DNASU Plasmid | MRPS12 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: MRPS12

Gene Name: mitochondrial ribosomal protein S12

Original Clone ID:

ccsbBroadEn_01444

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC001617.1,BC007499.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00514752

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 415nts

Open reading frame: 1 to 415

Reference Sequence Alignment

ATGTCCTGGTCTGGCCTTCTCCATGGCCTCAACACGTCCCTAACTTGTGGCCCAGCTCTG
GTTCCCCGGCTCTGGGCTACCTGCTCCATGGCTACCCTGAACCAGATGCACCGCCTGGGG
CCCCCCAAGCGGCCGCCTCGGAAGCTGGGCCCCACGGAAGGCCGGCCGCAGCTGAAGGGT
GTGGTCCTGTGCACGTTTACCCGCAAGCCGAAGAAGCCCAACTCAGCCAATCGCAAGTGC
TGTCGAGTGCGGCTCAGCACTGGCCGCGAGGCCGTCTGCTTCATCCCTGGGGAGGGCCAC
ACCCTGCAGGAGCACCAGATTGTCCTTGTGGAGGGCGGCCGCACCCAGGACCTGCCAGGC
GTCAAGCTCACCGTTGTGCGTGGCAAGTACGACTGTGGCCACGTGCAGAAGAAG

Protein sequence predicted based on nucleotide sequence

MSWSGLLHGLNTSLTCGPALVPRLWATCSMATLNQMHRLGPPKRPPRKLGPTEGRPQLKG
VVLCTFTRKPKKPNSANRKCCRVRLSTGREAVCFIPGEGHTLQEHQIVLVEGGRTQDLPG
VKLTVVRGKYDCGHVQKK

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

MRPS12

Symbol Nomenclature :

MRPS12

SYNONYM :

MPR-S12; MT-RPS12; RPMS12; RPS12; RPSM12

Designation :

28S ribosomal protein S12, mitochondrial|MRP-S12|S12mt

Full Nomenclature :

mitochondrial ribosomal protein S12

GENEID :

6183

HGNC :

10380

MIM :

603021

Vega :

OTTHUMG00000182692

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00514752      ATGTCCTGGTCTGGCCTTCTCCATGGCCTCAACACGTCCCTAACTTGTGGCCCAGCTCTG
NM_021107.1       ATGTCCTGGTCTGGCCTTCTCCATGGCCTCAACACGTCCCTAACTTGTGGCCCAGCTCTG
NM_033362.3       ATGTCCTGGTCTGGCCTTCTCCATGGCCTCAACACGTCCCTAACTTGTGGCCCAGCTCTG
NM_033363.1       ATGTCCTGGTCTGGCCTTCTCCATGGCCTCAACACGTCCCTAACTTGTGGCCCAGCTCTG
                  ************************************************************

HsCD00514752      GTTCCCCGGCTCTGGGCTACCTGCTCCATGGCTACCCTGAACCAGATGCACCGCCTGGGG
NM_021107.1       GTTCCCCGGCTCTGGGCTACCTGCTCCATGGCTACCCTGAACCAGATGCACCGCCTGGGG
NM_033362.3       GTTCCCCGGCTCTGGGCTACCTGCTCCATGGCTACCCTGAACCAGATGCACCGCCTGGGG
NM_033363.1       GTTCCCCGGCTCTGGGCTACCTGCTCCATGGCTACCCTGAACCAGATGCACCGCCTGGGG
                  ************************************************************

HsCD00514752      CCCCCCAAGCGGCCGCCTCGGAAGCTGGGCCCCACGGAAGGCCGGCCGCAGCTGAAGGGT
NM_021107.1       CCCCCCAAGCGGCCGCCTCGGAAGCTGGGCCCCACGGAAGGCCGGCCGCAGCTGAAGGGT
NM_033362.3       CCCCCCAAGCGGCCGCCTCGGAAGCTGGGCCCCACGGAAGGCCGGCCGCAGCTGAAGGGT
NM_033363.1       CCCCCCAAGCGGCCGCCTCGGAAGCTGGGCCCCACGGAAGGCCGGCCGCAGCTGAAGGGT
                  ************************************************************

HsCD00514752      GTGGTCCTGTGCACGTTTACCCGCAAGCCGAAGAAGCCCAACTCAGCCAATCGCAAGTGC
NM_021107.1       GTGGTCCTGTGCACGTTTACCCGCAAGCCGAAGAAGCCCAACTCAGCCAATCGCAAGTGC
NM_033362.3       GTGGTCCTGTGCACGTTTACCCGCAAGCCGAAGAAGCCCAACTCAGCCAATCGCAAGTGC
NM_033363.1       GTGGTCCTGTGCACGTTTACCCGCAAGCCGAAGAAGCCCAACTCAGCCAATCGCAAGTGC
                  ************************************************************

HsCD00514752      TGTCGAGTGCGGCTCAGCACTGGCCGCGAGGCCGTCTGCTTCATCCCTGGGGAGGGCCAC
NM_021107.1       TGTCGAGTGCGGCTCAGCACTGGCCGCGAGGCCGTCTGCTTCATCCCTGGGGAGGGCCAC
NM_033362.3       TGTCGAGTGCGGCTCAGCACTGGCCGCGAGGCCGTCTGCTTCATCCCTGGGGAGGGCCAC
NM_033363.1       TGTCGAGTGCGGCTCAGCACTGGCCGCGAGGCCGTCTGCTTCATCCCTGGGGAGGGCCAC
                  ************************************************************

HsCD00514752      ACCCTGCAGGAGCACCAGATTGTCCTTGTGGAGGGCGGCCGCACCCAGGACCTGCCAGGC
NM_021107.1       ACCCTGCAGGAGCACCAGATTGTCCTTGTGGAGGGCGGCCGCACCCAGGACCTGCCAGGC
NM_033362.3       ACCCTGCAGGAGCACCAGATTGTCCTTGTGGAGGGCGGCCGCACCCAGGACCTGCCAGGC
NM_033363.1       ACCCTGCAGGAGCACCAGATTGTCCTTGTGGAGGGCGGCCGCACCCAGGACCTGCCAGGC
                  ************************************************************

HsCD00514752      GTCAAGCTCACCGTTGTGCGTGGCAAGTACGACTGTGGCCACGTGCAGAAGAAG---
NM_021107.1       GTCAAGCTCACCGTTGTGCGTGGCAAGTACGACTGTGGCCACGTGCAGAAGAAGTGA
NM_033362.3       GTCAAGCTCACCGTTGTGCGTGGCAAGTACGACTGTGGCCACGTGCAGAAGAAGTGA
NM_033363.1       GTCAAGCTCACCGTTGTGCGTGGCAAGTACGACTGTGGCCACGTGCAGAAGAAGTGA
                  ******************************************************