DNASU Plasmid | ZNF554 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF554

Gene Name: zinc finger protein 554

Original Clone ID:

ccsbBroadEn_09413

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC047777.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513869

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1615nts

Open reading frame: 1 to 1615

Reference Sequence Alignment

ATGGTCACCTGCGCCCACCTGGGCCGGCGCGCGCGACTCCCGGCAGCTCAGCCCTCTGCC
TGCCCAGGAACCTGCTTTTCCCAAGAGGAGAGAATGGCTGCTGGGTACCTGCCCCGCTGG
TCCCAGGAATTAGTAACCTTTGAGGACGTGTCCATGGACTTCTCCCAGGAGGAGTGGGAG
TTGCTGGAGCCTGCTCAGAAGAACCTGTACAGAGAGGTGATGCTGGAGAACTACAGGAAC
GTGGTCTCCCTGGAAGCCTTGAAGAACCAATGTACTGATGTGGGGATTAAAGAGGGTCCA
CTTTCCCCAGCACAAACCTCACAAGTCACTAGTCTTTCCTCATGGACGGGGTATTTACTT
TTTCAACCAGTGGCTTCTTCCCACTTGGAGCAAAGAGAAGCCCTGTGGATAGAGGAAAAA
GGAACTCCTCAAGCCTCCTGTTCAGATTGGATGACTGTACTAAGAAACCAAGACTCAACT
TACAAGAAGGTGGCTTTGCAGGAGGAACCAGCCAGTGGTATAAATATGATAAAGCTTATC
AGAGAAGATGGGGGATGGAAGCAGTTAGAGGACAGCCATGAAGACCCCCAGGGGCTTTTG
AGCCAAAAGGCATCCCTTCACGTAGTGGCCGTTCCTCAGGAGAAGGCTACTGCATGGCAT
GGATTTGGGGAAAATGGTAATCTGAGCCCAGCCCTTGTTTTATCACAGGGAAGCTCTAAA
GGGAACCACTTGTGTGGCAGCGAGTTAGATATTACAAGCTTGGCATCCGATTCAGTCTTA
AACCACCATCAGCTGGGATATGCAGATCGGAGACCTTGTGAAAGTAATGAATGTGGAAAT
GCCATCCGCCAGAACAGTCACTTTATTCAACACGGGGGGAAGATGTTTGTGTATTTGGAA
AATGGGCAGTCATTGAACCACGGTATGGCCCTGACTATCCACAACAAAATCAACACGGCA
GAGAAACCCTTTGAGTGCCACCAGTGTGGGAAGGTGTTCAACCGGAGGCATTCTTTGAGC
GAACATCAAAGAATTCACACGGGGGAGAAACCCTACGAGTGTCAGGAGTGTGGGCGAGCC
TTTACGCACAGCTCCACCCTCACGCGCCATCTGAGAACTCATACTGGAGAGAAGCCCTAC
GGGTGCGGTGAGTGCGGGAAAGCCTTCAACAGGATCTCATCGCTGACTCAGCATCAGAGG
ATTCACACCGGGGAAAAGCCCTATAAATGTGAAGACTGTGGGAAATCCTTCTGCCAGAGC
TCTTACCTGATCTTGCACAAGAGGACACACACCGGAGAGAAGCCCTACGAATGCAGTGAA
TGTGGAAAGGCCTTCAGTGACCGTTCCTCTCTCAACCAGCACGAGCGAACTCACACGGGC
GAGAACCCCTATGAATGTAAGCAGTGTGGGAGAGCCTTCAGCCAGAGGTCTTCCCTTGTG
AGGCACGAGAGAACTCACACTGGAGAGAAACCCTACAGGTGTCAGGAATGTGGGAAAGCC
TTCAGCCAGAGCTCATCCCTTGTCACACATCAGAAAACTCACAGCAGCCAGAAAACCTAT
AAAATCATTGACTGCGGGAAAGCGTTCTACCAGAACAGACATCTTATTGGATAT

Protein sequence predicted based on nucleotide sequence

MVTCAHLGRRARLPAAQPSACPGTCFSQEERMAAGYLPRWSQELVTFEDVSMDFSQEEWE
LLEPAQKNLYREVMLENYRNVVSLEALKNQCTDVGIKEGPLSPAQTSQVTSLSSWTGYLL
FQPVASSHLEQREALWIEEKGTPQASCSDWMTVLRNQDSTYKKVALQEEPASGINMIKLI
REDGGWKQLEDSHEDPQGLLSQKASLHVVAVPQEKATAWHGFGENGNLSPALVLSQGSSK
GNHLCGSELDITSLASDSVLNHHQLGYADRRPCESNECGNAIRQNSHFIQHGGKMFVYLE
NGQSLNHGMALTIHNKINTAEKPFECHQCGKVFNRRHSLSEHQRIHTGEKPYECQECGRA
FTHSSTLTRHLRTHTGEKPYGCGECGKAFNRISSLTQHQRIHTGEKPYKCEDCGKSFCQS
SYLILHKRTHTGEKPYECSECGKAFSDRSSLNQHERTHTGENPYECKQCGRAFSQRSSLV
RHERTHTGEKPYRCQECGKAFSQSSSLVTHQKTHSSQKTYKIIDCGKAFYQNRHLIGY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF554

Symbol Nomenclature :

ZNF554

Full Nomenclature :

zinc finger protein 554

GENEID :

115196

HGNC :

26629

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00513869        ATGGTCACCTGCGCCCACCTGGGCCGGCGCGCGCGACTCCCGGCAGCTCAGCCCTCTGCC
NM_001102651.1      ATGGTCACCTGCGCCCACCTGGGCCGGCGCGCGCGGCTCCCGGCAGCTCAGCCCTCTGCC
                    ***********************************.************************

HsCD00513869        TGCCCAGGAACCTGCTTTTCCCAAGAGGAGAGAATGGCTGCTGGGTACCTGCCCCGCTGG
NM_001102651.1      TGCCCAGGAACCTGCTTTTCCCAAGAGGAGAGAATGGCTGCTGGGTACCTGCCCCGCTGG
                    ************************************************************

HsCD00513869        TCCCAGGAATTAGTAACCTTTGAGGACGTGTCCATGGACTTCTCCCAGGAGGAGTGGGAG
NM_001102651.1      TCCCAGGAATTAGTAACCTTTGAGGACGTGTCCATGGACTTCTCCCAGGAGGAGTGGGAG
                    ************************************************************

HsCD00513869        TTGCTGGAGCCTGCTCAGAAGAACCTGTACAGAGAGGTGATGCTGGAGAACTACAGGAAC
NM_001102651.1      TTGCTGGAGCCTGCTCAGAAGAACCTGTACAGAGAGGTGATGCTGGAGAACTACAGGAAC
                    ************************************************************

HsCD00513869        GTGGTCTCCCTGGAAGCCTTGAAGAACCAATGTACTGATGTGGGGATTAAAGAGGGTCCA
NM_001102651.1      GTGGTCTCCCTGGAAGCCTTGAAGAACCAATGTACTGATGTGGGGATTAAAGAGGGTCCA
                    ************************************************************

HsCD00513869        CTTTCCCCAGCACAAACCTCACAAGTCACTAGTCTTTCCTCATGGACGGGGTATTTACTT
NM_001102651.1      CTTTCCCCAGCACAAACCTCACAAGTCACTAGTCTTTCCTCATGGACGGGGTATTTACTT
                    ************************************************************

HsCD00513869        TTTCAACCAGTGGCTTCTTCCCACTTGGAGCAAAGAGAAGCCCTGTGGATAGAGGAAAAA
NM_001102651.1      TTTCAACCAGTGGCTTCTTCCCACTTGGAGCAAAGAGAAGCCCTGTGGATAGAGGAAAAA
                    ************************************************************

HsCD00513869        GGAACTCCTCAAGCCTCCTGTTCAGATTGGATGACTGTACTAAGAAACCAAGACTCAACT
NM_001102651.1      GGAACTCCTCAAGCCTCCTGTTCAGATTGGATGACTGTACTAAGAAACCAAGACTCAACT
                    ************************************************************

HsCD00513869        TACAAGAAGGTGGCTTTGCAGGAGGAACCAGCCAGTGGTATAAATATGATAAAGCTTATC
NM_001102651.1      TACAAGAAGGTGGCTTTGCAGGAGGAACCAGCCAGTGGTATAAATATGATAAAGCTTATC
                    ************************************************************

HsCD00513869        AGAGAAGATGGGGGATGGAAGCAGTTAGAGGACAGCCATGAAGACCCCCAGGGGCTTTTG
NM_001102651.1      AGAGAAGATGGGGGATGGAAGCAGTTAGAGGACAGCCATGAAGACCCCCAGGGGCTTTTG
                    ************************************************************

HsCD00513869        AGCCAAAAGGCATCCCTTCACGTAGTGGCCGTTCCTCAGGAGAAGGCTACTGCATGGCAT
NM_001102651.1      AGCCAAAAGGCATCCCTTCACGTAGTGGCCGTTCCTCAGGAGAAGGCTACTGCATGGCAT
                    ************************************************************

HsCD00513869        GGATTTGGGGAAAATGGTAATCTGAGCCCAGCCCTTGTTTTATCACAGGGAAGCTCTAAA
NM_001102651.1      GGATTTGGGGAAAATGGTAATCTGAGCCCAGCCCTTGTTTTATCACAGGGAAGCTCTAAA
                    ************************************************************

HsCD00513869        GGGAACCACTTGTGTGGCAGCGAGTTAGATATTACAAGCTTGGCATCCGATTCAGTCTTA
NM_001102651.1      GGGAACCACTTGTGTGGCAGCGAGTTAGATATTACAAGCTTGGCATCCGATTCAGTCTTA
                    ************************************************************

HsCD00513869        AACCACCATCAGCTGGGATATGCAGATCGGAGACCTTGTGAAAGTAATGAATGTGGAAAT
NM_001102651.1      AACCACCATCAGCTGGGATATGCAGATCGGAGACCTTGTGAAAGTAATGAATGTGGAAAT
                    ************************************************************

HsCD00513869        GCCATCCGCCAGAACAGTCACTTTATTCAACACGGGGGGAAGATGTTTGTGTATTTGGAA
NM_001102651.1      GCCATCCGCCAGAACAGTCACTTTATTCAACACGGGGGGAAGATGTTTGTGTATTTGGAA
                    ************************************************************

HsCD00513869        AATGGGCAGTCATTGAACCACGGTATGGCCCTGACTATCCACAACAAAATCAACACGGCA
NM_001102651.1      AATGGGCAGTCATTGAACCACGGTATGGCCCTGACTATCCACAACAAAATCAACACGGCA
                    ************************************************************

HsCD00513869        GAGAAACCCTTTGAGTGCCACCAGTGTGGGAAGGTGTTCAACCGGAGGCATTCTTTGAGC
NM_001102651.1      GAGAAACCCTTTGAGTGCCACCAGTGTGGGAAGGTGTTCAACCGGAGGCATTCTTTGAGC
                    ************************************************************

HsCD00513869        GAACATCAAAGAATTCACACGGGGGAGAAACCCTACGAGTGTCAGGAGTGTGGGCGAGCC
NM_001102651.1      GAACATCAAAGAATTCACACGGGGGAGAAACCCTACGAGTGTCAGGAGTGTGGGCGAGCC
                    ************************************************************

HsCD00513869        TTTACGCACAGCTCCACCCTCACGCGCCATCTGAGAACTCATACTGGAGAGAAGCCCTAC
NM_001102651.1      TTTACGCACAGCTCCACCCTCACGCGCCATCTGAGAACTCATACTGGAGAGAAGCCCTAC
                    ************************************************************

HsCD00513869        GGGTGCGGTGAGTGCGGGAAAGCCTTCAACAGGATCTCATCGCTGACTCAGCATCAGAGG
NM_001102651.1      GGGTGCGGTGAGTGCGGGAAAGCCTTCAACAGGATCTCATCGCTGACTCAGCATCAGAGG
                    ************************************************************

HsCD00513869        ATTCACACCGGGGAAAAGCCCTATAAATGTGAAGACTGTGGGAAATCCTTCTGCCAGAGC
NM_001102651.1      ATTCACACCGGGGAAAAGCCCTATAAATGTGAAGACTGTGGGAAATCCTTCTGCCAGAGC
                    ************************************************************

HsCD00513869        TCTTACCTGATCTTGCACAAGAGGACACACACCGGAGAGAAGCCCTACGAATGCAGTGAA
NM_001102651.1      TCTTACCTGATCTTGCACAAGAGGACACACACCGGAGAGAAGCCCTACGAATGCAGTGAA
                    ************************************************************

HsCD00513869        TGTGGAAAGGCCTTCAGTGACCGTTCCTCTCTCAACCAGCACGAGCGAACTCACACGGGC
NM_001102651.1      TGTGGAAAGGCCTTCAGTGACCGTTCCTCTCTCAACCAGCACGAGCGAACTCACACGGGC
                    ************************************************************

HsCD00513869        GAGAACCCCTATGAATGTAAGCAGTGTGGGAGAGCCTTCAGCCAGAGGTCTTCCCTTGTG
NM_001102651.1      GAGAACCCCTATGAATGTAAGCAGTGTGGGAGAGCCTTCAGCCAGAGGTCTTCCCTTGTG
                    ************************************************************

HsCD00513869        AGGCACGAGAGAACTCACACTGGAGAGAAACCCTACAGGTGTCAGGAATGTGGGAAAGCC
NM_001102651.1      AGGCACGAGAGAACTCACACTGGAGAGAAACCCTACAGGTGTCAGGAATGTGGGAAAGCC
                    ************************************************************

HsCD00513869        TTCAGCCAGAGCTCATCCCTTGTCACACATCAGAAAACTCACAGCAGCCAGAAAACCTAT
NM_001102651.1      TTCAGCCAGAGCTCATCCCTTGTCACACATCAGAAAACTCACAGCAGCCAGAAAACCTAT
                    ************************************************************

HsCD00513869        AAAATCATTGACTGCGGGAAAGCGTTCTACCAGAACAGACATCTTATTGGATAT---
NM_001102651.1      AAAATCATTGACTGTGGGAAAGCGTTCTACCAGAACAGACATCTTATTGGATATTAG
                    ************** ***************************************