DNASU Plasmid | ZNF571 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF571

Gene Name: zinc finger protein 571

Original Clone ID:

ccsbBroadEn_08268

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC114479.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00514196

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1828nts

Open reading frame: 1 to 1828

Reference Sequence Alignment

ATGCCCCACTTGTTGGTGACTTTCAGGGATGTGGCCATTGACTTCTCTCAGGAGGAATGG
GAATGCCTGGACCCTGCTCAGAGGGACTTGTACAGGGATGTGATGTTGGAGAACTACAGC
AACCTGATCTCATTGGACTTGGAATCCAGTTGTGTGACCAAAAAGTTATCTCCAGAAAAG
GAAATTTATGAAATGGAATCACTCCAGTGGGAGAATATGGGGAAACGTATCAACCATCAC
CTTCAATACAATGGTCTTGGAGACAATATGGAGTGCAAAGGCAACTTAGAGGGTCAAGAA
GCAAGTCAGGAAGGGCTTTACATGTGTGTCAAAATTACCTGTGAAGAAAAGGCCACTGAA
AGTCATTCAACCTCTTCTACTTTTCATCGGATAATTCCTACCAAGGAAAAATTGTACAAA
TGTAAGGAATGCAGACAAGGTTTCAGCTACCTGTCATGCCTTATTCAACATGAGGAAAAT
CATAATATAGAAAAATGCTCTGAAGTTAAGAAACACAGGAATACCTTTAGCAAAAAGCCA
AGCTATATTCAACATCAGAGAATTCAGACTGGTGAGAAACCTTATGAGTGTATGGAATGT
GGAAAGGCCTTTGGTCGTACTTCTGATCTCATTCAACATCAGAAAATTCATACTAATGAA
AAACCTTATCAGTGTAACGCATGTGGGAAAGCTTTTATTCGTGGTTCACAGCTCACTGAA
CATCAGAGAGTTCATACAGGAGAGAAACCATATGATTGTAAGAAATGTGGAAAAGCCTTT
AGTTATTGTTCACAATATACTCTTCATCAGAGAATTCATAGTGGTGAAAAACCCTATGAA
TGTAAGGATTGTGGGAAGGCCTTTATTCTTGGCTCTCAACTTACTTACCATCAGAGAATT
CATAGTGGTGAGAAACCTTATGAGTGTAAGGAATGTGGAAAGGCCTTTATTCTTGGTTCA
CACCTTACATACCATCAGAGAGTTCATACTGGTGAAAAGCCTTACATATGTAAAGAATGT
GGGAAAGCCTTTTTATGTGCCTCCCAACTGAATGAACATCAGAGAATTCATACAGGAGAG
AAACCCTATGAATGTAAAGAATGCGGGAAGACCTTTTTTCGTGGCTCACAACTTACTTAC
CACCTGAGAGTTCATTCAGGTGAGAGACCTTATAAATGCAAAGAATGTGGGAAAGCCTTT
ATTTCTAATTCTAATCTTATTCAACATCAAAGAATTCATACCGGAGAGAAGCCCTACAAA
TGTAAGGAATGTGGAAAGGCCTTTATTTGTGGCAAACAACTTAGTGAACATCAGAGAATT
CATACAGGTGAGAAACCCTTTGAATGTAAGGAATGTGGAAAGGCCTTTATTCGTGTTGCA
TATCTTACTCAACATGAGAAAATTCATGGTGAGAAACATTATGAATGTAAGGAATGTGGG
AAGACCTTTGTACGTGCTACACAACTTACATATCATCAAAGAATTCATACAGGTGAAAAG
CCCTACAAATGTAAGGAATGTGACAAGGCCTTTATTTATGGCTCACAACTTAGTGAACAT
CAGAGAATTCACAGAGGTGAAAAACCTTATGAATGTAAACAGTGTGGGAAGGCTTTTATT
CGTGGCTCACACCTTACTGAACATCTGAGAACTCATACTGGAGAGAAACCCTATGAATGT
AAGGAATGTGGGAGGGCCTTTAGTCGTGGCTCAGAACATACTCTGCATCAAAGGATCCAT
ACTGGTGAGAAACCCTATACATGTGTCCAGTGTGGTAAAGACTTTAGATGTCCTTCACAA
CTTACTCAACATACAAGGCTTCATAAT

Protein sequence predicted based on nucleotide sequence

MPHLLVTFRDVAIDFSQEEWECLDPAQRDLYRDVMLENYSNLISLDLESSCVTKKLSPEK
EIYEMESLQWENMGKRINHHLQYNGLGDNMECKGNLEGQEASQEGLYMCVKITCEEKATE
SHSTSSTFHRIIPTKEKLYKCKECRQGFSYLSCLIQHEENHNIEKCSEVKKHRNTFSKKP
SYIQHQRIQTGEKPYECMECGKAFGRTSDLIQHQKIHTNEKPYQCNACGKAFIRGSQLTE
HQRVHTGEKPYDCKKCGKAFSYCSQYTLHQRIHSGEKPYECKDCGKAFILGSQLTYHQRI
HSGEKPYECKECGKAFILGSHLTYHQRVHTGEKPYICKECGKAFLCASQLNEHQRIHTGE
KPYECKECGKTFFRGSQLTYHLRVHSGERPYKCKECGKAFISNSNLIQHQRIHTGEKPYK
CKECGKAFICGKQLSEHQRIHTGEKPFECKECGKAFIRVAYLTQHEKIHGEKHYECKECG
KTFVRATQLTYHQRIHTGEKPYKCKECDKAFIYGSQLSEHQRIHRGEKPYECKQCGKAFI
RGSHLTEHLRTHTGEKPYECKECGRAFSRGSEHTLHQRIHTGEKPYTCVQCGKDFRCPSQ
LTQHTRLHN

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF571

Symbol Nomenclature :

ZNF571

Full Nomenclature :

zinc finger protein 571

GENEID :

51276

Locus Tag :

HSPC059

GenBank Accession :

EU446803

HGNC :

25000

Vega :

OTTHUMG00000182016

Target GenBank:

EU446803

Reference Sequence Alignment

  

HsCD00514196      ATGCCCCACTTGTTGGTGACTTTCAGGGATGTGGCCATTGACTTCTCTCAGGAGGAATGG
NM_016536.3       ATGCCCCACTTGTTGGTGACTTTCAGGGATGTGGCCATTGACTTCTCTCAGGAGGAATGG
                  ************************************************************

HsCD00514196      GAATGCCTGGACCCTGCTCAGAGGGACTTGTACAGGGATGTGATGTTGGAGAACTACAGC
NM_016536.3       GAATGCCTGGACCCTGCTCAGAGGGACTTGTACAGGGATGTGATGTTGGAGAACTACAGC
                  ************************************************************

HsCD00514196      AACCTGATCTCATTGGACTTGGAATCCAGTTGTGTGACCAAAAAGTTATCTCCAGAAAAG
NM_016536.3       AACCTGATCTCATTGGACTTGGAATCCAGTTGTGTGACCAAAAAGTTATCTCCAGAAAAG
                  ************************************************************

HsCD00514196      GAAATTTATGAAATGGAATCACTCCAGTGGGAGAATATGGGGAAACGTATCAACCATCAC
NM_016536.3       GAAATTTATGAAATGGAATCACTCCAGTGGGAGAATATGGGGAAACGTATCAACCATCAC
                  ************************************************************

HsCD00514196      CTTCAATACAATGGTCTTGGAGACAATATGGAGTGCAAAGGCAACTTAGAGGGTCAAGAA
NM_016536.3       CTTCAATACAATGGTCTTGGAGACAATATGGAGTGCAAAGGCAACTTAGAGGGTCAAGAA
                  ************************************************************

HsCD00514196      GCAAGTCAGGAAGGGCTTTACATGTGTGTCAAAATTACCTGTGAAGAAAAGGCCACTGAA
NM_016536.3       GCAAGTCAGGAAGGGCTTTACATGTGTGTCAAAATTACCTGTGAAGAAAAGGCCACTGAA
                  ************************************************************

HsCD00514196      AGTCATTCAACCTCTTCTACTTTTCATCGGATAATTCCTACCAAGGAAAAATTGTACAAA
NM_016536.3       AGTCATTCAACCTCTTCTACTTTTCATCGGATAATTCCTACCAAGGAAAAATTGTACAAA
                  ************************************************************

HsCD00514196      TGTAAGGAATGCAGACAAGGTTTCAGCTACCTGTCATGCCTTATTCAACATGAGGAAAAT
NM_016536.3       TGTAAGGAATGCAGACAAGGTTTCAGCTACCTGTCATGCCTTATTCAACATGAGGAAAAT
                  ************************************************************

HsCD00514196      CATAATATAGAAAAATGCTCTGAAGTTAAGAAACACAGGAATACCTTTAGCAAAAAGCCA
NM_016536.3       CATAATATAGAAAAATGCTCTGAAGTTAAGAAACACAGGAATACCTTTAGCAAAAAGCCA
                  ************************************************************

HsCD00514196      AGCTATATTCAACATCAGAGAATTCAGACTGGTGAGAAACCTTATGAGTGTATGGAATGT
NM_016536.3       AGCTATATTCAACATCAGAGAATTCAGACTGGTGAGAAACCTTATGAGTGTATGGAATGT
                  ************************************************************

HsCD00514196      GGAAAGGCCTTTGGTCGTACTTCTGATCTCATTCAACATCAGAAAATTCATACTAATGAA
NM_016536.3       GGAAAGGCCTTTGGTCGTACTTCTGATCTCATTCAACATCAGAAAATTCATACTAATGAA
                  ************************************************************

HsCD00514196      AAACCTTATCAGTGTAACGCATGTGGGAAAGCTTTTATTCGTGGTTCACAGCTCACTGAA
NM_016536.3       AAACCTTATCAGTGTAACGCATGTGGGAAAGCTTTTATTCGTGGTTCACAGCTCACTGAA
                  ************************************************************

HsCD00514196      CATCAGAGAGTTCATACAGGAGAGAAACCATATGATTGTAAGAAATGTGGAAAAGCCTTT
NM_016536.3       CATCAGAGAGTTCATACAGGAGAGAAACCATATGAATGTAAGAAATGTGGAAAAGCCTTT
                  ***********************************:************************

HsCD00514196      AGTTATTGTTCACAATATACTCTTCATCAGAGAATTCATAGTGGTGAAAAACCCTATGAA
NM_016536.3       AGTTATTGTTCACAATATACTCTTCATCAGAGAATTCATAGTGGTGAAAAACCCTATGAA
                  ************************************************************

HsCD00514196      TGTAAGGATTGTGGGAAGGCCTTTATTCTTGGCTCTCAACTTACTTACCATCAGAGAATT
NM_016536.3       TGTAAGGATTGTGGGAAGGCCTTTATTCTTGGCTCTCAACTTACTTACCATCAGAGAATT
                  ************************************************************

HsCD00514196      CATAGTGGTGAGAAACCTTATGAGTGTAAGGAATGTGGAAAGGCCTTTATTCTTGGTTCA
NM_016536.3       CATAGTGGTGAGAAACCTTATGAGTGTAAGGAATGTGGAAAGGCCTTTATTCTTGGTTCA
                  ************************************************************

HsCD00514196      CACCTTACATACCATCAGAGAGTTCATACTGGTGAAAAGCCTTACATATGTAAAGAATGT
NM_016536.3       CACCTTACATACCATCAGAGAGTTCATACTGGTGAAAAGCCTTACATATGTAAAGAATGT
                  ************************************************************

HsCD00514196      GGGAAAGCCTTTTTATGTGCCTCCCAACTGAATGAACATCAGAGAATTCATACAGGAGAG
NM_016536.3       GGGAAAGCCTTTTTATGTGCCTCCCAACTGAATGAACATCAGAGAATTCATACAGGAGAG
                  ************************************************************

HsCD00514196      AAACCCTATGAATGTAAAGAATGCGGGAAGACCTTTTTTCGTGGCTCACAACTTACTTAC
NM_016536.3       AAACCCTATGAATGTAAAGAATGCGGGAAGACCTTTTTTCGTGGCTCACAACTTACTTAC
                  ************************************************************

HsCD00514196      CACCTGAGAGTTCATTCAGGTGAGAGACCTTATAAATGCAAAGAATGTGGGAAAGCCTTT
NM_016536.3       CACCTGAGAGTTCATTCAGGTGAGAGACCTTATAAATGCAAAGAATGTGGGAAAGCCTTT
                  ************************************************************

HsCD00514196      ATTTCTAATTCTAATCTTATTCAACATCAAAGAATTCATACCGGAGAGAAGCCCTACAAA
NM_016536.3       ATTTCTAATTCTAATCTTATTCAACATCAAAGAATTCATACCGGAGAGAAGCCCTACAAA
                  ************************************************************

HsCD00514196      TGTAAGGAATGTGGAAAGGCCTTTATTTGTGGCAAACAACTTAGTGAACATCAGAGAATT
NM_016536.3       TGTAAGGAATGTGGAAAGGCCTTTATTTGTGGCAAACAACTTAGTGAACATCAGAGAATT
                  ************************************************************

HsCD00514196      CATACAGGTGAGAAACCCTTTGAATGTAAGGAATGTGGAAAGGCCTTTATTCGTGTTGCA
NM_016536.3       CATACAGGTGAGAAACCCTTTGAATGTAAGGAATGTGGAAAGGCCTTTATTCGTGTTGCA
                  ************************************************************

HsCD00514196      TATCTTACTCAACATGAGAAAATTCATGGTGAGAAACATTATGAATGTAAGGAATGTGGG
NM_016536.3       TATCTTACTCAACATGAGAAAATTCATGGTGAGAAACATTATGAATGTAAGGAATGTGGG
                  ************************************************************

HsCD00514196      AAGACCTTTGTACGTGCTACACAACTTACATATCATCAAAGAATTCATACAGGTGAAAAG
NM_016536.3       AAGACCTTTGTACGTGCTACACAACTTACATATCATCAAAGAATTCATACAGGTGAAAAG
                  ************************************************************

HsCD00514196      CCCTACAAATGTAAGGAATGTGACAAGGCCTTTATTTATGGCTCACAACTTAGTGAACAT
NM_016536.3       CCCTACAAATGTAAGGAATGTGACAAGGCCTTTATTTATGGCTCACAACTTAGTGAACAT
                  ************************************************************

HsCD00514196      CAGAGAATTCACAGAGGTGAAAAACCTTATGAATGTAAACAGTGTGGGAAGGCTTTTATT
NM_016536.3       CAGAGAATTCACAGAGGTGAAAAACCTTATGAATGTAAACAGTGTGGGAAGGCTTTTATT
                  ************************************************************

HsCD00514196      CGTGGCTCACACCTTACTGAACATCTGAGAACTCATACTGGAGAGAAACCCTATGAATGT
NM_016536.3       CGTGGCTCACACCTTACTGAACATCTGAGAACTCATACTGGAGAGAAACCCTATGAATGT
                  ************************************************************

HsCD00514196      AAGGAATGTGGGAGGGCCTTTAGTCGTGGCTCAGAACATACTCTGCATCAAAGGATCCAT
NM_016536.3       AAGGAATGTGGGAGGGCCTTTAGTCGTGGCTCAGAACTTACTCTGCATCAAAGGATCCAT
                  *************************************:**********************

HsCD00514196      ACTGGTGAGAAACCCTATACATGTGTCCAGTGTGGTAAAGACTTTAGATGTCCTTCACAA
NM_016536.3       ACTGGTGAGAAACCCTATACATGTGTCCAGTGTGGTAAAGACTTTAGATGTCCTTCACAA
                  ************************************************************

HsCD00514196      CTTACTCAACATACAAGGCTTCATAAT---
NM_016536.3       CTTACTCAACATACAAGGCTTCATAATTGA
                  ***************************