DNASU Plasmid | ECD (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ECD

Gene Name: ecdysoneless homolog (Drosophila)

Original Clone ID:

ccsbBroadEn_02671

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC000721.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00514346

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1933nts

Open reading frame: 1 to 1933

Reference Sequence Alignment

ATGGAAGAAACCATGAAGCTTGCTACGATGGAAGACACAGTGGAGTACTGCCTGTTCCTG
ATACCAGATGAGTCAAGGGACTCAGATAAACATAAAGAGATTCTTCAGAAGTACATTGAG
AGAATAATCACTCGGTTTGCACCTATGCTGGTCCCCTACATCTGGCAGAATCAGCCTTTC
AATCTTAAATATAAACCTGGGAAAGGAGGTGTTCCTGCTCATATGTTTGGCGTGACAAAG
TTTGGGGATAACATTGAGGATGAATGGTTTATTGTTTATGTAATAAAGCAGATCACAAAG
GAATTTCCAGAGTTAGTAGCAAGGATTGAAGACAATGATGGTGAATTCTTGTTAATAGAA
GCTGCTGACTTTCTCCCTAAATGGCTGGATCCTGAAAATAGCACCAATAGGGTATTTTTC
TGCCATGGGGAATTGTGTATTATCCCTGCACCAAGAAAATCTGGAGCAGAATCTTGGTTA
CCCACCACACCCCCAACAATTCCACAAGCATTGAATATAATCACAGCACATTCAGAAAAA
ATACTTGCTTCAGAATCTATACGAGCTGCTGTGAATAGGCGCATCAGAGGGTACCCAGAA
AAAATTCAGGCCTCACTTCATCGAGCACACTGCTTCCTTCCAGCTGGCATTGTGGCAGTG
CTAAAGCAGCGCCCCAGATTGGTGGCTGCAGCAGTCCAGGCATTTTACCTACGAGACCCT
ATTGACCTGCGAGCTTGTCGTGTTTTCAAGACATTCTTGCCTGAAACACGAATAATGACA
TCGGTCACATTCACTAAATGTCTATATGCACAATTGGTGCAACAAAGGTTTGTGCCAGAC
CGGCGGAGTGGATACAGGCTGCCTCCTCCATCTGATCCCCAGTACCGAGCCCATGAATTG
GGCATGAAATTGGCTCATGGATTTGAGATCTTATGCTCCAAATGTAGCCCACATTTTTCT
GACTGCAAGAAATCCCTTGTGACTGCCTCACCACTCTGGGCCAGTTTCCTTGAAAGTCTG
AAAAAGAATGATTACTTTAAGGGACTGATAGAAGGTTCTGCTCAGTACCGGGAAAGGCTA
GAAATGGCAGAGAATTACTTCCAGCTCTCAGTAGACTGGCCAGAAAGTTCTCTTGCTATG
AGCCCTGGTGAAGAAATCTTAACCTTATTACAGACAATACCATTTGATATAGAAGACCTT
AAGAAAGAAGCAGCTAATCTTCCCCCAGAGGATGATGACCAGTGGTTAGATCTCTCACCA
GATCAGCTGGACCAGCTGCTGCAGGAAGCTGTTGGCAAAAAAGAATCCGAGTCTGTTTCC
AAGGAGGAGAAGGAGCAGAACTATGACTTAACTGAAGTCTCAGAGAGCATGAAAGCTTTC
ATATCCAAAGTCTCAACCCACAAGGGAGCAGAGCTGCCTCGAGAACCTTCTGAGGCTCCA
ATCACTTTTGATGCAGATTCTTTTCTTAATTATTTTGATAAGATTTTAGGGCCAAGGCCT
AATGAGTCAGATTCTGATGATCTGGATGATGAAGACTTTGAATGTTTAGATAGTGATGAT
GACTTGGACTTTGAAACACACGAACCTGGCGAAGAGGCTTCCCTGAAAGGAACACTTGAT
AATCTCAAGTCATACATGGCCCAGATGGACCAGGAACTAGCACACACCTGCATCAGCAAA
AGTTTCACCACTAGGAACCAAGTGGAACCTGTATCCCAGACTACCGATAACAATTCAGAT
GAGGAAGATTCTGGTACGGGAGAATCTGTTATGGCACCAGTAGATGTAGACCTGAACCTG
GTTTCAAATATATTGGAATCCTATAGCTCCCAAGCTGGACTGGCAGGACCTGCTTCCAAT
CTTTTACAAAGCATGGGAGTGCAGCTGCCTGACAACACCGATCACAGACCAACAAGTAAG
CCAACAAAAAAT

Protein sequence predicted based on nucleotide sequence

MEETMKLATMEDTVEYCLFLIPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPF
NLKYKPGKGGVPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIE
AADFLPKWLDPENSTNRVFFCHGELCIIPAPRKSGAESWLPTTPPTIPQALNIITAHSEK
ILASESIRAAVNRRIRGYPEKIQASLHRAHCFLPAGIVAVLKQRPRLVAAAVQAFYLRDP
IDLRACRVFKTFLPETRIMTSVTFTKCLYAQLVQQRFVPDRRSGYRLPPPSDPQYRAHEL
GMKLAHGFEILCSKCSPHFSDCKKSLVTASPLWASFLESLKKNDYFKGLIEGSAQYRERL
EMAENYFQLSVDWPESSLAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSP
DQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAP
ITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLD
NLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNL
VSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ECD

Symbol Nomenclature :

ECD

SYNONYM :

GCR2; HSGT1

Designation :

protein SGT1|protein ecdysoneless homolog|suppressor of GCR2|suppressor of S. cerevisiae gcr2

Full Nomenclature :

ecdysoneless homolog (Drosophila)

GENEID :

11319

GenBank Accession :

DQ895366

HGNC :

17029

Vega :

OTTHUMG00000018451

Target GenBank:

DQ895366

Reference Sequence Alignment

  

HsCD00514346        ATGGAAGAAACCATGAAGCTTGCTACGATGGAAGACACAGTGGAGTACTGCCTGTTCCTG
NM_001135752.1      ATGGAAGAAACCATGAAGCTTGCTACGATGGAAGACACAGTGGAGTACTGCCTGTTCCTG
NM_001135753.1      ATGGAAGAAACCATGAAGCTTGCTACGATGGAAGACACAGTGGAGTACTGCCTGTTCCTG
NM_007265.2         ATGGAAGAAACCATGAAGCTTGCTACGATGGAAGACACAGTGGAGTACTGCCTGTTCCTG
                    ************************************************************

HsCD00514346        ATACCAGATGAGTCAAGGGACTCAGATAAACATAAAGAGATTCTTCAGAAGTACATTGAG
NM_001135752.1      ATACCAGATGAGTCAAGGGACTCAGATAAACATAAAGAGATTCTTCAGAAGTACATTGAG
NM_001135753.1      ATACCAGATGAGTCAAGGGACTCAGATAAACATAAAGAGATTCTTCAGAAGTACATTGAG
NM_007265.2         ATACCAGATGAGTCAAGGGACTCAGATAAACATAAAGAGATTCTTCAGAAGTACATTGAG
                    ************************************************************

HsCD00514346        AGAATAATCACTCGGTTTGCACCTATGCTGGTCCCCTACATCTGGCAGAATCAGCCTTTC
NM_001135752.1      AGAATAATCACTCGGTTTGCACCTATGCTGGTCCCCTACATCTGGCAGAATCAGCCTTTC
NM_001135753.1      AGAATAATCACTCGGTTTGCACCTATGCTGGTCCCCTACATCTGGCAGAATCAGCCTTTC
NM_007265.2         AGAATAATCACTCGGTTTGCACCTATGCTGGTCCCCTACATCTGGCAGAATCAGCCTTTC
                    ************************************************************

HsCD00514346        AATCTTAAATATAAACCTGGGAAAGGAGGTGTTCCTGCTCATATGTTTGGCGTGACAAAG
NM_001135752.1      AATCTTAAATATAAACCTGGGAAAGGAGGTGTTCCTGCTCATATGTTTGGCGTGACAAAG
NM_001135753.1      AATCTTAAATATAAACCTGGGAAAGGAGGTGTTCCTGCTCATATGTTTGGCGTGACAAAG
NM_007265.2         AATCTTAAATATAAACCTGGGAAAGGAGGTGTTCCTGCTCATATGTTTGGCGTGACAAAG
                    ************************************************************

HsCD00514346        TTTGGGGATAACATTGAGGATGAATGGTTTATTGTTTATGTAATAAAGCAGATCACAAAG
NM_001135752.1      TTTGGGGATAACATTGAGGATGAATGGTTTATTGTTTATGTAATAAAGCAGATCACAAAG
NM_001135753.1      TTTGGGGATAACATTGAGGATGAATGGTTTATTGTTTATGTAATAAAGCAGATCACAAAG
NM_007265.2         TTTGGGGATAACATTGAGGATGAATGGTTTATTGTTTATGTAATAAAGCAGATCACAAAG
                    ************************************************************

HsCD00514346        GAATTTCCAGAGTTAGTAGCAAGGATTGAAGACAATGATGGTGAATTCTTGTTAATAGAA
NM_001135752.1      GAATTTCCAGAGTTAGTAGCAAGGATTGAAGACAATGATGGTGAATTCTTGTTAATAGAA
NM_001135753.1      GAATTTCCAGAGTTAGTAGCAAGGATTGAAGACAATGATGGTGAATTCTTGTTAATAGAA
NM_007265.2         GAATTTCCAGAGTTAGTAGCAAGGATTGAAGACAATGATGGTGAATTCTTGTTAATAGAA
                    ************************************************************

HsCD00514346        GCTGCTGACTTTCTCCCTAAATGGCTGGATCCTGAAAATAGCACCAATAGGGTATTTTTC
NM_001135752.1      GCTGCTGACTTTCTCCCTAAATGGCTGGATCCTGAAAATAGCACCAATAGGGTATTTTTC
NM_001135753.1      GCTGCTGACTTTCTCCCTAAATGGCTGGATCCTGAAAATAGCACCAATAGGGTATTTTTC
NM_007265.2         GCTGCTGACTTTCTCCCTAAATGGCTGGATCCTGAAAATAGCACCAATAGGGTATTTTTC
                    ************************************************************

HsCD00514346        TGCCATGGGGAATTGTGTATTATCCCTGCACCAAGAAAATCTGGAGCAGAATCTTGGTTA
NM_001135752.1      TGCCATGGGGAATTGTGTATTATCCCTGCACCAAGAAAATCTGGAGCAGAATCTTGGTTA
NM_001135753.1      TGCCATGGGGAATTGTGTATTATCCCTGCACCAAGAAAATCTGGAGCAGAATCTTGGTTA
NM_007265.2         TGCCATGGGGAATTGTGTATTATCCCTGCACCAAGAAAATCTGGAGCAGAATCTTGGTTA
                    ************************************************************

HsCD00514346        CCCACCACACCCCCAACAATTCCACAAGCATTGAATATAATCACAGCACATTCAGAAAAA
NM_001135752.1      CCCACCACACCCCCAACAATTCCACAAGCATTGAATATAATCACAGCACATTCAGAAAAA
NM_001135753.1      CCCACCACACCCCCAACAATTCCACAAGCATTGAATATAATCACAGCACATTCAGAAAAA
NM_007265.2         CCCACCACACCCCCAACAATTCCACAAGCATTGAATATAATCACAGCACATTCAGAAAAA
                    ************************************************************

HsCD00514346        ATACTTGCTTCAGAATCTATACGAGCTGCTGTGAATAGGCGCATCAGAGGGTACCCAGAA
NM_001135752.1      ATACTTGCTTCAGAATCTATACGAGCTGCTGTGAATAGGCGCATCAGAGGGTACCCAGAA
NM_001135753.1      ATACTTGCTTCAGAATCTATACGAGCTGCTGTGAATAGGCGCATCAGAGGGTACCCAGAA
NM_007265.2         ATACTTGCTTCAGAATCTATACGAGCTGCTGTGAATAGGCGCATCAGAGGGTACCCAGAA
                    ************************************************************

HsCD00514346        AAAATTCAGGCCTCACTTCATCGAGCACACTGCTTCCTTCCAGCTGGCATTGTGGCAGTG
NM_001135752.1      AAAATTCAGGCCTCACTTCATCGAGCACACTGCTTCCTTCCAGCTGGCATTGTGGCAGTG
NM_001135753.1      AAAATTCAGGCCTCACTTCATCGAGCACACTGCTTCCTTCCAGCTGGCATTGTGGCAGTG
NM_007265.2         AAAATTCAGGCCTCACTTCATCGAGCACACTGCTTCCTTCCAGCTGGCATTGTGGCAGTG
                    ************************************************************

HsCD00514346        CTAAAGCAGCGCCCCAGATTGGTGGCTGCAGCAGTCCAGGCATTTTACCTACGAGACCCT
NM_001135752.1      CTAAAGCAGCGCCCCAGATTGGTGGCTGCAGCAGTCCAGGCATTTTACCTACGAGACCCT
NM_001135753.1      CTAAAGCAGCGCCCCAGATTGGTGGCTGCAGCAGTCCAGGCATTTTACCTACGAGACCCT
NM_007265.2         CTAAAGCAGCGCCCCAGATTGGTGGCTGCAGCAGTCCAGGCATTTTACCTACGAGACCCT
                    ************************************************************

HsCD00514346        ATTGACCTGCGAGCTTGTCGTGTTTTCAAGACATTCTTGCCTGAAACACGAATAATGACA
NM_001135752.1      ATTGACCTGCGAGCTTGTCGTGTTTTCAAGACATTCTTGCCTGAAACACGAATAATGACA
NM_001135753.1      ATTGACCTGCGAGCTTGTCGTGTTTTCAAGACATTCTTGCCTGAAACACGAATAATGACA
NM_007265.2         ATTGACCTGCGAGCTTGTCGTGTTTTCAAGACATTCTTGCCTGAAACACGAATAATGACA
                    ************************************************************

HsCD00514346        TCGGTCACATTCACTAAATGTCTATATGCACAATTGGTGCAACAAAGGTTTGTGCCAGAC
NM_001135752.1      TCGGTCACATTCACTAAATGTCTATATGCACAATTGGTGCAACAAAGGTTTGTGCCAGAC
NM_001135753.1      TCG---------------------------------------------------------
NM_007265.2         TCGGTCACATTCACTAAATGTCTATATGCACAATTGGTGCAACAAAGGTTTGTGCCAGAC
                    ***                                                         

HsCD00514346        CGGCGGAGTGGATACAGGCTGCCTCCTCCATCTGATCCCCAGTACCGAGCCCATGAATTG
NM_001135752.1      CGGCGGAGTGGATACAGGCTGCCTCCTCCATCTGATCCCCAGTACCGAGCCCATGAATTG
NM_001135753.1      ------------------------------------------------------------
NM_007265.2         CGGCGGAGTGGATACAGGCTGCCTCCTCCATCTGATCCCCAGTACCGAGCCCATGAATTG
                                                                                

HsCD00514346        GGCATGAAATTGGCTCATGGATTTGAGATCTTATGCTCCAAATGTAGCCCACATTTTTCT
NM_001135752.1      GGCATGAAATTGGCTCATGGATTTGAGATCTTATGCTCCAAATGTAGCCCACATTTTTCT
NM_001135753.1      ------------GCTCATGGATTTGAGATCTTATGCTCCAAATGTAGCCCACATTTTTCT
NM_007265.2         GGCATGAAATTGGCTCATGGATTTGAGATCTTATGCTCCAAATGTAGCCCACATTTTTCT
                                ************************************************

HsCD00514346        GACTGCAAGAAATCCCTTGTGACTGCCTCACCACTCTGGGCCAGTTTCCTTGAAAGTCTG
NM_001135752.1      GACTGCAAGAAATCCCTTGTGACTGCCTCACCACTCTGGGCCAGTTTCCTTGAAAGTCTG
NM_001135753.1      GACTGCAAGAAATCCCTTGTGACTGCCTCACCACTCTGGGCCAGTTTCCTTGAAAGTCTG
NM_007265.2         GACTGCAAGAAATCCCTTGTGACTGCCTCACCACTCTGGGCCAGTTTCCTTGAAAGTCTG
                    ************************************************************

HsCD00514346        AAAAAGAATGATTACTTTAAGGGACTGATAGAAGGTTCTGCTCAGTACCGGGAAAGGCTA
NM_001135752.1      AAAAAGAATGATTACTTTAAGGGACTGATAGAAGGTTCTGCTCAGTACCGGGAAAGGCTA
NM_001135753.1      AAAAAGAATGATTACTTTAAGGGACTGATAGAAGGTTCTGCTCAGTACCGGGAAAGGCTA
NM_007265.2         AAAAAGAATGATTACTTTAAGGGACTGATAGAAGGTTCTGCTCAGTACCGGGAAAGGCTA
                    ************************************************************

HsCD00514346        GAAATGGCAGAGAATTACTTCCAGCTCTCAGTAGACTGGCCAGAAAG-------------
NM_001135752.1      GAAATGGCAGAGAATTACTTCCAGCTCTCAGTAGACTGGCCAGAAAGGTTGGAGGTGCAG
NM_001135753.1      GAAATGGCAGAGAATTACTTCCAGCTCTCAGTAGACTGGCCAGAAAG-------------
NM_007265.2         GAAATGGCAGAGAATTACTTCCAGCTCTCAGTAGACTGGCCAGAAAG-------------
                    ***********************************************             

HsCD00514346        ------------------------------------------------------------
NM_001135752.1      TGGCGCGATCCTGGCTTACTGCAAGCTCCACCTCCTGGGTTCACGCCATTCATCTGCCTC
NM_001135753.1      ------------------------------------------------------------
NM_007265.2         ------------------------------------------------------------
                                                                                

HsCD00514346        --------------------------TTCTCTTGCTATGAGCCCTGGTGAAGAAATCTTA
NM_001135752.1      AGCCTCCTGAGTACCTGGGACAACAGTTCTCTTGCTATGAGCCCTGGTGAAGAAATCTTA
NM_001135753.1      --------------------------TTCTCTTGCTATGAGCCCTGGTGAAGAAATCTTA
NM_007265.2         --------------------------TTCTCTTGCTATGAGCCCTGGTGAAGAAATCTTA
                                              **********************************

HsCD00514346        ACCTTATTACAGACAATACCATTTGATATAGAAGACCTTAAGAAAGAAGCAGCTAATCTT
NM_001135752.1      ACCTTATTACAGACAATACCATTTGATATAGAAGACCTTAAGAAAGAAGCAGCTAATCTT
NM_001135753.1      ACCTTATTACAGACAATACCATTTGATATAGAAGACCTTAAGAAAGAAGCAGCTAATCTT
NM_007265.2         ACCTTATTACAGACAATACCATTTGATATAGAAGACCTTAAGAAAGAAGCAGCTAATCTT
                    ************************************************************

HsCD00514346        CCCCCAGAGGATGATGACCAGTGGTTAGATCTCTCACCAGATCAGCTGGACCAGCTGCTG
NM_001135752.1      CCCCCAGAGGATGATGACCAGTGGTTAGATCTCTCACCAGATCAGCTGGACCAGCTGCTG
NM_001135753.1      CCCCCAGAGGATGATGACCAGTGGTTAGATCTCTCACCAGATCAGCTGGACCAGCTGCTG
NM_007265.2         CCCCCAGAGGATGATGACCAGTGGTTAGATCTCTCACCAGATCAGCTGGACCAGCTGCTG
                    ************************************************************

HsCD00514346        CAGGAAGCTGTTGGCAAAAAAGAATCCGAGTCTGTTTCCAAGGAGGAGAAGGAGCAGAAC
NM_001135752.1      CAGGAAGCTGTTGGCAAAAAAGAATCCGAGTCTGTTTCCAAGGAGGAGAAGGAGCAGAAC
NM_001135753.1      CAGGAAGCTGTTGGCAAAAAAGAATCCGAGTCTGTTTCCAAGGAGGAGAAGGAGCAGAAC
NM_007265.2         CAGGAAGCTGTTGGCAAAAAAGAATCCGAGTCTGTTTCCAAGGAGGAGAAGGAGCAGAAC
                    ************************************************************

HsCD00514346        TATGACTTAACTGAAGTCTCAGAGAGCATGAAAGCTTTCATATCCAAAGTCTCAACCCAC
NM_001135752.1      TATGACTTAACTGAAGTCTCAGAGAGCATGAAAGCTTTCATATCCAAAGTCTCAACCCAC
NM_001135753.1      TATGACTTAACTGAAGTCTCAGAGAGCATGAAAGCTTTCATATCCAAAGTCTCAACCCAC
NM_007265.2         TATGACTTAACTGAAGTCTCAGAGAGCATGAAAGCTTTCATATCCAAAGTCTCAACCCAC
                    ************************************************************

HsCD00514346        AAGGGAGCAGAGCTGCCTCGAGAACCTTCTGAGGCTCCAATCACTTTTGATGCAGATTCT
NM_001135752.1      AAGGGAGCAGAGCTGCCTCGAGAACCTTCTGAGGCTCCAATCACTTTTGATGCAGATTCT
NM_001135753.1      AAGGGAGCAGAGCTGCCTCGAGAACCTTCTGAGGCTCCAATCACTTTTGATGCAGATTCT
NM_007265.2         AAGGGAGCAGAGCTGCCTCGAGAACCTTCTGAGGCTCCAATCACTTTTGATGCAGATTCT
                    ************************************************************

HsCD00514346        TTTCTTAATTATTTTGATAAGATTTTAGGGCCAAGGCCTAATGAGTCAGATTCTGATGAT
NM_001135752.1      TTTCTTAATTATTTTGATAAGATTTTAGGGCCAAGGCCTAATGAGTCAGATTCTGATGAT
NM_001135753.1      TTTCTTAATTATTTTGATAAGATTTTAGGGCCAAGGCCTAATGAGTCAGATTCTGATGAT
NM_007265.2         TTTCTTAATTATTTTGATAAGATTTTAGGGCCAAGGCCTAATGAGTCAGATTCTGATGAT
                    ************************************************************

HsCD00514346        CTGGATGATGAAGACTTTGAATGTTTAGATAGTGATGATGACTTGGACTTTGAAACACAC
NM_001135752.1      CTGGATGATGAAGACTTTGAATGTTTAGATAGTGATGATGACTTGGACTTTGAAACACAC
NM_001135753.1      CTGGATGATGAAGACTTTGAATGTTTAGATAGTGATGATGACTTGGACTTTGAAACACAC
NM_007265.2         CTGGATGATGAAGACTTTGAATGTTTAGATAGTGATGATGACTTGGACTTTGAAACACAC
                    ************************************************************

HsCD00514346        GAACCTGGCGAAGAGGCTTCCCTGAAAGGAACACTTGATAATCTCAAGTCATACATGGCC
NM_001135752.1      GAACCTGGCGAAGAGGCTTCCCTGAAAGGAACACTTGATAATCTCAAGTCATACATGGCC
NM_001135753.1      GAACCTGGCGAAGAGGCTTCCCTGAAAGGAACACTTGATAATCTCAAGTCATACATGGCC
NM_007265.2         GAACCTGGCGAAGAGGCTTCCCTGAAAGGAACACTTGATAATCTCAAGTCATACATGGCC
                    ************************************************************

HsCD00514346        CAGATGGACCAGGAACTAGCACACACCTGCATCAGCAAAAGTTTCACCACTAGGAACCAA
NM_001135752.1      CAGATGGACCAGGAACTAGCACACACCTGCATCAGCAAAAGTTTCACCACTAGGAACCAA
NM_001135753.1      CAGATGGACCAGGAACTAGCACACACCTGCATCAGCAAAAGTTTCACCACTAGGAACCAA
NM_007265.2         CAGATGGACCAGGAACTAGCACACACCTGCATCAGCAAAAGTTTCACCACTAGGAACCAA
                    ************************************************************

HsCD00514346        GTGGAACCTGTATCCCAGACTACCGATAACAATTCAGATGAGGAAGATTCTGGTACGGGA
NM_001135752.1      GTGGAACCTGTATCCCAGACTACCGATAACAATTCAGATGAGGAAGATTCTGGTACGGGA
NM_001135753.1      GTGGAACCTGTATCCCAGACTACCGATAACAATTCAGATGAGGAAGATTCTGGTACGGGA
NM_007265.2         GTGGAACCTGTATCCCAGACTACCGATAACAATTCAGATGAGGAAGATTCTGGTACGGGA
                    ************************************************************

HsCD00514346        GAATCTGTTATGGCACCAGTAGATGTAGACCTGAACCTGGTTTCAAATATATTGGAATCC
NM_001135752.1      GAATCTGTTATGGCACCAGTAGATGTAGACCTGAACCTGGTTTCAAATATATTGGAATCC
NM_001135753.1      GAATCTGTTATGGCACCAGTAGATGTAGACCTGAACCTGGTTTCAAATATATTGGAATCC
NM_007265.2         GAATCTGTTATGGCACCAGTAGATGTAGACCTGAACCTGGTTTCAAATATATTGGAATCC
                    ************************************************************

HsCD00514346        TATAGCTCCCAAGCTGGACTGGCAGGACCTGCTTCCAATCTTTTACAAAGCATGGGAGTG
NM_001135752.1      TATAGCTCCCAAGCTGGACTGGCAGGACCTGCTTCCAATCTTTTACAAAGCATGGGAGTG
NM_001135753.1      TATAGCTCCCAAGCTGGACTGGCAGGACCTGCTTCCAATCTTTTACAAAGCATGGGAGTG
NM_007265.2         TATAGCTCCCAAGCTGGACTGGCAGGACCTGCTTCCAATCTTTTACAAAGCATGGGAGTG
                    ************************************************************

HsCD00514346        CAGCTGCCTGACAACACCGATCACAGACCAACAAGTAAGCCAACAAAAAAT---
NM_001135752.1      CAGCTGCCTGACAACACCGATCACAGACCAACAAGTAAGCCAACAAAAAATTAA
NM_001135753.1      CAGCTGCCTGACAACACCGATCACAGACCAACAAGTAAGCCAACAAAAAATTAA
NM_007265.2         CAGCTGCCTGACAACACCGATCACAGACCAACAAGTAAGCCAACAAAAAATTAA
                    ***************************************************