DNASU Plasmid | MGAT4C (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: MGAT4C

Gene Name: mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative)

Original Clone ID:

ccsbBroadEn_07949

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC064141.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515815

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1435nts

Open reading frame: 1 to 1435

Reference Sequence Alignment

ATGTTTAAATTTCATCAAATGAAACATATTTTTGAAATACTTGATAAAATGAGATGCCTG
AGAAAACGTTCTACAGTGTCATTCTTGGGAGTTCTTGTCATTTTTCTCCTTTTTATGAAC
TTGTACATTGAAGATAGCTATGTTCTGGAAGGAGACAAACAACTTATAAGGGAAACATCC
ACACATCAACTGAATTCAGAACGCTATGTTCATACTTTCAAGGATTTATCTAATTTCTCA
GGAGCCATAAATGTCACCTATCGCTACCTAGCTGCCACACCTTTACAAAGAAAGCGGTAT
CTTACAATTGGACTTTCTTCAGTAAAGCGAAAAAAAGGAAACTATTTACTTGAGACAATT
AAGTCAATTTTTGAGCAATCCAGCTATGAAGAGCTGAAGGAAATTTCAGTGGTGGTTCAC
CTAGCAGACTTTAATTCTTCCTGGCGTGATGCCATGGTCCAGGATATTACACAGAAATTT
GCGCACCATATTATTGCAGGAAGATTAATGGTTATACATGCTCCAGAGGAGTATTACCCA
ATCCTAGATGGCCTTAAAAGAAATTACAATGATCCAGAAGATAGAGTCAAATTTCGTTCC
AAGCAAAATGTAGATTATGCTTTTCTGCTTAATTTTTGTGCCAATACTTCAGACTATTAT
GTAATGCTTGAAGATGATGTTCGATGTTCAAAAAATTTCTTAACTGCCATCAAGAAAGTC
ATTGCATCCCTAGAAGGAACTTACTGGGTAACTCTTGAATTCTCTAAGCTTGGCTACATT
GGTAAACTCTATCATTCTCATGATCTCCCACGTTTGGCCCATTTTTTATTAATGTTTTAT
CAAGAAATGCCTTGTGATTGGCTATTGACTCATTTCCGTGGTCTGTTGGCTCAGAAAAAT
GTGATCCGTTTTAAACCATCTCTCTTTCAGCACATGGGCTATTATTCATCATACAAAGGG
ACGGAGAATAAGCTGAAGGATGATGATTTTGAAGAGGAGTCATTTGACATTCCTGATAAC
CCCCCTGCAAGTCTGTACACCAACATGAATGTGTTTGAAAATTATGAAGCAAGCAAGGCT
TACAGTAGTGTTGATGAGTACTTTTGGGGGAAACCACCTTCAACAGGAGATGTTTTTGTG
ATTGTATTTGAAAATCCAATTATAATAAAAAAAATTAAAGTAAATACTGGAACAGAAGAT
CGGCAAAATGATATTTTGCATCATGGAGCCCTAGATGTTGGGGAAAACGTTATGCCTAGC
AAACAAAGGAGACAATGTTCTAGTTACTTAAGACTAGGAGAATTCAAAAATGGAAACTTT
GAAATGTCAGGTGTAAATCAAAAAATTCCATTTGATATACATTGTATGAGGATATATGTC
ACCAAAACACAAAAGGAATGGCTAATTATTAGGAGTATTAGCATTTGGACTTCT

Protein sequence predicted based on nucleotide sequence

MFKFHQMKHIFEILDKMRCLRKRSTVSFLGVLVIFLLFMNLYIEDSYVLEGDKQLIRETS
THQLNSERYVHTFKDLSNFSGAINVTYRYLAATPLQRKRYLTIGLSSVKRKKGNYLLETI
KSIFEQSSYEELKEISVVVHLADFNSSWRDAMVQDITQKFAHHIIAGRLMVIHAPEEYYP
ILDGLKRNYNDPEDRVKFRSKQNVDYAFLLNFCANTSDYYVMLEDDVRCSKNFLTAIKKV
IASLEGTYWVTLEFSKLGYIGKLYHSHDLPRLAHFLLMFYQEMPCDWLLTHFRGLLAQKN
VIRFKPSLFQHMGYYSSYKGTENKLKDDDFEEESFDIPDNPPASLYTNMNVFENYEASKA
YSSVDEYFWGKPPSTGDVFVIVFENPIIIKKIKVNTGTEDRQNDILHHGALDVGENVMPS
KQRRQCSSYLRLGEFKNGNFEMSGVNQKIPFDIHCMRIYVTKTQKEWLIIRSISIWTS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

MGAT4C

Symbol Nomenclature :

MGAT4C

SYNONYM :

GNTIVH; HGNT-IV-H

Designation :

N-acetylglucosaminyltransferase IV homolog|N-acetylglucosaminyltransferase IVc|N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVc|UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVc|UDP-N-acetylglucosamine:a-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IV|UDP-N-acetylglucosamine:a-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IV-homolog|alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C|glcNAc-T IVc|gnT-IVc

Full Nomenclature :

mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative)

GENEID :

25834

HGNC :

30871

MIM :

607385

Vega :

OTTHUMG00000169846

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515815      ATGTTTAAATTTCATCAAATGAAACATATTTTTGAAATACTTGATAAAATGAGATGCCTG
NM_013244.3       ATGTTTAAATTTCATCAAATGAAACATATTTTTGAAATACTTGATAAAATGAGATGCCTG
                  ************************************************************

HsCD00515815      AGAAAACGTTCTACAGTGTCATTCTTGGGAGTTCTTGTCATTTTTCTCCTTTTTATGAAC
NM_013244.3       AGAAAACGTTCTACAGTGTCATTCTTGGGAGTTCTTGTCATTTTTCTCCTTTTTATGAAC
                  ************************************************************

HsCD00515815      TTGTACATTGAAGATAGCTATGTTCTGGAAGGAGACAAACAACTTATAAGGGAAACATCC
NM_013244.3       TTGTACATTGAAGATAGCTATGTTCTGGAAGGAGACAAACAACTTATAAGGGAAACATCC
                  ************************************************************

HsCD00515815      ACACATCAACTGAATTCAGAACGCTATGTTCATACTTTCAAGGATTTATCTAATTTCTCA
NM_013244.3       ACACATCAACTGAATTCAGAACGCTATGTTCATACTTTCAAGGATTTATCTAATTTCTCA
                  ************************************************************

HsCD00515815      GGAGCCATAAATGTCACCTATCGCTACCTAGCTGCCACACCTTTACAAAGAAAGCGGTAT
NM_013244.3       GGAGCCATAAATGTCACCTATCGCTACCTAGCTGCCACACCTTTACAAAGAAAGCGGTAT
                  ************************************************************

HsCD00515815      CTTACAATTGGACTTTCTTCAGTAAAGCGAAAAAAAGGAAACTATTTACTTGAGACAATT
NM_013244.3       CTTACAATTGGACTTTCTTCAGTAAAGCGAAAAAAAGGAAACTATTTACTTGAGACAATT
                  ************************************************************

HsCD00515815      AAGTCAATTTTTGAGCAATCCAGCTATGAAGAGCTGAAGGAAATTTCAGTGGTGGTTCAC
NM_013244.3       AAGTCAATTTTTGAGCAATCCAGCTATGAAGAGCTGAAGGAAATTTCAGTGGTGGTTCAC
                  ************************************************************

HsCD00515815      CTAGCAGACTTTAATTCTTCCTGGCGTGATGCCATGGTCCAGGATATTACACAGAAATTT
NM_013244.3       CTAGCAGACTTTAATTCTTCCTGGCGTGATGCCATGGTCCAGGATATTACACAGAAATTT
                  ************************************************************

HsCD00515815      GCGCACCATATTATTGCAGGAAGATTAATGGTTATACATGCTCCAGAGGAGTATTACCCA
NM_013244.3       GCGCACCATATTATTGCAGGAAGATTAATGGTTATACATGCTCCAGAGGAGTATTACCCA
                  ************************************************************

HsCD00515815      ATCCTAGATGGCCTTAAAAGAAATTACAATGATCCAGAAGATAGAGTCAAATTTCGTTCC
NM_013244.3       ATCCTAGATGGCCTTAAAAGAAATTACAATGATCCAGAAGATAGAGTCAAATTTCGTTCC
                  ************************************************************

HsCD00515815      AAGCAAAATGTAGATTATGCTTTTCTGCTTAATTTTTGTGCCAATACTTCAGACTATTAT
NM_013244.3       AAGCAAAATGTAGATTATGCTTTTCTGCTTAATTTTTGTGCCAATACTTCAGACTATTAT
                  ************************************************************

HsCD00515815      GTAATGCTTGAAGATGATGTTCGATGTTCAAAAAATTTCTTAACTGCCATCAAGAAAGTC
NM_013244.3       GTAATGCTTGAAGATGATGTTCGATGTTCAAAAAATTTCTTAACTGCCATCAAGAAAGTC
                  ************************************************************

HsCD00515815      ATTGCATCCCTAGAAGGAACTTACTGGGTAACTCTTGAATTCTCTAAGCTTGGCTACATT
NM_013244.3       ATTGCATCCCTAGAAGGAACTTACTGGGTAACTCTTGAATTCTCTAAGCTTGGCTACATT
                  ************************************************************

HsCD00515815      GGTAAACTCTATCATTCTCATGATCTCCCACGTTTGGCCCATTTTTTATTAATGTTTTAT
NM_013244.3       GGTAAACTCTATCATTCTCATGATCTCCCACGTTTGGCCCATTTTTTATTAATGTTTTAT
                  ************************************************************

HsCD00515815      CAAGAAATGCCTTGTGATTGGCTATTGACTCATTTCCGTGGTCTGTTGGCTCAGAAAAAT
NM_013244.3       CAAGAAATGCCTTGTGATTGGCTATTGACTCATTTCCGTGGTCTGTTGGCTCAGAAAAAT
                  ************************************************************

HsCD00515815      GTGATCCGTTTTAAACCATCTCTCTTTCAGCACATGGGCTATTATTCATCATACAAAGGG
NM_013244.3       GTGATCCGTTTTAAACCATCTCTCTTTCAGCACATGGGCTATTATTCATCATACAAAGGG
                  ************************************************************

HsCD00515815      ACGGAGAATAAGCTGAAGGATGATGATTTTGAAGAGGAGTCATTTGACATTCCTGATAAC
NM_013244.3       ACGGAGAATAAGCTGAAGGATGATGATTTTGAAGAGGAGTCATTTGACATTCCTGATAAC
                  ************************************************************

HsCD00515815      CCCCCTGCAAGTCTGTACACCAACATGAATGTGTTTGAAAATTATGAAGCAAGCAAGGCT
NM_013244.3       CCCCCTGCAAGTCTGTACACCAACATGAATGTGTTTGAAAATTATGAAGCAAGCAAGGCT
                  ************************************************************

HsCD00515815      TACAGTAGTGTTGATGAGTACTTTTGGGGGAAACCACCTTCAACAGGAGATGTTTTTGTG
NM_013244.3       TACAGTAGTGTTGATGAGTACTTTTGGGGGAAACCACCTTCAACAGGAGATGTTTTTGTG
                  ************************************************************

HsCD00515815      ATTGTATTTGAAAATCCAATTATAATAAAAAAAATTAAAGTAAATACTGGAACAGAAGAT
NM_013244.3       ATTGTATTTGAAAATCCAATTATAATAAAAAAAATTAAAGTAAATACTGGAACAGAAGAT
                  ************************************************************

HsCD00515815      CGGCAAAATGATATTTTGCATCATGGAGCCCTAGATGTTGGGGAAAACGTTATGCCTAGC
NM_013244.3       CGGCAAAATGATATTTTGCATCATGGAGCCCTAGATGTTGGGGAAAACGTTATGCCTAGC
                  ************************************************************

HsCD00515815      AAACAAAGGAGACAATGTTCTAGTTACTTAAGACTAGGAGAATTCAAAAATGGAAACTTT
NM_013244.3       AAACAAAGGAGACAATGTTCTACTTACTTAAGACTAGGAGAATTCAAAAATGGAAACTTT
                  ********************** *************************************

HsCD00515815      GAAATGTCAGGTGTAAATCAAAAAATTCCATTTGATATACATTGTATGAGGATATATGTC
NM_013244.3       GAAATGTCAGGTGTAAATCAAAAAATTCCATTTGATATACATTGTATGAGGATATATGTC
                  ************************************************************

HsCD00515815      ACCAAAACACAAAAGGAATGGCTAATTATTAGGAGTATTAGCATTTGGACTTCT---
NM_013244.3       ACCAAAACACAAAAGGAATGGCTAATTATTAGGAGTATTAGCATTTGGACTTCTTAG
                  ******************************************************

Pathways

Reactome :

Asparagine N-linked glycosylation

Reactome :

Metabolism of proteins

Reactome :

N-Glycan antennae elongation

Reactome :

N-glycan antennae elongation in the medial/trans-Golgi

Reactome :

Post-translational protein modification

Reactome :

Transport to the Golgi and subsequent modification

Protein

HPRD :

09575

Clone

Cloning Information :

669294

Original Clone ID :

ccsbBroadEn_07949

Organism

Species Specific ID:

25834

Genome

Chromosome :

Map Location :

12q21

Ensembl :

ENSG00000182050

Reagents

Labome :

UDP-N-acetylglucosamine-alpha-1-3-D-mannoside-beta