DNASU Plasmid | CCDC6 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: CCDC6

Gene Name: coiled-coil domain containing 6

Original Clone ID:

ccsbBroadEn_07195

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC064391.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515745

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1423nts

Open reading frame: 1 to 1423

Reference Sequence Alignment

ATGGCGGACAGCGCCAGCGAGAGCGACACGGACGGGGCGGGGGGCAACAGCAGCAGCTCG
GCCGCCATGCAGTCGTCCTGCTCGTCGACCTCGGGCGGCGGCGGTGGCGGCGGGGGAGGC
GGCGGCGGTGGGAAGTCGGGGGGCATTGTCATCTCGCCGTTCCGCCTGGAGGAGCTCACC
AACCGCCTGGCCTCGCTGCAGCAAGAGAACAAGGTGCTGAAGATAGAGCTGGAGACCTAC
AAACTGAAGTGCAAGGCACTGCAGGAGGAGAACCGCGACCTGCGCAAAGCCAGCGTTACC
ATCCAAGCCAGGGCTGAGCAGGAAGAAGAATTCATTAGTAACACTTTATTCAAGAAAATT
CAGGCTTTGCAGAAGGAGAAAGAAACCCTTGCTGTAAATTATGAGAAAGAAGAAGAATTC
CTCACTAATGAGCTCTCCAGAAAATTGATGCAGTTGCAGCATGAGAAAGCCGAACTAGAA
CAGCATCTTGAACAAGAGCAGGAATTTCAGGTCAACAAACTGATGAAGAAAATTAAAAAA
CTGGAGAATGACACCATTTCTAAGCAACTTACATTAGAACAGTTGAGACGGGAGAAGATT
GACCTTGAAAATACATTGGAACAAGAACAAGAAGCACTAGTTAATCGCCTCTGGAAAAGG
ATGGATAAGCTTGAAGCTGAAAAGCGAATCCTGCAGGAAAAATTAGACCAGCCCGTCTCT
GCTCCACCATCGCCTAGAGATATCTCCATGGAGATTGATTCTCCAGAAAATATGATGCGT
CACATCAGGTTTTTAAAGAATGAAGTGGAACGGCTGAAGAAGCAACTGAGAGCTGCTCAG
TTACAGCATTCAGAGAAAATGGCACAGTATCTGGAGGAGGAACGTCACATGAGAGAAGAG
AACTTGAGGCTCCAGAGGAAGCTGCAGAGGGAGATGGAGAGAAGAGAAGCCCTCTGTCGA
CAGCTCTCCGAGAGTGAGTCCAGCTTAGAAATGGACGACGAAAGGTATTTTAATGAGATG
TCTGCACAAGGATTAAGACCTCGCACTGTGTCCAGCCCGATCCCTTACACACCTTCTCCG
AGTTCAAGCAGGCCTATATCACCTGGTCTATCATATGCAAGTCACACGGTTGGTTTCACG
CCACCAACTTCACTGACTAGAGCTGGAATGTCTTATTACAATTCCCCGGGTCTTCACGTG
CAGCACATGGGAACATCCCATGGTATCACAAGGCCTTCACCACGGAGAAGCAACAGTCCT
GACAAATTCAAACGGCCCACGCCGCCTCCATCTCCCAACACACAGACCCCAGTCCAGCCA
CCTCCACCTCCACCTCCGCCACCCATGCAGCCCACGGTCCCCTCAGCAGCCACCTCGCAG
CCTACTCCTTCGCAACATTCGGCGCACACCTCCTCCCAGCCT

Protein sequence predicted based on nucleotide sequence

MADSASESDTDGAGGNSSSSAAMQSSCSSTSGGGGGGGGGGGGGKSGGIVISPFRLEELT
NRLASLQQENKVLKIELETYKLKCKALQEENRDLRKASVTIQARAEQEEEFISNTLFKKI
QALQKEKETLAVNYEKEEEFLTNELSRKLMQLQHEKAELEQHLEQEQEFQVNKLMKKIKK
LENDTISKQLTLEQLRREKIDLENTLEQEQEALVNRLWKRMDKLEAEKRILQEKLDQPVS
APPSPRDISMEIDSPENMMRHIRFLKNEVERLKKQLRAAQLQHSEKMAQYLEEERHMREE
NLRLQRKLQREMERREALCRQLSESESSLEMDDERYFNEMSAQGLRPRTVSSPIPYTPSP
SSSRPISPGLSYASHTVGFTPPTSLTRAGMSYYNSPGLHVQHMGTSHGITRPSPRRSNSP
DKFKRPTPPPSPNTQTPVQPPPPPPPPPMQPTVPSAATSQPTPSQHSAHTSSQP

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

CCDC6

Symbol Nomenclature :

CCDC6

SYNONYM :

D10S170; H4; PTC; TPC; TST1

Designation :

coiled-coil domain-containing protein 6|papillary thyroid carcinoma-encoded protein

Full Nomenclature :

coiled-coil domain containing 6

GENEID :

8030

HGNC :

18782

MIM :

601985

Vega :

OTTHUMG00000018284

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515745      ATGGCGGACAGCGCCAGCGAGAGCGACACGGACGGGGCGGGGGGCAACAGCAGCAGCTCG
NM_005436.4       ATGGCGGACAGCGCCAGCGAGAGCGACACGGACGGGGCGGGGGGCAACAGCAGCAGCTCG
                  ************************************************************

HsCD00515745      GCCGCCATGCAGTCGTCCTGCTCGTCGACCTCGGGCGGCGGCGGTGGCGGCGGGGGAGGC
NM_005436.4       GCCGCCATGCAGTCGTCCTGCTCGTCGACCTCGGGCGGCGGCGGTGGCGGCGGGGGAGGC
                  ************************************************************

HsCD00515745      GGCGGCGGTGGGAAGTCGGGGGGCATTGTCATCTCGCCGTTCCGCCTGGAGGAGCTCACC
NM_005436.4       GGCGGCGGTGGGAAGTCGGGGGGCATTGTCATCTCGCCGTTCCGCCTGGAGGAGCTCACC
                  ************************************************************

HsCD00515745      AACCGCCTGGCCTCGCTGCAGCAAGAGAACAAGGTGCTGAAGATAGAGCTGGAGACCTAC
NM_005436.4       AACCGCCTGGCCTCGCTGCAGCAAGAGAACAAGGTGCTGAAGATAGAGCTGGAGACCTAC
                  ************************************************************

HsCD00515745      AAACTGAAGTGCAAGGCACTGCAGGAGGAGAACCGCGACCTGCGCAAAGCCAGCGTTACC
NM_005436.4       AAACTGAAGTGCAAGGCACTGCAGGAGGAGAACCGCGACCTGCGCAAAGCCAGCGTGACC
                  ******************************************************** ***

HsCD00515745      ATCCAAGCCAGGGCTGAGCAGGAAGAAGAATTCATTAGTAACACTTTATTCAAGAAAATT
NM_005436.4       ATCCAAGCCAGGGCTGAGCAGGAAGAAGAATTCATTAGTAACACTTTATTCAAGAAAATT
                  ************************************************************

HsCD00515745      CAGGCTTTGCAGAAGGAGAAAGAAACCCTTGCTGTAAATTATGAGAAAGAAGAAGAATTC
NM_005436.4       CAGGCTTTGCAGAAGGAGAAAGAAACCCTTGCTGTAAATTATGAGAAAGAAGAAGAATTC
                  ************************************************************

HsCD00515745      CTCACTAATGAGCTCTCCAGAAAATTGATGCAGTTGCAGCATGAGAAAGCCGAACTAGAA
NM_005436.4       CTCACTAATGAGCTCTCCAGAAAATTGATGCAGTTGCAGCATGAGAAAGCCGAACTAGAA
                  ************************************************************

HsCD00515745      CAGCATCTTGAACAAGAGCAGGAATTTCAGGTCAACAAACTGATGAAGAAAATTAAAAAA
NM_005436.4       CAGCATCTTGAACAAGAGCAGGAATTTCAGGTCAACAAACTGATGAAGAAAATTAAAAAA
                  ************************************************************

HsCD00515745      CTGGAGAATGACACCATTTCTAAGCAACTTACATTAGAACAGTTGAGACGGGAGAAGATT
NM_005436.4       CTGGAGAATGACACCATTTCTAAGCAACTTACATTAGAACAGTTGAGACGGGAGAAGATT
                  ************************************************************

HsCD00515745      GACCTTGAAAATACATTGGAACAAGAACAAGAAGCACTAGTTAATCGCCTCTGGAAAAGG
NM_005436.4       GACCTTGAAAATACATTGGAACAAGAACAAGAAGCACTAGTTAATCGCCTCTGGAAAAGG
                  ************************************************************

HsCD00515745      ATGGATAAGCTTGAAGCTGAAAAGCGAATCCTGCAGGAAAAATTAGACCAGCCCGTCTCT
NM_005436.4       ATGGATAAGCTTGAAGCTGAAAAGCGAATCCTGCAGGAAAAATTAGACCAGCCCGTCTCT
                  ************************************************************

HsCD00515745      GCTCCACCATCGCCTAGAGATATCTCCATGGAGATTGATTCTCCAGAAAATATGATGCGT
NM_005436.4       GCTCCACCATCGCCTAGAGATATCTCCATGGAGATTGATTCTCCAGAAAATATGATGCGT
                  ************************************************************

HsCD00515745      CACATCAGGTTTTTAAAGAATGAAGTGGAACGGCTGAAGAAGCAACTGAGAGCTGCTCAG
NM_005436.4       CACATCAGGTTTTTAAAGAATGAAGTGGAACGGCTGAAGAAGCAACTGAGAGCTGCTCAG
                  ************************************************************

HsCD00515745      TTACAGCATTCAGAGAAAATGGCACAGTATCTGGAGGAGGAACGTCACATGAGAGAAGAG
NM_005436.4       TTACAGCATTCAGAGAAAATGGCACAGTATCTGGAGGAGGAACGTCACATGAGAGAAGAG
                  ************************************************************

HsCD00515745      AACTTGAGGCTCCAGAGGAAGCTGCAGAGGGAGATGGAGAGAAGAGAAGCCCTCTGTCGA
NM_005436.4       AACTTGAGGCTCCAGAGGAAGCTGCAGAGGGAGATGGAGAGAAGAGAAGCCCTCTGTCGA
                  ************************************************************

HsCD00515745      CAGCTCTCCGAGAGTGAGTCCAGCTTAGAAATGGACGACGAAAGGTATTTTAATGAGATG
NM_005436.4       CAGCTCTCCGAGAGTGAGTCCAGCTTAGAAATGGACGACGAAAGGTATTTTAATGAGATG
                  ************************************************************

HsCD00515745      TCTGCACAAGGATTAAGACCTCGCACTGTGTCCAGCCCGATCCCTTACACACCTTCTCCG
NM_005436.4       TCTGCACAAGGATTAAGACCTCGCACTGTGTCCAGCCCGATCCCTTACACACCTTCTCCG
                  ************************************************************

HsCD00515745      AGTTCAAGCAGGCCTATATCACCTGGTCTATCATATGCAAGTCACACGGTTGGTTTCACG
NM_005436.4       AGTTCAAGCAGGCCTATATCACCTGGTCTATCATATGCAAGTCACACGGTTGGTTTCACG
                  ************************************************************

HsCD00515745      CCACCAACTTCACTGACTAGAGCTGGAATGTCTTATTACAATTCCCCGGGTCTTCACGTG
NM_005436.4       CCACCAACTTCACTGACTAGAGCTGGAATGTCTTATTACAATTCCCCGGGTCTTCACGTG
                  ************************************************************

HsCD00515745      CAGCACATGGGAACATCCCATGGTATCACAAGGCCTTCACCACGGAGAAGCAACAGTCCT
NM_005436.4       CAGCACATGGGAACATCCCATGGTATCACAAGGCCTTCACCACGGAGAAGCAACAGTCCT
                  ************************************************************

HsCD00515745      GACAAATTCAAACGGCCCACGCCGCCTCCATCTCCCAACACACAGACCCCAGTCCAGCCA
NM_005436.4       GACAAATTCAAACGGCCCACGCCGCCTCCATCTCCCAACACACAGACCCCAGTCCAGCCA
                  ************************************************************

HsCD00515745      CCTCCACCTCCACCTCCGCCACCCATGCAGCCCACGGTCCCCTCAGCAGCCACCTCGCAG
NM_005436.4       CCTCCGCCTCCACCTCCGCCACCCATGCAGCCCACGGTCCCCTCAGCAGCCACCTCGCAG
                  *****.******************************************************

HsCD00515745      CCTACTCCTTCGCAACATTCGGCGCACACCTCCTCCCAGCCT---
NM_005436.4       CCTACTCCTTCGCAACATTCGGCGCACCCCTCCTCCCAGCCTTAA
                  ***************************.**************