DNASU Plasmid | C12orf4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: C12orf4

Gene Name: chromosome 12 open reading frame 4

Original Clone ID:

ccsbBroadEn_03781

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC016908.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513918

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1657nts

Open reading frame: 1 to 1657

Reference Sequence Alignment

ATGAAGAAAAACAGAGAAAGATTCTGCAATAGAGAGAGAGAATTTGTATATAAATTTAAA
GTAGGAAGTCAGTGCTTAGAACTGAGAGTGCCACTCAAATTTCCTGTTCAAGAGAATGCC
AGTCATTTGCATGGACGTCTGATGCTGCTGCACAGTTTACCGTGCTTTATAGAAAAAGAC
TTAAAAGAAGCTCTGACTCAGTTTATAGAAGAAGAATCCCTCAGCGATTATGATAGAGAT
GCTGAAGCATCCCTGGCAGCTGTGAAATCAGGTGAAGTAGATTTACATCAGCTGGCGAGT
ACATGGGCCAAAGCTTATGCTGAGACCACGTTAGAGCATGCAAGGCCTGAAGAACCCAGC
TGGGATGAAGATTTTGCAGATGTGTACCATGACTTAATTCATTCTCCTGCCTCTGAAACT
CTCTTAAATTTGGAACATAATTACTTTGTTAGTATCTCAGAACTGATTGGTGAAAGAGAT
GTGGAGCTGAAAAAATTACGAGAGAGACAAGGTATTGAAATGGAAAAAGTCATGCAGGAA
TTGGGAAAATCACTGACAGATCAAGATGTAAATTCACTGGCTGCTCAGCATTTTGAATCC
CAGCAAGACCTAGAAAATAAATGGTCGAATGAATTAAAACAATCAACTGCCATCCAAAAA
CAAGAGTATCAAGAATGGGTAATAAAACTTCACCAAGACCTAAAAAACCCCAACAACAGC
TCCCTTAGTGAGGAAATTAAAGTTCAGCCAAGTCAGTTCAGAGAATCTGTAGAAGCAATT
GGAAGGATTTATGAGGAACAGAGAAAGTTAGAAGAAAGTTTTACCATTCACTTAGGAGCC
CAGTTGAAGACCATGCATAATTTGAGATTGCTGAGAGCAGATATGCTGGACTTCTGTAAG
CATAAAAGAAATCATCGAAGTGGTGTGAAACTTCATCGGCTCCAAACAGCTCTGTCACTT
TATTCTACATCTCTCTGTGGCCTGGTTTTACTAGTAGATAATCGAATTAATTCATATAGT
GGTATTAAAAGAGATTTTGCCACAGTTTGCCAAGAATGCACTGACTTCCATTTCCCCCGA
ATTGAAGAGCAATTAGAAGTTGTCCAACAGGTGGTACTTTATGCTAGAACCCAGCGCAGG
AGTAAATTGAAAGAATCACTTGATTCTGGAAACCAAAATGGAGGAAATGATGATAAGACT
AAGAATGCTGAGAGGAACTATTTAAATGTTTTACCTGGGGAATTTTATATTACACGGCAT
TCTAATCTCTCAGAAATCCATGTTGCTTTCCATCTCTGTGTGGATGACCATGTGAAATCG
GGAAACATCACTGCTCGTGATCCTGCCATTATGGGACTCCGAAATATACTCAAAGTTTGC
TGTACCCATGACATCACAACAATAAGCATTCCTCTCTTGCTGGTACATGATATGTCAGAG
GAAATGACTATACCCTGGTGCTTAAGGAGAGCGGAACTTGTGTTCAAGTGTGTCAAAGGT
TTCATGATGGAAATGGCTTCATGGGATGGAGGAATTTCTAGGACAGTGCAATTTCTAGTA
CCACAGAGTATTTCTGAAGAAATGTTTTATCAACTTAGTAACATGCTTCCCCAGATCTTC
CGAGTATCATCAACACTCACTCTGACATCCAAGCAC

Protein sequence predicted based on nucleotide sequence

MKKNRERFCNREREFVYKFKVGSQCLELRVPLKFPVQENASHLHGRLMLLHSLPCFIEKD
LKEALTQFIEEESLSDYDRDAEASLAAVKSGEVDLHQLASTWAKAYAETTLEHARPEEPS
WDEDFADVYHDLIHSPASETLLNLEHNYFVSISELIGERDVELKKLRERQGIEMEKVMQE
LGKSLTDQDVNSLAAQHFESQQDLENKWSNELKQSTAIQKQEYQEWVIKLHQDLKNPNNS
SLSEEIKVQPSQFRESVEAIGRIYEEQRKLEESFTIHLGAQLKTMHNLRLLRADMLDFCK
HKRNHRSGVKLHRLQTALSLYSTSLCGLVLLVDNRINSYSGIKRDFATVCQECTDFHFPR
IEEQLEVVQQVVLYARTQRRSKLKESLDSGNQNGGNDDKTKNAERNYLNVLPGEFYITRH
SNLSEIHVAFHLCVDDHVKSGNITARDPAIMGLRNILKVCCTHDITTISIPLLLVHDMSE
EMTIPWCLRRAELVFKCVKGFMMEMASWDGGISRTVQFLVPQSISEEMFYQLSNMLPQIF
RVSSTLTLTSKH

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

C12orf4

Symbol Nomenclature :

C12orf4

Designation :

uncharacterized protein C12orf4

Full Nomenclature :

chromosome 12 open reading frame 4

GENEID :

57102

GenBank Accession :

JF432291

HGNC :

1184

Vega :

OTTHUMG00000168258

Target GenBank:

JF432291

Reference Sequence Alignment

  

HsCD00513918        ATGAAGAAAAACAGAGAAAGATTCTGCAATAGAGAGAGAGAATTTGTATATAAATTTAAA
NM_020374.2         ATGAAGAAAAACAGAGAAAGATTCTGCAATAGAGAGAGAGAATTTGTATATAAATTTAAA
XM_005253716.1      ATGAAGAAAAACAGAGAAAGATTCTGCAATAGAGAGAGAGAATTTGTATATAAATTTAAA
                    ************************************************************

HsCD00513918        GTAGGAAGTCAGTGCTTAGAACTGAGAGTGCCACTCAAATTTCCTGTTCAAGAGAATGCC
NM_020374.2         GTAGGAAGTCAGTGCTTAGAACTGAGAGTGCCACTCAAATTTCCTGTTCAAGAGAATGCC
XM_005253716.1      GTAGGAAGTCAGTGCTTAGAACTGAGAGTGCCACTCAAATTTCCTGTTCAAGAGAATGCC
                    ************************************************************

HsCD00513918        AGTCATTTGCATGGACGTCTGATGCTGCTGCACAGTTTACCGTGCTTTATAGAAAAAGAC
NM_020374.2         AGTCATTTGCATGGACGTCTGATGCTGCTGCACAGTTTACCGTGCTTTATAGAAAAAGAC
XM_005253716.1      AGTCATTTGCATGGACGTCTGATGCTGCTGCACAGTTTACCGTGCTTTATAGAAAAAGAC
                    ************************************************************

HsCD00513918        TTAAAAGAAGCTCTGACTCAGTTTATAGAAGAAGAATCCCTCAGCGATTATGATAGAGAT
NM_020374.2         TTAAAAGAAGCTCTGACTCAGTTTATAGAAGAAGAATCCCTCAGCGATTATGATAGAGAT
XM_005253716.1      TTAAAAGAAGCTCTGACTCAGTTTATAGAAGAAGAATCCCTCAGCGATTATGATAGAGAT
                    ************************************************************

HsCD00513918        GCTGAAGCATCCCTGGCAGCTGTGAAATCAGGTGAAGTAGATTTACATCAGCTGGCGAGT
NM_020374.2         GCTGAAGCATCCCTGGCAGCTGTGAAATCAGGTGAAGTAGATTTACATCAGCTGGCGAGT
XM_005253716.1      GCTGAAGCATCCCTGGCAGCTGTGAAATCAGGTGAAGTAGATTTACATCAGCTGGCGAGT
                    ************************************************************

HsCD00513918        ACATGGGCCAAAGCTTATGCTGAGACCACGTTAGAGCATGCAAGGCCTGAAGAACCCAGC
NM_020374.2         ACATGGGCCAAAGCTTATGCTGAGACCACGTTAGAGCATGCAAGGCCTGAAGAACCCAGC
XM_005253716.1      ACATGGGCCAAAGCTTATGCTGAGACCACGTTAGAGCATGCAAGGCCTGAAGAACCCAGC
                    ************************************************************

HsCD00513918        TGGGATGAAGATTTTGCAGATGTGTACCATGACTTAATTCATTCTCCTGCCTCTGAAACT
NM_020374.2         TGGGATGAAGATTTTGCAGATGTGTACCATGACTTAATTCATTCTCCTGCCTCTGAAACT
XM_005253716.1      TGGGATGAAGATTTTGCAGATGTGTACCATGACTTAATTCATTCTCCTGCCTCTGAAACT
                    ************************************************************

HsCD00513918        CTCTTAAATTTGGAACATAATTACTTTGTTAGTATCTCAGAACTGATTGGTGAAAGAGAT
NM_020374.2         CTCTTAAATTTGGAACATAATTACTTTGTTAGTATCTCAGAACTGATTGGTGAAAGAGAT
XM_005253716.1      CTCTTAAATTTGGAACATAATTACTTTGTTAGTATCTCAGAACTGATTGGTGAAAGAGAT
                    ************************************************************

HsCD00513918        GTGGAGCTGAAAAAATTACGAGAGAGACAAGGTATTGAAATGGAAAAAGTCATGCAGGAA
NM_020374.2         GTGGAGCTGAAAAAATTACGAGAGAGACAAGGTATTGAAATGGAAAAAGTCATGCAGGAA
XM_005253716.1      GTGGAGCTGAAAAAATTACGAGAGAGACAAGGTATTGAAATGGAAAAAGTCATGCAGGAA
                    ************************************************************

HsCD00513918        TTGGGAAAATCACTGACAGATCAAGATGTAAATTCACTGGCTGCTCAGCATTTTGAATCC
NM_020374.2         TTGGGAAAATCACTGACAGATCAAGATGTAAATTCACTGGCTGCTCAGCATTTTGAATCC
XM_005253716.1      TTGGGAAAATCACTGACAGATCAAGATGTAAATTCACTGGCTGCTCAGCATTTTGAATCC
                    ************************************************************

HsCD00513918        CAGCAAGACCTAGAAAATAAATGGTCGAATGAATTAAAACAATCAACTGCCATCCAAAAA
NM_020374.2         CAGCAAGACCTAGAAAATAAATGGTCGAATGAATTAAAACAATCAACTGCCATCCAAAAA
XM_005253716.1      CAGCAAGACCTAGAAAATAAATGGTCGAATGAATTAAAACAATCAACTGCCATCCAAAAA
                    ************************************************************

HsCD00513918        CAAGAGTATCAAGAATGGGTAATAAAACTTCACCAAGACCTAAAAAACCCCAACAACAGC
NM_020374.2         CAAGAGTATCAAGAATGGGTAATAAAACTTCACCAAGACCTAAAAAACCCCAACAACAGC
XM_005253716.1      CAAGAGTATCAAGAATGGGTAATAAAACTTCACCAAGACCTAAAAAACCCCAACAACAGC
                    ************************************************************

HsCD00513918        TCCCTTAGTGAGGAAATTAAAGTTCAGCCAAGTCAGTTCAGAGAATCTGTAGAAGCAATT
NM_020374.2         TCCCTTAGTGAGGAAATTAAAGTTCAGCCAAGTCAGTTCAGAGAATCTGTAGAAGCAATT
XM_005253716.1      TCCCTTAGTGAGGAAATTAAAGTTCAGCCAAGTCAGTTCAGAGAATCTGTAGAAGCAATT
                    ************************************************************

HsCD00513918        GGAAGGATTTATGAGGAACAGAGAAAGTTAGAAGAAAGTTTTACCATTCACTTAGGAGCC
NM_020374.2         GGAAGGATTTATGAGGAACAGAGAAAGTTAGAAGAAAGTTTTACCATTCACTTAGGAGCC
XM_005253716.1      GGAAGGATTTATGAGGAACAGAGAAAGTTAGAAGAAAGTTTTACCATTCACTTAGGAGCC
                    ************************************************************

HsCD00513918        CAGTTGAAGACCATGCATAATTTGAGATTGCTGAGAGCAGATATGCTGGACTTCTGTAAG
NM_020374.2         CAGTTGAAGACCATGCATAATTTGAGATTGCTGAGAGCAGATATGCTGGACTTCTGTAAG
XM_005253716.1      CAGTTGAAGACCATGCATAATTTGAGATTGCTGAGAGCAGATATGCTGGACTTCTGTAAG
                    ************************************************************

HsCD00513918        CATAAAAGAAATCATCGAAGTGGTGTGAAACTTCATCGGCTCCAAACAGCTCTGTCACTT
NM_020374.2         CATAAAAGAAATCATCGAAGTGGTGTGAAACTTCATCGGCTCCAAACAGCTCTGTCACTT
XM_005253716.1      CATAAAAGAAATCATCGAAGTGGTGTGAAACTTCATCGGCTCCAAACAGCTCTGTCACTT
                    ************************************************************

HsCD00513918        TATTCTACATCTCTCTGTGGCCTGGTTTTACTAGTAGATAATCGAATTAATTCATATAGT
NM_020374.2         TATTCTACATCTCTCTGTGGCCTGGTTTTACTAGTAGATAATCGAATTAATTCATATAGT
XM_005253716.1      TATTCTACATCTCTCTGTGGCCTGGTTTTACTAGTAGATAATCGAATTAATTCATATAGT
                    ************************************************************

HsCD00513918        GGTATTAAAAGAGATTTTGCCACAGTTTGCCAAGAATGCACTGACTTCCATTTCCCCCGA
NM_020374.2         GGTATTAAAAGAGATTTTGCCACAGTTTGCCAAGAATGCACTGACTTCCATTTCCCCCGA
XM_005253716.1      GGTATTAAAAGAGATTCT------------------------------------------
                    **************** *                                          

HsCD00513918        ATTGAAGAGCAATTAGAAGTTGTCCAACAGGTGGTACTTTATGCTAGAACCCAGCGCAGG
NM_020374.2         ATTGAAGAGCAATTAGAAGTTGTCCAACAGGTGGTACTTTATGCTAGAACCCAGCGCAGG
XM_005253716.1      ------------------------------------------------------------
                                                                                

HsCD00513918        AGTAAATTGAAAGAATCACTTGATTCTGGAAACCAAAATGGAGGAAATGATGATAAGACT
NM_020374.2         AGTAAATTGAAAGAATCACTTGATTCTGGAAACCAAAATGGAGGAAATGATGATAAGACT
XM_005253716.1      ---------------------------GGAAACCAAAATGGAGGAAATGATGATAAGACT
                                               *********************************

HsCD00513918        AAGAATGCTGAGAGGAACTATTTAAATGTTTTACCTGGGGAATTTTATATTACACGGCAT
NM_020374.2         AAGAATGCTGAGAGGAACTATTTAAATGTTTTACCTGGGGAATTTTATATTACACGGCAT
XM_005253716.1      AAGAATGCTGAGAGGAACTATTTAAATGTTTTACCTGGGGAATTTTATATTACACGGCAT
                    ************************************************************

HsCD00513918        TCTAATCTCTCAGAAATCCATGTTGCTTTCCATCTCTGTGTGGATGACCATGTGAAATCG
NM_020374.2         TCTAATCTCTCAGAAATCCATGTTGCTTTCCATCTCTGTGTGGATGACCATGTGAAATCG
XM_005253716.1      TCTAATCTCTCAGAAATCCATGTTGCTTTCCATCTCTGTGTGGATGACCATGTGAAATCG
                    ************************************************************

HsCD00513918        GGAAACATCACTGCTCGTGATCCTGCCATTATGGGACTCCGAAATATACTCAAAGTTTGC
NM_020374.2         GGAAACATCACTGCTCGTGATCCTGCCATTATGGGACTCCGAAATATACTCAAAGTTTGC
XM_005253716.1      GGAAACATCACTGCTCGTGATCCTGCCATTATGGGACTCCGAAATATACTCAAAGTTTGC
                    ************************************************************

HsCD00513918        TGTACCCATGACATCACAACAATAAGCATTCCTCTCTTGCTGGTACATGATATGTCAGAG
NM_020374.2         TGTACCCATGACATCACAACAATAAGCATTCCTCTCTTGCTGGTACATGATATGTCAGAG
XM_005253716.1      TGTACCCATGACATCACAACAATAAGCATTCCTCTCTTGCTGGTACATGATATGTCAGAG
                    ************************************************************

HsCD00513918        GAAATGACTATACCCTGGTGCTTAAGGAGAGCGGAACTTGTGTTCAAGTGTGTCAAAGGT
NM_020374.2         GAAATGACTATACCCTGGTGCTTAAGGAGAGCGGAACTTGTGTTCAAGTGTGTCAAAGGT
XM_005253716.1      GAAATGACTATACCCTGGTGCTTAAGGAGAGCGGAACTTGTGTTCAAGTGTGTCAAAGGT
                    ************************************************************

HsCD00513918        TTCATGATGGAAATGGCTTCATGGGATGGAGGAATTTCTAGGACAGTGCAATTTCTAGTA
NM_020374.2         TTCATGATGGAAATGGCTTCATGGGATGGAGGAATTTCTAGGACAGTGCAATTTCTAGTA
XM_005253716.1      TTCATGATGGAAATGGCTTCATGGGATGGAGGAATTTCTAGGACAGTGCAATTTCTAGTA
                    ************************************************************

HsCD00513918        CCACAGAGTATTTCTGAAGAAATGTTTTATCAACTTAGTAACATGCTTCCCCAGATCTTC
NM_020374.2         CCACAGAGTATTTCTGAAGAAATGTTTTATCAACTTAGTAACATGCTTCCCCAGATCTTC
XM_005253716.1      CCACAGAGTATTTCTGAAGAAATGTTTTATCAACTTAGTAACATGCTTCCCCAGATCTTC
                    ************************************************************

HsCD00513918        CGAGTATCATCAACACTCACTCTGACATCCAAGCAC---
NM_020374.2         CGAGTATCATCAACACTCACTCTGACATCCAAGCACTAA
XM_005253716.1      CGAGTATCATCAACACTCACTCTGACATCCAAGCACTAA
                    ************************************