTCCTGGC
                  ************************************************************

HsCD00513813      AGTGGGGCTCAAGCCGGCCCTGGTGGAGACACTTGGGGAGCCATCACCACGGAACAAAGA
NM_145288.1       AGTGGGGCTCAAGCCGGCCCTGGTGGAGACACTTGGGGAGCCATCACCACGGAACAAAGA
                  ************************************************************

HsCD00513813      ACTGACCCTGCAAACAGCCAGAAGGCATCACCCAAAAAGATGCCCAAGTCAGGGGGCAAG
NM_145288.1       ACTGACCCTGCAAACAGCCAGAAGGCATCACCCAAAAAGATGCCCAAGTCAGGGGGCAAG
                  ************************************************************

HsCD00513813      AGCCGCGGGCCCGGGGGCAGCTGTGAGTTCTGCGGGAAGCATTTTACCAACAGCAGCAAC
NM_145288.1       AGCCGCGGGCCCGGGGGCAGCTGTGAGTTCTGCGGGAAGCATTTTACCAACAGCAGCAAC
                  ************************************************************

HsCD00513813      CTGACGGTGCACCGGCGCTCACACACCGGGGAGCGCCCCTACACCTGTGAGTTCTGCAAC
NM_145288.1       CTGACGGTGCACCGGCGCTCACACACCGGGGAGCGCCCCTACACCTGTGAGTTCTGCAAC
                  ************************************************************

HsCD00513813      TACGCCTGCGCCCAGAGCAGTAAGCTCAACCGCCACCGCCGCATGCACGGCATGACGCCT
NM_145288.1       TACGCCTGCGCCCAGAGCAGTAAGCTCAACCGCCACCGCCGCATGCACGGCATGACGCCT
                  ************************************************************

HsCD00513813      GGCAGCACCCGCTTCGAGTGCCCCCACTGCCATGTGCCCTTCGGCCTGCGAGCCACCCTG
NM_145288.1       GGCAGCACCCGCTTCGAGTGCCCCCACTGCCATGTGCCCTTCGGCCTGCGAGCCACCCTG
                  ************************************************************

HsCD00513813      GACAAACACCTGCGGCAGAAGCACCCTGAGGCGGCCGGCGAGGCC---
NM_145288.1       GACAAACACCTGCGGCAGAAGCACCCTGAGGCGGCCGGCGAGGCCTGA
                  *********************************************