DNASU Plasmid | ZNF491 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF491

Gene Name: zinc finger protein 491

Original Clone ID:

ccsbBroadEn_04807

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC101590.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515678

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1312nts

Open reading frame: 1 to 1312

Reference Sequence Alignment

ATGGGAGAGAGACTCTTTGAGAGTGCAGAAGGTAGTCAGTGTGGAGAAACTTTCACCCAG
GTTCCGGAAGACATGCTGAACAAGAAAACTCTTCCTGGAGTAAAATCATGTGAAAGTGGT
ACATGTGGAGAAATCTTCATGGGATATTCATCCTTTAATAGGAACATCAGAACTGACACT
GGACACCAACCACATAAGTGTCAGAAATTTTTAGAGAAGCCATATAAACATAAACAACGT
AGGAAAGCCTTGAGCCATAGCCACTGCTTTCGAACACATGAAAGGCCTCACACTAGAGAG
AAACCTTTTGATTGTAAGGAATGTGAAAAATCTTTCATTTCTCCTGCAAGCATTCGAAGA
TATATGGTAACGCACAGTGGAGATGGACCTTATAAATGTAAGTTTTGTGGGAAAGCCTTG
GATTGTCTCAGTTTATACCTTACCCATGAACGAACTCACACTGGAGAGAAACGATATGAA
TGTAAACAATGTGGTAAAGCCTTCAGTTGGCACAGTTCTGTTCGAATCCATGAAAGAACT
CACACTGGGGAGAAGCCATATGAATGTAAGGAGTGTGGGAAATCATTCAATTTTTCCAGT
TCCTTTCGCAGACATGAAAGGACACACACAGGAGAGAAGCCGTACAAATGTAAGGAATGT
GGGAAAGCCTTCAATTGTCCCAGTTCTTTTCACAGGCATGAAAGGACTCACACAGGAGAA
AAACCCTATGAATGTAAACTATATGGGAAAGCATTATCTCGCCTTATAAGCTTTCGAAGA
CACATGAGAATGCACACTGGAGAGAGGCCTCATAAATGTAAGATATGTGGGAAAGCCTTT
TACTCTCCCAGTTCATTTCAAAGGCATGAAAGAAGTCACACTGGAGAGAAACCCTACAAA
TGCAAGCAATGTGGGAAAGCCTTCACTTGTTCCACTTCGTTTCAATATCATGAAAGGACT
CACACTGGAGAGAAACCCGATGGGTGTAAGCAATGTGGGAAAGCCTTCAGATCTGCCAAG
TACATTCGAATACATGGAAGGACTCACACTGGTGAGAAACCCTATGAATGTAAGCAATGT
GGGAAAGCATTTCATTGTGTCAGCTCCTTTCATAGACATGAAAGGACTCACGCTGGAGAA
AAACCTTATGAATGTAAGCATTGTGGGAAAGCCTTCACTTGTTCCATATATATTAGAATA
CATGAAAGAATTCACACTGGAGAGAAACCTTACCAATGTAAGGAATGTGGGAAAGCCTTC
ATTCGTTCCAGTTACTGTCGAAAACATGAAAGAACTCACACTATTAATATA

Protein sequence predicted based on nucleotide sequence

MGERLFESAEGSQCGETFTQVPEDMLNKKTLPGVKSCESGTCGEIFMGYSSFNRNIRTDT
GHQPHKCQKFLEKPYKHKQRRKALSHSHCFRTHERPHTREKPFDCKECEKSFISPASIRR
YMVTHSGDGPYKCKFCGKALDCLSLYLTHERTHTGEKRYECKQCGKAFSWHSSVRIHERT
HTGEKPYECKECGKSFNFSSSFRRHERTHTGEKPYKCKECGKAFNCPSSFHRHERTHTGE
KPYECKLYGKALSRLISFRRHMRMHTGERPHKCKICGKAFYSPSSFQRHERSHTGEKPYK
CKQCGKAFTCSTSFQYHERTHTGEKPDGCKQCGKAFRSAKYIRIHGRTHTGEKPYECKQC
GKAFHCVSSFHRHERTHAGEKPYECKHCGKAFTCSIYIRIHERIHTGEKPYQCKECGKAF
IRSSYCRKHERTHTINI

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF491

Symbol Nomenclature :

ZNF491

Full Nomenclature :

zinc finger protein 491

GENEID :

126069

HGNC :

23706

Vega :

OTTHUMG00000156529

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515678        ATGGGAGAGAGACTCTTTGAGAGTGCAGAAGGTAGTCAGTGTGGAGAAACTTTCACCCAG
XM_005259730.1      ATGGGAGAGAGACTCTTTGAGAGTGCAGAAGGTAGTCAGTGTGGAGAAACTTTCACCCAG
NM_152356.3         ATGGGAGAGAGACTCTTTGAGAGTGCAGAAGGTAGTCAGTGTGGAGAAACTTTCACCCAG
                    ************************************************************

HsCD00515678        GTTCCGGAAGACATGCTGAACAAGAAAACTCTTCCTGGAGTAAAATCATGTGAAAGTGGT
XM_005259730.1      GTTCCGGAAGACATGCTGAACAAGAAAACTCTTCCTGGAGTAAAATCATGTGAAAGTGGT
NM_152356.3         GTTCCGGAAGACATGCTGAACAAGAAAACTCTTCCTGGAGTAAAATCATGTGAAAGTGGT
                    ************************************************************

HsCD00515678        ACATGTGGAGAAATCTTCATGGGATATTCATCCTTTAATAGGAACATCAGAACTGACACT
XM_005259730.1      ACATGTGGAGAAATCTTCATGGGATATTCATCCTTTAATAGGAACATCAGAACTGACACT
NM_152356.3         ACATGTGGAGAAATCTTCATGGGATATTCATCCTTTAATAGGAACATCAGAACTGACACT
                    ************************************************************

HsCD00515678        GGACACCAACCACATAAGTGTCAGAAATTTTTAGAGAAGCCATATAAACATAAACAACGT
XM_005259730.1      GGACACCAACCACATAAGTGTCAGAAATTTTTAGAGAAGCCATATAAACATAAACAACGT
NM_152356.3         GGACACCAACCACATAAGTGTCAGAAATTTTTAGAGAAGCCATATAAACATAAACAACGT
                    ************************************************************

HsCD00515678        AGGAAAGCCTTGAGCCATAGCCACTGCTTTCGAACACATGAAAGGCCTCACACTAGAGAG
XM_005259730.1      AGGAAAGCCTTGAGCCATAGCCACTGCTTTCGAACACATGAAAGGCCTCACACTAGAGAG
NM_152356.3         AGGAAAGCCTTGAGCCATAGCCACTGCTTTCGAACACATGAAAGGCCTCACACTAGAGAG
                    ************************************************************

HsCD00515678        AAACCTTTTGATTGTAAGGAATGTGAAAAATCTTTCATTTCTCCTGCAAGCATTCGAAGA
XM_005259730.1      AAACCTTTTGATTGTAAGGAATGTGAAAAATCTTTCATTTCTCCTGCAAGCATTCGAAGA
NM_152356.3         AAACCTTTTGATTGTAAGGAATGTGAAAAATCTTTCATTTCTCCTGCAAGCATTCGAAGA
                    ************************************************************

HsCD00515678        TATATGGTAACGCACAGTGGAGATGGACCTTATAAATGTAAGTTTTGTGGGAAAGCCTTG
XM_005259730.1      TATATGGTAACGCACAGTGGAGATGGACCTTATAAATGTAAGTTTTGTGGGAAAGCCTTG
NM_152356.3         TATATGGTAACGCACAGTGGAGATGGACCTTATAAATGTAAGTTTTGTGGGAAAGCCTTG
                    ************************************************************

HsCD00515678        GATTGTCTCAGTTTATACCTTACCCATGAACGAACTCACACTGGAGAGAAACGATATGAA
XM_005259730.1      GATTGTCTCAGTTTATACCTTACCCATGAACGAACTCACACTGGAGAGAAACGATATGAA
NM_152356.3         GATTGTCTCAGTTTATACCTTACCCATGAACGAACTCACACTGGAGAGAAACGATATGAA
                    ************************************************************

HsCD00515678        TGTAAACAATGTGGTAAAGCCTTCAGTTGGCACAGTTCTGTTCGAATCCATGAAAGAACT
XM_005259730.1      TGTAAACAATGTGGTAAAGCCTTCAGTTGGCACAGTTCTGTTCGAATCCATGAAAGAACT
NM_152356.3         TGTAAACAATGTGGTAAAGCCTTCAGTTGGCACAGTTCTGTTCGAATCCATGAAAGAACT
                    ************************************************************

HsCD00515678        CACACTGGGGAGAAGCCATATGAATGTAAGGAGTGTGGGAAATCATTCAATTTTTCCAGT
XM_005259730.1      CACACTGGGGAGAAGCCATATGAATGTAAGGAGTGTGGGAAATCATTCAATTTTTCCAGT
NM_152356.3         CACACTGGGGAGAAGCCATATGAATGTAAGGAGTGTGGGAAATCATTCAATTTTTCCAGT
                    ************************************************************

HsCD00515678        TCCTTTCGCAGACATGAAAGGACACACACAGGAGAGAAGCCGTACAAATGTAAGGAATGT
XM_005259730.1      TCCTTTCGCAGACATGAAAGGACACACACAGGAGAGAAGCCGTACAAATGTAAGGAATGT
NM_152356.3         TCCTTTCGCAGACATGAAAGGACACACACAGGAGAGAAGCCGTACAAATGTAAGGAATGT
                    ************************************************************

HsCD00515678        GGGAAAGCCTTCAATTGTCCCAGTTCTTTTCACAGGCATGAAAGGACTCACACAGGAGAA
XM_005259730.1      GGGAAAGCCTTCAATTGTCCCAGTTCTTTTCACAGGCATGAAAGGACTCACACAGGAGAA
NM_152356.3         GGGAAAGCCTTCAATTGTCCCAGTTCTTTTCACAGGCATGAAAGGACTCACACAGGAGAA
                    ************************************************************

HsCD00515678        AAACCCTATGAATGTAAACTATATGGGAAAGCATTATCTCGCCTTATAAGCTTTCGAAGA
XM_005259730.1      AAACCCTATGAATGTAAACTATATGGGAAAGCATTATCTCGCCTTATAAGCTTTCGAAGA
NM_152356.3         AAACCCTATGAATGTAAACTATATGGGAAAGCATTATCTCGCCTTATAAGCTTTCGAAGA
                    ************************************************************

HsCD00515678        CACATGAGAATGCACACTGGAGAGAGGCCTCATAAATGTAAGATATGTGGGAAAGCCTTT
XM_005259730.1      CACATGAGAATGCACACTGGAGAGAGGCCTCATAAATGTAAGATATGTGGGAAAGCCTTT
NM_152356.3         CACATGAGAATGCACACTGGAGAGAGGCCTCATAAATGTAAGATATGTGGGAAAGCCTTT
                    ************************************************************

HsCD00515678        TACTCTCCCAGTTCATTTCAAAGGCATGAAAGAAGTCACACTGGAGAGAAACCCTACAAA
XM_005259730.1      TACTCTCCCAGTTCATTTCAAAGGCATGAAAGAAGTCACACTGGAGAGAAACCCTACAAA
NM_152356.3         TACTCTCCCAGTTCATTTCAAAGGCATGAAAGAAGTCACACTGGAGAGAAACCCTACAAA
                    ************************************************************

HsCD00515678        TGCAAGCAATGTGGGAAAGCCTTCACTTGTTCCACTTCGTTTCAATATCATGAAAGGACT
XM_005259730.1      TGCAAGCAATGTGGGAAAGCCTTCACTTGTTCCACTTCGTTTCAATATCATGAAAGGACT
NM_152356.3         TGCAAGCAATGTGGGAAAGCCTTCACTTGTTCCACTTCGTTTCAATATCATGAAAGGACT
                    ************************************************************

HsCD00515678        CACACTGGAGAGAAACCCGATGGGTGTAAGCAATGTGGGAAAGCCTTCAGATCTGCCAAG
XM_005259730.1      CACACTGGAGAGAAACCCGATGGGTGTAAGCAATGTGGGAAAGCCTTCAGATCTGCCAAG
NM_152356.3         CACACTGGAGAGAAACCCGATGGGTGTAAGCAATGTGGGAAAGCCTTCAGATCTGCCAAG
                    ************************************************************

HsCD00515678        TACATTCGAATACATGGAAGGACTCACACTGGTGAGAAACCCTATGAATGTAAGCAATGT
XM_005259730.1      TACATTCGAATACATGGAAGGACTCACACTGGTGAGAAACCCTATGAATGTAAGCAATGT
NM_152356.3         TACATTCGAATACATGGAAGGACTCACACTGGTGAGAAACCCTATGAATGTAAGCAATGT
                    ************************************************************

HsCD00515678        GGGAAAGCATTTCATTGTGTCAGCTCCTTTCATAGACATGAAAGGACTCACGCTGGAGAA
XM_005259730.1      GGGAAAGCATTTCATTGTGTCAGCTCCTTTCATAGACATGAAAGGACTCACGCTGGAGAA
NM_152356.3         GGGAAAGCATTTCATTGTGTCAGCTCCTTTCATAGACATGAAAGGACTCACGCTGGAGAA
                    ************************************************************

HsCD00515678        AAACCTTATGAATGTAAGCATTGTGGGAAAGCCTTCACTTGTTCCATATATATTAGAATA
XM_005259730.1      AAACCTTATGAATGTAAGCATTGTGGGAAAGCCTTCACTTGTTCCATATATATTAGAATA
NM_152356.3         AAACCTTATGAATGTAAGCATTGTGGGAAAGCCTTCACTTGTTCCATATATATTAGAATA
                    ************************************************************

HsCD00515678        CATGAAAGAATTCACACTGGAGAGAAACCTTACCAATGTAAGGAATGTGGGAAAGCCTTC
XM_005259730.1      CATGAAAGAATTCACACTGGAGAGAAACCTTACCAATGTAAGGAATGTGGGAAAGCCTTC
NM_152356.3         CATGAAAGAATTCACACTGGAGAGAAACCTTACCAATGTAAGGAATGTGGGAAAGCCTTC
                    ************************************************************

HsCD00515678        ATTCGTTCCAGTTACTGTCGAAAACATGAAAGAACTCACACTATTAATATA---
XM_005259730.1      ATTCGTTCCAGTTACTGTCGAAAACATGAAAGAACTCACACTATTAATATATGA
NM_152356.3         ATTCGTTCCAGTTACTGTCGAAAACATGAAAGAACTCACACTATTAATATATGA
                    ***************************************************