DNASU Plasmid | VPS16 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: VPS16

Gene Name: vacuolar protein sorting 16 homolog (S. cerevisiae)

Original Clone ID:

ccsbBroadEn_12476

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC073959.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513933

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1573nts

Open reading frame: 1 to 1573

Reference Sequence Alignment

ATGCCCTGGCTCTGGACTGCTCCCCACCAGAAAGAGAGCCAGAAGGCGGACGAGTACCTG
CGGGAGATCCAGGAGCTGGGCCAGCTGACCCAGGCCGTGCAGCAGTGCATTGAGGCTGCA
GGACATGAGCACCAGCCAGACATGCAGAAGAGTCTGCTCAGGGTTGGCCTGGATGGCCGG
GCCGGGGAGGGTGGGGCTGGGAGGGGGTGGGATGGGCAGCAGGGAAGCTCTCTCCTATCG
CCCTCTGGCTCTTCTCAGGCCGCCTCCTTCGGAAAGTGTTTCCTGGACAGATTTCCACCC
GACAGCTTCGTGCACATGTGTCAGGACCTGCGTGTGCTCAATGCTGTTCGGGACTATCAC
ATCGGGATCCCGCTCACCTATAGCCAATATAAGCAGCTCACCATCCAGGTGCTGCTGGAC
AGGCTCGTGTTGCGGAGACTTTACCCCCTGGCCATCCAGATATGCGAGTACTTGCGCCTT
CCTGAAGTACAGGGCGTCAGCAGGATCCTGGCCCACTGGGCCTGCTACAAGGTGCAACAG
AAGGATGTCTCAGATGAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGGGACACGCCT
GGTGTCTCTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGCTGGCC
ATCAAGCTGCTGGAGTATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATG
AAGAGGAGCAAACTGGCACTAAGCAAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTC
ACGGTGTTGCTGCACCTGAAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGG
AATCAGCCCATGGCCCTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGAGCTAGAGACG
CTGAAGGACCTTTACAATCAGGATGACAATCACCAGGAATTGGGCAGCTTCCACATCCGA
GCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAGCCGCC
GATGCCTTCTACAAGGCCAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAAATGCGG
CTCCTACGGCTGCAGCGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCT
CTACATGACACAGTTACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTG
GCACGTGACTTCCGCATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGACTGCCCTGGCA
GATTTGGAAGATTGGGAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGC
TACCTGCCTTTTGTGGAGATCTGCATGAAACAACATAACAAATACGAAGCCAAGAAGTAT
GCTTCCCGCGTGGGTCCCGAGCAGAAGGTCAAGGCTTTGCTTCTTGTTGGCGATGTGGCT
CAGGCTGCAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTATTGTCC
CACTGCACGGGAGCCACAGATGGGGCCACAGCTGACAAGATTCAACGGGCCAGGGCACAA
GCCCAGAAGAAG

Protein sequence predicted based on nucleotide sequence

MPWLWTAPHQKESQKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRVGLDGR
AGEGGAGRGWDGQQGSSLLSPSGSSQAASFGKCFLDRFPPDSFVHMCQDLRVLNAVRDYH
IGIPLTYSQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQ
KDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKM
KRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELET
LKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMR
LLRLQRRLEDELGGQFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLTALA
DLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKYEAKKYASRVGPEQKVKALLLVGDVA
QAADVAIEHRNEAELSLVLSHCTGATDGATADKIQRARAQAQKK

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

VPS16

Symbol Nomenclature :

VPS16

SYNONYM :

hVPS16

Designation :

vacuolar protein sorting protein 16|vacuolar protein sorting-associated protein 16 homolog

Full Nomenclature :

vacuolar protein sorting 16 homolog (S. cerevisiae)

GENEID :

64601

Locus Tag :

RP11-12M19.2

HGNC :

14584

MIM :

608550

Vega :

OTTHUMG00000031714

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00513933      ---------------------------ATGCCCTGGCTCTGGACTGCTCCCCACCAGAAA
NM_022575.3       ATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCCTTTTACCGGAAATAT
NM_080413.2       ATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCCTTTTACCGGAAATAT
                                             . .* * ** :**  .*   * .**. .*.:*:

HsCD00513933      GAGAGCCAGAAGGCGGACGAGTACCTGCGGGAGATCCAGGAGCTGGGCCAGCTGACCCAG
NM_022575.3       GAGCTGTACAGCATGGACTGGGACCTGAAGGAGGAACTCAGGGATTGCCTGGTGGCTGCT
NM_080413.2       GAGCTGTACAGCATGGACTGGGACCTGAAGGAGGAACTCAGGGATTGCCTGGTGGCTGCT
                  ***.   * *. . **** .* *****..****.:.*: ..* :  ***:* **.*  . 

HsCD00513933      GCCGTGCAGCAGTGCATTGAGGCTGCAGGACATGAGCACCAGCCAGACATGCAG------
NM_022575.3       GCACCCTATGGGGGCCCCATTGCACTGCTGAGGAACCCCTGGCGGAAGGAGAAAGCTGCT
NM_080413.2       GCACCCTATGGGGGCCCCATTGCACTGCTGAGGAACCCCTGGCGGAAGGAGAAAGCTGCT
                  **.    *  .* **.  .: **:  .  ... .* *.* .** ..* .:*.*.      

HsCD00513933      ------------------------------------------------------------
NM_022575.3       AGTGTGAGGCCAGTGCTCGATATATACTCTGCTTCCGGCATGCCTCTGGCCAGCCTGCTG
NM_080413.2       AGTGTGAGGCCAGTGCTCGATATATACTCTGCTTCCGGCATGCCTCTGGCCAGCCTGCTG
                                                                              

HsCD00513933      ---AAGAGTCTGCTCAGGGTTGGCCTGGATGGCCGGGCCGGGGAGGGTGGGGCTGGGAGG
NM_022575.3       TGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTCAGCTGAGGAGGAGCTGCTCTGTGTG
NM_080413.2       TGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTCAGCTGAGGAGGAGCTGCTCTGTGTG
                     ******  .* *. ***   *****.  *   .** *.*****.   *    * . *

HsCD00513933      GGGTGGGATGGGCAGCAGGGAAGCTCTCTCCTATCGCCCTCTGGCTCTTCTCAGGCCGCC
NM_022575.3       CAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTCCGGAGACACTTCAGC
NM_080413.2       CAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTCCGGAGACACTTCAGC
                   .* ..*****  .  :.  ..  *.*   **: .     .   *   : :**   *. *

HsCD00513933      TCCTTCGGAAAGTGTTTCCTGGACAGATTTCCACCCGACAGCTTCGTGCACATGTGTCAG
NM_022575.3       ATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGATGCCCGGATCTTTCACACTGAGTTT
NM_080413.2       ATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGATGCCCGGATCTTTCACACTGAGTTT
                  :    *..:.*.    ***:*.**.*. *** . . *.*.* :** * ****   .  : 

HsCD00513933      GACCTGCGTGTGCTCAATGCTGTTCGGGACTATCACATCGGGATCCCGCTCACCTATAGC
NM_022575.3       GGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTCAGTGCCAATGTGGGT
NM_080413.2       GGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTCAGTGCCAATGTGGGT
                  *.     *:***  **:    . : ***.* * *.*:**.  .**.    **.  : .* 

HsCD00513933      CAATATAAGCAG------------------------------------------------
NM_022575.3       GACCTCAAACTCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGTGCACCCTCCTGCTGG
NM_080413.2       GACCTCAAACTCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGTGCACCCTCCTGCTGG
                   *. : **.*:                                                 

HsCD00513933      ------------------------------------------------------------
NM_022575.3       ACTGTGCTGTGCCAGGACCGAGTGGCACACATTCTTCTGGCTGTGGGGCCTGACCTTTAC
NM_080413.2       ACTGTGCTGTGCCAGGACCGAGTGGCACACATTCTTCTGGCTGTGGGGCCTGACCTTTAC
                                                                              

HsCD00513933      ------------------------------------------------------------
NM_022575.3       CTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCCCCCTGGCCTGGCCCCAGGAGTAAGC
NM_080413.2       CTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCCCCCTGGCCTGGCCCCAGGAGTAAGC
                                                                              

HsCD00513933      ------------------------------------------------------------
NM_022575.3       AGCTTCCTACAGATGGCTGTCTCCTTCACCTACCGACACCTGGCACTCTTCACAGACACA
NM_080413.2       AGCTTCCTACAGATGGCTGTCTCCTTCACCTACCGACACCTGGCACTCTTCACAGACACA
                                                                              

HsCD00513933      ------------------------------------------------------------
NM_022575.3       GGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAGCTATGTGAGTTCAACTGCAAC
NM_080413.2       GGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAGCTATGTGAGTTCAACTGCAAC
                                                                              

HsCD00513933      ------------------------------------------------------------
NM_022575.3       ATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGTAGCAAGGAGAGGGCC
NM_080413.2       ATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGTAGCAAGGAGAGGGCC
                                                                              

HsCD00513933      ------------------------------------------------------------
NM_022575.3       GTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCACCCGAGAGCATCCAG
NM_080413.2       GTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCACCCGAGAGCATCCAA
                                                                              

HsCD00513933      ------------------------------------------------------------
NM_022575.3       TTTGTGCTGGATGAGGACTCCTACCTGGTGCCTGAGCTCGATGGGGTCCGCATCTTCTCC
NM_080413.2       TATAAGCAG---------------------------------------------------
                                                                              

HsCD00513933      ------------------------------------------------------------
NM_022575.3       CGCAGCACCCACGAGTTCCTGCATGAGGTTCCAGCGGCCAGCGAGGAAATCTTCAAAATT
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00513933      ------------------------------------------------------------
NM_022575.3       GCCTCAATGGCCCCCGGGGCGCTGCTCCTGGAGGCTCAGAAGGAGTATGAGAAAGAGAGC
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00513933      ------------------------------------------------------------
NM_022575.3       CAGAAGGCGGACGAGTACCTGCGGGAGATCCAGGAGCTGGGCCAGCTGACCCAGGCCGTG
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00513933      ------------------------------------------------------------
NM_022575.3       CAGCAGTGCATTGAGGCTGCAGGACATGAGCACCAGCCAGACATGCAGAAGAGTCTGCTC
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00513933      ------------------------------------------------------------
NM_022575.3       AGGGCCGCCTCCTTCGGAAAGTGTTTCCTGGACAGATTTCCACCCGACAGCTTCGTGCAC
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00513933      ------------------------------------------------------------
NM_022575.3       ATGTGTCAGGACCTGCGTGTGCTCAATGCTGTTCGGGACTATCACATCGGGATCCCGCTC
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00513933      ---------------------CTCACCATCCAGGTGCTGCTGGACAGGCTCGTGTTGCGG
NM_022575.3       ACCTATAGCCAATATAAGCAGCTCACCATCCAGGTGCTGCTGGACAGGCTCGTGTTGCGG
NM_080413.2       ---------------------CTCACCATCCAGGTGCTGCTGGACAGGCTCGTGTTGCGG
                                       ***************************************

HsCD00513933      AGACTTTACCCCCTGGCCATCCAGATATGCGAGTACTTGCGCCTTCCTGAAGTACAGGGC
NM_022575.3       AGACTTTACCCCCTGGCCATCCAGATATGCGAGTACTTGCGCCTTCCTGAAGTACAGGGC
NM_080413.2       AGACTTTACCCCCTGGCCATCCAGATATGCGAGTACTTGCGCCTTCCTGAAGTACAGGGC
                  ************************************************************

HsCD00513933      GTCAGCAGGATCCTGGCCCACTGGGCCTGCTACAAGGTGCAACAGAAGGATGTCTCAGAT
NM_022575.3       GTCAGCAGGATCCTGGCCCACTGGGCCTGCTACAAGGTGCAACAGAAGGATGTCTCAGAT
NM_080413.2       GTCAGCAGGATCCTGGCCCACTGGGCCTGCTACAAGGTGCAACAGAAGGATGTCTCAGAT
                  ************************************************************

HsCD00513933      GAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGGGACACGCCTGGTGTCTCTTACTCC
NM_022575.3       GAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGGGACACGCCTGGTGTCTCTTACTCC
NM_080413.2       GAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGGGACACGCCTGGTGTCTCTTACTCC
                  ************************************************************

HsCD00513933      GACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGCTGGCCATCAAGCTGCTGGAG
NM_022575.3       GACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGCTGGCCATCAAGCTGCTGGAG
NM_080413.2       GACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGCTGGCCATCAAGCTGCTGGAG
                  ************************************************************

HsCD00513933      TATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATGAAGAGGAGCAAACTG
NM_022575.3       TATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATGAAGAGGAGCAAACTG
NM_080413.2       TATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATGAAGAGGAGCAAACTG
                  ************************************************************

HsCD00513933      GCACTAAGCAAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTCACGGTGTTGCTGCAC
NM_022575.3       GCACTAAGCAAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTCACGGTGTTGCTGCAC
NM_080413.2       GCACTAAGCAAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTCACGGTGTTGCTGCAC
                  ************************************************************

HsCD00513933      CTGAAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGGAATCAGCCCATGGCC
NM_022575.3       CTGAAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGGAATCAGCCCATGGCC
NM_080413.2       CTGAAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGGAATCAGCCCATGGCC
                  ************************************************************

HsCD00513933      CTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGAGCTAGAGACGCTGAAGGACCTTTAC
NM_022575.3       CTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGAGCTAGAGACGCTGAAGGACCTTTAC
NM_080413.2       CTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGAGCTAGAGACGCTGAAGGACCTTTAC
                  ************************************************************

HsCD00513933      AATCAGGATGACAATCACCAGGAATTGGGCAGCTTCCACATCCGAGCCAGCTATGCTGCA
NM_022575.3       AATCAGGATGACAATCACCAGGAATTGGGCAGCTTCCACATCCGAGCCAGCTATGCTGCA
NM_080413.2       AATCAGGATGACAATCACCAGGAATTGGGCAGCTTCCACATCCGAGCCAGCTATGCTGCA
                  ************************************************************

HsCD00513933      GAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAGCCGCCGATGCCTTCTACAAG
NM_022575.3       GAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAGCCGCCGATGCCTTCTACAAG
NM_080413.2       GAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAGCCGCCGATGCCTTCTACAAG
                  ************************************************************

HsCD00513933      GCCAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAAATGCGGCTCCTACGGCTGCAG
NM_022575.3       GCCAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAAATGCGGCTCCTACGGCTGCAG
NM_080413.2       GCCAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAAATGCGGCTCCTACGGCTGCAG
                  ************************************************************

HsCD00513933      CGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCTCTACATGACACAGTT
NM_022575.3       CGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCTCTACATGACACAGTT
NM_080413.2       CGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCTCTACATGACACAGTT
                  ************************************************************

HsCD00513933      ACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTGGCACGTGACTTCCGC
NM_022575.3       ACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTGGCACGTGACTTCCGC
NM_080413.2       ACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTGGCACGTGACTTCCGC
                  ************************************************************

HsCD00513933      ATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGACTGCCCTGGCAGATTTGGAAGATTGG
NM_022575.3       ATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGACTGCCCTGGCAGATTTGGAAGATTGG
NM_080413.2       ATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGACTGCCCTGGCAGATTTGGAAGATTGG
                  ************************************************************

HsCD00513933      GAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGCTACCTGCCTTTTGTG
NM_022575.3       GAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGCTACCTGCCTTTTGTG
NM_080413.2       GAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGCTACCTGCCTTTTGTG
                  ************************************************************

HsCD00513933      GAGATCTGCATGAAACAACATAACAAATACGAAGCCAAGAAGTATGCTTCCCGCGTGGGT
NM_022575.3       GAGATCTGCATGAAACAACATAACAAATACGAAGCCAAGAAGTATGCTTCCCGCGTGGGT
NM_080413.2       GAGATCTGCATGAAACAACATAACAAATACGAAGCCAAGAAGTATGCTTCCCGCGTGGGT
                  ************************************************************

HsCD00513933      CCCGAGCAGAAGGTCAAGGCTTTGCTTCTTGTTGGCGATGTGGCTCAGGCTGCAGATGTG
NM_022575.3       CCCGAGCAGAAGGTCAAGGCTTTGCTTCTTGTTGGCGATGTGGCTCAGGCTGCAGATGTG
NM_080413.2       CCCGAGCAGAAGGTCAAGGCTTTGCTTCTTGTTGGCGATGTGGCTCAGGCTGCAGATGTG
                  ************************************************************

HsCD00513933      GCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTATTGTCCCACTGCACGGGAGCC
NM_022575.3       GCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTATTGTCCCACTGCACGGGAGCC
NM_080413.2       GCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTATTGTCCCACTGCACGGGAGCC
                  ************************************************************

HsCD00513933      ACAGATGGGGCCACAGCTGACAAGATTCAACGGGCCAGGGCACAAGCCCAGAAGAAG---
NM_022575.3       ACAGATGGGGCCACAGCTGACAAGATTCAACGGGCCAGGGCACAAGCCCAGAAGAAGTGA
NM_080413.2       ACAGATGGGGCCACAGCTGACAAGATTCAACGGGCCAGGGCACAAGCCCAGAAGAAGTGA
                  *********************************************************