DNASU Plasmid | F2RL2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: F2RL2

Gene Name: coagulation factor II (thrombin) receptor-like 2

Original Clone ID:

ccsbBroadEn_00528

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC093648.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515570

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1123nts

Open reading frame: 1 to 1123

Reference Sequence Alignment

ATGAAAGCCCTCATCTTTGCAGCTGCTGGCCTCCTGCTTCTGTTGCCCACTTTTTGTCAG
AGTGGCATGGAAAATGATACAAACAACTTGGCAAAGCCAACCTTACCCATTAAGACCTTT
CGTGGAGCTCCCCCAAATTCTTTTGAAGAGTTCCCCTTTTCTGCCTTGGAAGGCTGGACA
GGAGCCACGATTACTGTAAAAATTAAGTGCCCTGAAGAAAGTGCTTCACATCTCCATGTG
AAAAATGCTACCATGGGGTACCTGACCAGCTCCTTAAGTACTAAACTGATACCTGCCATC
TACCTCCTGGTGTTTGTAGTTGGTGTCCCGGCCAATGCTGTGACCCTGTGGATGCTTTTC
TTCAGGACCAGATCCATCTGTACCACTGTATTCTACACCAACCTGGCCATTGCAGATTTT
CTTTTTTGTGTTACATTGCCCTTTAAGATAGCTTATCATCTCAATGGGAACAACTGGGTA
TTTGGAGAGGTCCTGTGCCGGGCCACCACAGTCATCTTCTATGGCAACATGTACTGCTCC
ATTCTGCTCCTTGCCTGCATCAGCATCAACCGCTACCTGGCCATCGTCCATCCTTTCACC
TACCGGGGCCTGCCCAAGCACACCTATGCCTTGGTAACATGTGGACTGGTGTGGGCAACA
GTTTTCTTATATATGCTGCCATTTTTCATACTGAAGCAGGAATATTATCTTGTTCAGCCA
GACATCACCACCTGCCATGATGTTCACAACACTTGCGAGTCCTCATCTCCCTTCCAACTC
TATTACTTCATCTCCTTGGCATTCTTTGGATTCTTAATTCCATTTGTGCTTATCATCTAC
TGCTATGCAGCCATCATCCGGACACTTAATGCATACGATCATAGATGGTTGTGGTATGTT
AAGGCGAGTCTCCTCATCCTTGTGATTTTTACCATTTGCTTTGCTCCAAGCAATATTATT
CTTATTATTCACCATGCTAACTACTACTACAACAACACTGATGGCTTATATTTTATATAT
CTCATAGCTTTGTGCCTGGGTAGTCTTAATAGTTGCTTAGATCCATTCCTTTATTTTCTC
ATGTCAAAAACCAGAAATCACTCCACTGCTTACCTTACAAAA

Protein sequence predicted based on nucleotide sequence

MKALIFAAAGLLLLLPTFCQSGMENDTNNLAKPTLPIKTFRGAPPNSFEEFPFSALEGWT
GATITVKIKCPEESASHLHVKNATMGYLTSSLSTKLIPAIYLLVFVVGVPANAVTLWMLF
FRTRSICTTVFYTNLAIADFLFCVTLPFKIAYHLNGNNWVFGEVLCRATTVIFYGNMYCS
ILLLACISINRYLAIVHPFTYRGLPKHTYALVTCGLVWATVFLYMLPFFILKQEYYLVQP
DITTCHDVHNTCESSSPFQLYYFISLAFFGFLIPFVLIIYCYAAIIRTLNAYDHRWLWYV
KASLLILVIFTICFAPSNIILIIHHANYYYNNTDGLYFIYLIALCLGSLNSCLDPFLYFL
MSKTRNHSTAYLTK

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

F2RL2

Symbol Nomenclature :

F2RL2

SYNONYM :

PAR-3; PAR3

Designation :

Coagulation factor II receptor-like 2 (protease-actovated receptor 3)|protease-activated receptor 3|proteinase-activated receptor 3|proteinase-activated receptor-3|thrombin receptor-like 2

Full Nomenclature :

coagulation factor II (thrombin) receptor-like 2

GENEID :

2151

HGNC :

3539

MIM :

601919

Vega :

OTTHUMG00000102119

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515570        ATGAAAGCCCTCATCTTTGCAGCTGCTGGCCTCCTGCTTCTGTTGCCCACTTTTTGTCAG
NM_004101.3         ATGAAAGCCCTCATCTTTGCAGCTGCTGGCCTCCTGCTTCTGTTGCCCACTTTTTGTCAG
NM_001256566.1      ------------------------------------------------------------
                                                                                

HsCD00515570        AGTGGCATGGAAAATGATACAAACAACTTGGCAAAGCCAACCTTACCCATTAAGACCTTT
NM_004101.3         AGTGGCATGGAAAATGATACAAACAACTTGGCAAAGCCAACCTTACCCATTAAGACCTTT
NM_001256566.1      ------ATGGAAAATGATACAAACAACTTGGCAAAGCCAACCTTACCCATTAAGACCTTT
                          ******************************************************

HsCD00515570        CGTGGAGCTCCCCCAAATTCTTTTGAAGAGTTCCCCTTTTCTGCCTTGGAAGGCTGGACA
NM_004101.3         CGTGGAGCTCCCCCAAATTCTTTTGAAGAGTTCCCCTTTTCTGCCTTGGAAGGCTGGACA
NM_001256566.1      CGTGGAGCTCCCCCAAATTCTTTTGAAGAGTTCCCCTTTTCTGCCTTGGAAGGCTGGACA
                    ************************************************************

HsCD00515570        GGAGCCACGATTACTGTAAAAATTAAGTGCCCTGAAGAAAGTGCTTCACATCTCCATGTG
NM_004101.3         GGAGCCACGATTACTGTAAAAATTAAGTGCCCTGAAGAAAGTGCTTCACATCTCCATGTG
NM_001256566.1      GGAGCCACGATTACTGTAAAAATTAAGTGCCCTGAAGAAAGTGCTTCACATCTCCATGTG
                    ************************************************************

HsCD00515570        AAAAATGCTACCATGGGGTACCTGACCAGCTCCTTAAGTACTAAACTGATACCTGCCATC
NM_004101.3         AAAAATGCTACCATGGGGTACCTGACCAGCTCCTTAAGTACTAAACTGATACCTGCCATC
NM_001256566.1      AAAAATGCTACCATGGGGTACCTGACCAGCTCCTTAAGTACTAAACTGATACCTGCCATC
                    ************************************************************

HsCD00515570        TACCTCCTGGTGTTTGTAGTTGGTGTCCCGGCCAATGCTGTGACCCTGTGGATGCTTTTC
NM_004101.3         TACCTCCTGGTGTTTGTAGTTGGTGTCCCGGCCAATGCTGTGACCCTGTGGATGCTTTTC
NM_001256566.1      TACCTCCTGGTGTTTGTAGTTGGTGTCCCGGCCAATGCTGTGACCCTGTGGATGCTTTTC
                    ************************************************************

HsCD00515570        TTCAGGACCAGATCCATCTGTACCACTGTATTCTACACCAACCTGGCCATTGCAGATTTT
NM_004101.3         TTCAGGACCAGATCCATCTGTACCACTGTATTCTACACCAACCTGGCCATTGCAGATTTT
NM_001256566.1      TTCAGGACCAGATCCATCTGTACCACTGTATTCTACACCAACCTGGCCATTGCAGATTTT
                    ************************************************************

HsCD00515570        CTTTTTTGTGTTACATTGCCCTTTAAGATAGCTTATCATCTCAATGGGAACAACTGGGTA
NM_004101.3         CTTTTTTGTGTTACATTGCCCTTTAAGATAGCTTATCATCTCAATGGGAACAACTGGGTA
NM_001256566.1      CTTTTTTGTGTTACATTGCCCTTTAAGATAGCTTATCATCTCAATGGGAACAACTGGGTA
                    ************************************************************

HsCD00515570        TTTGGAGAGGTCCTGTGCCGGGCCACCACAGTCATCTTCTATGGCAACATGTACTGCTCC
NM_004101.3         TTTGGAGAGGTCCTGTGCCGGGCCACCACAGTCATCTTCTATGGCAACATGTACTGCTCC
NM_001256566.1      TTTGGAGAGGTCCTGTGCCGGGCCACCACAGTCATCTTCTATGGCAACATGTACTGCTCC
                    ************************************************************

HsCD00515570        ATTCTGCTCCTTGCCTGCATCAGCATCAACCGCTACCTGGCCATCGTCCATCCTTTCACC
NM_004101.3         ATTCTGCTCCTTGCCTGCATCAGCATCAACCGCTACCTGGCCATCGTCCATCCTTTCACC
NM_001256566.1      ATTCTGCTCCTTGCCTGCATCAGCATCAACCGCTACCTGGCCATCGTCCATCCTTTCACC
                    ************************************************************

HsCD00515570        TACCGGGGCCTGCCCAAGCACACCTATGCCTTGGTAACATGTGGACTGGTGTGGGCAACA
NM_004101.3         TACCGGGGCCTGCCCAAGCACACCTATGCCTTGGTAACATGTGGACTGGTGTGGGCAACA
NM_001256566.1      TACCGGGGCCTGCCCAAGCACACCTATGCCTTGGTAACATGTGGACTGGTGTGGGCAACA
                    ************************************************************

HsCD00515570        GTTTTCTTATATATGCTGCCATTTTTCATACTGAAGCAGGAATATTATCTTGTTCAGCCA
NM_004101.3         GTTTTCTTATATATGCTGCCATTTTTCATACTGAAGCAGGAATATTATCTTGTTCAGCCA
NM_001256566.1      GTTTTCTTATATATGCTGCCATTTTTCATACTGAAGCAGGAATATTATCTTGTTCAGCCA
                    ************************************************************

HsCD00515570        GACATCACCACCTGCCATGATGTTCACAACACTTGCGAGTCCTCATCTCCCTTCCAACTC
NM_004101.3         GACATCACCACCTGCCATGATGTTCACAACACTTGCGAGTCCTCATCTCCCTTCCAACTC
NM_001256566.1      GACATCACCACCTGCCATGATGTTCACAACACTTGCGAGTCCTCATCTCCCTTCCAACTC
                    ************************************************************

HsCD00515570        TATTACTTCATCTCCTTGGCATTCTTTGGATTCTTAATTCCATTTGTGCTTATCATCTAC
NM_004101.3         TATTACTTCATCTCCTTGGCATTCTTTGGATTCTTAATTCCATTTGTGCTTATCATCTAC
NM_001256566.1      TATTACTTCATCTCCTTGGCATTCTTTGGATTCTTAATTCCATTTGTGCTTATCATCTAC
                    ************************************************************

HsCD00515570        TGCTATGCAGCCATCATCCGGACACTTAATGCATACGATCATAGATGGTTGTGGTATGTT
NM_004101.3         TGCTATGCAGCCATCATCCGGACACTTAATGCATACGATCATAGATGGTTGTGGTATGTT
NM_001256566.1      TGCTATGCAGCCATCATCCGGACACTTAATGCATACGATCATAGATGGTTGTGGTATGTT
                    ************************************************************

HsCD00515570        AAGGCGAGTCTCCTCATCCTTGTGATTTTTACCATTTGCTTTGCTCCAAGCAATATTATT
NM_004101.3         AAGGCGAGTCTCCTCATCCTTGTGATTTTTACCATTTGCTTTGCTCCAAGCAATATTATT
NM_001256566.1      AAGGCGAGTCTCCTCATCCTTGTGATTTTTACCATTTGCTTTGCTCCAAGCAATATTATT
                    ************************************************************

HsCD00515570        CTTATTATTCACCATGCTAACTACTACTACAACAACACTGATGGCTTATATTTTATATAT
NM_004101.3         CTTATTATTCACCATGCTAACTACTACTACAACAACACTGATGGCTTATATTTTATATAT
NM_001256566.1      CTTATTATTCACCATGCTAACTACTACTACAACAACACTGATGGCTTATATTTTATATAT
                    ************************************************************

HsCD00515570        CTCATAGCTTTGTGCCTGGGTAGTCTTAATAGTTGCTTAGATCCATTCCTTTATTTTCTC
NM_004101.3         CTCATAGCTTTGTGCCTGGGTAGTCTTAATAGTTGCTTAGATCCATTCCTTTATTTTCTC
NM_001256566.1      CTCATAGCTTTGTGCCTGGGTAGTCTTAATAGTTGCTTAGATCCATTCCTTTATTTTCTC
                    ************************************************************

HsCD00515570        ATGTCAAAAACCAGAAATCACTCCACTGCTTACCTTACAAAA---
NM_004101.3         ATGTCAAAAACCAGAAATCACTCCACTGCTTACCTTACAAAATAG
NM_001256566.1      ATGTCAAAAACCAGAAATCACTCCACTGCTTACCTTACAAAATAG
                    ******************************************

Pathways

NCI :

CDC42 signaling events

NCI :

ErbB1 downstream signaling

NCI :

Insulin Pathway

NCI :

Nephrin/Neph1 signaling in the kidney podocyte

NCI :

PAR1-mediated thrombin signaling events

NCI :

PAR4-mediated thrombin signaling events

NCI :

RhoA signaling pathway

NCI :

Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

G alpha (q) signalling events

Reactome :

Gastrin-CREB signalling pathway via PKC and MAPK

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

Hemostasis

Reactome :

Peptide ligand-binding receptors

Reactome :

Platelet activation, signaling and aggregation

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

Reactome :

Thrombin signalling through proteinase activated receptors (PARs)

WikiPathway :

GPCRs, Class A Rhodopsin-like

Protein

HPRD :

03561

Clone

Cloning Information :

608500

Original Clone ID :

ccsbBroadEn_00528

Organism

Species Specific ID:

2151

Genome

Chromosome :

Map Location :

5q13

Ensembl :

ENSG00000164220

Reagents

Labome :

F2RL2-antibody