DNASU Plasmid | SLC17A2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: SLC17A2

Gene Name: solute carrier family 17, member 2

Original Clone ID:

ccsbBroadEn_02369

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC112033.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515676

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1309nts

Open reading frame: 1 to 1309

Reference Sequence Alignment

ATGGACGGGAAGCCTGCCACCAGGAAAGGTCCAGATTTCTGTTCATTACGCTATGGGCTG
GCTCTTATCATGCACTTCTCAAACTTCACCATGATAACGCAGCGTGTGAGTCTGAGCATT
GCGATCATCGCCATGGTGAACACCACTCAGCAGCAAGGTCTATCTAATGCCTCCACTGAG
GGGCCTGTTGCAGATGCCTTCAATAACTCCAGCATATCCATCAAGGAATTTGATACAAAG
GCCTCTGTGTATCAATGGAGCCCAGAAACTCAGGGTATCATCTTTAGCTCCATCAACTAT
GGGATAATACTGACTCTGATCCCAAGTGGATATTTAGCAGGGATATTTGGAGCAAAAAAA
ATGCTTGGTGCTGGTTTGCTGATCTCTTCCCTTCTCACCCTCTTTACACCACTGGCTGCT
GACTTCGGAGTGATTTTGGTCATCATGGTTCGGACAGTCCAGGGCATGGCCCAGGGAATG
GCATGGACAGGTCAGTTTACTATTTGGGCAAAGTGGGCTCCTCCACTTGAACGAAGCAAG
CTCACCACCATTGCAGGATCAGGGTCAGCATTTGGATCCTTCATCATCCTCTGTGTGGGG
GGACTAATCTCACAGGCCTTGAGCTGGCCTTTTATCTTCTACATCTTTGGTAGCACTGGC
TGTGTCTGCTGTCTCCTATGGTTCACAGTGATTTATGATGACCCCATGCATCACCCGTGC
ATAAGTGTTAGGGAAAAGGAGCACATCCTGTCCTCACTGGCTCAACAGCCCAGTTCTCCT
GGACGAGCTGTCCCCATAAAGGCGATGGTCACATGCCTACCACTTTGGGCCATTTTCCTG
GGTTTTTTCAGCCATTTCTGGTTGTGCACCATCATCCTAACATACCTACCAACGTATATC
AGTACTCTGCTCCATGTTAACATCAGAGATAGTGGAGTTCTGTCCTCCCTGCCTTTTATT
GCTGCTGCAAGCTGTACAATTTTAGGAGGTCAGCTGGCAGATTTCCTTTTGTCCAGGAAT
CTTCTCAGATTGATCACTGTGCGAAAGCTCTTTTCATCTCTTGATATGCAAGTTTCCTCA
TGGGAATCTCAAGGGGATTTGGGCTCATCGCAGGAATCATCTCTTCCACTGCCACTGGAT
TCCTCATCAGTCAGGATTTTGAGTCTGGTTGGAGGAATGTCTTTTTCCTGTCTGCTGCAG
TCAACATGTTTGGCCTGGTCTTTTACCTCACGTTTGGACAAGCAGAACTTCAAGACTGGG
CCAAAGAGAGGACCCTTACCCGCCTCTGAGGACATAAAGTTACAAACT

Protein sequence predicted based on nucleotide sequence

MDGKPATRKGPDFCSLRYGLALIMHFSNFTMITQRVSLSIAIIAMVNTTQQQGLSNASTE
GPVADAFNNSSISIKEFDTKASVYQWSPETQGIIFSSINYGIILTLIPSGYLAGIFGAKK
MLGAGLLISSLLTLFTPLAADFGVILVIMVRTVQGMAQGMAWTGQFTIWAKWAPPLERSK
LTTIAGSGSAFGSFIILCVGGLISQALSWPFIFYIFGSTGCVCCLLWFTVIYDDPMHHPC
ISVREKEHILSSLAQQPSSPGRAVPIKAMVTCLPLWAIFLGFFSHFWLCTIILTYLPTYI
STLLHVNIRDSGVLSSLPFIAAASCTILGGQLADFLLSRNLLRLITVRKLFSSLDMQVSS
WESQGDLGSSQESSLPLPLDSSSVRILSLVGGMSFSCLLQSTCLAWSFTSRLDKQNFKTG
PKRGPLPASEDIKLQT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

SLC17A2

Symbol Nomenclature :

SLC17A2

SYNONYM :

NPT3

Designation :

Na(+)/PI cotransporter 3|sodium phosphate transporter 3|sodium-dependent phosphate transport protein 3|sodium/phosphate cotransporter 3|solute carrier family 17 (sodium phosphate), member 2|solute carrier family 17 (vesicular glutamate transporter), member 2

Full Nomenclature :

solute carrier family 17, member 2

GENEID :

10246

HGNC :

10930

MIM :

611049

Vega :

OTTHUMG00000014413

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515676        ATGGACGGGAAGCCTGCCACCAGGAAAGGTCCAGATTTCTGTTCATTACGCTATGGGCTG
NM_001286123.1      ATGGACGGGAAGCCTGCCACCAGGAAAGGTCCAGATTTCTGTTCATTACGCTATGGGCTG
NM_005835.3         ATGGACGGGAAGCCTGCCACCAGGAAAGGTCCAGATTTCTGTTCATTACGCTATGGGCTG
                    ************************************************************

HsCD00515676        GCTCTTATCATGCACTTCTCAAACTTCACCATGATAACGCAGCGTGTGAGTCTGAGCATT
NM_001286123.1      GCTCTTATCATGCACTTCTCAAACTTCACCATGATAACGCAGCGTGTGAGTCTGAGCATT
NM_005835.3         GCTCTTATCATGCACTTCTCAAACTTCACCATGATAACGCAGCGTGTGAGTCTGAGCATT
                    ************************************************************

HsCD00515676        GCGATCATCGCCATGGTGAACACCACTCAGCAGCAAGGTCTATCTAATGCCTCCACTGAG
NM_001286123.1      GCGATCATCGCCATGGTGAACACCACTCAGCAGCAAGGTCTATCTAATGCCTCCACTGAG
NM_005835.3         GCGATCATCGCCATGGTGAACACCACTCAGCAGCAAGGTCTATCTAATGCCTCCACTGAG
                    ************************************************************

HsCD00515676        GGGCCTGTTGCAGATGCCTTCAATAACTCCAGCATATCCATCAAGGAATTTGATACAAAG
NM_001286123.1      GGGCCTGTTGCAGATGCCTTCAATAACTCCAGCATATCCATCAAGGAATTTGATACAAAG
NM_005835.3         GGGCCTGTTGCAGATGCCTTCAATAACTCCAGCATATCCATCAAGGAATTTGATACAAAG
                    ************************************************************

HsCD00515676        GCCTCTGTGTATCAATGGAGCCCAGAAACTCAGGGTATCATCTTTAGCTCCATCAACTAT
NM_001286123.1      GCCTCTGTGTATCAATGGAGCCCAGAAACTCAGGGTATCATCTTTAGCTCCATCAACTAT
NM_005835.3         GCCTCTGTGTATCAATGGAGCCCAGAAACTCAGGGTATCATCTTTAGCTCCATCAACTAT
                    ************************************************************

HsCD00515676        GGGATAATACTGACTCTGATCCCAAGTGGATATTTAGCAGGGATATTTGGAGCAAAAAAA
NM_001286123.1      GGGATAATACTGACTCTGATCCCAAGTGGATATTTAGCAGGGATATTTGGAGCAAAAAAA
NM_005835.3         GGGATAATACTGACTCTGATCCCAAGTGGATATTTAGCAGGGATATTTGGAGCAAAAAAA
                    ************************************************************

HsCD00515676        ATGCTTGGTGCTGGTTTGCTGATCTCTTCCCTTCTCACCCTCTTTACACCACTGGCTGCT
NM_001286123.1      ATGCTTGGTGCTGGTTTGCTGATCTCTTCCCTTCTCACCCTCTTTACACCACTGGCTGCT
NM_005835.3         ATGCTTGGTGCTGGTTTGCTGATCTCTTCCCTTCTCACCCTCTTTACACCACTGGCTGCT
                    ************************************************************

HsCD00515676        GACTTCGGAGTGATTTTGGTCATCATGGTTCGGACAGTCCAGGGCATGGCCCAGGGAATG
NM_001286123.1      GACTTCGGAGTGATTTTGGTCATCATGGTTCGGACAGTCCAGGGCATGGCCCAGGGAATG
NM_005835.3         GACTTCGGAGTGATTTTGGTCATCATGGTTCGGACAGTCCAGGGCATGGCCCAGGGAATG
                    ************************************************************

HsCD00515676        GCATGGACAGGTCAGTTTACTATTTGGGCAAAGTGGGCTCCTCCACTTGAACGAAGCAAG
NM_001286123.1      GCATGGACAGGTCAGTTTACTATTTGGGCAAAGTGGGCTCCTCCACTTGAACGAAGCAAG
NM_005835.3         GCATGGACAGGTCAGTTTACTATTTGGGCAAAGTGGGCTCCTCCACTTGAACGAAGCAAG
                    ************************************************************

HsCD00515676        CTCACCACCATTGCAGGATCAGGGTCAGCATTTGGATCCTTCATCATCCTCTGTGTGGGG
NM_001286123.1      CTCACCACCATTGCAGGATCAGGGTCAGCATTTGGATCCTTCATCATCCTCTGTGTGGGG
NM_005835.3         CTCACCACCATTGCAGGATCAGGGTCAGCATTTGGATCCTTCATCATCCTCTGTGTGGGG
                    ************************************************************

HsCD00515676        GGACTAATCTCACAGGCCTTGAGCTGGCCTTTTATCTTCTACATCTTTGGTAGCACTGGC
NM_001286123.1      GGACTAATCTCACAGGCCTTGAGCTGGCCTTTTATCTTCTACATCTTTGGTAGCACTGGC
NM_005835.3         GGACTAATCTCACAGGCCTTGAGCTGGCCTTTTATCTTCTACATCTTTGGTAGCACTGGC
                    ************************************************************

HsCD00515676        TGTGTCTGCTGTCTCCTATGGTTCACAGTGATTTATGATGACCCCATGCATCACCCGTGC
NM_001286123.1      TGTGTCTGCTGTCTCCTATGGTTCACAGTGATTTATGATGACCCCATGCATCACCCGTGC
NM_005835.3         TGTGTCTGCTGTCTCCTATGGTTCACAGTGATTTATGATGACCCCATGCATCACCCGTGC
                    ************************************************************

HsCD00515676        ATAAGTGTTAGGGAAAAGGAGCACATCCTGTCCTCACTGGCTCAACAGCCCAGTTCTCCT
NM_001286123.1      ATAAGTGTTAGGGAAAAGGAGCACATCCTGTCCTCACTGGCTCAACAGCCCAGTTCTCCT
NM_005835.3         ATAAGTGTTAGGGAAAAGGAGCACATCCTGTCCTCACTGGCTCAACAGCCCAGTTCTCCT
                    ************************************************************

HsCD00515676        GGACGAGCTGTCCCCATAAAGGCGATGGTCACATGCCTACCACTTTGGGCCATTTTCCTG
NM_001286123.1      GGACGAGCTGTCCCCATAAAGGCGATGGTCACATGCCTACCACTTTGGGCCATTTTCCTG
NM_005835.3         GGACGAGCTGTCCCCATAAAGGCGATGGTCACATGCCTACCACTTTGGGCCATTTTCCTG
                    ************************************************************

HsCD00515676        GGTTTTTTCAGCCATTTCTGGTTGTGCACCATCATCCTAACATACCTACCAACGTATATC
NM_001286123.1      GGTTTTTTCAGCCATTTCTGGTTGTGCACCATCATCCTAACATACCTACCAACGTATATC
NM_005835.3         GGTTTTTTCAGCCATTTCTGGTTGTGCACCATCATCCTAACATACCTACCAACGTATATC
                    ************************************************************

HsCD00515676        AGTACTCTGCTCCATGTTAACATCAGAGATAGTGGAGTTCTGTCCTCCCTGCCTTTTATT
NM_001286123.1      AGTACTCTGCTCCATGTTAACATCAGAGATAGTGGAGTTCTGTCCTCCCTGCCTTTTATT
NM_005835.3         AGTACTCTGCTCCATGTTAACATCAGAGATAGTGGAGTTCTGTCCTCCCTGCCTTTTATT
                    ************************************************************

HsCD00515676        GCTGCTGCAAGCTGTACAATTTTAGGAGGTCAGCTGGCAGATTTCCTTTTGTCCAGGAAT
NM_001286123.1      GCTGCTGCAAGCTGTACAATTTTAGGAGGTCAGCTGGCAGATTTCCTTTTGTCCAGGAAT
NM_005835.3         GCTGCTGCAAGCTGTACAATTTTAGGAGGTCAGCTGGCAGATTTCCTTTTGTCCAGGAAT
                    ************************************************************

HsCD00515676        CTTCTCAGATTGATCACTGTGCGAAAGCTCTTTTCATCTCTTG-----------------
NM_001286123.1      CTTCTCAGATTGATCACTGTGCGAAAGCTCTTTTCATCTCTTGGGCTCCTCCTTCCATCA
NM_005835.3         CTTCTCAGATTGATCACTGTGCGAAAGCTCTTTTCATCTCTTG-----------------
                    *******************************************                 

HsCD00515676        ------------------------------------------------------------
NM_001286123.1      ATATGTGCTGTGGCCCTGCCCTTTGTGGCCTCCAGTTACGTGATAACCATTATTTTGCTG
NM_005835.3         ------------------------------------------------------------
                                                                                

HsCD00515676        ------------------------------------------------------------
NM_001286123.1      ATACTTATTCCTGGGACCAGTAACCTATGTGACTCAGGGTTTATCATCAACACCTTAGAT
NM_005835.3         ------------------------------------------------------------
                                                                                

HsCD00515676        -----------ATATGCAAGTTTCCTCATGGGAATCTCAAGGGGATTTGGGCTCATCGCA
NM_001286123.1      ATCGCCCCCAGATATGCAAGTTTCCTCATGGGAATCTCAAGGGGATTTGGGCTCATCGCA
NM_005835.3         -----------ATATGCAAGTTTCCTCATGGGAATCTCAAGGGGATTTGGGCTCATCGCA
                               *************************************************

HsCD00515676        GGAATCATCTCTTCCACTGCCACTGGATTCCTCATCAGTCAGGATTTTGAGTCTGGTTGG
NM_001286123.1      GGAATCATCTCTTCCACTGCCACTGGATTCCTCATCAGTCAGGATTTTGAGTCTGGTTGG
NM_005835.3         GGAATCATCTCTTCCACTGCCACTGGATTCCTCATCAGTCAGGATTTTGAGTCTGGTTGG
                    ************************************************************

HsCD00515676        AGGAATGTCTTTTTCCTGTCTGCTGCAGTCAACATGTTTGGCCTGGTCTTTTACCTCACG
NM_001286123.1      AGGAATGTCTTTTTCCTGTCTGCTGCAGTCAACATGTTTGGCCTGGTCTTTTACCTCACG
NM_005835.3         AGGAATGTCTTTTTCCTGTCTGCTGCAGTCAACATGTTTGGCCTGGTCTTTTACCTCACG
                    ************************************************************

HsCD00515676        TTTGGACAAGCAGAACTTCAAGACTGGGCCAAAGAGAGGACCCTTACCCGCCTCTGAGGA
NM_001286123.1      TTTGGACAAGCAGAACTTCAAGACTGGGCCAAAGAGAGGACCCTTACCCGCCTCTGA---
NM_005835.3         TTTGGACAAGCAGAACTTCAAGACTGGGCCAAAGAGAGGACCCTTACCCGCCTCTGAGGA
                    *********************************************************   

HsCD00515676        CATAAAGTTACAAACT---
NM_001286123.1      -------------------
NM_005835.3         CATAAAGTTACAAACTTAA