DNASU Plasmid | PNPLA5 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: PNPLA5

Gene Name: patatin-like phospholipase domain containing 5

Original Clone ID:

ccsbBroadEn_05041

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC031820.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515671

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1288nts

Open reading frame: 1 to 1288

Reference Sequence Alignment

ATGGGCTTCTTAGAGGAGGAGGGCAGATGGAACCTGTCCTTCTCCGGCGCCGGCTACCTG
GGCGCCCACCACGTGGGCGCCACCGAATGCCTGCGCCAGCGAGCCCCGCGCCTCCTCCAG
GGCGCCCGCCGCATCTACGGTTCCTCGTCTGGGGCGCTCAACGCAGTCAGCATCGTCTGC
GGCAAGTCGGTCGACTTCTGCTGCTCCCACCTCCTGGGCATGGTTGGGCAGTTGGAGCGG
CTGAGCCTAAGCATCCTGCACCCGGCCTACGCGCCCATCGAGCACGTCAAGCAGCAGCTG
CAGGATGCTCTGCCCCCCGACGCCCACGTCCTGGCCTCCCAGCGGCTGGGCATTTCGCTG
ACCCGCTGGCCTGACGGACGCAACTTCTTGGTCACTGACTTCGCCACCTGCGATGAGCTC
ATCCAGGCCTTGGTCTGCACCTTATACTTTCCTTTCTACTGCGGGCTGATCCCCCCCGAG
TTCAGAGGGGAGCGCTACATCGATGGGGCTCTGAGCAACAACTTGCCCTTTGCAGACTGC
CCCTCCACCATCACGGTGTCGCCCTTCCATGGGACAGTGGACATCTGCCCCCAGAGCACC
TCCCCCAACCTGCATGAGCTGAACGTCTTCAACTTCAGCTTCCAAATCTCCACTGAGAAC
TTCTTCCTGGGGCTCATATGTCTCATACCCCCCAGCCTCGAGGTAGTGGCCGACAACTGC
AGACAAGGCTACCTGGATGCCCTGAGGTTCCTGGAGAGACGTGGACTCACCAAGGAACCA
GTGCTATGGACGCTGGTGTCTAAGGAACCCCCAGCCCCGGCTGACGGAAACTGGGATGCT
GGCTGTGACCAACGCTGGAAGGGGGGCCTGTCTCTCAACTGGAAAGTGCCCCATGTGCAA
GTCAAGGATGTACCCAACTTTGAGCAGCTCTCACCAGAGCTGGAGGCTGCACTGAAGAAA
GCATGTACGAGGGATCCCAGCCGGTGGGCCCGCTTCTGGCACTCGGGGCCTGGACAGGTG
CTGACGTACCTGCTGCTACCCTGCACACTGCCCTTCGAGTACATCTACTTCCGCAGCAGA
AGGTTGGTGGTGTGGCTGCCCGATGTGCCGGCGGACTTGTGGTGGATGCAGGGCCTGCTG
AGGAACATGGCCCTCGAGGTTTTCTCCAGGACCAAGGCCCAGCTCCTTGGGCCCATCAGC
CCTCCGGCCACTCGCGTCCTGGAAACAAGCCCCCTCCAACCCCAGATAGCTCCTCATAGA
GAGGAGCTCGGGCCCACCCACCAGGCC

Protein sequence predicted based on nucleotide sequence

MGFLEEEGRWNLSFSGAGYLGAHHVGATECLRQRAPRLLQGARRIYGSSSGALNAVSIVC
GKSVDFCCSHLLGMVGQLERLSLSILHPAYAPIEHVKQQLQDALPPDAHVLASQRLGISL
TRWPDGRNFLVTDFATCDELIQALVCTLYFPFYCGLIPPEFRGERYIDGALSNNLPFADC
PSTITVSPFHGTVDICPQSTSPNLHELNVFNFSFQISTENFFLGLICLIPPSLEVVADNC
RQGYLDALRFLERRGLTKEPVLWTLVSKEPPAPADGNWDAGCDQRWKGGLSLNWKVPHVQ
VKDVPNFEQLSPELEAALKKACTRDPSRWARFWHSGPGQVLTYLLLPCTLPFEYIYFRSR
RLVVWLPDVPADLWWMQGLLRNMALEVFSRTKAQLLGPISPPATRVLETSPLQPQIAPHR
EELGPTHQA

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

PNPLA5

Symbol Nomenclature :

PNPLA5

SYNONYM :

GS2L; dJ388M5; dJ388M5.4

Designation :

GS2-like protein|patatin-like phospholipase domain-containing protein 5

Full Nomenclature :

patatin-like phospholipase domain containing 5

GENEID :

150379

Locus Tag :

RP3-388M5.5

HGNC :

24888

MIM :

611589

Vega :

OTTHUMG00000030779

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515671      ATGGGCTTCTTAGAGGAGGAGGGCAGATGGAACCTGTCCTTCTCCGGCGCCGGCTACCTG
NM_138814.3       ATGGGCTTCTTAGAGGAGGAGGGCAGATGGAACCTGTCCTTCTCCGGCGCCGGCTACCTG
                  ************************************************************

HsCD00515671      GGCGCCCACCACGTGGGCGCCACCGAATGCCTGCGCCAGCGAGCCCCGCGCCTCCTCCAG
NM_138814.3       GGCGCCCACCACGTGGGCGCCACCGAATGCCTGCGCCAGCGAGCCCCGCGCCTCCTCCAG
                  ************************************************************

HsCD00515671      GGCGCCCGCCGCATCTACGGTTCCTCGTCTGGGGCGCTCAACGCAGTCAGCATCGTCTGC
NM_138814.3       GGCGCCCGCCGCATCTACGGTTCCTCGTCTGGGGCGCTCAACGCAGTCAGCATCGTCTGC
                  ************************************************************

HsCD00515671      GGCAAGTCGGTCGACTTCTGCTGCTCCCACCTCCTGGGCATGGTTGGGCAGTTGGAGCGG
NM_138814.3       GGCAAGTCGGTCGACTTCTGCTGCTCCCACCTCCTGGGCATGGTTGGGCAGTTGGAGCGG
                  ************************************************************

HsCD00515671      CTGAGCCTAAGCATCCTGCACCCGGCCTACGCGCCCATCGAGCACGTCAAGCAGCAGCTG
NM_138814.3       CTGAGCCTAAGCATCCTGCACCCGGCCTACGCGCCCATCGAGCACGTCAAGCAGCAGCTG
                  ************************************************************

HsCD00515671      CAGGATGCTCTGCCCCCCGACGCCCACGTCCTGGCCTCCCAGCGGCTGGGCATTTCGCTG
NM_138814.3       CAGGATGCTCTGCCCCCCGACGCCCACGTCCTGGCCTCCCAGCGGCTGGGCATTTCGCTG
                  ************************************************************

HsCD00515671      ACCCGCTGGCCTGACGGACGCAACTTCTTGGTCACTGACTTCGCCACCTGCGATGAGCTC
NM_138814.3       ACCCGCTGGCCTGACGGACGCAACTTCTTGGTCACTGACTTCGCCACCTGCGATGAGCTC
                  ************************************************************

HsCD00515671      ATCCAGGCCTTGGTCTGCACCTTATACTTTCCTTTCTACTGCGGGCTGATCCCCCCCGAG
NM_138814.3       ATCCAGGCCTTGGTCTGCACCTTATACTTTCCTTTCTACTGCGGGCTGATCCCCCCCGAG
                  ************************************************************

HsCD00515671      TTCAGAGGGGAGCGCTACATCGATGGGGCTCTGAGCAACAACTTGCCCTTTGCAGACTGC
NM_138814.3       TTCAGAGGGGAGCGCTACATCGATGGGGCTCTGAGCAACAACTTGCCCTTTGCAGACTGC
                  ************************************************************

HsCD00515671      CCCTCCACCATCACGGTGTCGCCCTTCCATGGGACAGTGGACATCTGCCCCCAGAGCACC
NM_138814.3       CCCTCCACCATCACGGTGTCGCCCTTCCATGGGACAGTGGACATCTGCCCCCAGAGCACC
                  ************************************************************

HsCD00515671      TCCCCCAACCTGCATGAGCTGAACGTCTTCAACTTCAGCTTCCAAATCTCCACTGAGAAC
NM_138814.3       TCCCCCAACCTGCATGAGCTGAACGTCTTCAACTTCAGCTTCCAAATCTCCACTGAGAAC
                  ************************************************************

HsCD00515671      TTCTTCCTGGGGCTCATATGTCTCATACCCCCCAGCCTCGAGGTAGTGGCCGACAACTGC
NM_138814.3       TTCTTCCTGGGGCTCATATGTCTCATACCCCCCAGCCTCGAGGTAGTGGCCGACAACTGC
                  ************************************************************

HsCD00515671      AGACAAGGCTACCTGGATGCCCTGAGGTTCCTGGAGAGACGTGGACTCACCAAGGAACCA
NM_138814.3       AGACAAGGCTACCTGGATGCCCTGAGGTTCCTGGAGAGACGTGGACTCACCAAGGAACCA
                  ************************************************************

HsCD00515671      GTGCTATGGACGCTGGTGTCTAAGGAACCCCCAGCCCCGGCTGACGGAAACTGGGATGCT
NM_138814.3       GTGCTATGGACGCTGGTGTCTAAGGAACCCCCAGCCCCGGCTGACGGAAACTGGGATGCT
                  ************************************************************

HsCD00515671      GGCTGTGACCAACGCTGGAAGGGGGGCCTGTCTCTCAACTGGAAAGTGCCCCATGTGCAA
NM_138814.3       GGCTGTGACCAACGCTGGAAGGGGGGCCTGTCTCTCAACTGGAAAGTGCCCCATGTGCAA
                  ************************************************************

HsCD00515671      GTCAAGGATGTACCCAACTTTGAGCAGCTCTCACCAGAGCTGGAGGCTGCACTGAAGAAA
NM_138814.3       GTCAAGGATGTACCCAACTTTGAGCAGCTCTCACCAGAGCTGGAGGCTGCACTGAAGAAA
                  ************************************************************

HsCD00515671      GCATGTACGAGGGATCCCAGCCGGTGGGCCCGCTTCTGGCACTCGGGGCCTGGACAGGTG
NM_138814.3       GCATGTACGAGGGATCCCAGCCGGTGGGCCCGCTTCTGGCACTCGGGGCCTGGACAGGTG
                  ************************************************************

HsCD00515671      CTGACGTACCTGCTGCTACCCTGCACACTGCCCTTCGAGTACATCTACTTCCGCAGCAGA
NM_138814.3       CTGACGTACCTGCTGCTACCCTGCACACTGCCCTTCGAGTACATCTACTTCCGCAGCAGA
                  ************************************************************

HsCD00515671      AGGTTGGTGGTGTGGCTGCCCGATGTGCCGGCGGACTTGTGGTGGATGCAGGGCCTGCTG
NM_138814.3       AGGTTGGTGGTGTGGCTGCCCGATGTGCCGGCGGACTTGTGGTGGATGCAGGGCCTGCTG
                  ************************************************************

HsCD00515671      AGGAACATGGCCCTCGAGGTTTTCTCCAGGACCAAGGCCCAGCTCCTTGGGCCCATCAGC
NM_138814.3       AGGAACATGGCCCTCGAGGTTTTCTCCAGGACCAAGGCCCAGCTCCTTGGGCCCATCAGC
                  ************************************************************

HsCD00515671      CCTCCGGCCACTCGCGTCCTGGAAACAAGCCCCCTCCAACCCCAGATAGCTCCTCATAGA
NM_138814.3       CCTCCGGCCACTCGCGTCCTGGAAACAAGCCCCCTCCAACCCCAGATAGCTCCTCATAGA
                  ************************************************************

HsCD00515671      GAGGAGCTCGGGCCCACCCACCAGGCC---
NM_138814.3       GAGGAGCTCGGGCCCACCCACCAGGCCTGA
                  ***************************