DNASU Plasmid | PTPN9 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: PTPN9

Gene Name: protein tyrosine phosphatase, non-receptor type 9

Original Clone ID:

ccsbBroadEn_01343

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC010863.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00514081

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1779nts

Open reading frame: 1 to 1779

Reference Sequence Alignment

ATGGAGCCCGCGACCGCGCCCCGGCCCGACATGGCGCCGGAGCTGACCCCGGAGGAGGAG
CAGGCTACCAAGCAGTTTCTCGAAGAGATTAACAAGTGGACAGTTCAGTACAATGTTTCC
CCGCTGTCTTGGAATGTGGCTGTCAAGTTCCTCATGGCAAGGAAGTTTGATGTGCTCCGT
GCCATAGAATTGTTCCACTCCTACAGAGAAACTCGAAGGAAGGAAGGCATTGTAAAGCTG
AAACCTCATGAGGAACCTCTTCGTTCTGAGATCCTCAGTGGAAAATTCACCATCTTAAAT
GTTCGGGACCCAACAGGAGCCTCCATTGCCCTCTTTACTGCCAGGTTGCATCATCCCCAC
AAGTCAGTCCAACATGTGGTACTTCAGGCTCTGTTTTACTTGCTAGACAGAGCTGTGGAT
AGCTTTGAAACTCAGAGGAATGGACTGGTGTTTATCTATGACATGTGTGGTTCTAATTAT
GCCAACTTTGAGCTGGATCTTGGCAAGAAAGTCCTAAACCTGCTGAAGGGAGCATTTCCA
GCTCGTTTGAAGAAGGTGCTGATTGTGGGGGCACCCATATGGTTCCGAGTGCCCTATTCC
ATCATCAGTCTCCTCCTGAAGGACAAAGTCCGGGAGAGGATTCAAATATTAAAGACATCT
GAGGTCACGCAGCATCTGCCCAGGGAGTGTCTTCCAGAAAACCTGGGTGGGTACGTCAAA
ATTGATCTCGCCACTTGGAATTTCCAGTTCCTACCCCAGGTGAACGGCCACCCAGATCCC
TTCGATGAGATCATCCTGTTCTCCCTCCCTCCTGCCTTAGACTGGGACTCAGTACATGTT
CCAGGTCCCCATGCTATGACCATCCAAGAGTTGGTGGACTATGTTAATGCCAGGCAAAAG
CAAGGAATCTATGAGGAATATGAAGACATTCGTCGTGAGAACCCTGTTGGCACTTTCCAC
TGTTCCATGTCTCCAGGAAACCTAGAGAAAAACCGTTATGGGGATGTACCCTGCCTGGAC
CAAACTAGAGTGAAGCTAACAAAGCGAAGTGGCCATACTCAGACAGATTACATCAATGCC
AGTTTCATGGATGGCTACAAGCAGAAGAATGCTTACATTGGCACACAAGGTCCTTTGGAG
AATACCTATCGTGATTTCTGGCTCATGGTATGGGAGCAAAAAGTCTTGGTGATTGTCATG
ACCACCCGCTTTGAGGAAGGCGGCAGGAGAAAGTGTGGCCAGTACTGGCCTTTAGAAAAA
GACTCTCGGATCCGATTTGGCTTCCTCACAGTGACCAATCTAGGCGTGGAGAACATGAAT
CATTATAAGAAAACAACGCTAGAAATTCACAACACAGAGGAACGGCAGAAACGCCAGGTG
ACCCACTTCCAGTTCTTGAGCTGGCCAGACTATGGTGTCCCTTCCTCAGCAGCTTCCCTC
ATTGACTTCTTGAGAGTGGTCAGAAACCAGCAGAGTCTGGCTGTGAGCAACATGGGAGCA
CGCTCCAAAGGGCAGTGCCCTGAGCCACCCATTGTGGTCCATTGCAGTGCAGGCATTGGC
AGGACAGGTACCTTCTGCTCACTGGACATCTGCCTGGCACAGCTGGAGGAGCTTGGCACC
CTTAATGTGTTCCAGACGGTGTCACGCATGAGGACCCAGAGGGCCTTCAGCATCCAGACC
CCTGAGCAGTACTATTTTTGCTACAAGGCCATCCTGGAGTTCGCAGAGAAGGAGGGCATG
GTATCCTCTGGCCAAAACCTGCTGGCCGTGGAGAGTCAG

Protein sequence predicted based on nucleotide sequence

MEPATAPRPDMAPELTPEEEQATKQFLEEINKWTVQYNVSPLSWNVAVKFLMARKFDVLR
AIELFHSYRETRRKEGIVKLKPHEEPLRSEILSGKFTILNVRDPTGASIALFTARLHHPH
KSVQHVVLQALFYLLDRAVDSFETQRNGLVFIYDMCGSNYANFELDLGKKVLNLLKGAFP
ARLKKVLIVGAPIWFRVPYSIISLLLKDKVRERIQILKTSEVTQHLPRECLPENLGGYVK
IDLATWNFQFLPQVNGHPDPFDEIILFSLPPALDWDSVHVPGPHAMTIQELVDYVNARQK
QGIYEEYEDIRRENPVGTFHCSMSPGNLEKNRYGDVPCLDQTRVKLTKRSGHTQTDYINA
SFMDGYKQKNAYIGTQGPLENTYRDFWLMVWEQKVLVIVMTTRFEEGGRRKCGQYWPLEK
DSRIRFGFLTVTNLGVENMNHYKKTTLEIHNTEERQKRQVTHFQFLSWPDYGVPSSAASL
IDFLRVVRNQQSLAVSNMGARSKGQCPEPPIVVHCSAGIGRTGTFCSLDICLAQLEELGT
LNVFQTVSRMRTQRAFSIQTPEQYYFCYKAILEFAEKEGMVSSGQNLLAVESQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

PTPN9

Symbol Nomenclature :

PTPN9

SYNONYM :

MEG2; PTPMEG2

Designation :

PTPase MEG2|PTPase-MEG2|protein-tyrosine phosphatase MEG2|tyrosine-protein phosphatase non-receptor type 9

Full Nomenclature :

protein tyrosine phosphatase, non-receptor type 9

GENEID :

5780

GenBank Accession :

DQ890986

HGNC :

9661

MIM :

600768

Vega :

OTTHUMG00000142838

Target GenBank:

DQ890986

Reference Sequence Alignment

  

HsCD00514081      ATGGAGCCCGCGACCGCGCCCCGGCCCGACATGGCGCCGGAGCTGACCCCGGAGGAGGAG
NM_002833.3       ATGGAGCCCGCGACCGCGCCCCGGCCCGACATGGCGCCGGAGCTGACCCCGGAGGAGGAG
                  ************************************************************

HsCD00514081      CAGGCTACCAAGCAGTTTCTCGAAGAGATTAACAAGTGGACAGTTCAGTACAATGTTTCC
NM_002833.3       CAGGCTACCAAGCAGTTTCTCGAAGAGATTAACAAGTGGACAGTTCAGTACAATGTTTCC
                  ************************************************************

HsCD00514081      CCGCTGTCTTGGAATGTGGCTGTCAAGTTCCTCATGGCAAGGAAGTTTGATGTGCTCCGT
NM_002833.3       CCGCTGTCTTGGAATGTGGCTGTCAAGTTCCTCATGGCAAGGAAGTTTGATGTGCTCCGT
                  ************************************************************

HsCD00514081      GCCATAGAATTGTTCCACTCCTACAGAGAAACTCGAAGGAAGGAAGGCATTGTAAAGCTG
NM_002833.3       GCCATAGAATTGTTCCACTCCTACAGAGAAACTCGAAGGAAGGAAGGCATTGTAAAGCTG
                  ************************************************************

HsCD00514081      AAACCTCATGAGGAACCTCTTCGTTCTGAGATCCTCAGTGGAAAATTCACCATCTTAAAT
NM_002833.3       AAACCTCATGAGGAACCTCTTCGTTCTGAGATCCTCAGTGGAAAATTCACCATCTTAAAT
                  ************************************************************

HsCD00514081      GTTCGGGACCCAACAGGAGCCTCCATTGCCCTCTTTACTGCCAGGTTGCATCATCCCCAC
NM_002833.3       GTTCGGGACCCAACAGGAGCCTCCATTGCCCTCTTTACTGCCAGGTTGCATCATCCCCAC
                  ************************************************************

HsCD00514081      AAGTCAGTCCAACATGTGGTACTTCAGGCTCTGTTTTACTTGCTAGACAGAGCTGTGGAT
NM_002833.3       AAGTCAGTCCAACATGTGGTACTTCAGGCTCTGTTTTACTTGCTAGACAGAGCTGTGGAT
                  ************************************************************

HsCD00514081      AGCTTTGAAACTCAGAGGAATGGACTGGTGTTTATCTATGACATGTGTGGTTCTAATTAT
NM_002833.3       AGCTTTGAAACTCAGAGGAATGGACTGGTGTTTATCTATGACATGTGTGGTTCTAATTAT
                  ************************************************************

HsCD00514081      GCCAACTTTGAGCTGGATCTTGGCAAGAAAGTCCTAAACCTGCTGAAGGGAGCATTTCCA
NM_002833.3       GCCAACTTTGAGCTGGATCTTGGCAAGAAAGTCCTAAACCTGCTGAAGGGAGCATTTCCA
                  ************************************************************

HsCD00514081      GCTCGTTTGAAGAAGGTGCTGATTGTGGGGGCACCCATATGGTTCCGAGTGCCCTATTCC
NM_002833.3       GCTCGTTTGAAGAAGGTGCTGATTGTGGGGGCACCCATATGGTTCCGAGTGCCCTATTCC
                  ************************************************************

HsCD00514081      ATCATCAGTCTCCTCCTGAAGGACAAAGTCCGGGAGAGGATTCAAATATTAAAGACATCT
NM_002833.3       ATCATCAGTCTCCTCCTGAAGGACAAAGTCCGGGAGAGGATTCAAATATTAAAGACATCT
                  ************************************************************

HsCD00514081      GAGGTCACGCAGCATCTGCCCAGGGAGTGTCTTCCAGAAAACCTGGGTGGGTACGTCAAA
NM_002833.3       GAGGTCACGCAGCATCTGCCCAGGGAGTGTCTTCCAGAAAACCTGGGTGGGTACGTCAAA
                  ************************************************************

HsCD00514081      ATTGATCTCGCCACTTGGAATTTCCAGTTCCTACCCCAGGTGAACGGCCACCCAGATCCC
NM_002833.3       ATTGATCTCGCCACTTGGAATTTCCAGTTCCTACCCCAGGTGAACGGCCACCCAGATCCC
                  ************************************************************

HsCD00514081      TTCGATGAGATCATCCTGTTCTCCCTCCCTCCTGCCTTAGACTGGGACTCAGTACATGTT
NM_002833.3       TTCGATGAGATCATCCTGTTCTCCCTCCCTCCTGCCTTAGACTGGGACTCAGTACATGTT
                  ************************************************************

HsCD00514081      CCAGGTCCCCATGCTATGACCATCCAAGAGTTGGTGGACTATGTTAATGCCAGGCAAAAG
NM_002833.3       CCAGGTCCCCATGCTATGACCATCCAAGAGTTGGTGGACTATGTTAATGCCAGGCAAAAG
                  ************************************************************

HsCD00514081      CAAGGAATCTATGAGGAATATGAAGACATTCGTCGTGAGAACCCTGTTGGCACTTTCCAC
NM_002833.3       CAAGGAATCTATGAGGAATATGAAGACATTCGTCGTGAGAACCCTGTTGGCACTTTCCAC
                  ************************************************************

HsCD00514081      TGTTCCATGTCTCCAGGAAACCTAGAGAAAAACCGTTATGGGGATGTACCCTGCCTGGAC
NM_002833.3       TGTTCCATGTCTCCAGGAAACCTAGAGAAAAACCGTTATGGGGATGTACCCTGCCTGGAC
                  ************************************************************

HsCD00514081      CAAACTAGAGTGAAGCTAACAAAGCGAAGTGGCCATACTCAGACAGATTACATCAATGCC
NM_002833.3       CAAACTAGAGTGAAGCTAACAAAGCGAAGTGGCCATACTCAGACAGATTACATCAATGCC
                  ************************************************************

HsCD00514081      AGTTTCATGGATGGCTACAAGCAGAAGAATGCTTACATTGGCACACAAGGTCCTTTGGAG
NM_002833.3       AGTTTCATGGATGGCTACAAGCAGAAGAATGCTTACATTGGCACACAAGGTCCTTTGGAG
                  ************************************************************

HsCD00514081      AATACCTATCGTGATTTCTGGCTCATGGTATGGGAGCAAAAAGTCTTGGTGATTGTCATG
NM_002833.3       AATACCTATCGTGATTTCTGGCTCATGGTATGGGAGCAAAAAGTCTTGGTGATTGTCATG
                  ************************************************************

HsCD00514081      ACCACCCGCTTTGAGGAAGGCGGCAGGAGAAAGTGTGGCCAGTACTGGCCTTTAGAAAAA
NM_002833.3       ACCACCCGCTTTGAGGAAGGCGGCAGGAGAAAGTGTGGCCAGTACTGGCCTTTAGAAAAA
                  ************************************************************

HsCD00514081      GACTCTCGGATCCGATTTGGCTTCCTCACAGTGACCAATCTAGGCGTGGAGAACATGAAT
NM_002833.3       GACTCTCGGATCCGATTTGGCTTCCTCACAGTGACCAATCTAGGCGTGGAGAACATGAAT
                  ************************************************************

HsCD00514081      CATTATAAGAAAACAACGCTAGAAATTCACAACACAGAGGAACGGCAGAAACGCCAGGTG
NM_002833.3       CATTATAAGAAAACAACGCTAGAAATTCACAACACAGAGGAACGGCAGAAACGCCAGGTG
                  ************************************************************

HsCD00514081      ACCCACTTCCAGTTCTTGAGCTGGCCAGACTATGGTGTCCCTTCCTCAGCAGCTTCCCTC
NM_002833.3       ACCCACTTCCAGTTCTTGAGCTGGCCAGACTATGGTGTCCCTTCCTCAGCAGCTTCCCTC
                  ************************************************************

HsCD00514081      ATTGACTTCTTGAGAGTGGTCAGAAACCAGCAGAGTCTGGCTGTGAGCAACATGGGAGCA
NM_002833.3       ATTGACTTCTTGAGAGTGGTCAGAAACCAGCAGAGTCTGGCTGTGAGCAACATGGGAGCA
                  ************************************************************

HsCD00514081      CGCTCCAAAGGGCAGTGCCCTGAGCCACCCATTGTGGTCCATTGCAGTGCAGGCATTGGC
NM_002833.3       CGCTCCAAAGGGCAGTGCCCTGAGCCACCCATTGTGGTCCATTGCAGTGCAGGCATTGGC
                  ************************************************************

HsCD00514081      AGGACAGGTACCTTCTGCTCACTGGACATCTGCCTGGCACAGCTGGAGGAGCTTGGCACC
NM_002833.3       AGGACAGGTACCTTCTGCTCACTGGACATCTGCCTGGCACAGCTGGAGGAGCTTGGCACC
                  ************************************************************

HsCD00514081      CTTAATGTGTTCCAGACGGTGTCACGCATGAGGACCCAGAGGGCCTTCAGCATCCAGACC
NM_002833.3       CTTAATGTGTTCCAGACGGTGTCACGCATGAGGACCCAGAGGGCCTTCAGCATCCAGACC
                  ************************************************************

HsCD00514081      CCTGAGCAGTACTATTTTTGCTACAAGGCCATCCTGGAGTTCGCAGAGAAGGAGGGCATG
NM_002833.3       CCTGAGCAGTACTATTTTTGCTACAAGGCCATCCTGGAGTTCGCAGAGAAGGAGGGCATG
                  ************************************************************

HsCD00514081      GTATCCTCTGGCCAAAACCTGCTGGCCGTGGAGAGTCAG---
NM_002833.3       GTATCCTCTGGCCAAAACCTGCTGGCCGTGGAGAGTCAGTAA
                  ***************************************