DNASU Plasmid | C14orf153 (H. sapiens ) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: APOPT1

Gene Name: apoptogenic 1, mitochondrial

Original Clone ID:

ccsbBroadEn_12814

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC007412.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00514920

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 580nts

Open reading frame: 1 to 580

Reference Sequence Alignment

ATGGTGGTCTTGCGGGCGGGGAAGAAGACCTTTCTCCCCGCTCTCTGCCGCGCCTTCGCC
TGCCGCGGCTGTCAACTCGCTCCGGAGCGCGGCGCCGAGCGCAGGGATACGGCGCCCAGC
GGGGTCTCAAGATTCTGCCCTCCAAGAAAGTCTTGCCATGATTGGATAGGACCCCCAGAT
AAATATTCAAACCTTCGACCTGTTCACTTTTACATACCTGAAAATGAATCTCCATTGGAA
CAAAAGCTTAGAAAATTAAGACAAGAAACACAAGAATGGAATCAACAGTTCTGGGCAAAC
CAGAATTTGACTTTTAGTAAGGAAAAAGAAGAATTTATTCACTCAAGACTAAAAACTAAA
GGCCTGGGCCTGAGAACTGAATCAGGTCAGAAAGCAACATTGAATGCAGAAGAAATGGCG
GACTTCTACAAGGAATTTTTAAGTAAAAATTTTCAGAAGCACATGTATTATAACAGAGAT
TGGTACAAGCGCAATTTTGCCATCACCTTCTTCATGGGAAAAGTGGCCCTGGAAAGGATT
TGGAACAAGCTTAAACAGAAACAAAAGAAGAGGAGCAAC

Protein sequence predicted based on nucleotide sequence

MVVLRAGKKTFLPALCRAFACRGCQLAPERGAERRDTAPSGVSRFCPPRKSCHDWIGPPD
KYSNLRPVHFYIPENESPLEQKLRKLRQETQEWNQQFWANQNLTFSKEKEEFIHSRLKTK
GLGLRTESGQKATLNAEEMADFYKEFLSKNFQKHMYYNRDWYKRNFAITFFMGKVALERI
WNKLKQKQKKRSN

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

APOPT1

Symbol Nomenclature :

APOPT1

SYNONYM :

APOP1; C14orf153

Designation :

UPF0671 protein C14orf153|apoptogenic protein 1, mitochondrial

Full Nomenclature :

apoptogenic 1, mitochondrial

GENEID :

84334

HGNC :

20492

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00514920      ---------------------------------------ATGGTGGTCTTGCGGGCGGGG
NM_032374.3       ATGCTGCCGTGCGCCGCGGGAGCCAGGGGGCGTGGGGCCATGGTGGTCTTGCGGGCGGGG
                                                         *********************

HsCD00514920      AAGAAGACCTTTCTCCCCGCTCTCTGCCGCGCCTTCGCCTGCCGCGGCTGTCAACTCGCT
NM_032374.3       AAGAAGACCTTTCTCCCCCCTCTCTGCCGCGCCTTCGCCTGCCGCGGCTGTCAACTCGCT
                  ****************** *****************************************

HsCD00514920      CCGGAGCGCGGCGCCGAGCGCAGGGATACGGCGCCCAGCGGGGTCTCAAGATTCTGCCCT
NM_032374.3       CCGGAGCGCGGCGCCGAGCGCAGGGATACGGCGCCCAGCGGGGTCTCAAGATTCTGCCCT
                  ************************************************************

HsCD00514920      CCAAGAAAGTCTTGCCATGATTGGATAGGACCCCCAGATAAATATTCAAACCTTCGACCT
NM_032374.3       CCAAGAAAGTCTTGCCATGATTGGATAGGACCCCCAGATAAATATTCAAACCTTCGACCT
                  ************************************************************

HsCD00514920      GTTCACTTTTACATACCTGAAAATGAATCTCCATTGGAACAAAAGCTTAGAAAATTAAGA
NM_032374.3       GTTCACTTTTACATACCTGAAAATGAATCTCCATTGGAACAAAAGCTTAGAAAATTAAGA
                  ************************************************************

HsCD00514920      CAAGAAACACAAGAATGGAATCAACAGTTCTGGGCAAACCAGAATTTGACTTTTAGTAAG
NM_032374.3       CAAGAAACACAAGAATGGAATCAACAGTTCTGGGCAAACCAGAATTTGACTTTTAGTAAG
                  ************************************************************

HsCD00514920      GAAAAAGAAGAATTTATTCACTCAAGACTAAAAACTAAAGGCCTGGGCCTGAGAACTGAA
NM_032374.3       GAAAAAGAAGAATTTATTCACTCAAGACTAAAAACTAAAGGCCTGGGCCTGAGAACTGAA
                  ************************************************************

HsCD00514920      TCAGGTCAGAAAGCAACATTGAATGCAGAAGAAATGGCGGACTTCTACAAGGAATTTTTA
NM_032374.3       TCAGGTCAGAAAGCAACATTGAATGCAGAAGAAATGGCGGACTTCTACAAGGAATTTTTA
                  ************************************************************

HsCD00514920      AGTAAAAATTTTCAGAAGCACATGTATTATAACAGAGATTGGTACAAGCGCAATTTTGCC
NM_032374.3       AGTAAAAATTTTCAGAAGCACATGTATTATAACAGAGATTGGTACAAGCGCAATTTTGCC
                  ************************************************************

HsCD00514920      ATCACCTTCTTCATGGGAAAAGTGGCCCTGGAAAGGATTTGGAACAAGCTTAAACAGAAA
NM_032374.3       ATCACCTTCTTCATGGGAAAAGTGGCCCTGGAAAGGATTTGGAACAAGCTTAAACAGAAA
                  ************************************************************

HsCD00514920      CAAAAGAAGAGGAGCAAC---
NM_032374.3       CAAAAGAAGAGGAGCAACTAG
                  ******************