DNASU Plasmid | CPEB1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: CPEB1

Gene Name: cytoplasmic polyadenylation element binding protein 1

Original Clone ID:

ccsbBroadEn_03954

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC050629.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513955

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1684nts

Open reading frame: 1 to 1684

Reference Sequence Alignment

ATGGCGTTCCCGCTGGAAGAAGAAGCAGGAAGGATAAAAGATTGCTGGGACAACCAGGAA
GCACCTGCTCTCTCCACGTGTAGTAATGCCAATATCTTTCGAAGGATAAATGCCATATTG
GATAATTCTCTGGATTTCAGTAGAGTCTGCACTACACCTATAAACCGAGGAATTCATGAT
CATTTGCCAGACTTCCAGGACTCTGAAGAAACAGTTACAAGCAGGATGCTTTTCCCAACC
TCTGCGCAAGAATCTTCCCGTGGCCTCCCAGATGCAAATGACTTGTGCCTTGGCCTGCAG
TCCCTCAGTCTGACAGGCTGGGACCGACCCTGGAGCACCCAGGACTCAGATTCCTCAGCC
CAGAGCAGCACACACTCGGTACTGAGCATGCTCCATAACCCACTGGGAAATGTCCTAGGA
AAACCCCCCTTGAGCTTCCTGCCTCTGGATCCCCTTGGGTCTGACTTGGTGGACAAGTTT
CCAGCACCCTCAGTTAGAGGATCACGCCTGGACACCCGGCCCATCCTGGACTCTCGATCT
AGCAGCCCCTCTGACTCAGACACCAGTGGCTTCAGCTCTGGATCAGATCATCTCTCAGAT
TTGATTTCAAGCCTTCGCATTTCTCCACCTCTGCCCTTCCTGTCTCTGTCAGGGGGTGGT
CCCAGAGACCCTTTAAAGATGGGGGTAGGGTCTCGGATGGACCAAGAGCAAGCTGCTCTT
GCTGCAGTCACTCCCTCCCCAACCAGTGCTTCAAAGAGATGGCCAGGAGCTTCTGTGTGG
CCATCCTGGGACCTCCTCGAAGCTCCCAAAGACCCCTTCAGCATAGAGAGAGAGGCCAGG
CTGCACCGACAAGCTGCAGCTGTGAATGAAGCCACCTGTACCTGGAGTGGCCAGCTTCCT
CCCCGGAACTATAAGAACCCCATCTACTCTTGCAAGGTGTTTCTAGGAGGTGTTCCTTGG
GATATTACAGAAGCTGGATTAGTTAACACCTTCCGTGTTTTTGGCTCTTTGAGTGTGGAG
TGGCCTGGTAAGGATGGCAAGCATCCCCGGTGTCCTCCCAAAGGGTATGTGTATCTGGTC
TTCGAACTAGAGAAGTCTGTCCGATCCTTGCTTCAGGCTTGCTCTCATGACCCGCTGAGC
CCAGATGGCCTGAGTGAATATTATTTCAAGATGTCCAGCCGAAGGATGCGCTGCAAGGAG
GTGCAGGTGATCCCCTGGGTATTAGCCGACAGTAACTTTGTCCGGAGCCCATCTCAGAGG
CTTGACCCCAGCAGGACGGTGTTTGTCGGTGCTCTGCATGGAATGCTAAATGCTGAGGCC
CTGGCAGCCATCTTGAACGACCTATTTGGTGGAGTGGTGTATGCCGGGATTGACACAGAT
AAGCACAAGTATCCCATTGGTTCTGGTCGTGTGACTTTCAATAACCAACGGAGTTACCTG
AAAGCAGTCAGCGCTGCTTTTGTGGAGATCAAAACCACCAAGTTCACAAAGAAGGTTCAG
ATTGACCCCTACCTAGAAGATTCTCTGTGTCATATCTGCAGTTCTCAGCCTGGTCCTTTC
TTCTGTCGAGATCAGGTCTGCTTCAAATACTTCTGCCGGAGCTGCTGGCACTGGCGGCAC
AGCATGGAGGGCCTGCGCCACCACAGCCCCCTGATGCGGAACCAGAAGAACCGAGATTCC
AGC

Protein sequence predicted based on nucleotide sequence

MAFPLEEEAGRIKDCWDNQEAPALSTCSNANIFRRINAILDNSLDFSRVCTTPINRGIHD
HLPDFQDSEETVTSRMLFPTSAQESSRGLPDANDLCLGLQSLSLTGWDRPWSTQDSDSSA
QSSTHSVLSMLHNPLGNVLGKPPLSFLPLDPLGSDLVDKFPAPSVRGSRLDTRPILDSRS
SSPSDSDTSGFSSGSDHLSDLISSLRISPPLPFLSLSGGGPRDPLKMGVGSRMDQEQAAL
AAVTPSPTSASKRWPGASVWPSWDLLEAPKDPFSIEREARLHRQAAAVNEATCTWSGQLP
PRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPRCPPKGYVYLV
FELEKSVRSLLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVRSPSQR
LDPSRTVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYL
KAVSAAFVEIKTTKFTKKVQIDPYLEDSLCHICSSQPGPFFCRDQVCFKYFCRSCWHWRH
SMEGLRHHSPLMRNQKNRDSS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

CPEB1

Symbol Nomenclature :

CPEB1

SYNONYM :

CEBP; CPE-BP1; CPEB; CPEB-1; hCPEB-1

Designation :

CPE-binding protein 1|cytoplasmic polyadenylation element-binding protein 1

Full Nomenclature :

cytoplasmic polyadenylation element binding protein 1

GENEID :

64506

GenBank Accession :

HQ448361

HGNC :

21744

MIM :

607342

Vega :

OTTHUMG00000172877

Target GenBank:

HQ448361

Reference Sequence Alignment

  

HsCD00513955      ATGGCGTTCCCGCTGGAAGAAGAAGCAGGAAGGATAAAAGATTGCTGGGACAACCAGGAA
NM_030594.4       ATGGCGTTCCCGCTGGAAGAAGAAGCAGGAAGGATAAAAGATTGCTGGGACAACCAGGAA
                  ************************************************************

HsCD00513955      GCACCTGCTCTCTCCACGTGTAGTAATGCCAATATCTTTCGAAGGATAAATGCCATATTG
NM_030594.4       GCACCTGCTCTCTCCACGTGTAGTAATGCCAATATCTTTCGAAGGATAAATGCCATATTG
                  ************************************************************

HsCD00513955      GATAATTCTCTGGATTTCAGTAGAGTCTGCACTACACCTATAAACCGAGGAATTCATGAT
NM_030594.4       GATAATTCTCTGGATTTCAGTAGAGTCTGCACTACACCTATAAACCGAGGAATTCATGAT
                  ************************************************************

HsCD00513955      CATTTGCCAGACTTCCAGGACTCTGAAGAAACAGTTACAAGCAGGATGCTTTTCCCAACC
NM_030594.4       CATTTGCCAGACTTCCAGGACTCTGAAGAAACAGTTACAAGCAGGATGCTTTTCCCAACC
                  ************************************************************

HsCD00513955      TCTGCGCAAGAATCTTCCCGTGGCCTCCCAGATGCAAATGACTTGTGCCTTGGCCTGCAG
NM_030594.4       TCTGCGCAAGAATCTTCCCGTGGCCTCCCAGATGCAAATGACTTGTGCCTTGGCCTGCAG
                  ************************************************************

HsCD00513955      TCCCTCAGTCTGACAGGCTGGGACCGACCCTGGAGCACCCAGGACTCAGATTCCTCAGCC
NM_030594.4       TCCCTCAGTCTGACAGGCTGGGACCGACCCTGGAGCACCCAGGACTCAGATTCCTCAGCC
                  ************************************************************

HsCD00513955      CAGAGCAGCACACACTCGGTACTGAGCATGCTCCATAACCCACTGGGAAATGTCCTAGGA
NM_030594.4       CAGAGCAGCACACACTCGGTACTGAGCATGCTCCATAACCCACTGGGAAATGTCCTAGGA
                  ************************************************************

HsCD00513955      AAACCCCCCTTGAGCTTCCTGCCTCTGGATCCCCTTGGGTCTGACTTGGTGGACAAGTTT
NM_030594.4       AAACCCCCCTTGAGCTTCCTGCCTCTGGATCCCCTTGGGTCTGACTTGGTGGACAAGTTT
                  ************************************************************

HsCD00513955      CCAGCACCCTCAGTTAGAGGATCACGCCTGGACACCCGGCCCATCCTGGACTCTCGATCT
NM_030594.4       CCAGCACCCTCAGTTAGAGGATCACGCCTGGACACCCGGCCCATCCTGGACTCTCGATCT
                  ************************************************************

HsCD00513955      AGCAGCCCCTCTGACTCAGACACCAGTGGCTTCAGCTCTGGATCAGATCATCTCTCAGAT
NM_030594.4       AGCAGCCCCTCTGACTCAGACACCAGTGGCTTCAGCTCTGGATCAGATCATCTCTCAGAT
                  ************************************************************

HsCD00513955      TTGATTTCAAGCCTTCGCATTTCTCCACCTCTGCCCTTCCTGTCTCTGTCAGGGGGTGGT
NM_030594.4       TTGATTTCAAGCCTTCGCATTTCTCCACCTCTGCCCTTCCTGTCTCTGTCAGGGGGTGGT
                  ************************************************************

HsCD00513955      CCCAGAGACCCTTTAAAGATGGGGGTAGGGTCTCGGATGGACCAAGAGCAAGCTGCTCTT
NM_030594.4       CCCAGAGACCCTTTAAAGATGGGGGTAGGGTCTCGGATGGACCAAGAGCAAGCTGCTCTT
                  ************************************************************

HsCD00513955      GCTGCAGTCACTCCCTCCCCAACCAGTGCTTCAAAGAGATGGCCAGGAGCTTCTGTGTGG
NM_030594.4       GCTGCAGTCACTCCCTCCCCAACCAGTGCTTCAAAGAGATGGCCAGGAGCTTCTGTGTGG
                  ************************************************************

HsCD00513955      CCATCCTGGGACCTCCTCGAAGCTCCCAAAGACCCCTTCAGCATAGAGAGAGAGGCCAGG
NM_030594.4       CCATCCTGGGACCTCCTCGAAGCTCCCAAAGACCCCTTCAGCATAGAGAGAGAGGCCAGG
                  ************************************************************

HsCD00513955      CTGCACCGACAAGCTGCAGCTGTGAATGAAGCCACCTGTACCTGGAGTGGCCAGCTTCCT
NM_030594.4       CTGCACCGACAAGCTGCAGCTGTGAATGAAGCCACCTGTACCTGGAGTGGCCAGCTTCCT
                  ************************************************************

HsCD00513955      CCCCGGAACTATAAGAACCCCATCTACTCTTGCAAGGTGTTTCTAGGAGGTGTTCCTTGG
NM_030594.4       CCCCGGAACTATAAGAACCCCATCTACTCTTGCAAGGTGTTTCTAGGAGGTGTTCCTTGG
                  ************************************************************

HsCD00513955      GATATTACAGAAGCTGGATTAGTTAACACCTTCCGTGTTTTTGGCTCTTTGAGTGTGGAG
NM_030594.4       GATATTACAGAAGCTGGATTAGTTAACACCTTCCGTGTTTTTGGCTCTTTGAGTGTGGAG
                  ************************************************************

HsCD00513955      TGGCCTGGTAAGGATGGCAAGCATCCCCGGTGTCCTCCCAAAGGGTATGTGTATCTGGTC
NM_030594.4       TGGCCTGGTAAGGATGGCAAGCATCCCCGGTGTCCTCCCAAAGGGTATGTGTATCTGGTC
                  ************************************************************

HsCD00513955      TTCGAACTAGAGAAGTCTGTCCGATCCTTGCTTCAGGCTTGCTCTCATGACCCGCTGAGC
NM_030594.4       TTCGAACTAGAGAAGTCTGTCCGATCCTTGCTTCAGGCTTGCTCTCATGACCCGCTGAGC
                  ************************************************************

HsCD00513955      CCAGATGGCCTGAGTGAATATTATTTCAAGATGTCCAGCCGAAGGATGCGCTGCAAGGAG
NM_030594.4       CCAGATGGCCTGAGTGAATATTATTTCAAGATGTCCAGCCGAAGGATGCGCTGCAAGGAG
                  ************************************************************

HsCD00513955      GTGCAGGTGATCCCCTGGGTATTAGCCGACAGTAACTTTGTCCGGAGCCCATCTCAGAGG
NM_030594.4       GTGCAGGTGATCCCCTGGGTATTAGCCGACAGTAACTTTGTCCGGAGCCCATCTCAGAGG
                  ************************************************************

HsCD00513955      CTTGACCCCAGCAGGACGGTGTTTGTCGGTGCTCTGCATGGAATGCTAAATGCTGAGGCC
NM_030594.4       CTTGACCCCAGCAGGACGGTGTTTGTCGGTGCTCTGCATGGAATGCTAAATGCTGAGGCC
                  ************************************************************

HsCD00513955      CTGGCAGCCATCTTGAACGACCTATTTGGTGGAGTGGTGTATGCCGGGATTGACACAGAT
NM_030594.4       CTGGCAGCCATCTTGAACGACCTATTTGGTGGAGTGGTGTATGCCGGGATTGACACAGAT
                  ************************************************************

HsCD00513955      AAGCACAAGTATCCCATTGGTTCTGGTCGTGTGACTTTCAATAACCAACGGAGTTACCTG
NM_030594.4       AAGCACAAGTATCCCATTGGTTCTGGTCGTGTGACTTTCAATAACCAACGGAGTTACCTG
                  ************************************************************

HsCD00513955      AAAGCAGTCAGCGCTGCTTTTGTGGAGATCAAAACCACCAAGTTCACAAAGAAGGTTCAG
NM_030594.4       AAAGCAGTCAGCGCTGCTTTTGTGGAGATCAAAACCACCAAGTTCACAAAGAAGGTTCAG
                  ************************************************************

HsCD00513955      ATTGACCCCTACCTAGAAGATTCTCTGTGTCATATCTGCAGTTCTCAGCCTGGTCCTTTC
NM_030594.4       ATTGACCCCTACCTAGAAGATTCTCTGTGTCATATCTGCAGTTCTCAGCCTGGTCCTTTC
                  ************************************************************

HsCD00513955      TTCTGTCGAGATCAGGTCTGCTTCAAATACTTCTGCCGGAGCTGCTGGCACTGGCGGCAC
NM_030594.4       TTCTGTCGAGATCAGGTCTGCTTCAAATACTTCTGCCGGAGCTGCTGGCACTGGCGGCAC
                  ************************************************************

HsCD00513955      AGCATGGAGGGCCTGCGCCACCACAGCCCCCTGATGCGGAACCAGAAGAACCGAGATTCC
NM_030594.4       AGCATGGAGGGCCTGCGCCACCACAGCCCCCTGATGCGGAACCAGAAGAACCGAGATTCC
                  ************************************************************

HsCD00513955      AGC---
NM_030594.4       AGCTAG
                  ***