DNASU Plasmid | ZNF530 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF530

Gene Name: zinc finger protein 530

Original Clone ID:

ccsbBroadEn_10058

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC060865.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00514099

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1798nts

Open reading frame: 1 to 1798

Reference Sequence Alignment

ATGGCGGCGGCACTGAGGGCCCCGACCCAGCAGGTTTTTGTAGCCTTTGAGGATGTGGCC
ATTTACTTCTCCCAGGAGGAGTGGGAGCTCCTTGATGAGATGCAGAGGCTCCTGTACCGC
GATGTGATGCTGGAGAACTTTGCAGTTATGGCATCCCTAGGTTGCTGGTGTGGAGCAGTA
GATGAGGGGACGCCTTCTGCAGAGAGCGTTTCTGTGGAAGAACTGTCACAGGGCAGGACT
CCAAAGGCAGATACATCCACTGATAAGAGTCACCCCTGTGAGATTTGTACCCCAGTCCTG
AGAGACATTTTACAAATGATTGAGCTCCAAGCCTCACCCTGTGGACAGAAATTGTACTTG
GGTGGAGCACCAAGAGATTTCTGGATGAGTTCAAACCTTCACCAGCTCCAGAAGCTTGAT
AATGGAGAGAAGCTCTTTAAAGTGGATGGGGACCAGGCCTCATTTATGATGAACTGCAGG
TTCCATGTGTCAGGAAAACCCTTCACGTTTGGGGAAGTCGGGAGGGACTTTTCAGCCACC
TCAGGACTTCTCCAGCATCAGGTGACTCCCACCATTGAGAGACCACACAGCAGGATTAGA
CACTTGAGAGTTCCCACTGGACGAAAGCCTCTCAAATACACTGAATCCAGGAAATCTTTT
AGAGAGAAATCTGTATTCATTCAACACCAAAGAGCTGACTCTGGAGAAAGGCCTTACAAG
TGCAGTGAATGTGGGAAATCCTTTAGTCAAAGTTCTGGCTTTCTTCGACACAGGAAAGCA
CACGGTAGAACAAGGACTCATGAATGTAGTGAATGTGGGAAATCATTTAGTCGCAAAACT
CACCTAACTCAACACCAAAGAGTTCACACTGGAGAAAGGCCTTATGACTGCAGTGAATGT
GGCAAATCCTTTCGCCAGGTATCTGTCCTCATTCAACATCAACGAGTTCACACTGGAGAA
AGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTTAGCCACAGCACTAACCTCTATCGT
CACAGGAGTGCCCACACTAGCACAAGGCCTTATGAGTGCAGTGAATGTGGAAAATCCTTT
AGCCATAGCACTAACCTCTTTCGACACTGGAGAGTTCACACTGGAGTAAGGCCTTATGAG
TGTAGTGAATGTGGGAAAGCATTTAGTTGCAATATCTACCTTATTCACCACCAAAGATTT
CACACTGGAGAAAGACCTTATGTGTGCAGTGAATGTGGGAAATCATTTGGCCAGAAATCT
GTCCTCATTCAACACCAAAGAGTTCACACTGGAGAAAGGCCTTATGAGTGCAGTGAATGT
GGGAAAGTTTTTAGCCAAAGCTCTGGCCTCTTTCGACACAGAAGAGCTCACACTAAAACA
AAGCCTTATGAGTGCAGTGAATGTGAAAAATCATTTAGTTGCAAAACTGACCTCATTCGA
CACCAGACAGTTCACACTGGAGAAAGGCCTTATGAGTGCAGTGTATGTGGGAAATCTTTT
ATCCGAAAAACCCACCTCATTCGACACCAGACTGTTCACACTAATGAAAGGCCTTATGAG
TGCGATGAATGTGGGAAATCCTATAGCCAAAGCTCTGCCCTCCTTCAGCATAGGAGAGTT
CACACTGGAGAAAGGCCTTATGAGTGCAGAGAATGTGGGAAATCTTTTACCCGCAAAAAT
CACCTCATTCAACACAAGACAGTTCACACTGGAGAAAGGCCTTATGAATGCAGTGAATGT
GGAAAATCCTTTAGCCAAAGCTCTGGCCTCTTAAGACACAGAAGAGTTCATGTGCAG

Protein sequence predicted based on nucleotide sequence

MAAALRAPTQQVFVAFEDVAIYFSQEEWELLDEMQRLLYRDVMLENFAVMASLGCWCGAV
DEGTPSAESVSVEELSQGRTPKADTSTDKSHPCEICTPVLRDILQMIELQASPCGQKLYL
GGAPRDFWMSSNLHQLQKLDNGEKLFKVDGDQASFMMNCRFHVSGKPFTFGEVGRDFSAT
SGLLQHQVTPTIERPHSRIRHLRVPTGRKPLKYTESRKSFREKSVFIQHQRADSGERPYK
CSECGKSFSQSSGFLRHRKAHGRTRTHECSECGKSFSRKTHLTQHQRVHTGERPYDCSEC
GKSFRQVSVLIQHQRVHTGERPYECSECGKSFSHSTNLYRHRSAHTSTRPYECSECGKSF
SHSTNLFRHWRVHTGVRPYECSECGKAFSCNIYLIHHQRFHTGERPYVCSECGKSFGQKS
VLIQHQRVHTGERPYECSECGKVFSQSSGLFRHRRAHTKTKPYECSECEKSFSCKTDLIR
HQTVHTGERPYECSVCGKSFIRKTHLIRHQTVHTNERPYECDECGKSYSQSSALLQHRRV
HTGERPYECRECGKSFTRKNHLIQHKTVHTGERPYECSECGKSFSQSSGLLRHRRVHVQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF530

Symbol Nomenclature :

ZNF530

Full Nomenclature :

zinc finger protein 530

GENEID :

348327

GenBank Accession :

EU446633

HGNC :

29297

Vega :

OTTHUMG00000183469

Target GenBank:

EU446633

Reference Sequence Alignment

  

HsCD00514099      ATGGCGGCGGCACTGAGGGCCCCGACCCAGCAGGTTTTTGTAGCCTTTGAGGATGTGGCC
NM_020880.3       ATGGCGGCGGCACTGAGGGCCCCGACCCAGCAGGTTTTTGTAGCCTTTGAGGATGTGGCC
                  ************************************************************

HsCD00514099      ATTTACTTCTCCCAGGAGGAGTGGGAGCTCCTTGATGAGATGCAGAGGCTCCTGTACCGC
NM_020880.3       ATTTACTTCTCCCAGGAGGAGTGGGAGCTCCTTGATGAGATGCAGAGGCTCCTGTACCGC
                  ************************************************************

HsCD00514099      GATGTGATGCTGGAGAACTTTGCAGTTATGGCATCCCTAGGTTGCTGGTGTGGAGCAGTA
NM_020880.3       GATGTGATGCTGGAGAACTTTGCAGTTATGGCATCCCTAGGTTGCTGGTGTGGAGCAGTA
                  ************************************************************

HsCD00514099      GATGAGGGGACGCCTTCTGCAGAGAGCGTTTCTGTGGAAGAACTGTCACAGGGCAGGACT
NM_020880.3       GATGAGGGGACGCCTTCTGCAGAGAGCGTTTCTGTGGAAGAACTGTCACAGGGCAGGACT
                  ************************************************************

HsCD00514099      CCAAAGGCAGATACATCCACTGATAAGAGTCACCCCTGTGAGATTTGTACCCCAGTCCTG
NM_020880.3       CCAAAGGCAGATACATCCACTGATAAGAGTCACCCCTGTGAGATTTGTACCCCAGTCCTG
                  ************************************************************

HsCD00514099      AGAGACATTTTACAAATGATTGAGCTCCAAGCCTCACCCTGTGGACAGAAATTGTACTTG
NM_020880.3       AGAGACATTTTACAAATGATTGAGCTCCATGCCTCACCCTGTGGACAGAAATTGTACTTG
                  *****************************:******************************

HsCD00514099      GGTGGAGCACCAAGAGATTTCTGGATGAGTTCAAACCTTCACCAGCTCCAGAAGCTTGAT
NM_020880.3       GGTGGAGCATCAAGAGATTTCTGGATGAGTTCAAACCTTCACCAGCTCCAGAAGCTTGAT
                  ********* **************************************************

HsCD00514099      AATGGAGAGAAGCTCTTTAAAGTGGATGGGGACCAGGCCTCATTTATGATGAACTGCAGG
NM_020880.3       AATGGAGAGAAGCTCTTTAAAGTGGATGGGGACCAGGCCTCATTTATGATGAACTGCAGG
                  ************************************************************

HsCD00514099      TTCCATGTGTCAGGAAAACCCTTCACGTTTGGGGAAGTCGGGAGGGACTTTTCAGCCACC
NM_020880.3       TTCCATGTGTCAGGAAAACCCTTCACGTTTGGGGAAGTCGGGAGGGACTTTTCAGCCACC
                  ************************************************************

HsCD00514099      TCAGGACTTCTCCAGCATCAGGTGACTCCCACCATTGAGAGACCACACAGCAGGATTAGA
NM_020880.3       TCAGGACTTCTCCAGCATCAGGTGACTCCCACCATTGAGAGACCACACAGCAGGATTAGA
                  ************************************************************

HsCD00514099      CACTTGAGAGTTCCCACTGGACGAAAGCCTCTCAAATACACTGAATCCAGGAAATCTTTT
NM_020880.3       CACTTGAGAGTTCCCACTGGACGAAAGCCTCTCAAATACACTGAATCCAGGAAATCTTTT
                  ************************************************************

HsCD00514099      AGAGAGAAATCTGTATTCATTCAACACCAAAGAGCTGACTCTGGAGAAAGGCCTTACAAG
NM_020880.3       AGAGAGAAATCTGTATTCATTCAACACCAAAGAGCTGACTCTGGAGAAAGGCCTTACAAG
                  ************************************************************

HsCD00514099      TGCAGTGAATGTGGGAAATCCTTTAGTCAAAGTTCTGGCTTTCTTCGACACAGGAAAGCA
NM_020880.3       TGCAGTGAATGTGGGAAATCCTTTAGTCAAAGTTCTGGCTTTCTTCGACACAGGAAAGCA
                  ************************************************************

HsCD00514099      CACGGTAGAACAAGGACTCATGAATGTAGTGAATGTGGGAAATCATTTAGTCGCAAAACT
NM_020880.3       CACGGTAGAACAAGGACTCATGAATGTAGTGAATGTGGGAAATCATTTAGTCGCAAAACT
                  ************************************************************

HsCD00514099      CACCTAACTCAACACCAAAGAGTTCACACTGGAGAAAGGCCTTATGACTGCAGTGAATGT
NM_020880.3       CACCTAACTCAACACCAAAGAGTTCACACTGGAGAAAGGCCTTATGACTGCAGTGAATGT
                  ************************************************************

HsCD00514099      GGCAAATCCTTTCGCCAGGTATCTGTCCTCATTCAACATCAACGAGTTCACACTGGAGAA
NM_020880.3       GGCAAATCCTTTCGCCAGGTATCTGTCCTCATTCAACATCAACGAGTTCACACTGGAGAA
                  ************************************************************

HsCD00514099      AGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTTAGCCACAGCACTAACCTCTATCGT
NM_020880.3       AGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTTAGCCACAGCACTAACCTCTATCGT
                  ************************************************************

HsCD00514099      CACAGGAGTGCCCACACTAGCACAAGGCCTTATGAGTGCAGTGAATGTGGAAAATCCTTT
NM_020880.3       CACAGGAGTGCCCACACTAGCACAAGGCCTTATGAGTGCAGTGAATGTGGAAAATCCTTT
                  ************************************************************

HsCD00514099      AGCCATAGCACTAACCTCTTTCGACACTGGAGAGTTCACACTGGAGTAAGGCCTTATGAG
NM_020880.3       AGCCATAGCACTAACCTCTTTCGACACTGGAGAGTTCACACTGGAGTAAGGCCTTATGAG
                  ************************************************************

HsCD00514099      TGTAGTGAATGTGGGAAAGCATTTAGTTGCAATATCTACCTTATTCACCACCAAAGATTT
NM_020880.3       TGTAGTGAATGTGGGAAAGCATTTAGTTGCAATATCTACCTTATTCACCACCAAAGATTT
                  ************************************************************

HsCD00514099      CACACTGGAGAAAGACCTTATGTGTGCAGTGAATGTGGGAAATCATTTGGCCAGAAATCT
NM_020880.3       CACACTGGAGAAAGACCTTATGTGTGCAGTGAATGTGGGAAATCATTTGGCCAGAAATCT
                  ************************************************************

HsCD00514099      GTCCTCATTCAACACCAAAGAGTTCACACTGGAGAAAGGCCTTATGAGTGCAGTGAATGT
NM_020880.3       GTCCTCATTCAACACCAAAGAGTTCACACTGGAGAAAGGCCTTATGAGTGCAGTGAATGT
                  ************************************************************

HsCD00514099      GGGAAAGTTTTTAGCCAAAGCTCTGGCCTCTTTCGACACAGAAGAGCTCACACTAAAACA
NM_020880.3       GGGAAAGTTTTTAGCCAAAGCTCTGGCCTCTTTCGACACAGAAGAGCTCACACTAAAACA
                  ************************************************************

HsCD00514099      AAGCCTTATGAGTGCAGTGAATGTGAAAAATCATTTAGTTGCAAAACTGACCTCATTCGA
NM_020880.3       AAGCCTTATGAGTGCAGTGAATGTGAAAAATCATTTAGTTGCAAAACTGACCTCATTCGA
                  ************************************************************

HsCD00514099      CACCAGACAGTTCACACTGGAGAAAGGCCTTATGAGTGCAGTGTATGTGGGAAATCTTTT
NM_020880.3       CACCAGACAGTTCACACTGGAGAAAGGCCTTATGAGTGCAGTGTATGTGGGAAATCTTTT
                  ************************************************************

HsCD00514099      ATCCGAAAAACCCACCTCATTCGACACCAGACTGTTCACACTAATGAAAGGCCTTATGAG
NM_020880.3       ATCCGAAAAACCCACCTCATTCGACACCAGACTGTTCACACTAATGAAAGGCCTTATGAG
                  ************************************************************

HsCD00514099      TGCGATGAATGTGGGAAATCCTATAGCCAAAGCTCTGCCCTCCTTCAGCATAGGAGAGTT
NM_020880.3       TGCGATGAATGTGGGAAATCCTATAGCCAAAGCTCTGCCCTCCTTCAGCATAGGAGAGTT
                  ************************************************************

HsCD00514099      CACACTGGAGAAAGGCCTTATGAGTGCAGAGAATGTGGGAAATCTTTTACCCGCAAAAAT
NM_020880.3       CACACTGGAGAAAGGCCTTATGAGTGCAGAGAATGTGGGAAATCTTTTACCCGCAAAAAT
                  ************************************************************

HsCD00514099      CACCTCATTCAACACAAGACAGTTCACACTGGAGAAAGGCCTTATGAATGCAGTGAATGT
NM_020880.3       CACCTCATTCAACACAAGACAGTTCACACTGGAGAAAGGCCTTATGAATGCAGTGAATGT
                  ************************************************************

HsCD00514099      GGAAAATCCTTTAGCCAAAGCTCTGGCCTCTTAAGACACAGAAGAGTTCATGTGCAG---
NM_020880.3       GGAAAATCCTTTAGCCAAAGCTCTGGCCTCTTAAGACACAGAAGAGTTCATGTGCAGTGA
                  *********************************************************