DNASU Plasmid | ZNF416 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF416

Gene Name: zinc finger protein 416

Original Clone ID:

ccsbBroadEn_03624

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC000130.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00514049

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1783nts

Open reading frame: 1 to 1783

Reference Sequence Alignment

ATGGCGGCGGCCGTGCTTAGGGATTCGACTTCGGTTCCCGTGACTGCAGAAGCTAAACTT
ATGGGCTTTACACAGGGCTGTGTGACCTTTGAGGACGTGGCCATTTACTTCTCCCAGGAA
GAATGGGGGCTCCTTGATGAGGCTCAGAGGCTCCTGTACCGCGATGTGATGCTGGAGAAC
TTTGCACTTATAACTGCGCTGGTTTGTTGGCATGGGATGGAGGATGAAGAGACACCTGAG
CAAAGTGTTTCTGTAGAAGGAGTACCTCAGGTCAGGACTCCAGAGGCCAGTCCATCCACC
CAGAAGATTCAATCCTGTGACATGTGTGTCCCATTCCTGACCGACATTTTGCACCTGACC
GATTTGCCTGGGCAGGAACTATACTTGACTGGGGCATGTGCGGTCTTTCACCAGGACCAG
AAGCATCATAGTGCAGAGAAACCCTTGGAAAGTGACATGGACAAGGCCTCATTTGTGCAG
TGCTGCCTGTTCCATGAGTCAGGAATGCCTTTCACCAGCAGTGAGGTTGGGAAGGACTTC
CTAGCCCCATTGGGCATTCTTCAGCCGCAAGCTATTGCTAACTATGAGAAGCCAAACAAA
ATCAGCAAATGTGAGGAGGCCTTTCATGTTGGAATAAGTCATTACAAGTGGAGTCAATGC
AGGAGAGAGTCCAGCCACAAACACACTTTTTTTCACCCTAGAGTCTGCACTGGAAAAAGG
CTTTATGAATCTAGCAAATGTGGGAAAGCCTGCTGCTGTGAGTGCTCCCTTGTTCAGCTG
CAAAGAGTCCACCCTGGAGAAAGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTTAGC
CAAACCTCTCATCTGAATGATCATCGGAGAATCCACACTGGAGAAAGGCCTTATGTGTGT
GGTCAGTGTGGGAAATCATTTAGCCAAAGAGCCACCCTCATTAAACATCACAGAGTTCAC
ACTGGAGAAAGGCCTTACGAGTGTGGTGAATGTGGGAAATCTTTTAGCCAAAGTTCCAAC
CTTATTGAACATTGCAGAATTCACACTGGAGAAAGGCCTTATGAGTGTGATGAATGTGGA
AAAGCCTTTGGGTCCAAATCCACTCTTGTTCGACACCAGAGAACTCACACAGGAGAAAAG
CCATATGAGTGTGGTGAATGTGGGAAATTATTCAGACAAAGCTTCAGCCTTGTTGTACAC
CAGAGAATTCACACTACAGCAAGGCCTTATGAGTGTGGCCAGTGTGGGAAATCATTTAGC
CTAAAGTGTGGCCTCATTCAGCACCAGTTAATTCACAGTGGAGCTAGGCCCTTTGAGTGT
GATGAGTGCGGAAAATCCTTTAGCCAAAGAACCACCCTCAATAAACACCACAAAGTTCAC
ACTGCAGAAAGGCCTTATGTATGTGGGGAATGTGGGAAAGCTTTTATGTTCAAATCTAAA
CTTGTTAGGCACCAGAGAACTCACACTGGAGAAAGGCCTTTTGAGTGCAGTGAATGTGGG
AAATTTTTTAGACAAAGCTATACCCTCGTTGAACACCAGAAAATTCACACTGGATTAAGG
CCTTACGACTGTGGACAGTGCGGGAAATCCTTTATCCAAAAGTCTAGCCTCATTCAACAC
CAAGTGGTTCACACAGGAGAAAGGCCATATGAGTGTGGCAAATGTGGGAAGTCCTTTACA
CAACACTCTGGCCTCATTCTCCACCGAAAATCTCACACTGTGGAGAGGCCTCGTGACAGC
AGCAAATGTGGAAAACCCTACAGCCCAAGATCTAACATTGTT

Protein sequence predicted based on nucleotide sequence

MAAAVLRDSTSVPVTAEAKLMGFTQGCVTFEDVAIYFSQEEWGLLDEAQRLLYRDVMLEN
FALITALVCWHGMEDEETPEQSVSVEGVPQVRTPEASPSTQKIQSCDMCVPFLTDILHLT
DLPGQELYLTGACAVFHQDQKHHSAEKPLESDMDKASFVQCCLFHESGMPFTSSEVGKDF
LAPLGILQPQAIANYEKPNKISKCEEAFHVGISHYKWSQCRRESSHKHTFFHPRVCTGKR
LYESSKCGKACCCECSLVQLQRVHPGERPYECSECGKSFSQTSHLNDHRRIHTGERPYVC
GQCGKSFSQRATLIKHHRVHTGERPYECGECGKSFSQSSNLIEHCRIHTGERPYECDECG
KAFGSKSTLVRHQRTHTGEKPYECGECGKLFRQSFSLVVHQRIHTTARPYECGQCGKSFS
LKCGLIQHQLIHSGARPFECDECGKSFSQRTTLNKHHKVHTAERPYVCGECGKAFMFKSK
LVRHQRTHTGERPFECSECGKFFRQSYTLVEHQKIHTGLRPYDCGQCGKSFIQKSSLIQH
QVVHTGERPYECGKCGKSFTQHSGLILHRKSHTVERPRDSSKCGKPYSPRSNIV

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF416

Symbol Nomenclature :

ZNF416

Full Nomenclature :

zinc finger protein 416

GENEID :

55659

GenBank Accession :

EU446818

HGNC :

20645

Vega :

OTTHUMG00000183467

Target GenBank:

EU446818

Reference Sequence Alignment

  

HsCD00514049      ATGGCGGCGGCCGTGCTTAGGGATTCGACTTCGGTTCCCGTGACTGCAGAAGCTAAACTT
NM_017879.1       ATGGCGGCGGCCGTGCTTAGGGATTCGACTTCGGTTCCCGTGACTGCAGAAGCTAAACTT
                  ************************************************************

HsCD00514049      ATGGGCTTTACACAGGGCTGTGTGACCTTTGAGGACGTGGCCATTTACTTCTCCCAGGAA
NM_017879.1       ATGGGCTTTACACAGGGCTGTGTGACCTTTGAGGACGTGGCCATTTACTTCTCCCAGGAA
                  ************************************************************

HsCD00514049      GAATGGGGGCTCCTTGATGAGGCTCAGAGGCTCCTGTACCGCGATGTGATGCTGGAGAAC
NM_017879.1       GAATGGGGGCTCCTTGATGAGGCTCAGAGGCTCCTGTACCGCGATGTGATGCTGGAGAAC
                  ************************************************************

HsCD00514049      TTTGCACTTATAACTGCGCTGGTTTGTTGGCATGGGATGGAGGATGAAGAGACACCTGAG
NM_017879.1       TTTGCACTTATAACTGCGCTGGTTTGTTGGCATGGGATGGAGGATGAAGAGACACCTGAG
                  ************************************************************

HsCD00514049      CAAAGTGTTTCTGTAGAAGGAGTACCTCAGGTCAGGACTCCAGAGGCCAGTCCATCCACC
NM_017879.1       CAAAGTGTTTCTGTAGAAGGAGTACCTCAGGTCAGGACTCCAGAGGCCAGTCCATCCACC
                  ************************************************************

HsCD00514049      CAGAAGATTCAATCCTGTGACATGTGTGTCCCATTCCTGACCGACATTTTGCACCTGACC
NM_017879.1       CAGAAGATTCAATCCTGTGACATGTGTGTCCCATTCCTGACCGACATTTTGCACCTGACC
                  ************************************************************

HsCD00514049      GATTTGCCTGGGCAGGAACTATACTTGACTGGGGCATGTGCGGTCTTTCACCAGGACCAG
NM_017879.1       GATTTGCCTGGGCAGGAACTATACTTGACTGGGGCATGTGCGGTCTTTCACCAGGACCAG
                  ************************************************************

HsCD00514049      AAGCATCATAGTGCAGAGAAACCCTTGGAAAGTGACATGGACAAGGCCTCATTTGTGCAG
NM_017879.1       AAGCATCATAGTGCAGAGAAACCCTTGGAAAGTGACATGGACAAGGCCTCATTTGTGCAG
                  ************************************************************

HsCD00514049      TGCTGCCTGTTCCATGAGTCAGGAATGCCTTTCACCAGCAGTGAGGTTGGGAAGGACTTC
NM_017879.1       TGCTGCCTGTTCCATGAGTCAGGAATGCCTTTCACCAGCAGTGAGGTTGGGAAGGACTTC
                  ************************************************************

HsCD00514049      CTAGCCCCATTGGGCATTCTTCAGCCGCAAGCTATTGCTAACTATGAGAAGCCAAACAAA
NM_017879.1       CTAGCCCCATTGGGCATTCTTCAGCCGCAAGCTATTGCTAACTATGAGAAGCCAAACAAA
                  ************************************************************

HsCD00514049      ATCAGCAAATGTGAGGAGGCCTTTCATGTTGGAATAAGTCATTACAAGTGGAGTCAATGC
NM_017879.1       ATCAGCAAATGTGAGGAGGCCTTTCATGTTGGAATAAGTCATTACAAGTGGAGTCAATGC
                  ************************************************************

HsCD00514049      AGGAGAGAGTCCAGCCACAAACACACTTTTTTTCACCCTAGAGTCTGCACTGGAAAAAGG
NM_017879.1       AGGAGAGAGTCCAGCCACAAACACACTTTTTTTCACCCTAGAGTCTGCACTGGAAAAAGG
                  ************************************************************

HsCD00514049      CTTTATGAATCTAGCAAATGTGGGAAAGCCTGCTGCTGTGAGTGCTCCCTTGTTCAGCTG
NM_017879.1       CTTTATGAATCTAGCAAATGTGGGAAAGCCTGCTGCTGTGAGTGCTCCCTTGTTCAGCTG
                  ************************************************************

HsCD00514049      CAAAGAGTCCACCCTGGAGAAAGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTTAGC
NM_017879.1       CAAAGAGTCCACCCTGGAGAAAGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTTAGC
                  ************************************************************

HsCD00514049      CAAACCTCTCATCTGAATGATCATCGGAGAATCCACACTGGAGAAAGGCCTTATGTGTGT
NM_017879.1       CAAACCTCTCATCTGAATGATCATCGGAGAATCCACACTGGAGAAAGGCCTTATGTGTGT
                  ************************************************************

HsCD00514049      GGTCAGTGTGGGAAATCATTTAGCCAAAGAGCCACCCTCATTAAACATCACAGAGTTCAC
NM_017879.1       GGTCAGTGTGGGAAATCATTTAGCCAAAGAGCCACCCTCATTAAACATCACAGAGTTCAC
                  ************************************************************

HsCD00514049      ACTGGAGAAAGGCCTTACGAGTGTGGTGAATGTGGGAAATCTTTTAGCCAAAGTTCCAAC
NM_017879.1       ACTGGAGAAAGGCCTTACGAGTGTGGTGAATGTGGGAAATCTTTTAGCCAAAGTTCCAAC
                  ************************************************************

HsCD00514049      CTTATTGAACATTGCAGAATTCACACTGGAGAAAGGCCTTATGAGTGTGATGAATGTGGA
NM_017879.1       CTTATTGAACATTGCAGAATTCACACTGGAGAAAGGCCTTATGAGTGTGATGAATGTGGA
                  ************************************************************

HsCD00514049      AAAGCCTTTGGGTCCAAATCCACTCTTGTTCGACACCAGAGAACTCACACAGGAGAAAAG
NM_017879.1       AAAGCCTTTGGGTCCAAATCCACTCTTGTTCGACACCAGAGAACTCACACAGGAGAAAAG
                  ************************************************************

HsCD00514049      CCATATGAGTGTGGTGAATGTGGGAAATTATTCAGACAAAGCTTCAGCCTTGTTGTACAC
NM_017879.1       CCATATGAGTGTGGTGAATGTGGGAAATTATTCAGACAAAGCTTCAGCCTTGTTGTACAC
                  ************************************************************

HsCD00514049      CAGAGAATTCACACTACAGCAAGGCCTTATGAGTGTGGCCAGTGTGGGAAATCATTTAGC
NM_017879.1       CAGAGAATTCACACTACAGCAAGGCCTTATGAGTGTGGCCAGTGTGGGAAATCATTTAGC
                  ************************************************************

HsCD00514049      CTAAAGTGTGGCCTCATTCAGCACCAGTTAATTCACAGTGGAGCTAGGCCCTTTGAGTGT
NM_017879.1       CTAAAGTGTGGCCTCATTCAGCACCAGTTAATTCACAGTGGAGCTAGGCCCTTTGAGTGT
                  ************************************************************

HsCD00514049      GATGAGTGCGGAAAATCCTTTAGCCAAAGAACCACCCTCAATAAACACCACAAAGTTCAC
NM_017879.1       GATGAGTGCGGAAAATCCTTTAGCCAAAGAACCACCCTCAATAAACACCACAAAGTTCAC
                  ************************************************************

HsCD00514049      ACTGCAGAAAGGCCTTATGTATGTGGGGAATGTGGGAAAGCTTTTATGTTCAAATCTAAA
NM_017879.1       ACTGCAGAAAGGCCTTATGTATGTGGGGAATGTGGGAAAGCTTTTATGTTCAAATCTAAA
                  ************************************************************

HsCD00514049      CTTGTTAGGCACCAGAGAACTCACACTGGAGAAAGGCCTTTTGAGTGCAGTGAATGTGGG
NM_017879.1       CTTGTTAGGCACCAGAGAACTCACACTGGAGAAAGGCCTTTTGAGTGCAGTGAATGTGGG
                  ************************************************************

HsCD00514049      AAATTTTTTAGACAAAGCTATACCCTCGTTGAACACCAGAAAATTCACACTGGATTAAGG
NM_017879.1       AAATTTTTTAGACAAAGCTATACCCTCGTTGAACACCAGAAAATTCACACTGGATTAAGG
                  ************************************************************

HsCD00514049      CCTTACGACTGTGGACAGTGCGGGAAATCCTTTATCCAAAAGTCTAGCCTCATTCAACAC
NM_017879.1       CCTTACGACTGTGGACAGTGCGGGAAATCCTTTATCCAAAAGTCTAGCCTCATTCAACAC
                  ************************************************************

HsCD00514049      CAAGTGGTTCACACAGGAGAAAGGCCATATGAGTGTGGCAAATGTGGGAAGTCCTTTACA
NM_017879.1       CAAGTGGTTCACACAGGAGAAAGGCCATATGAGTGTGGCAAATGTGGGAAGTCCTTTACA
                  ************************************************************

HsCD00514049      CAACACTCTGGCCTCATTCTCCACCGAAAATCTCACACTGTGGAGAGGCCTCGTGACAGC
NM_017879.1       CAACACTCTGGCCTCATTCTCCACCGAAAATCTCACACTGTGGAGAGGCCTCGTGACAGC
                  ************************************************************

HsCD00514049      AGCAAATGTGGAAAACCCTACAGCCCAAGATCTAACATTGTT---
NM_017879.1       AGCAAATGTGGAAAACCCTACAGCCCAAGATCTAACATTGTTTAA
                  ******************************************