DNASU Plasmid | EIF3D (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: EIF3D

Gene Name: eukaryotic translation initiation factor 3, subunit D

Original Clone ID:

ccsbBroadEn_01983

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC080515.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513952

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1645nts

Open reading frame: 1 to 1645

Reference Sequence Alignment

ATGGCAAAGTTCATGACACCCGTGATCCAGGACAACCCCTCAGGCTGGGGTCCCTGTGCG
GTTCCCGAGCAGTTTCGGGATATGCCCTACCAGCCGTTCAGCAAAGGAGATCGGCTAGGA
AAGGTTGCAGACTGGACAGGAGCCACATACCAAGATAAGAGGTACACAAATAAGTACTCC
TCTCAGTTTGGTGGTGGAAGTCAATATGCTTATTTCCATGAGGAGGATGAAAGTAGCTTC
CAGCTGGTGGATACAGCGCGCACACAGAAGACGGCCTACCAGCGGAATCGAATGAGATTT
GCCCAGAGGAACCTCCGCAGAGACAAAGATCGTCGGAACATGTTGCAGTTCAACCTGCAG
ATCCTGCCTAAGAGTGCCAAACAGAAAGAGAGAGAACGCATTCGACTGCAGAAAAAGTTC
CAGAAACAATTTGGGGTTAGGCAGAAATGGGATCAGAAATCACAGAAACCCCGAGACTCT
TCAGTTGAAGTTCGTAGTGATTGGGAAGTGAAAGAGGAAATGGATTTTCCTCAGTTGATG
AAGATGCGCTACTTGGAAGTATCAGAGCCACAGGACATTGAGTGTTGTGGGGCCCTAGAA
TACTACGACAAAGCCTTTGACCGCATCACCACGAGGAGTGAGAAGCCACTGCGGAGCATC
AAGCGCATCTTCCACACTGTCACCACCACAGACGACCCTGTCATCCGCAAGCTGGCAAAA
ACTCAGGGGAATGTGTTTGCCACTGATGCCATCCTGGCCACGCTGATGAGCTGTACCCGC
TCAGTGTATTCCTGGGATATTGTCGTCCAGAGAGTTGGGTCCAAACTCTTCTTTGACAAG
AGAGACAACTCTGACTTTGACCTCCTGACAGTGAGTGAGACTGCCAATGAGCCCCCTCAA
GATGAAGGTAATTCCTTCAATTCACCCCGCAACCTGGCCATGGAGGCAACCTACATCAAC
CACAATTTCTCCCAGCAGTGCTTGAGAATGGGGAAGGAAAGATACAACTTCCCCAACCCA
AACCCGTTTGTGGAGGACGACATGGATAAGAATGAAATCGCCTCTGTTGCGTACCGTTAC
CGCAGGTGGAAGCTTGGAGATGATATTGACCTTATTGTCCGTTGTGAGCACGATGGCGTC
ATGACTGGAGCCAACGGGGAAGTGTCCTTCATCAACATCAAGACACTCAATGAGTGGGAT
TCCAGGCACTGTAATGGCGTTGACTGGCGTCAGAAGCTGGACTCTCAGCGAGGGGCTGTC
ATTGCCACGGAGCTGAAGAACAACAGCTACAAGTTGGCCCGGTGGACCTGCTGTGCTTTG
CTGGCTGGATCTGAGTACCTCAAGCTTGGTTATGTGTCTCGGTACCACGTGAAAGACTCC
TCACGCCACGTCATCCTAGGCACCCAGCAGTTCAAGCCTAATGAGTTTGCCAGCCAGATC
AACCTGAGCGTGGAGAATGCCTGGGGCATTTTACGCTGCGTCATTGACATCTGCATGAAG
CTGGAGGAGGGCAAATACCTCATCCTCAAGGACCCCAACAAGCAGGTCATCCGTGTCTAC
AGCCTCCCTGATGGCACCTTCAGCTCTGATGAAGATGAGGAGGAAGAGGAGGAGGAAGAA
GAGGAAGAAGAAGAGGAAGAAACT

Protein sequence predicted based on nucleotide sequence

MAKFMTPVIQDNPSGWGPCAVPEQFRDMPYQPFSKGDRLGKVADWTGATYQDKRYTNKYS
SQFGGGSQYAYFHEEDESSFQLVDTARTQKTAYQRNRMRFAQRNLRRDKDRRNMLQFNLQ
ILPKSAKQKERERIRLQKKFQKQFGVRQKWDQKSQKPRDSSVEVRSDWEVKEEMDFPQLM
KMRYLEVSEPQDIECCGALEYYDKAFDRITTRSEKPLRSIKRIFHTVTTTDDPVIRKLAK
TQGNVFATDAILATLMSCTRSVYSWDIVVQRVGSKLFFDKRDNSDFDLLTVSETANEPPQ
DEGNSFNSPRNLAMEATYINHNFSQQCLRMGKERYNFPNPNPFVEDDMDKNEIASVAYRY
RRWKLGDDIDLIVRCEHDGVMTGANGEVSFINIKTLNEWDSRHCNGVDWRQKLDSQRGAV
IATELKNNSYKLARWTCCALLAGSEYLKLGYVSRYHVKDSSRHVILGTQQFKPNEFASQI
NLSVENAWGILRCVIDICMKLEEGKYLILKDPNKQVIRVYSLPDGTFSSDEDEEEEEEEE
EEEEEEET

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

EIF3D

Symbol Nomenclature :

EIF3D

SYNONYM :

EIF3S7; eIF3-p66; eIF3-zeta

Designation :

Full Nomenclature :

eukaryotic translation initiation factor 3, subunit D

GENEID :

8664

Locus Tag :

RP5-1119A7.12-003

GenBank Accession :

DQ894308

HGNC :

3278

MIM :

603915

Vega :

OTTHUMG00000150599

Target GenBank:

DQ894308

Reference Sequence Alignment

  

HsCD00513952      ATGGCAAAGTTCATGACACCCGTGATCCAGGACAACCCCTCAGGCTGGGGTCCCTGTGCG
NM_003753.3       ATGGCAAAGTTCATGACACCCGTGATCCAGGACAACCCCTCAGGCTGGGGTCCCTGTGCG
                  ************************************************************

HsCD00513952      GTTCCCGAGCAGTTTCGGGATATGCCCTACCAGCCGTTCAGCAAAGGAGATCGGCTAGGA
NM_003753.3       GTTCCCGAGCAGTTTCGGGATATGCCCTACCAGCCGTTCAGCAAAGGAGATCGGCTAGGA
                  ************************************************************

HsCD00513952      AAGGTTGCAGACTGGACAGGAGCCACATACCAAGATAAGAGGTACACAAATAAGTACTCC
NM_003753.3       AAGGTTGCAGACTGGACAGGAGCCACATACCAAGATAAGAGGTACACAAATAAGTACTCC
                  ************************************************************

HsCD00513952      TCTCAGTTTGGTGGTGGAAGTCAATATGCTTATTTCCATGAGGAGGATGAAAGTAGCTTC
NM_003753.3       TCTCAGTTTGGTGGTGGAAGTCAATATGCTTATTTCCATGAGGAGGATGAAAGTAGCTTC
                  ************************************************************

HsCD00513952      CAGCTGGTGGATACAGCGCGCACACAGAAGACGGCCTACCAGCGGAATCGAATGAGATTT
NM_003753.3       CAGCTGGTGGATACAGCGCGCACACAGAAGACGGCCTACCAGCGGAATCGAATGAGATTT
                  ************************************************************

HsCD00513952      GCCCAGAGGAACCTCCGCAGAGACAAAGATCGTCGGAACATGTTGCAGTTCAACCTGCAG
NM_003753.3       GCCCAGAGGAACCTCCGCAGAGACAAAGATCGTCGGAACATGTTGCAGTTCAACCTGCAG
                  ************************************************************

HsCD00513952      ATCCTGCCTAAGAGTGCCAAACAGAAAGAGAGAGAACGCATTCGACTGCAGAAAAAGTTC
NM_003753.3       ATCCTGCCTAAGAGTGCCAAACAGAAAGAGAGAGAACGCATTCGACTGCAGAAAAAGTTC
                  ************************************************************

HsCD00513952      CAGAAACAATTTGGGGTTAGGCAGAAATGGGATCAGAAATCACAGAAACCCCGAGACTCT
NM_003753.3       CAGAAACAATTTGGGGTTAGGCAGAAATGGGATCAGAAATCACAGAAACCCCGAGACTCT
                  ************************************************************

HsCD00513952      TCAGTTGAAGTTCGTAGTGATTGGGAAGTGAAAGAGGAAATGGATTTTCCTCAGTTGATG
NM_003753.3       TCAGTTGAAGTTCGTAGTGATTGGGAAGTGAAAGAGGAAATGGATTTTCCTCAGTTGATG
                  ************************************************************

HsCD00513952      AAGATGCGCTACTTGGAAGTATCAGAGCCACAGGACATTGAGTGTTGTGGGGCCCTAGAA
NM_003753.3       AAGATGCGCTACTTGGAAGTATCAGAGCCACAGGACATTGAGTGTTGTGGGGCCCTAGAA
                  ************************************************************

HsCD00513952      TACTACGACAAAGCCTTTGACCGCATCACCACGAGGAGTGAGAAGCCACTGCGGAGCATC
NM_003753.3       TACTACGACAAAGCCTTTGACCGCATCACCACGAGGAGTGAGAAGCCACTGCGGAGCATC
                  ************************************************************

HsCD00513952      AAGCGCATCTTCCACACTGTCACCACCACAGACGACCCTGTCATCCGCAAGCTGGCAAAA
NM_003753.3       AAGCGCATCTTCCACACTGTCACCACCACAGACGACCCTGTCATCCGCAAGCTGGCAAAA
                  ************************************************************

HsCD00513952      ACTCAGGGGAATGTGTTTGCCACTGATGCCATCCTGGCCACGCTGATGAGCTGTACCCGC
NM_003753.3       ACTCAGGGGAATGTGTTTGCCACTGATGCCATCCTGGCCACGCTGATGAGCTGTACCCGC
                  ************************************************************

HsCD00513952      TCAGTGTATTCCTGGGATATTGTCGTCCAGAGAGTTGGGTCCAAACTCTTCTTTGACAAG
NM_003753.3       TCAGTGTATTCCTGGGATATTGTCGTCCAGAGAGTTGGGTCCAAACTCTTCTTTGACAAG
                  ************************************************************

HsCD00513952      AGAGACAACTCTGACTTTGACCTCCTGACAGTGAGTGAGACTGCCAATGAGCCCCCTCAA
NM_003753.3       AGAGACAACTCTGACTTTGACCTCCTGACAGTGAGTGAGACTGCCAATGAGCCCCCTCAA
                  ************************************************************

HsCD00513952      GATGAAGGTAATTCCTTCAATTCACCCCGCAACCTGGCCATGGAGGCAACCTACATCAAC
NM_003753.3       GATGAAGGTAATTCCTTCAATTCACCCCGCAACCTGGCCATGGAGGCAACCTACATCAAC
                  ************************************************************

HsCD00513952      CACAATTTCTCCCAGCAGTGCTTGAGAATGGGGAAGGAAAGATACAACTTCCCCAACCCA
NM_003753.3       CACAATTTCTCCCAGCAGTGCTTGAGAATGGGGAAGGAAAGATACAACTTCCCCAACCCA
                  ************************************************************

HsCD00513952      AACCCGTTTGTGGAGGACGACATGGATAAGAATGAAATCGCCTCTGTTGCGTACCGTTAC
NM_003753.3       AACCCGTTTGTGGAGGACGACATGGATAAGAATGAAATCGCCTCTGTTGCGTACCGTTAC
                  ************************************************************

HsCD00513952      CGCAGGTGGAAGCTTGGAGATGATATTGACCTTATTGTCCGTTGTGAGCACGATGGCGTC
NM_003753.3       CGCAGGTGGAAGCTTGGAGATGATATTGACCTTATTGTCCGTTGTGAGCACGATGGCGTC
                  ************************************************************

HsCD00513952      ATGACTGGAGCCAACGGGGAAGTGTCCTTCATCAACATCAAGACACTCAATGAGTGGGAT
NM_003753.3       ATGACTGGAGCCAACGGGGAAGTGTCCTTCATCAACATCAAGACACTCAATGAGTGGGAT
                  ************************************************************

HsCD00513952      TCCAGGCACTGTAATGGCGTTGACTGGCGTCAGAAGCTGGACTCTCAGCGAGGGGCTGTC
NM_003753.3       TCCAGGCACTGTAATGGCGTTGACTGGCGTCAGAAGCTGGACTCTCAGCGAGGGGCTGTC
                  ************************************************************

HsCD00513952      ATTGCCACGGAGCTGAAGAACAACAGCTACAAGTTGGCCCGGTGGACCTGCTGTGCTTTG
NM_003753.3       ATTGCCACGGAGCTGAAGAACAACAGCTACAAGTTGGCCCGGTGGACCTGCTGTGCTTTG
                  ************************************************************

HsCD00513952      CTGGCTGGATCTGAGTACCTCAAGCTTGGTTATGTGTCTCGGTACCACGTGAAAGACTCC
NM_003753.3       CTGGCTGGATCTGAGTACCTCAAGCTTGGTTATGTGTCTCGGTACCACGTGAAAGACTCC
                  ************************************************************

HsCD00513952      TCACGCCACGTCATCCTAGGCACCCAGCAGTTCAAGCCTAATGAGTTTGCCAGCCAGATC
NM_003753.3       TCACGCCACGTCATCCTAGGCACCCAGCAGTTCAAGCCTAATGAGTTTGCCAGCCAGATC
                  ************************************************************

HsCD00513952      AACCTGAGCGTGGAGAATGCCTGGGGCATTTTACGCTGCGTCATTGACATCTGCATGAAG
NM_003753.3       AACCTGAGCGTGGAGAATGCCTGGGGCATTTTACGCTGCGTCATTGACATCTGCATGAAG
                  ************************************************************

HsCD00513952      CTGGAGGAGGGCAAATACCTCATCCTCAAGGACCCCAACAAGCAGGTCATCCGTGTCTAC
NM_003753.3       CTGGAGGAGGGCAAATACCTCATCCTCAAGGACCCCAACAAGCAGGTCATCCGTGTCTAC
                  ************************************************************

HsCD00513952      AGCCTCCCTGATGGCACCTTCAGCTCTGATGAAGATGAGGAGGAAGAGGAGGAGGAAGAA
NM_003753.3       AGCCTCCCTGATGGCACCTTCAGCTCTGATGAAGATGAGGAGGAAGAGGAGGAGGAAGAA
                  ************************************************************

HsCD00513952      GAGGAAGAAGAAGAGGAAGAAACT---
NM_003753.3       GAGGAAGAAGAAGAGGAAGAAACTTAA
                  ************************

Pathways

Reactome :

3' -UTR-mediated translational regulation

Reactome :

Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S

Reactome :

Cap-dependent Translation Initiation

Reactome :

Eukaryotic Translation Initiation

Reactome :

Formation of a pool of free 40S subunits

Reactome :

Formation of the ternary complex, and subsequently, the 43S complex

Reactome :

Gene Expression

Reactome :

GTP hydrolysis and joining of the 60S ribosomal subunit

Reactome :

L13a-mediated translational silencing of Ceruloplasmin expression

Reactome :

Metabolism of proteins

Reactome :

Ribosomal scanning and start codon recognition

Reactome :

Translation

Reactome :

Translation initiation complex formation

WikiPathway :

Translation Factors

Protein

HPRD :

04888

Clone

Cloning Information :

606180

Original Clone ID :

ccsbBroadEn_01983

Organism

Species Specific ID:

8664

Genome

Chromosome :

Map Location :

22q13.1

Ensembl :

ENSG00000100353

Reagents

Labome :

EIF3D-antibody